Department:
Chemistry
Date SOP was written:
11/26/2012
Date SOP was approved by PI/lab supervisor:
Office Phone:
1/13/2013
Principal Investigator:
Lab Phone:
Richmond Sarpong
Internal Lab Safety Coordinator/Lab Manager:
Rebecca Murphy
510-643-2485
510-643-6312
Emergency Contact:
Location(s) covered by this SOP:
Richmond Sarpong 626-644-2407
(Name and Phone Number)
Latimer 834, 836, 837, 838, 839, 842, 844, 847, 849,
907
(Building/Room Number)
☐
☒
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Oxygen is a strong oxidizer. It is used in medical application to not only increases oxygen levels in the patient's blood, but has the secondary effect of decreasing resistance to blood flow in many types of diseased lungs, easing work load on the heart. Oxygen is also used in smelting of iron ore into steel by injection through a high-pressure lance into molten iron, which removes sulfur impurities and excess carbon as the respective oxides, SO
2
and CO
2
.
CAS#: 7782-44-7
Class: Strong Oxidizer
Molecular Formula: O
2
Form (physical state): Gas
Oxygen. 1
SOP Template developed by The UC Center for Laboratory Safety
Date: 9/12/2012
KS

Color: Clear, Colorless
Boiling point: -183 O C
Contact with rapid expanding gas could cause burns or frostbite. Contact with combustible material may cause a fire.
Respiratory Protection
Not applicable
NOTE: Lab personnel intending to use/wear a respirator mask must be trained and fit-tested by EH&S.
This is a regulatory requirement.
Hand Protection
Nitrile gloves are recommended.
NOTE: Lab-specific and chemical-specific information on glove selection may be included in the
Protocol/Procedure section. Refer to glove selection from the link below:
For glove selection, go to: http://ehs.berkeley.edu/hs/63-laboratory-safety/94-glove-selection-andusage.html
Eye Protection
ANSI approved safety glasses or goggles.
Skin and Body Protection
Full-length pants, closed-toe shoes, and a lab coat.
Hygiene Measures
Avoid contact with pressurized gas streams with skin, eyes and clothing. Wash hands before breaks.
Work with oxygen should be conducted in a fume hood unless other controls are designated in the labspecific Protocol/Procedure section
In case of skin contact
Frostbite may occur, try to warm up the frozen tissues and seek medical attention.
In case of eye contact
In the case of Frostbite or particles in eye from pressurized gas stream, immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Get medical attention immediately.
Handling
Oxygen. 2
SOP Template developed by The UC Center for Laboratory Safety
Date: 9/12/2012
KS

High pressure gas. Do not puncture or incinerate container. Use equipment rated for cylinder pressure.
Close valve after each use and when empty. Store in tightly-closed container. Avoid contact with combustible materials. Protect cylinders from physical damage; do not drag, roll, slide, or drop. Use a suitable hand truck for cylinder movement. On oxygen cylinder fittings use no grease or lubricants..
Storage
Keep container tightly closed. Keep container in a cool, well-ventilated area. Separate from acids, alkalis, reducing agents and combustibles. Cylinders should be stored upright, with valve protection cap in place, and firmly secured to prevent falling or being knocked over. Cylinder temperatures should not exceed 52
°C (125 °F). Store at least 20 feet away from flammable compressed gas cylinders. Must be secured with double chains, 1/3 from the top and bottom.
911
Spill – Assess the extent of danger from fire or potential explosion. Help contaminated or injured persons. Evacuate the spill area
Chemical Spill on Body or Clothes – Not applicable for the gas, Any major release evacuate the area until the risk of fire or explosion hazard has been dissipated. N otify supervisor and EH&S immediately.
Chemical Splash Into Eyes – Immediately rinse eyeball and inner surface of eyelid with water from the emergency eyewash station for 15 minutes by forcibly holding the eye open. Seek medical attention.
Notify supervisor and EH&S immediately.
911
Life Threatening Emergency, After Hours, Weekends And Holidays – Dial 911 or contact the nearest emergency room. Note: All serious injuries must be reported to EH&S within 8 hours. Follow up with a call to 510-642-9090 to report the incident.
Non-Life Threatening Emergency – Go to the Occupational Health Facility (OHF), CHS Room. At all other times report to the nearest emergency room. Note: All serious injuries must be reported to EH&S within 8 hours.
Follow up with a call to 510-642-9090 to report the incident.
Needle stick/puncture exposure (as applicable to chemical handling procedure) – Wash the affected area with antiseptic soap and warm water for 15 minutes. For mucous membrane exposure, flush the affected area for 15 minutes using an eyewash station. Page the needle stick nurse. At all other times report to the nearest emergency room. Note: All needle stick/puncture exposures must be reported to
EH&S within 8 hours.
Follow up with a call to 510-642-9090 to report the incident.
When the oxygen compressed gas cylinder is empty, remove any attachments from the valve, cap the cylinder, and mark “empty” on the cylinder.
General hazardous waste disposal guidelines:
Label Waste
Oxygen. 3
SOP Template developed by The UC Center for Laboratory Safety
Date: 9/12/2012
KS


Affix an on-line hazardous waste tag on all waste containers using the Online Tag Program http://otp.ucop.edu/ as soon as the first drop of waste is added to the container
Store Waste

Not applicable
Dispose of Waste

When the oxygen compressed gas cylinder is empty, remove any attachments from the valve, cap the cylinder, and mark “empty” on the cylinder.

