ISLH 2014 Welcome Program Pre-Meeting Workshops Registration Abstract Submission Awards Travel Continuing Education Conference Venue Hotel Banquet Poster Presentation Luncheon Workshops Exhibit Information Exhibitor Directory Exhibitor Checklist Wed - May 14 Thu - May 15 Fri - May 16 Sat - May 17 THURSDAY, MAY 15, 2014 CONFERENCE DAY 1 9:00 - 12:00 PM PLENARY SESSION OPENING PLENARY SESSION Chair/Organizer: Charles Eby, Catherine Hayward, Maria Proytcheva, Piet Meijer World Forum Theater 9:10 Can MRD Predict the Prognosis of CLL Paolo Ghia. Universita Vita-Salute San Raffaele, Milano, Italy 9:40 Diagnosis and Treatment of DIC Marcel Levi. Academic Medical Centre, Amsterdam, Netherlands 10:10Getting Into The Flow: Red Cells Go on a Roll Annie Viallat. Aix Marseille Université, Aix-en-Provence, France 10:40Coffee Break. 11:15Berend Houwen Lecture: Classification and Monitoring of Hematopoietic Neoplasia in the Genetic Era Brent Wood. University of Washington, Seattle, WA, United States 12:15 - 1:45 PM WORKSHOP ABBOTT LUNCHEON WORKSHOP Yangtze 1 & 2 Engineering the Future: Importance of Human Factors in Platform Development Richard KENDALL . Abbott Laboratories (Hematology), California, USA Diamonds in the Rough - Mining Big Data for Novel Biomarkers Wouter W van SOLINGE. University Medical Center, Utrecht, The Netherlands CELLAVISION LUNCHEON WORKSHOP Onyx Get Your RBC’s in Shape; Using a New and Innovative Digital Cell Morphology Software From CellaVision. 2:00 - 3:30 PM CONCURRENT SESSION CONCURRENT 1: ADVANCES IN MOLECULAR DIAGNOSTICS IN HEMATOLOGIC DISORDERS - LESSONS LEARNED Chair/Organizer: Albert Huisman, John Frater World Forum Theater 2:00 Molecular Diagnosis of Porphyrias Robert Desnick. Mount Sinai School of Medicine, New York, NY, United States 2:30 Molecular Diagnosis of AML/Next Generation Sequencing David Grimwade. King's College London, London, United Kingdom 3:00 Molecular Pathogenesis of AML - Epigenetic Considerations Ruud Delwel. Institute of Hematology, Erasmus University, Rotterdam, Netherlands CONCURRENT 2: DIRECT ORAL ANTICOAGULANTS Chair/Organizer: Charles Eby, Piet Meijer Mississippi 2:00 Clinical Aspects of the Treatment With DOACs Trevor Baglin. Addenbrooke's Hospital, Cambridge, United Kingdom 2:30 Laboratory Testing for DOACs and Test Interferences Helen Mani. University Hospital, Frankfurt, Germany 3:00 Clinical Cases and Laboratory Testing DOACs Charles Eby. Washington University, St. Louis, MO, United States CONCURRENT 3: UPDATES IN POC HEMATOLOGY Chair/Organizer: Carol Briggs, Linda Sandhaus Amazon 2:00 New CBC POC Devices Carol D'Souza. Westminster University, London, United Kingdom 2:30 Ensuring Patient Safety Through Innovative Training for Point Of Care Simon Kimber. School of Healthcare Science, Manchester Metropolitan University United Kingdom 3:00 Point of Care Testing at the Community Level in Eastern Africa Jane Carter. African Medical and Research Foundation, Nairobi, Kenya 3:30 - 4:00 PM BREAK BREAK Atlantic 4:00 - 4:45 PM PLENARY SESSION SPECIAL PLENARY Chair/Organizer: Albert Huisman World Forum Theater 4:00 Stem Cells in Hematologic Disease Hans Clevers. Hubrecht Institute, University Medical Center Utrecht, Utrecht, Netherlands & Royal Netherlands Academy of Sciences, Amsterdam, Netherlands 4:45 - 6:30 PM POSTER EXHIBITOR'S RECEPTION/ POSTER SESSION I Atlantic Comparison of nucleated red blood cell counts on XE5000 and XN-1000 against automated microscopy and flow cytometry.. HOWELL-JOLLY BODY-LIKE INCLUSIONS IN PERIPHERAL BLOOD NEUTROPHIL IMAGES IN IMMUNOSUPPRESSED PATIENTS Valentín Ortiz, Laura Bigorra, Rodrigo Santacruz, Anna Merino. Hemotherapy-Hemostasis Department. CDB. Hospital Clinic., Barcelona Spain HEMOGLOBIN AND CELL BLOOD COUNTS: PROGNOSTIC VALUE IN CHRONIC LYMPHOCYTIC LEUKEMIA PATIENTS AT DIAGNOSIS Rodrigo Santacruz1, Julio Delgado2, Anna Merino1. 