Universiteit van die Vrystaat
University of the Free State
Boc314
Semestertoets 2 memo / Semester test 2 memo
Assessor: Dr B Visser
Moderator: Prof J Albertyn
Tyd / Time: 2 h
Vraag 1 / Question 1
1.1
Molecule that when excited by a laser
Gives of visible light that can be detected
1.2
All the DNA present within a plant cell that includes the nucleus
Mitochondrion
And chloroplast
1.3
Represents all the genes that are expressed
1.4
A gene sequence that starts with a start (ATG) codon
And ends with a stop codon (TGA, TAG, TAA)
1.5
When a particular codon used for an amino acid
Within the coding region of a gene
Differs from those used outside of the coding region
1.6
It when the genes on a
Chromosome of a sequenced genome
Occur in the same order to those
On the chromosome of a related plant
Punte / Marks: 65
[15]
(2)
(3)
(1)
(2)
(3)
(4)
Vraag 2 / Question 2
[12]
2.1
Denatures the RNA run on the gel
(1)
2.2
Blocks
The nylon membrane to prevent the probe from binding
2.3
Fixes the RNA
To the nylon membrane
2.4
Acts as the substrate
For luciferase
To produce light
2.5
Balances the pH during the separation of RNA on a denaturing agarose gel
2.6
Carries the current within the agarose gel
2.7
Acts as a cofactor
For Taq DNA Polymerase
Vraag 3 / Question 3
3.1
Destroy all
Forms of nucleotides
3.2
Converts PPi
To ATP
By binding it to Adenosine 5’-phosphate
3.3
Converts luciferin
To light
By using ATP
3.4
It is a RNA dependent (uses RNA as template)
DNA polymerase (to synthesize DNA)
3.5
It has the 5’-3’ polymerization activity
And the 3’-5’ exonuclease activity (proofreading activity)
But not the 5’-3’ exonuclease activity
(2)
(2)
(3)
(1)
(1)
(2)
[13]
(2)
(3)
(3)
(2)
(3)
Vraag 4 / Question 4
[8]
4.1
Base correctly indicated on carbon 1
Pentose sugar with double bond oxygen
Hydrogen on carbon 2
Hydrogen on carbon 3
Tri-phosphate on carbon 5 (½ mark if not completely given)
(5)
4.2
One is PCR based thus cycling dependent and the other not
One makes use of radio-actively labelled ddNTPs and the other one fluophore labelled ddNTPs
One is divided into four tubes each having a single ddNTP whereas the other has a single
reaction containing all four nucleotides
One is detected using an x-ray and the other using a computer and laser beam
Use taq pol instead of a normal DNA polymerase
(3)
Vraag 5 / Question 5
[6]
5.1
The genomes of eukaryotes are much larger
The intergenic regions are much larger
Eukaryotic genes have introns within them
(3)
5.2
While the deletion cassette physically disrupts the gene
Antisense technology leaves the gene intact
It is only the expression that is repressed
(3)
Vraag 6 / Question 6
[11]
6.1
Reference genes are genes that are expressed constitutively
Irrespective of treatment, age of tissue
Reference genes are included to confirm that equal amounts of RNA is used in the expression
experiment
It should thus produce comparable results between all the different samples
(4)
6.2
Extract total RNA
from wheat leaves
Add:
Buffer (½)
dNTPs (½)
primers
specific for gene X
reverse transcriptase (½)
Taq DNA polymerase (½)
After completion separate on an agarose gel
___________________________________
(7)