Table S3. Summary of the 11 microsatellite primers used for paternity analysis and quantification of
relatedness between assigned parents in two dipterocarp species Shorea xanthophylla and Parashorea
tomentella. Number of alleles (Na); observed heterozygosity (Hobs); expected heterozygosity (He);
paternity non-exclusion probability at each locus (N-PE) and total exclusion probability over all loci (PE)
given known mother. a Redesigned primers based on published primers. b Newly developed microsatellite primers. c
Published primers.
S.xanthophylla
(108 adults)
Locus
Dip01a
Dip02 a
Dip03 a
Dip04 a
Dip05 a
Pt05 b
SLD1c
Sx02 a
Sx10 a
mean
PE
Na
4
12
9
10
9
6
15
27
19
12
Size range (bp)
89-95
206-229
137-154
146-190
175-192
103-117
181-212
200-253
121-179
Hobs
0.062
0.716
0.48
0.295
0.621
0.688
0.839
0.795
0.897
0.599
He
0.089*
0.78
0.524
0.568*
0.694
0.694
0.809
0.876
0.889
0.658
N-PE
0.954
0.396
0.679
0.683
0.514
0.559
0.358
0.238
0.217
0.511
0.9991
P.tomentella
(93 adults)
Locus
Dip01a
Dip02 a
Dip03 a
Dip04 a
Dip05 a
Pt05 a
SLD1c
SLK06 c
SLC06 c
mean
PE
Na
6
10
7
6
7
10
7
5
12
8
Size range (bp)
102-116
212-240
134-146
158-172
178-198
97-119
189-237
144-156
202-224
Hobs
0.506
0.75
0.231
0.659
0.368
0.651
0.194
0.627
0.667
0.517
He
0.519
0.781
0.312*
0.741
0.428
0.785
0.368*
0.567
0.789
0.588
N-PE
0.7
0.425
0.839
0.501
0.752
0.419
0.792
0.726
0.391
0.616
0.9912
GeneBank
Accession
AJ582737
AJ616883.1
AJ616888.1
AY558717
AJ616885
FJ968736
DC651058
FJ968737
FJ968738
AJ582737
AJ616883.1
AJ616888.1
AY558717
AJ616885
FJ968736
DC651058
DC649188
DC650703
Table S3. Mating system statistics for progeny of Shorea xanthophylla and Parashorea tomentella
based upon 9 microsatellites loci. Number of progeny genotypes (N); multilocus outcrossing rate (tm );
single locus outcrossing rate (ts); , biparental inbreeding as defined by the difference between multilocus
and single locus outcrossing rates (tm - ts); Parental inbreeding coefficient. Values in parenthesese are
standard error (SE) based upon 100 bootstraps.
S.xanthophylla
N
456
P.tomentella
408
tm
0.996
(0.0097)
0.907
(0.0014)
ts
0.9
(0.0022)
0.854
(0.0008)
tm - ts
0.096
(0.0102)
0.053
(0.0007)
Fp
0.094
(0.0046)
-0.031
(0.0018)