Online Resource 3
Dynamics of the endosymbiont Rickettsia in an insect pest
Microbial Ecology
Bodil N. Cass1, Rachel Yallouz2, Elizabeth C. Bondy3, Netta Mozes-Daube2, A. Rami Horowitz4,
Suzanne E. Kelly3, Einat Zchori-Fein2, Martha S. Hunter3§
1
Graduate Interdisciplinary Program in Entomology and Insect Science, 410 Forbes, University of
Arizona, University of Arizona, Tucson, AZ 85721 USA
2
Department of Entomology, Newe Ya’ar Research Center, P.O. Box 1021, Ramat Yishay, Israel
3
Department of Entomology, 410 Forbes, University of Arizona, Tucson, AZ 85721 USA
4
Department of Entomology, ARO, Gilat Research Center Mobile Post Negev, 85280, and Katif
Research Center, Sedot Negev, Israel
§
Corresponding author, mhunter@ag.arizona.edu
Fig. S1 Phylogenetic analysis of citrate synthase (gltA) gene sequences using maximum likelihood
places the Israel field, Israel laboratory, USA field and USA laboratory sequences in the same clade
along with other Rickettsia from B. tabaci. Bootstrap probability values ≥75 are indicated above
branches, whereas posterior probabilities ≥0.95 of the topologically identical BI consensus tree are
below branches. Sequences from this study are indicated in bold. Sequences from GenBank show
accession numbers in parentheses. Rickettsia groups are indicated in upper case text. Rickettsia
endosymbionts without species names are indicated with (s) and the host name.
Fig. S2 Phylogenetic analysis of rpmE-tRNAfMet intergenic spacer sequences using maximum
likelihood places the Israel field, Israel laboratory, USA field and USA laboratory sequences in the same
clade along with other Rickettsia from B. tabaci. Bootstrap probability values ≥75 are indicated above
branches, whereas posterior probabilities ≥0.95 of the topologically identical BI consensus tree are
below branches. Sequences from this study are indicated in bold. Sequences from GenBank show
accession numbers in parentheses. Whilst this tree shows clearly that this fast-evolving sequence is
invariant among USA and Israel Rickettsia, there was no statistical support for the other clades in the
tree, indicating that this region that is very useful to discriminate bacterial species and strains, is likely
not suitable for phylogenetic reconstruction, perhaps as it is evolving too quickly.