SUPPORTING INFORMATION
Figure S1. Schematic representation of the T-DNA insertion in the mutants for SUS1 (N544615) and SUS4
(N619660). The promoter region is represented in blue, the UTR regions in grey, the exons in black. LB
indicates the left border of the T-DNA sequence.
Figure S2. Microarray analysis of the six sucrose synthase genes using the Genevestigator Tool. Datasets
related to low-oxygen treatments were selected.
Figure S3. Microarray analysis of the nine invertase genes using the Genevestigator Tool. Datasets related to
low-oxygen treatments were selected.
Table S1. Growth media, conditions, treatments used for the experiments reported in the figures.
Figure
Growth media
Growth
conditions
treatment
Media composition
during the treatment
1
Multi well with liquid media,
Sucrose 90mM
4 days old
seedlings,
dark grown
anoxia,
hypoxia and
submergence,
48 h, darkness
Sucrose 90mM
2a
Multi well with liquid media,
Sucrose 0 - 22,5- 45-90 mM
4 days old
seedlings,
dark grown
Anoxia, 48 h,
darkness
Sucrose 0 - 22,5- 45-90
mM
2.b
Multi well with liquid media,
Sucrose 90 mM or
Glucose 90 mM+Fructose 90
mM
4 days old
seedlings,
dark grown
Anoxia, 48 h,
darkness
Sucrose 90 mM or
Glucose 90
mM+Fructose 90 mM
2.c
Multi well with liquid media,
Sucrose 90mM
4 days old
seedlings,
dark grown
2.d
Multi well with liquid media,
Sucrose 90mM
4 days old
seedlings,
dark grown
3.a
Vertical plates, 1% agar, sucrose
30mM
3.b
Vertical plates, 1% agar, sucrose
30mM
3.c
Glass jars, 1% agar, sucrose
30mM
4.a-b
Pot grown plants (in soil)
4.c
Hydroponic system with
nutrient solution
4.d
Hydroponic system with
nutrient solution
4.e-g
Hydroponic system with
nutrient solution
5.a
5.b
Multi well with liquid media,
glucose 55mM and sucrose
15mM
Multi well with liquid media,
glucose 55mM and sucrose
15mM
2 weeks,
12:12
photoperiod
2 weeks,
12:12
photoperiod
2 weeks,
12:12
photoperiod
3 weeks,
12:12
photoperiod
3 weeks,
12:12
photoperiod
3 weeks,
12:12
photoperiod
3 weeks,
12:12
photoperiod
anoxia,
hypoxia and
submergence,
48 h, darkness
anoxia,
hypoxia and
submergence,
48 h, darkness
anoxia 22h
and 24h,
darkness
hypoxia 5 and
7 days,
darkness
submergence
7 and 14 days,
darkness
waterlogging 4
days, 12:12
photoperiod
waterlogging
3,6,and 8 days,
12:12
photoperiod
waterlogging
24 and 48 h at
light
Recovery
Sucrose 90mM
Sucrose 90mM
1% agar, sucrose
30mM
1 week
1% agar, sucrose
30mM
1 week
1% agar, sucrose
30mM
1 week
1 week
waterlogging
48 h at light
4 days, 12:12
photoperiod
anoxia 12h
and 24h,
darkness
mannitol 70mM
4 days, 12:12
photoperiod
anoxia 24h,
darkness
mannitol 70mM
Table S2. Data were subjected to one-way and two-way analysis of variance (ANOVA) and the means were
separated using the F-test (95.0% confidence level). Significance of two-way analysis of variance (*P-value
≤ 0.05; ***P-value ≤ 0.001;n.s. = not significant):
Fig.2
(2.a)
Ethanol quantification for different genotype (a) : n.s.; air/anoxia treatment (b) : ***.; a x b: n.s.
(2.b)
Ethanol quantification for different genotype (a) : *.; air/anoxia treatment (b) : *** ; a x b: n.s.
(2.c)
Ethanol quantification for different genotype (a) : *.; air/anoxia treatment (b) : ***.; a x b: *;
Ethanol quantification for different genotype (a) : n.s.; air/hypoxia treatment (b) : ***.; a x b: n.s.
Ethanol quantification for different genotype (a) : n.s.; air/submergence treatment (b) : ***.; a x b:
n.s.
(2.d)
ATP/ADP ratio for different genotype (a) : n.s.; air/anoxia treatment (b) : ***.; a x b: n.s.
ATP /ADP ratio for different genotype (a) : n.s.; air/hypoxia treatment (b) : ***.; a x b: n.s.
ATP/ADP ratio for different genotype (a) : n.s.; air/submergence treatment (b) : ***.; a x b: n.s.
Fig.4
(4.b)
Rosette area in waterlogged plants (a): ***; air/ waterlogging treatment (b) : ***; a x b: ***.
(4.d)
n.s.
Ethanol quantification for different genotype (a) : n.s.; air/ waterlogging treatment (b) : *.; a x b:
(4.e)
ATP/ADP ratio for different genotype (a) : n.s.; air/ waterlogging treatment (b) : ***.; a x b: n.s.
(4.f)
INV activity for different genotype (a) : ***; air/ waterlogging treatment (b) : *; a x b: *;
(4.g)
SUS activity for different genotype (a) : n.s.; air/ waterlogging treatment (b) : ***.; a x b: n.s.
Fig 5
(5.c)
ATP/ADP ratio for different genotype (a) : n.s.; treatment (b) : ***.; a x b: n.s.