ChE 380 – Introduction to Biotechnology Fall 2013 Instructor Kshitiz Chaudhary, Ph.D. E-mail: kc323@njit.edu Office Hours: By appointment only, Thu, 5:00 pm – 6:00 pm Catalog Description: ChE 380-101 – Introduction to Biotechnology. Prerequisites: Chem 122 or Chem 126. Basic principles of molecular biotechnology with selected examples of applications. These applications include, but are not limited to, the production of chemical and biological materials and the development of processes encountered in the Biotech industry. Textbook(s): 1. Introduction to Biotechnology, 3rd Ed. (2013), by William J. Thieman and Michael A. Palladino, Pearson/Benjamin Cummings, New York. Grading scheme: Project 20% Exam 40% Final 40% Policies: 1. Students are responsible for the material covered in class. Regular class attendance is expected. 2. Make-up exams and “incompletes” may be given under extraordinary circumstances and at the sole discretion of the instructor. Regular class attendance is expected. Schedule: Sept 5 Historical background - Chapter 1, Sections 1.1, 1.2 and 1.3 Sept 12 Cell structure - Chapter 2, Section 2.1 Microbial nutrition, growth, and metabolism - Lecture notes Sept 19 Introduction to genes and genomes - Chapter 2, Section 2.2: The Molecule of Life – DNA Structure - Chapter 2, Section 2.2: The Molecule of Life – What is a Gene? - Chapter 2, Section 2.3: What is a Genome? Sept 26 DNA replication, RNA and protein synthesis - Chapter 2, Section 2.3: DNA replication - Chapter 2, Section 2.4: RNA and protein Synthesis Oct 3 Mutations, genetic manipulation, DNA uptake, and applications - Chapter 2, Section 2.5 - Chapter 3, Section 3.1, Introduction to Recombinant DNA Technology and DNA Cloning Oct 10 Restriction enzymes/plasmids, gene cloning/analysis - Chapter 3, Section 3.1, Restriction Enzymes and Plasmid DNA Vectors - Chapter 3, Section 3.1, Bacterial transformation - Chapter 3, Section 3.3, Polymerase Chain Reaction Oct 17 Applications of Recombinant DNA Technology, Genomics and Bioinformatics - Chapter 3, Section 3.4, Gel Electrophoresis, DNA Sequencing, Gene Expression - Chapter 3, Section 3.5, Whole genome sequencing, Bioinformatics, Human genome project Oct 24 Protein as products - Chapter 4, Section 4.1, Proteins as products - Chapter 4, Section 4.3, Protein production MID-TERM EXAM Oct 31 Microbial biotechnology, microorganism, and applications - Chapter 5, Sections 5.3 - Lecture notes Nov 7 Microbial biotechnology, Vaccines, Micobial Genomes, Biofuels Chapter 5, Section 5.4, 5.5 and 5.6 Nov 14 Plant biotechnology and Applications - Chapter 6, Section 6.3 Animal biotechnology and applications - Chapter 7, Sections 7.2, 7.3 Nov 21 Medical Biotechnology - Chapter 11, Section 11.1, Detecting Human Diseases - Chapter 11, Section 11.3, 11.4, Gene Therapy, Stem Cell Technologies Dec 5 Examples from the Biotech industry - Lecture notes Dec 19 Final Exam The NJIT Honor Code and standards of academic integrity will be enforced in this course. Any violation will be brought to the immediate attention of the Dean of Students.