Cryobiology
Note: Please look through resources very carefully. This is a controversial issue so some sources may have bias. Check
carefully for the expertise or background of the writer or contributor.
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General Sources
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Society for Cryobiology
Society for Low Temperature Biology
CRYOBIOLOGY
CryoLetters
Cell Preservation Technology
Cellular cryobiology and anhydrobiology
An overview of the science behind cryobiology at the Science Creative Quarterly
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Scientist finds fungus gnats survive winter half-frozen | Alaskana | ADN.com
Alaska beetles survive 'unearthly' temperatures
Nation & World | Looking to frozen frogs for clues to improve human medicine | Seattle Times Newspaper
http://virgil.ruc.dk/~cryolab/research.html
Cryobiology
"Freeze Tolerance as an Overwintering Adaptation in Cope's Grey Treefrog (Hyla chrysoscelis)".
Barnes, Brian M. (30 June 1989). "Freeze Avoidance in a Mammal: Body Temperatures Below 0 °C in an Arctic
Hibernator" (PDF). Science (American Association for the Advancement of Science) 244 (4912): 1521–1616.
doi:10.1126/science.2740905. PMID 2740905. Retrieved 2008-11-23.
C. Polge, AU Smith, AS Parkes,Revival of spermatozoa Effective vitrification and dehydration at low temperatures
Nature, 164 (1949), p. 666
Raison JK (1973). "The influence of temperature-induced phase changes on the kinetics of respiratory and other
membrane-associated enzyme systems". J Bioenerg 4 (1): 285–309. doi:10.1007/BF01516063. PMID 4577759.
Mühlbacher F, Langer F, Mittermayer C (1999). "Preservation solutions for transplantation". Transplant Proc 31 (5):
2069–70. doi:10.1016/S0041-1345(99)00265-1. PMID 10455972.
Hunt CJ, Armitage SE, Pegg DE (2003). "Cryopreservation of umbilical cord blood: 1. Osmotically inactive volume,
hydraulic conductivity and permeability of CD34(+) cells to dimethyl sulphoxide". Cryobiology 46 (1): 61–75.
doi:10.1016/S0011-2240(02)00180-3. PMID 12623029.
"Freezing". Pacific Fertility Center. 2010. Retrieved 2010-02-28.
Darwin, Mike (1991). "Cold War: The Conflict Between Cryonicists and Cryobiologists". Cryonics (Alcor Life Extension
Foundation). Retrieved 2009-08-24.
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Cryobiology
Cryonics
1. An Exploratory Survey Examining the Familiarity With and Attitudes Toward Cryonic Preservation - A paper
examining the results of a consumer survey on cryonics. By W. Scott Badger, from "The Journal of
Evolution and Technology," December, 1998.
2. Anders Sandberg's Cryonics Page - An extensive list of links to cryonics articles and to European sites
related to cryonics.
3. Brain Death and Technological Change: Personal Identity, Neural Prostheses and Uploading - A far-seeing
but technical paper by James J. Hughes, prepared for the Second International Symposium on Brain Death.
Date unclear, but 1996 or later. Includes discussion of cryonics and nanotechnology.
4. Can Cryopreservation Solve the Abortion Problem? - A thoughtful article by Thomas Gramstad which
examines the social implications of preserving aborted embryos. From Longevity Report.
5. Cryonics -- Frequently Asked Questions (FAQ) - Up-to-date answers to basic cryonics questions. A good
starting point. By Ben Best.
6. Cryonics Europe - Support and discussion group based in Sussex, UK, for people in Britain and the rest of
Europe who are signed up for cryopreservation.
7. Cryonics and Cryptography - "Cryonics, Cryptography, and Maximum Likelihood Estimation," a paper by
Ralph Merkle, Ph.D.
8. Depressed Metabolism: What is Cryonics? - Article by Aschwin de Wolf about using low temperatures to
care for the critically ill.
9. Frozen Dead Guy Days - The official site of a winter festival held annually in march in the mountain town of
Nederland, Colorado. A bit weird, since the Nederlands city government actively worked to oust Bredo
Morstoel (the "frozen dead guy") from their town several years ago. But there he still sits, now the frozen
centerpiece of a festival.
10. IceLift - Human hibernation project. Offers the possibility of preserving all body tissues in an unchanged
quality for a long time, in the expectation that the world will be a better place to live 50 years from now.
