Curriculum Vitae Subha Das Personal Information Address: 2/37 Gerald Street, Lincoln 7608, Christchurch, Canterbury, New Zealand. Mobile: +64 - 210452363 Email: subhadas1981@gmail.com/subha.das@plantandfood.co.nz Date and place of birth: 08/05/1981, Kolkata, India Nationality: Indian Family status: Unmarried Education Since 04/2009 PhD studies at the New Zealand Institute for Plant and Food research, enrolled at Lincoln University (Bio-Protection Centre). Thesis title: “Rhizoctonia solani on potato in New Zealand: pathogen characterisation and identification of double-stranded RNA viruses that may affect their virulence”. Thesis will be submitted by the mid of May, 2013. 2000 – 2003 Microbiology, University Of Calcutta, India, Degree: Bachelor of Science (B.Sc.) (Honours). 2003 – 2005 Genetics, University Of Calcutta, India, Degree: Master of Science (M.Sc.). Experience 02/2007 – 03/2009 Senior Research Fellow (SRF), Plant Virus Laboratory and Biotechnology Unit, Central Research Institute for Jute and Allied Fibres (CRIJAF), Kolkata, India. 12/2005 – 01/2007 Junior Research Fellow, Manovikas Biomedical Research and Diagnostic Centre, Kolkata, India. 05/2004 – 07/2004 Summer Research Student, Immunotechnology Section (now Division of Molecular Medicine), Bose Institute, Kolkata, India. Technical skills Molecular biology: Genomic DNA extraction from plant tissue, human blood, bacteria and fungi, extraction of RNA from bacteria and fungi, DNA and RNA quantification, Polymerase Chain Reaction (PCR), reverse transcriptase PCR (RT PCR), Quantitative PCR (qPCR), bacterial transformation and cloning, gel electrophoresis (agarose and SDS PAGE), RAPD, RFLP and SNP polymorphism techniques, Southern DNA hybridisation, protein expression in bacteria, extraction and cloning of double stranded RNA (dsRNA) elements from fungi. Microbiology: Pure culture techniques for bacteria and fungi, staining and microscopy of bacteria and fungi, fungal anastomosis methods (Rhizoctonia solani). Plant pathology: Pathogenicity assessments in glasshouse and shadehouse trial, Harvesting trials, assessments of plant-disease parameter, data collection, manipulation and analyses. 1 Paper publications 1. Das, S., Roy, A., Ghosh, R., Paul, S., Acharyya, S., & Ghosh, S. K. (2008). Sequence variability and phylogenetic relationship of betasatellite isolates associated with yellow vein mosaic disease of mesta in India. Virus Genes. 37, 414-424. 2. Das, S., Ghosh, R., Paul, S., Roy, A., & Ghosh, S. K. (2008) Complete nucleotide sequence of a monopartite begomovirus associated with yellow vein mosaic disease of mesta from north India. Archives of Virology. 153, 1791-1796. 3. Dutta, S., Das, S., Guhathakurta, S., Sen, B., Sinha, S., Chatterjee, A., Ghosh, S., Ahmed, S., Ghosh, S., & Usha, R. (2007). Glutamate receptor 6 Gene (Glur6 or GRIK2) polymorphisms in the Indian population: a genetic association study on autism spectrum disorder. Cellular and Molecular Neurobiology. 27, 1035-1047. 4. Roy, A., Acharyya, S., Das, S., Ghosh, R., Paul, S., Srivastava, R. K., & Ghosh, S. K. (2009). Distribution, epidemiology and molecular variability of the begomovirus complexes associated with yellow vein mosaic disease of mesta in India. Virus Research. 141, 237-246. 5. Paul, S., Ghosh, R., Das, S., Palit, P., Acharyya, S., Das, A., Mir, J. I., Chaudhuri, S., Ghosh, S. K., & Roy, A. (2009). First report of Tomato leaf curl Joydebpur virus and associated betasatellite in kenaf (Hibiscus cannabinus) plants showing leaf curl symptoms from southern India. Plant Pathology. 58, 403. 6. Paul, S., Roy, A., Ghosh, R., Das, S., Chaudhuri, S., & Ghosh, S. K. (2008). Molecular characterization and sequence variability of betasatellites associated with leaf curl disease of kenaf (Hibiscus cannabinus L.) from different geographical locations of India. Acta Virologica. 52, 251-256. 7. Ghosh, R., Paul, S., Das, S., Palit, P., Acharyya, S., Das, A., Mir, J. I., Ghosh, S. K., & Roy, A. (2008). Molecular evidence for existence of a New World begomovirus associated with yellow mosaic disease of Corchorus capsularis in India. Australasian Plant Disease Notes. 3, 59-62. 