Online Resource 2
The full set of Kruskal-Wallis tests to show significant and non-significant differences in
values of the three measures of genetic diversity and population size categories (a), IUCN
Red List categories (b), and studies using all three control region domains (c). Unequal
variance t-tests and supporting Wilcoxon rank sum tests to show significant and nonsignificant differences in mean and median values of genetic diversity between island,
mainland and island endemic species (d).
(a)
sizes: <100 & 100-500
0.8
0.6
present at bottleneck
0.4
Significant difference
Haplotype Diversity
1.0
Haplotype Diversity:
(H3=15.61, p<0.01**)
<100
101-500
501-1000
Non-bottleneck
n= 9
n= 4
n= 3
n= 55
<100
101-500
501-1000
Non-bottleneck
n= 9
n= 4
n= 3
n= 55
Bottleneck Size
size: <100
(H3=19.61,
p<0.001***)
5
4
3
2
present at bottleneck
1
Significant difference
Standard Number of Haplotypes
Haplotypes:
6
Standard Number of
Bottleneck Size
0.012
0.008
0.004
0.000
Nucleotide Diversity
Nucleotide Diversity
<100
101-500
501-1000
Non-bottleneck
n= 9
n= 4
n= 3
n= 55
Bottleneck Size
(b)
Haplotype Diversity
0.6
Haplotype Diversity
0.2
0.4
categories
0.8
1.0
and IUCN
Critically-Endangered
Endangered
n= 3
n= 14
Critically-Endangered
Endangered
n= 3
n= 14
Vulnerable
Near-Threatened
Least-Concern
n= 11
n= 14
n= 36
Vulnerable
Near-Threatened
Least-Concern
n= 11
n= 14
n= 36
IUCN Category
n= 3
Standard Number of
6
5
4
3
2
1
IUCN categories
Standard Number of Haplotypes
Haplotypes and
IUCN Category
n= 3
Nucleotide Diversity
0.008
Nucleotide Diversity
0.000
0.004
categories
0.012
and IUCN
Critically-Endangered
Endangered
n= 3
n= 14
Vulnerable
Near-Threatened
Least-Concern
n= 11
n= 14
n= 36
IUCN Category
(c)
1.0
Haplotype Diversity:
0.8
0.7
Haplotype Diversity
0.6
0.5
domains.
0.9
Control Region all
<100
n= 6
501-1000
Non-bottleneck
n= 2
n= 14
Bottleneck Size
n= 3
5
4
3
domains.
2
Control Region all
1
Haplotypes:
Standard Number of Haplotypes
Standard Number of
Significant difference
<100
present at bottleneck
n= 6
501-1000
Non-bottleneck
n= 2
n= 14
501-1000
Non-bottleneck
n= 2
n= 14
Bottleneck Size
size: <100
(H2=9.02, p<0.05*)
0.008
0.000
domains
Nucleotide Diversity
Control Region all
0.004
Diversity:
0.012
Nucleotide
<100
n= 6
Bottleneck Size
(d)
Haplotype diversity
Standard Number of
haplotypes
Nucleotide diversity
0.012
0.008
0.004
Nucleotide Diversity
6
5
4
3
2
Mainland
Island
0.000
Island
1
Standard Number of Haplotypes
0.8
0.6
0.2
(n = 11,68)
Haplotype Diversity
species
0.4
mainland
1.0
Island or
Mainland
Island
Mainland
Endemic
Non-Endemic
p<0.05 & W=237.5, p<0.05
t15.8t(15.759)=-2.179,
=-2.179, p<0.05;
W=237.5,p<0.05
Endemic
Non-Endemic
0.010
0.002
0.006
Nucleotide Diversity
3.0
2.5
2.0
1.5
1.0
0.6
0.7
0.8
Standard Number of Haplotypes
(n =6,6)
Haplotype Diversity
endemics
0.5
non-
0.4
endemics or
0.5
0.9
Island
Endemic
Non-Endemic
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