1Hemotherapy-Hemostasis Department. CDB. Hospital Clinic., Barcelona Spain, 2Clinical Hematology Service. ICMHO. Hospital Clínic, Barcelona Spain Molecular characterization of B-thalassemia in Egypt ahmed abdelneam1, Khalda Said2, Mona Fayez1, Ghada Elkamah3. 1Molecular Genetic and Enzymology department ,Human Genetic and Genome Research Division, National Research Center, cairo, Egypt, 2Molecular Genetic and Medicine department ,Human Genetic and Genome Research Division, National Research Center, Cairo, Egypt, 3Clinical Genetic department ,Human Genetic and Genome Research Division, National Research Center, Cairo, Egypt Clinical Significance of Co-expression of Aberrant Antigens in Acute Leukemia: a retrospective cohort study in Makah Al Mukaramah. NAHLA ABDULATEEF1,2, MANAR ISMAIL2,3, HANADI ALJEDANI4.1Hematopathology Associate Consultant in laboratory and blood bank department, King Abdullah Medical city, Makkah , Saudi Arabia, 2Associate professor in clinical pathology department, National Cancer Institute, Cairo University , Cairo, Egypt, 3Associate professor in laboratory medicine department, faculty of applied medical science, Um Al Qura University, Makkah, Saudi Arabia, 4Assistant consultant of Hematopathology and blood bank department, King Abdullah Medical city, Makkah, Saudi Arabia Sensitivity of Individual and Mini-Pool Nucleic Acid Testing Assessed By Dilution of Hepatitis B NAT Yield Samples nitin agarwal, kabita chatterjee. All India Institute of Medical Sciences, New Delhi, India HAEMATOLOGICAL PROFILE OF HOSPITALIZED PATIENTS INFECTED BY WEST NILE VIRUS Athanasia Agorasti, Agapi Kotanidou, Eutychia Nikolakopoulou. General Hospital of Xanthi, Xanthi, Greece Importance of Educational Activities in Reducing Nonconforming Laboratory Samples as a Quality Initiative Hamed Al Ghaithi, Anil Pathare, Rodrigo Vilacirasis, Salam Alkindi . Sultan Qaboos University Hospital, Muscat, Oman Rapid identification of two common δ-globin mutations: Hb A2' and Hb A2-Yialousa using high resolution melting analysis Shoaib AL ZADJALI1, Salam Alkindi2, Arwa Z. Al Riyami1, Anil Pathare1, Mohammed AL Rawahi2, Shahina Daar2. 1Sultan Qaboos University Hospital, Muscat, Oman, 2Sultan Qaboos University, Muscat, Oman Evaluation of coagulation status in north Indian healthy volunteers using thromboelastography and conventional methods Venencia Albert1, Arulselvi Subramanian1, Renu Saxena2, Deepak Agrawal3, Ravindra Mohan Pandey4. 1Department of Laboratory Medicine, Jai Prakash Narayan Apex Trauma Centre, AIIMS, New Delhi, India, 2Department of Haematology, AIIMS, New Delhi, India, 3Department of Neurosurgery, Jai Prakash Narayan Apex Trauma Centre, AIIMS, New Delhi, India, 4Department of Biostatistics, AIIMS, New Delhi, India A NEW STRATEGY FOR AUTOMATIC IDENTIFICATION OF ATYPICAL LYMPHOID CELLS FROM PERIPHERAL BLOOD CELLS IMAGES Santiago Alférez1, Anna Merino2, Laura Bigorra2, Luis E Mujica1, Magda Ruiz1, Jose Rodellar1. 1Universitat Politècnica de Catalunya, Barcelona Spain, 2Hemotherapy-Hemostasis Department. CDB. Hospital Clínic, Barcelona Spain Immediate fixation of treated blood samples enables flow cytometric measurement of platelet aggregation and platelet-leucocyte conjugate formation in small volumes of blood. Mohammad Algahtani, Natalia Dovlatova, Jane May, Stan Heptinstall , Sue Fox.University Of Nottingham , Nottingham , United Kingdom Non classical monocytes analysis in B-cell chronic disorders using multiparametric flow cytometry and HematoFlowTM. Kaoutar Allou1, Marie Christine Béné2, Jean Philippe Vial1, Francis Lacombe1. 