Multilingual.
11. Longevity Report - John DeRivaz's British site for cryonics, life extension, and the future.
12. The Cryonics Society - A non-profit organization that aims to educate and inform the general public about
cryonics. Does not provide cryonics services and is not affiliated with any service organization.
13. Would Freezing Ted Williams Work? - From ABCNEWS.com, an article on the cryonic suspension of Ted
Williams by the Alcor Foundation and asking if the technology might work. (July 09, 2004)
14. Cryonics Over Dead Geeks' Bodies - Wired News article by Michelle Delio, based on an interview with
Heather Pringle, author of the new book: "The Mummy Congress: Science, Obsession and the Everlasting
Dead". (July 20, 2001)
DMSO Cryoprotectant
1. Novak, K. M., ed. (2002). Drug Facts and Comparisons (56th ed.). St. Louis, Missouri: Wolters Kluwer
Health. p. 619. ISBN 1-57439-110-0.
2. 187 fake cancer "cures" to avoid, FDA
3. DMSO, National Council Against Health Fraud
Cryobiology
4. Kvakovszky G, McKim AS, Moore J. (2007). "A Review of Microelectronic Manufacturing Applications Using
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DMSO-Based Chemistries". ECS Transactions 11 (2): 227–234. doi:10.1149/1.2779383.
Epstein WW, Sweat FW (March 1967). "Dimethyl Sulfoxide Oxidations". Chemical Reviews 67 (3): 247–260.
doi:10.1021/cr60247a001. PMID 6042131.
Tidwell TT. (1990). "Oxidation of Alcohols by Activated Dimethyl Sulfoxide and Related Reactions: An
Update". Synthesis 1990 (10): 857–870. doi:10.1055/s-1990-27036.
Pegg, DE. (2007). "Principles of Cryopreservation". In Day JG, Stacey GN. Cryopreservation and FreezeDrying Protocols, Second Edition. Methods in Molecular Biology (Humana Press) 368: 39–57.
doi:10.1007/978-1-59745-362-2_3. ISBN 978-1-58829-377-0. ISSN 1064-3745. PMID 18080461.
Johannes Geiss (2001). The century of space science. Kluwer Academic. p. 20. ISBN 0-7923-7195-X.
Retrieved 2011 Aug 7.
Shirley SW, Stewart BH, Mirelman S. (March 1978). "Dimethyl Sulfoxide in Treatment of Inflammatory
Genitourinary Disorders". Urology 11 (3): 215–220. doi:10.1016/0090-4295(78)90118-8. PMID 636125.
Kelava T, Cavar I (Nov 2011). actions of drug solvents) "Biological actions of drug solvents". Periodicum
Biologorum 113 (3): 311–320.
Kelava T, Cavar I (Oct 2010). "Influence of small doses of various drug vehicles on acetaminophen-induced
liver injury". Can J Physiol Pharmacol 88 (10): 980–87. doi:10.1139/Y10-065. PMID 20962895.
DMSO information from American Cancer Society
DMSO at WebMD
Brisbane drug company convicted of counterfeiting, Therapeutic Goods Agency
Carley W. (September 9, 1965). "DMSO may have caused death of woman, makers of 'Wonder' drug warn
doctors". Wall Street Journal (New York City).
FDA: http://www.fda.gov/ForIndustry/ImportProgram/ImportAlerts/ucm162294.htm
DMSO Cryopreservation
1. Cryopreservation and Storage of Cells | Sigma-Aldrich
2. DMSO - Cryopreservation Comes With a Cost - The Cell Culture Dish
3. Use of different DMSO concentrations for cryopreservation of ...
4. Re: How DMSO protects cells upon freezing?
5. Long term cryopreservation in 5% DMSO maintains unchanged ...
6. General Protocol for the Cryopreservation of Mammalian Cells ...
7. Cryopreservation Technical Manual - ATCC
8. DMSO-Free Programmed Cryopreservation of Fully Dissociated and ...
9. Cryopreservation of Cell Lines - OPS Diagnostics
10. Cryopreservation
11. Freezing Cells | Life Technologies
12. Cryopreservation of hematopoietic stem cells: how did we get here ...
13. BioLife's CryoStor vs DMSO/Dextran (Umbilical Cord Blood)
14. Improving Cryopreservation Yield - BioLife Solutions
15. Why use DMSO and FCS for freezing cells? - Cell Biology - Protocol ...
16. Cryopreservation Hematopoietic Progenitor Cells - CBMTG
17. Cryopreservation Technical Manual - ATCC
18. Cryopreservation of Plant Genetic Resources - InTech
19. Cryopreservation Manual - Emory Environmental Health and Safety ...
Cryobiology
20. Cryopreservation of Sugarbeet (Beta Vugaris L.) Germplasm
21. 1 Reed, BM Chapter 3. Pretreatment strategies for - US Department ...
