Curriculum Vitae
Surname: Antopolsky
First Name: Maxim
E-mail: Maxim.Antopolsky@helsinki.fi
Address: Hahaniemenranta 26A, 40, 00530 Helsinki, Finland
Phone: +358 40 578 31 58

married, wife – Elizarova Tatiana (14.09.1960), daughter – Antopolskaya
Ksenia (08.03.1991)
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I was born on 18.09.1961 in Moscow.
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In 1969 entered a special school number 9 in Moscow (English language) and
in 1979 graduated.

In 1979 entered the 1st Moscow Medical Sechenov Institute, Pharmaceutical
Faculty, and in 1984 graduated getting a degree master of science.

1984-1987 was working as a head of the department of Diagnostic Drugs in
the Central Moscow Drug Store.

1987-1989 was working in the Laboratory of Heterocyclic Compounds in the
Moscow Vitamin Institute as a researcher (synthesis, isolation and purification
of coenzyme A and its analogues).

1989-1991 was working as a researcher in the Laboratory of Peptide Synthesis
in the Institute of Experimental Cardiology (Moscow Cardiology Centre) in
the field of solid phase and in solution synthesis of amino acid derivatives,
peptides, their isolation, purification and characterization.

1992-1993 got a Postdoctoral position in the department of Peptides and
Nucleic Acids (Hoechst, Frankfurt-am-Main) and was working in the field of
synthesis, isolation, purification and characterization of unnatural amino acids,
their derivatives and peptide analogues.

1993-1997 was working as a senior researcher in the Laboratory of Peptide
Synthesis in the Institute of Experimental Cardiology (Moscow Cardiology
Centre) in the field of solid phase and in solution synthesis of amino acid
derivatives, peptides, cyclic peptides their isolation, purification and
characterization.

1997-2002 a researcher in the department of Pharmaceutical Chemistry,
University of Kuopio, Finland. Research field is synthesis of peptideoligonucleotide conjugates, isolation, purification and characterization of
those novel compounds.
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27 September 2002 defended PhD dissertation “Synthesis of peptideoligonucleotide conjugates” and got Ph. Doctor Degree.

29.10. 2003 got a Ph. Doctoral degree. 2003-2006 senior researcher in the
department of Pharmaceutical Chemistry, University of Kuopio, Finland.
Research field is synthesis of peptides, modified olygonucleotides, peptideoligonucleotide conjugates and conjugate libraries, development of novel
supports, reagents and methods for solid phase synthesis of oligonucleotides
and peptide-oligonucleotide conjugates; isolation, purification and
characterization of those novel compounds. During last years I dedicated a lot
of my activity to the development of novel solid supports suitable either for
solid phase stepwise synthesis of peptide-oligonucleotide conjugates or serve
as polymeric universal supports for DNA / RNA solid phase synthesis. That
work ended up with patent and patent applications mentioned in the list of my
publications (11, 12, 15, 16). During last four years I kept giving a lecture and
practical course on peptide synthesis for graduate and post graduate students
of Kuopio University and supervise two PhD students in the area of solid
phase synthesis.

1.08.2006 I’ve got a position in the group of Prof. Arto Urtti, DDTC, faculty
of Pharmacy, University of Helsinki. I started the research in the field of
structure – activity relations in cell penetrating peptides and development of
peptide-based systems for gene delivery and cell targeting, trying to apply my
chemical skills in the field of biopharmacy. From 2007 my field of interest
also include design, synthesis and property studies of surfactant like peptides.
Synthesis, isolation and purification of different compounds of oligonucleotide
nature, such as primers, siRNA, antisense oligonucleotides, preparation of
monomers and building blocks for solid phase oligonucleotide synthesis are
also a part of my research duties.