1CHU Bordeaux, BORDEAUX, France, 2CHU Nantes, Nantes, France COMPARATIVE ANALYSIS OF HAEMOGLOBIN GENOTYPES AND RED CELL INDICES AMONG APPARENTLY HEALTHY BLOOD DONORS GLORIA AMEGATCHER1, RABI MUSA2, RANSFORD KYEREMEH2. 1KNUST, ACCRA, Ghana, 2UNIVERSITY OF GHANA, ACCRA Ghana, 3UNIVERSITY OF GHANA, ACCRA Ghana epidemiological,clinical and biological characteristics of B-thalassemia in northwest of Algeria moueden amine1, benlaledj driss1, beras khalida1, homane wassila1, moulasserdoun khedidja1, rahal chakib1, seghier fatima1. 1the hemobiology service CHUO, Oran, Algeria, 2the hemobiology service CHUO, Oran, Algeria, 3the hemobiology service CHUO, Oran, Algeria, 4the hemobiology service CHUO, Oran, Algeria, 5the hemobiology service CHUO, Oran, Algeria, 6the hemobiology service CHUO, Oran, Algeria, 7the hemobiology service CHUO, Oran, Algeria Diagnostic performance of the variant lymphocyte by Beckman Coulter LH780 hematology analyzer in hemorrhagic fever patients. Yupin Aniwatangkoora1, Supattra Mungkalachaiya2, Surintorn Sornsriya3, Nattaya Sae- ung1, Yossombat Changtrakul4. 1Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand, 2Taksin Hospital, Medical Service Department, Bangkok, Thailand, 3Somdet Phrayupharacha Dansai Hospital, Loei, Thailand, 4Srinagarind Hospital, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand Impact of Wilms tumor 1 (WT1)gene mutations inpatient with cytogenetically normal acute myeloid leukemia .Salah Aref, Solafa El Sharawy, Mohamed Sabry, Emaad Azmy, Tawfik Al Khodary.Mansoura University, Mansoura, Egypt Detection of Minimal Residual Disease by Flow Cytometry in Acute Leukemia. A Challenge for Standardization and Interpretation. NYDIA STRACHMAN BACAL1,2, LAIZ CAMEIRÃO BENTO1, ANA CAROLINA APELLE BORTOLUCCI1, SONIA TSUKASA NOZAWA1, DANIELA SCHIMIDELL1, RODOLFO PATUSSI CORREIA1. 1ALBERT EINSTEIN HOSPITAL, SAO PAULO, Brazil, 2CENTRO DE HEMATOLOGIA DE SÃO PAULO, SÃO PAULO, Brazil IKZF1 (IKAROS) deletions as a marker of minimal residual disease for patients with acute leukemia Ildar Barkhatov, Maria Zagrivnaja, Ludmila Zubarovskaya, Boris Afanasyev. Pavlov First State Medical University, St.Petersburg, Russia The Ratio Absolute Reticulocytes Count/ Immature Reticulocyte Fraction, a Very Useful Tool in the Screening of Hereditary Spherocytosis Rogerio Barreira, Patricia Ferreira, Joana Rita Pinheiro, Ana Teresa Simões, Ana Cristina Oliveira, Salvado Ramon, Jose Carlos Almeida, Maria Leticia Ribeiro. Centro Hospitalar e Universitário de CoimbraCHUC, Coimbra, Portugal Blood circulating atypical lymphocytes detection and enumeration using the Cytodiff©monoclonal antibodies panel on HematoFlow®platform. Ingrid Beukinga, Jonathan Brauner, Hazar Lahlou, Dominique Penninck, Zaïna Kassengera, Olivier Pradier. ULB-Hôpital Erasme, Brussels, Belgium SLIDE REVIEW CRITERIA ON THE SYSMEX XN1000 IN A PRIVATE LABORATORY IN MUMBAI, INDIA MILIND BHIDE, HARSHALA KULKARNI, MADHUKAR BHIDE, DEEPALI SURYAVANSHI. BHIDE LABORATORY SERVICES, MUMBAI, India Laboratory reporting of peripheral blood counts in Italy and Evidence Based Medicine Bruno Biasioli, Caterina Maconi, Piero Cappelletti, Anna Maria Cenci, Valentino Miconi, Paolo Doretto, Barbara Casolari, Cristina Feruglio. Hematology Working Group (GdS-E) of Italian Society of Laboratory Medicine (SIMeL), Castelfranco Veneto, Italy Alterations platelet function in gestational diabetes Volha Bichan1, Zoya Zabarovskaya2. 