Abstract American Journal of Veterinary Research June 2011, Vol. 72, No. 6, Pages 746-750 doi: 10.2460/ajvr.72.6.746 Effect of topical vapocoolant spray on response to arthrocentesis and intravenous catheterization in unsedated horses Catherine T. Fjordbakk, DVM; Henning A. Haga, DVM, PhD Department of Companion Animal Clinical Sciences, Equine Teaching Hospital, Norwegian School of Veterinary Science, 0033 Oslo, Norway. (Fjordbakk, Haga) Address correspondence to Dr. Fjordbakk (cathrine.fjordbakk@nvh.no). Objective – To assess the efficacy of a commercially available topical vapocoolant spray in reducing responses to arthrocentesis of the middle carpal (MC) and metacarpophalangeal (MCP) joints and jugular vein catheterization in unsedated horses. Animals – 8 health research horses. Procedures – Arthrocentesis of both MC and MCP joints and bilateral jugular vein catheterization were performed in each horse. Immediately prior to skin penetration, 1 randomly selected MC joint, MCP joint, and jugular vein were sprayed with a vapocoolant liquid (intervention product), and the contralateral MC joint, MCP joint, and jugular vein were sprayed with water (placebo). An observer blinded to the type of spray treatment used evaluated the horses’ responses to needle or catheter placement procedures by use of a 6-point categorical scale and a 100-mm visual analog scale. Results – Responses evaluated via the visual analog scale were significantly reduced after application of the intervention product, compared with responses after application of the placebo, for the MC and MCP joints; no difference in responses to jugular vein catheterization was detected between the two treatments. Conclusions and Clinical Relevance – Vapocoolant spray was safe and effective in reducing horses’ responses to arthrocentesis. The use of such products prior to joint injections may reduce procedural nociception and pain anticipation in unsedated horses and may improve the safety of personnel performing such procedures.