From 1.032008 I’ve got a position of a group leader in CDR (Centre for Drug
Research, University of Helsinki) leaded by Professor Arto Urtti. In addition
to the topics mentioned starting from this time my research field also includes
synthesis of nanoparticles and modification of their surface with different
peptides displaying cell penetrating and targeting properties in order to adjust
targeted delivery of gene and gene related materials. From the year 2008 I also
act as an academic editor in PlosONE journal.
List of Publications
of PhD Maxim Antopolsky
1. Sidorova M.V., Kudriavtseva E.V., Antopol’skii M.L., Pal’keeva M.E.,
Ovchinnikov M.V., Bespalova Zh.D. Hydrogen peroxide for disulfide bridge
formation in the synthesis of peptides with the sequence of the
immunodominant epitope of HIV gp41 glycoprotein.
Bioorg Khim. 1996 Apr;22(4):273-9. Russian
2. . M. Antopolsky, ,. E. Azhayeva, U. Tengvall,, S. Auriola, I. Jääskeläinen, S.
Rönkkö, P. Honkakoski, A. Urtti, H. Lönnberg, A. Azhayev, A. “PeptideOligonucleotide onjugates with Membrane Translocation and Nuclear
Localization Properties” Bioconjugate Chem. (1999) vol. 10, pp. 598-606.
3. M. Antopolsky, A. Azhayev “Stepwise Solid-Phase Synthesis of PeptideOligonucleotide Conjugates on New Solid Supports” Helvetica Chimica Acta
(1999), vol. 82, pp. 2130-2140.
4. M. Antopolsky, A. Azhayev in Perspectives in Nucleoside and Nucleic Acid
Chemistry, M.V.Kisakurek, H.Rosemeyer Eds., WILEY-VCH, 2000, pp. 275285.
5. M. Antopolsky, A. Azhayev “Stepwise Solid-Phase Synthesis of PeptideOligonucleotide Phosphorothioate Conjugates employing Fmoc peptide
chemistry” Tetrahedron Lett. (2000), vol. 41, pp. 9113-9117.
6. M. Antopolsky, A. Azhayev.” Synthesis of Peptide-Oligonucleotide
Phosphorothioate Conjugates by convergent or stepwise solid-phase
strategies” Nucleosides, Nucleotides and Nucleic Acids (2001), vol. 20, pp.
539-550.
7. A.Azhayev, M. Antopolsky “Amide-group assisted 3’-deposphorylation of
oligonucleotides synthesized on universal A-supports”, Tetrahedron (2001),
vol. 57/23, pp 4977-4986.
8. M. Antopolsky, E.Azhayeva, U. Tengvall, A. Azhayev “Towards a General
Method for
the Stepwise solid-phase synthesis of peptide-oligonucleotide conjugates”
Tetrahedron Lett. (2002), vol. 43, pp. 527-530.
9. Antopolsky, Maxim L.; Roivainen, Jarkko; Randolph, John; Mackie, Hugh; Azhayev,
Alex. Improving the universal solid support for oligonucleotide synthesis.
Innovation and Perspectives in Solid Phase Synthesis & Combinatorial Libraries:
Peptides, Proteins and Nucleic Acids--Small Molecule Organic Chemistry Diversity,
Collected Papers, International Symposium, 7th, Southampton, United Kingdom,
Sept. 18-22, 2001 (2002), Meeting Date 2001, 51-54. CODEN: 69DYT7 CAN
140:164108 AN 2003:425693 CAPLUS.
10. Antopolsky M, Azhayeva E, Tengvall U, Azhayev A: Geeneral method to
synthesize disulfide cross-linked peptide-oligonucleotide conjugates.
Collection Symposium Series 2: 294-297, 1999.
11. A.Azhayev, M. Antopolsky "Universal supports for oligonucleotide
Synthesis" United States Patent Serial No 6.770,754 and European Patent
No 1404695L.
12. Beigelman, A.Azhayev, E.Azhayeva, M.Antopolsky “Enzymatic Nucleic
Acid Peptide Conjugates” (2002), United States Patent Application No
60/306,995.
13. M.Antopolsky “Synthesis of Peptide-Oligonucleotide Conjugates” Doctoral
Dissertation (2002) Kuopio University Printing Office.
14. U. Tengvall, S. Auriola, M. Antopolsky, A. Azhayev, L. Biegelman
“Characterization of antisense oligonucleotide-peptide conjugates with
negative ionization alectrospray mass spectrometry and liquid chromatography
– mass spectrometry” Jornal of Pharmaceutical and Biomedical Analisis
(2003) vol. 32, pp 581-590.
15. Antopolsky, M., Azhayeva, E., Tengvall, U., Azhayev, A. "Stepwise
Solid-phase synthesis of Peptide-oligonucleotide conjugates and supports
therefore". (2003) United States Patent Application No 10/242,668.
16. Alex Azhayev and Maxim Antopolsky “Universal supports for oligonucleotide
synthesis” (2004) United States Patent Application No 10/851,289.
17. A.V. Azhayev, M.L. Antopolsky, T.M.L. Tennilä, H. Mackie, J.B. Randolpg “
A Comparative Study of Commercially Available Universal Supports”
Genetic Engeneering News (2005) vol 25, n. 5.
18. Raula, Janne; Kuivanen, Annukka; Laehde, Anna; Jiang, Hua; Antopolsky,
Maxim; Kansikas, Jarno; Kauppinen, Esko I. Synthesis of -leucine
nanoparticles via physical vapor deposition at varying saturation conditions.
Journal of Aerosol Science (2007), 38(12), 1172-1184
19. Yagodkin, Andrey; Azhayev, Alex; Roivainen, Jarkko; Antopolsky, Maxim;
Kayushin, Alexei; Korosteleva, Maria; Miroshnikov, Anatoly; Randolph,
John; Mackie, Hugh. Improved Synthesis of Trinucleotide Phosphoramidites
and Generation of Randomized Oligonucleotide Libraries. Nucleosides,
Nucleotides & Nucleic Acids (2007), 26(5), 473-497.
20. Azhayeva, Elena; Tennilä, Tuula; Azhayev, Alex; Antopolsky, Maxim,
Peptide-oligonucleotide Conjugates Form Stable and Selective Complexes
with Antibody and DNA submitted in Bioconjugate Chemistry.
21. Ranta, Veli-Pekka; Mannermaa, Eliisa; Lummepuro, Kirsi; Subrizi, Astrid; Laukkanen,
Antti; Antopolsky, Maxim; Murtomaeki, Lasse; Hornof, Margit; Urtti, Arto. Barrier
analysis of periocular drug delivery to the posterior segment. Journal of
Controlled Release (2010), 148(1), 42-48.