Detection of Gene Mutations Techniques Chart

MLAB 2321 Molecular Diagnostics for Clinical Laboratory Science
Unit11 Detection of Gene Mutations
Techniques Chart
Name _________________________________
Points: _______/44
Please use this chart to assist you in categorizing the different techniques, stating whether or not the technique is currently used in the clinical
laboratory setting and any other special notes you might wish to add. You may use this “cheat sheet” for Exam 3.
1. Each item is worth 3 points each: 0.5 for Technique and Clinical Lab, 2 points for description.
2. Column 1, “Technique”, place an “” in the appropriate sub-column of the technique used: H=hybridization, S=sequencing , C=Cleavage
3. Column 2, “Clin Lab?” place a “Y” if this is used in the clinical laboratory, “N” for no.
4. Column 3, Briefly describe the technique. Provide enough information to assist you in selecting the correct answers on the exam.
1. Allele Specific Oligomer (ASO) hybridization
2. Allelic discrimination
3. Bead Array
4. Denaturing Gradient Gel Electrophoresis (DGGE)
5. Dideoxy DNA Fingerprinting
6. Heteroduplex analysis
7. Melt Curve Analysis (MCA)
8. Multiplex inversion probe assay
9. Sequence Specific PCR (SSP-PCR)
10. Single strand conformation polymorphism (SCCP)
11. Dye Termination Incorporation
12. Protein Truncation Test
13. Restriction Fragment Length Polymorphism
14. Heteroduplex Analysis with Single-Strand Specific Nucleases
15. Base Excision Sequence Scanning
16. Nonisotopic RNase Cleavage Assay
17. Invader Assay
18. Chemical Cleavage
Clin Lab?