SDS can be accessed online at http://ucmsds.com
Chemical Storage
Preparation
Chemical Disposal
Oxygen at standard temperature and pressure is a tasteless gas. It is a strong oxidant and thus HIGHLY flammable when flames and flammable materials are present!!!
Conditions to Avoid: Avoid sunlight, heat, flames, sparks and other sources of ignition
Incompatibility: phosphorus, strong reducing agents, metals
Store in a cool, dry, well-ventilated area away from incompatible substances.
Keep cylinders secured and in an upright position at all times.
Gas cylinders should be closed after each use.
Post a no smoking/no open flame signs near the cylinder.
Oxygen should ONLY be stored in approved gas cylinders.
Eliminate any sources of heat (see above) from the work area.
Clearly ask coworkers in the same room if they are working with open flames, hydrogen, or strong volatile reducing agents.
Know the location of the nearest fire extinguisher before beginning work.
Return empty oxygen cylinders to the distributer.
Excess oxygen should NEVER be vented near open flames and should always be vented in a fume hood. Oxygen at elevated partial pressures can be toxic
(beginning at approximately 50 kPa).
Oxygen. 4
SOP Template developed by The UC Center for Laboratory Safety
Date: 9/12/2012
KS

Procedure/Use
1.
Transferring
Oxygen From
Cylinder to Balloon for Reactions
2. Using balloon oxygen from reactions.
3. Venting oxygen
Scale
Up to 1/2 standard party balloon volume (0.25 cubic feet or approx 7 liters).
Engineering
Controls/Equipment
Oxygen should only be obtained from an approved oxygen cylinder equipped an appropriate oxygen regulator.
Reactions using oxygen should only be performed in a fume hood.
Balloons should be doubled as a measure against the balloon popping. This is achieved by taking one standard balloon and placing it inside of another, minimizing the amount of air in the areas between the two balloons.
Balloons should be sealed to an open/close valve needle adaptor and sealed with a rubber band and Parafilm.
All work using oxygen must be performed in a ventilated fume hood.
PPE (eye, face, gloves, clothing)
Eye Protection:
Wear tight-fitting safety goggles or safety glasses with side shields.
Face :
No face protection needed.
Protection
Gloves:
Use standard reusable nitrile, rubber, or neoprene gloves.
Clothing:
Wear flame resistant lab coat, full length pants, closed toed shoes.
Procedure Steps and
Precautions
Precaution:
Oxygen in the presence of a flame and fuel is highly flammable. The presence of oxygen accelerates fires.
Oxygen at elevated partial pressures can be toxic
(beginning at approximately 50 kPa).
Keep oxygen away from flames, sparks and flammable materials.
Procedure: Ensure no sparks or open flames are present.
With all regulators outlets closed, open gas cylinder connected to regulator slowly.
Ensure there is properly fitted and sealed rubber septum on one of the regulator outlets.
Using a standard party ballon setup (see engineering controls and equipment section ) switch the valve to open and then place the needle in the septum.
Slowly open the regulator valve leading to the septum. Oxygen will then fill the balloon. FILL
THE BALLOON ONLY TO ½
THE MAXIMUM VOLUME.
Switch the balloon valve to closed, close the regulator valve, and then the oxygen tank. Vent the regulator to remove oxygen from it.
Place needle of on/off valve needle adaptor into the septum of your reaction. Switch the valve to OPEN.
Carefully vent small amounts of oxygen in a fume hood by leaving the on/off valve needle adaptor to the on position, and letting the balloon deflate.
Oxygen. 5
SOP Template developed by The UC Center for Laboratory Safety
Date: 9/12/2012
KS

Any deviation from this SOP requires approval from PI.
(signature of all users is required)

Prior to conducting any work with oxygen, designated personnel must provide training to his/her laboratory personnel specific to the hazards involved in working with this substance, work area decontamination, and emergency procedures.

The Principal Investigator must provide his/her laboratory personnel with a copy of this SOP and a copy of the SDS provided by the manufacturer.

The Principal Investigator must ensure that his/her laboratory personnel have attended appropriate laboratory safety training or refresher training within the last one year.
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Oxygen. 6
SOP Template developed by The UC Center for Laboratory Safety
Date: 9/12/2012
KS

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Oxygen. 7
SOP Template developed by The UC Center for Laboratory Safety
Date: 9/12/2012
KS