CLASS SET
Chapter 6 Review Guide: Identifying a Potential Biotechnology Product—
Directions: Please answer the following questions ON YOUR OWN PAPER and IN COMPLETE
SENTENCES.
1. What does CPDP stand for?
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3.
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Describe the steps of a CPDP.
What is another name for “testing” products?
What is the difference between an activity assay and a concentration assay?
Name two examples of a concentration assay?
What is an IND application?
What is the difference between a pharmacokinetic (PK) assay and a pharmacodynamics (PD)
assay?
8. Define potency assay.
9. Define toxicology assay.
10. Define stability assay.
11. What does ELISA stand for?
12. What is the function or purpose of the ELISA assay?
13. Draw and explain the DIRECT ELISA assay.
14. Give an example of when this assay would be used. (Explain)
15. Draw and explain the INDIRECT ELISA assay.
16. Give a detailed example of when this assay would be used. (Explain)
17. What is the function (definition) of a western blot assay?
18. What are the two kinds of membranes that are used for this assay?
19. Draw a picture of the “sandwich” for the western blot protein transfer.
20. What does SDS-PAGE stand for?
21. What kinds of samples are run on this type of gel? What kind of gel box is used?
22. What is the name of the detergent that is used in SDS-PAGE gels?
23. Explain in detail WHY a detergent must be used?
24. What is the end result of running a SDS-PAGE gel? Name two things that can be done after
the gel run is complete?
25. Proteins run on a SDS-PAGE gel separate based on SIZE. (true or false)
26. Explain the steps of a western blot assay.
27. What is the end result of a western blot assay?
28. Look in your glossary. When is a Northern blot used? When is a Southern blot used?
29. Define herbal remedies.
30. Define antimicrobial.
31. Define antiseptic.
32. What is a nutraceutical?
33. Why are nutraceuticals controversial?
34. Look at the herbal therapy list on page 180, What herbal remedies are used to treat
infection; dry skin; common cold; and acne.
35. What did you find the most interesting in this chapter? Be specific/explain