22. 1 Development and large scale application of cryopreservation ...
23. How to Cryopreserve Plant Cells | eHow
24. Cryopreservation | Cryopreservation information, tips, protocols, and ...
25. Cryopreservation of Brassia rex Orchid Hybrid Using DMSO ...
26. Popov, et al. "Patterns Of ISSR And REMAP DNA Markers After Cryogenic Preservation Of Spring Wheat Calli By
Dehydration Method." Russian Journal Of Plant Physiology 58.3 (2011): 423-430. Academic Search Premier. Web. 17
Sept. 2013. http://search.ebscohost.com/login.aspx?direct=true&db=aph&AN=60392489&site=ehost-live
27. POWELL, ERIC A. "Ice Age Flower Blooms Again." Discover 33.6 (2012): 13. Academic Search Premier. Web. 17 Sept.
2013. http://search.ebscohost.com/login.aspx?direct=true&db=aph&AN=76454103&site=ehost-live
28. Kholina, A. B., and N. M. Voronkova. "Conserving The Gene Pool Of Far Eastern Plants By Means Of Seed
Cryopreservation." Biology Bulletin 35.3 (2008): 262-269. Academic Search Premier. Web. 17 Sept. 2013.
http://search.ebscohost.com/login.aspx?direct=true&db=aph&AN=32090254&site=ehost-live
29. Karsten Eilertsen, et al. "Fertility Issues In Patients With Prostate Cancer." BJU International 102.7 (2008): 793-795.
Academic Search Premier. Web. 17 Sept. 2013.
http://search.ebscohost.com/login.aspx?direct=true&db=aph&AN=34081299&site=ehost-live
30. A. Popov, et al. "Cryopreservation Of Seeds And Protocorms Of Rare Temperate Orchids." Russian Journal Of Plant
Physiology 54.1 (2007): 121-127. Academic Search Premier. Web. 17 Sept. 2013.
http://search.ebscohost.com/login.aspx?direct=true&db=aph&AN=23635960&site=ehost-live
31. Bukhov, N. G., E. V. Popova, and A. S. Popov. "Photochemical Activities Of Two Photosystems In Bratonia Orchid
Protocorms Cryopreserved By Vitrification Method." Russian Journal Of Plant Physiology 53.6 (2006): 793-799.
Academic Search Premier. Web. 17 Sept. 2013.
http://search.ebscohost.com/login.aspx?direct=true&db=aph&AN=22911548&site=ehost-live
32. Bart Panis, et al. "Change In Sugar, Sterol And Fatty Acid Composition In Banana Meristems Caused By SucroseInduced Acclimation And Its Effects On Cryopreservation." Physiologia Plantarum 128.1 (2006): 80-94. Academic
Search Premier. Web. 17 Sept. 2013.
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33. JORG, BRIAN F. "Science Matters." Horticulture 108.3 (2011): 16-17. Academic Search Premier. Web. 17 Sept. 2013.
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34. Elstein, David. "The Germplasm Ambassador." Agricultural Research 53.11 (2005): 22. Academic Search Premier.
Web. 17 Sept. 2013. http://search.ebscohost.com/login.aspx?direct=true&db=aph&AN=19004142&site=ehost-live
Cryopreservation (Human and Animal) – LAPL card required
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3. Karlsson, Jens O.M. "Cryopreservation: freezing and vitrification." Science 296.5568 (2002): 655. Opposing
Viewpoints In Context. Web. 17 Sept. 2013.
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Cryobiology
4. Bonnicksen, Andrea L. "Embryo freezing: ethical issues in the clinical settings." The Hastings Center Report Dec.
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5. Sherwin, Susan. "Human Reproduction: Principles, Practices, Policies." The Hastings Center Report Mar.-Apr. 1995:
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