8. Ghosh, R., Paul, S., Palit, P., Das, S., Das, A., Acharyya, S., Mir, J. I., Ghosh, S. K., & Roy, A. (2008). Molecular characterization and computational analysis of coat protein gene of an east Indian isolate of Bhendi yellow vein mosaic virus and associated DNA-beta satellite. Indian Journal of Virology. 19, 173-182. Submitted manuscript: 1. Das, S., Shah, F. A., Butler, R. C., Falloon, R. E., Stewart., A., & Pitman, A. R. The Anastomosis Group composition, genetic variability and pathogenicity of Rhizoctonia solani isolates associated with black scurf of potato in New Zealand. Communicated to the journal Plant Pathology. Abstract Publications 1. Das, S., Shah, F. A., Falloon, R. E., Butler, R. C., & Pitman, A. R. (2010). Characterisation of Rhizoctonia solani Anastomosis Group 2-1 from potato tubers in New Zealand. Proceedings of 6 th Australasian Soilborne Diseases Symposium. p 41. 2. Das, S., Paul, S., Ghosh, R., Palit, P., Acharyya, S., Das, A., Mir, J. I., Ghosh, S. K., & Roy, A. (2008). Occurrence of a Begomovirus and satellite DNA associated with yellow vein disease of Sida sp. from eastern India and its computational analyses. Indian Journal of Virology. 19, 119. 2 3. Das, S., Ghosh, R., Paul, S., Palit, P., Acharyya, S., Das, A., Mir, J. I., Ghosh, S. K., & Roy, A. (2007). Molecular variability of Begomovirus complex associated with yellow vein mosaic disease of mesta in Northern and Eastern India. National Symposium on Microbial Diversity and Plant Health. P 11. 4. Dutta, S., Das, S., Sinha, S., Chatterjee, A., Ahmed, S., Ghosh, S., Gangopadhyay, P. K., & Usha, R. (2007). An Association Study of Glutamate Receptor 6 gene with Autism in the Indian Population. The 3rd International Symposium on “Neurodegeneration and Neuroprotection” and Society for Neurochemistry (India) Meeting. 5. Ghosh, R., Paul, S., Das, S., Acharyya, S., Das, A., Palit, P., Mir, J. I., Ghosh, S. K., & Roy, A. (2008). First report of association of a satellite DNA from croton plant showing yellow vein Symptoms in Eastern India. Indian Journal of Virology. 19, 118. 6. Paul, S., Ghosh, R., Das, S., Acharyya, S., Palit, P., Das, A., Mir, J. I., Ghosh, S. K., & Roy, A. (2008). Detection of a Begomovirus associated with yellow vein disease of Eclipta prostrata in eastern India. Indian Journal of Virology. 19, 120. 7. Ghosh, R., Paul, S., Das, S., Palit, P., Acharyya, S., Mir, J. I., Roy, A., & Ghosh, S. K. (2007). Association of Begomovirus complex with yellow mosaic disease of jute in India. Proceedings of 10th Plant Virus Epidemiology Symposium. p 87. 8. Paul, S., Ghosh, R., Das, S., Palit, P., Acharyya, S., Mir, J. I., Chaudhary, S., Ghosh, S. K., & Roy, A. (2007). First sequence based evidence for occurrence of recombinant Begomovirus and a satellite β DNA associated with leaf curl disease of kenaf (Hibiscus cannabinus) in Northern India. Proceedings of 10th Plant Virus Epidemiology Symposium. p 118. 9. Ghosh, R., Paul, S., Das, S., Das, A., Acharyya, S., Ghosh, S. K., & Roy, A. (2008). Molecular detection of a New World bipartite begomovirus in jute crop. XVIII National Conference of India Virological Society. p 44. 10. Ghosh, R., Paul, S., Das, S., Das, A., Acharyya, S., Ghosh, S. K., & Roy, A. (2008). Detection and characterization of a New World begomovirus in jute (Corchorus capsularis) from India. A journey from Plant Physiology to plant biology. An international symposium to commemorate The 150 th birth anniversary of Sir J. C. Bose & Birth Centenary of Prof. S.M. Sirkar. p 82. 11. Acharyya, S., Paul, S., Ghosh, R., Das, S., Palit, P., Das, A., Mir, J. I., Ghosh, S. K., & Roy, A. (2008). Molecular evidence for natural occurrence of Bhendi yellow vein mosaic virus in Urena lobata from eastern India. Indian Journal of Virology. 19, 119. 12. Das, A., Paul, S., Ghosh, R., Das, S., Palit, P., Acharyya, S., Mir, J. I., Ghosh, S. K., & Roy, A. (2008). Association of a Begomovirus with yellow mosaic disease of Clerodendron sp. occurring in eastern India as revealed by In-silico analysis. Indian Journal of Virology. 19, 118. 13. Palit, P., Ghosh, R., Paul, S., Das, S., Das, A., Acharyya, S., Mir, J. I., Ghosh, S. K., & Roy, A. (2008). Characterization of coat protein gene of Bhendi yellow vein mosaic virus and associated satellite DNA from Eastern India. Indian Journal of Virology. 