Page 1 of 2 BUGSS Chemical White List The following chemicals are permitted in the BUGSS facility as long as they are in their original labeled container, specifying the identity, concentration, hazard rating and storage conditions. A MSDS must accompany the chemical and will be placed in the BUGSS MSDS file. For chemicals not on this white list specific written permission is required from the BUGSS safety officer (safety@bugssonline.org) before the chemical can be allowed into the facility. = MSDS on File in Laboratory _________________________________________________________________________________________________________ Calcium Chloride Ammonium chloride Magnesium chloride Tryptone Yeast Extract Peptone Amino Acids L-proline Vitamins Agar Magnesium sulfate Gel-Red Brome phenol Blue Glycerol Triton X-100 Glucose Sucrose Tween 20 Galactose IGEPAL®CA-630 Ethanol (quantity limited) Arabinose Raffinose Agarose Isopropanol (quantity limited) Tris-HCL Tris Base Sodium acetate Sodium chloride BUGSS Chemical White List: Version 1.2 Effective 5_2_2014 DMSO (quantity limited) IPTG Ampicillin Kanamycin Chloramphenicol Tetracycline Lithium acetate Potassium Chloride Acetic Acid (quantity limited) HCL (quantity limited) NaOH (quantity limited) Lysozyme RNase A Restriction and other DNA modifying enzymes Zymolyase Calf Thymus DNA Anti-mouse IgG BCIP®/NBT solution EDTA Murashige & Skoog Basal medium Page 2 of 2 Murashige & Skoog Basal salts with minimal organics Sodium dodecyl sulfate Tryptic soy broth D-mannitol 10% FeCl3 Instant Ocean PEG-3350 Sephadex G-50 Potassium phosphate monobasic Potassium phosphate dibasic Yeast nitrogen base w/ ammonium sulfate w/o amino acids Bromophenol blue ZnCl2 1M solution Kinetin 1mg/ml Alpha-Naphthaleneacetic acid 1mg/ml ____Nothing Follows_____ Simply Blue Safe Stain Tris-Glycine running buffer 10X Gram stain kit w/ grams iodine, crystal violet, saffron red Lactose _________________________________________________________________________________________________________ Change History: Version & Effective Date Chemical white list v1.1 Effective 3/1/2013 Chemical white list v1.2 Effective 5/2/2014 BUGSS Chemical White List: Version 1.2 Effective 5_2_2014 Changes Original Document Additions to white list and chcekmarks for on-file MSDS