19, 119-120. 14. Roy, A., Ghosh, R., Paul, S., Das, S., Palit, P., Acharyya, S., Das, A., Mir, J. I., & Ghosh, S. K. (2008). Molecular evidence for existence of new emerging Begomoviruses from bast fibre crops in India. Indian Journal of Virology. 19, 76. 15. Roy, A., Ghosh, R., Paul, S., Das, S., Palit, P., Acharyya, S., Mir, J. I., & Ghosh, S. K. (2007). Characterization of a monopartite recombinant begomovirus and satellite DNA β associated with yellow vein mosaic disease of mesta crop in India. Proceedings of 10th Plant Virus Epidemiology Symposium. p 59. 3 16. Roy, A., Ghosh, R., Paul, S., Das, S., Palit, P., Acharyya, S., Das, A., Mir, J. I., & Ghosh, S. K. (2008). Newly emerging begomoviruses on bast fibre crops in India. International symposium on Jute and Allied Fibres Production, Utilization and Marketing. p 83. 17. Das, A., Ghosh, R., Paul, S., Das, S., Acharyya, S., Ghosh, S. K., & Roy, A. (2008). Sequenced based evidence for occurrence of Begomovirus and satellite DNA β associated with leaf deformity syndrome of Sunn-hemp in East India. A journey from Plant Physiology to plant biology. An international symposium to commemorate The 150 th birth anniversary of Sir J. C. Bose & Birth Centenary of Prof. S.M. Sirkar. p 70. Symposia and conferences 1. Bio-Protection Symposium, managing pests: the future of biocontrol. August 31st, 2011, Lincoln University, Christchurch, New Zealand (Poster presentation). 2. Horticulture New Zealand Conference. July 26-27, 2011, Rotorua, New Zealand (Poster presentation). 3. 6th Australasian soilborne diseases symposium. August 9-11, 2010, Queensland, Australia. (Oral presentation). 4. International symposium on “A Journey from Plant Physiology to Plant Biology” at the Bose Institute. November 24-28, 2008; Kolkata, India. 5. International symposium on Jute and Allied Fibres: production, Technology and Marketing. January 10-12, 2008; Kolkata, India. 6. International Symposium on Management of Coastal Ecosystem: Technological Advancement and Livelihood Security. Organized by: Indian Society of Coastal Agricultural Research. October 27-30, 2007; Kolkata, India. 7. 10th International Plant Virus Epidemiology Symposium on Controlling Epidemics of Emerging and Established Plant Virus Diseases-The Way Forward at International Crops Research Institute for the Semi-Arid Tropics (ICRISAT). October 15-19, 2007; Hyderabad, India. 8. 3rd International Symposium on “Neurodegeneration and Neuroprotection” and Society for Neurochemistry (India) Meeting at Indian Institute of Chemical Biology. January 8-9, 2007; Kolkata, India. 9. International conference on emerging and re-emerging viral diseases of the tropics and subtropics, December 11-14, 2007, New Delhi, India. (Poster presentation). 10. National symposium on Microbial diversity and plant health, November 29-30, 2007; West Bengal, India (Poster presentation). 11. National Seminar on “Classical Genetics and Modern Biotechnology” organized by Centre Of Advanced Study in Cell and Chromosome Research, University Of Calcutta. January 24-25, 2005; Kolkata, India. 4 Honors & Awards Awarded with the “Bio-Protection Research Centre Writing Scholarship” in February, 2013. Language skills Bengali (native language), Hindi (very good oral skills) and English (very good oral and written skills) Hobbies Cycling, reading, travelling, music and photography References Prof. Richard Falloon Position: Deputy Director Bio-Protection Research Centre Email: richard.falloon@plantandfood.co.nz Phone: +64 3 325 6400 / +64 3 325 3696 ext 8633 Fax: +64 3 325 2074 Location: Plant & Food Research, Lincoln Division: Executive Management Group Sustainable bio-protection (Doctorate supervisor) Prof. Alison Stewart Chief Science Officer Marrone Bio Innovations, Inc. astewart@marronebio.com (Associate doctorate supervisor) Dr. Andrew Pitman Position: Scientist Phone: +64 3 325 9520 Fax: +64 3 325 2074 Email: andrew.pitman@plantandfood.co.nz Location: Plant & Food research, Lincoln (Associate doctorate supervisor) Christchurch, 29th April, 2013 Subha Das 5