1Belarusian State University, Minsk, Belarus, 2Belarusian State Medical University, Minsk, Belarus Study of few cytokines genes polymorphism and evolution after HSCT DENISA BRATU1,2, ILEANA CONSTANTINESCU1,2, ANA MOISE1,2. 1CLINICAL INSTITUTE FUNDENI, BUCHAREST, Rumania, 2UMF,,CAROL DAVILLA,,, BUCHAREST, Rumania Blasts count with HematoFlow platform: 3 years’ experience in a middle-sized academic hospital. Jonathan Brauner, Ingrid Beukinga, Massimo Camaiani, Hazar Lahlou, Zaïna Kassengera, Olivier Pradier. Laboratory of Hematology, ULB-Hôpital Erasme, Brussels, Belgium Is laboratory validation of peripheral blood cell counts in Italy evidence-based? Piero Cappelletti, Bruno Biasioli, Valentino Miconi, Anna Maria Cenci, Mauro Buttarello, Elisa Piva, Fabrizio Papa, Marco Moretti. Hematology Working Group (GdS-E) of Italian Society of Laboratory Medicine (SIMeL), Castelfranco Veneto, Italy Validation of the cutoff values of the mean corpuscular volume and mean corpuscular hemoglobin on four automated analyzers for thalassemia screening Chaninthorn Chaitripop1, Kanokwan Sanchaisuriya2, Sakoun Inthavong3, Supan Fucharoen2, Goonnapa Fucharoen2, Yossombut Changtrakul4, Pattara Sanchaisuriya5. 1Medical Science Program, Graduate School, Khon Kaen University, Khon Kaen, Thailand, 2Centre for Research and Development of Medical Diagnostic Laboratories (CMDL), Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand, 3Bureau of Nutrition, Ministry of Health, Vientiane, Laos, 4Clinical Microscopy unit, Srinagarind Hospital, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand, 5Department of Nutrition Faculty of Public Health, Khon Kaen University, Khon Kaen, Thailand Concomitant monoclonal gammopathy of unknown significance (MGUS) in newly diagnosed myelodysplastic syndrome/acute myeloid leukemia (MDS/AML) Mingyi Chen1, Hanh La2, Mehrdad Abedi 2. 1Depatment of Pathology and Laboratory Medicine, UC Davis Medical Center, Sacramento, CA, United States, 2Division of Hematology, UC Davis Medical Center, Sacramento, CA, United States The lupus anticoagulant results from 1100 patients attending a high-risk pregnancy clinic Hee-Jung Chung1, Sun-Hee Kim1, Kyung-Sook Kang1, Hyun-Sook Kim1, Sung-Hee Lee2, So-Young Kim3. 1Cheil General Hospital & Women's Healthc, Seoul, South Korea, 2Hanmaeum Hospital, Jeju, South Korea, 3St. Paul's Hospital, The Catholic University of Korea, Seoul, South Korea The Hematology Laboratory in blood doping (BD): 2014 update on the Athlete Biological Passport (ABP) Giuseppe d'ONOFRIO1, Gina ZINI1, Neil ROBINSON2, Yorck Olaf SCHUMACHER3.1Catholic University, Rome, Italy, 2Swiss Laboratory for Doping Analyses, Lausanne, Switzerland, 3Aspetar Hospital, Doha, Qatar Polymorphisms in the ABO blood group gene in West Algerian population Tahria Deba1, Leila Adda Neggaz2, Asmahan Bekada3, Naima Meroufel2, Fatima Seghier1, Sonia Mediene Benchekor3, Soraya Benhamamouch3. 1Faculté de Médecine, Université d’Oran,, Oran, Algeria, 2Département de Génétique Moléculaire Appliquée, Faculté des Sciences, Université des Sciences et de Technologie Mohamed Boudiaf, Oran, , Oran, Algeria, 3Département de Biotechnologie, Faculté des Sciences de la Nature et de la Vie, Université d’Oran, Oran, Algeria, 4Département de Génétique Moléculaire Appliquée, Faculté des Sciences, Université des Sciences et de Technologie Mohamed Boudiaf, Oran, Oran, Algeria, 5Faculté de Médecine, Université d’Oran, Oran, Algeria, 6Département de Biotechnologie, Faculté des Sciences de la Nature et de la Vie, Université d’Oran, Oran, Algeria, 7Département de Biotechnologie, Faculté des Sciences de la Nature et de la Vie, Université d’Oran, Oran, Algeria Evaluation of bone marrow involvement in systemic mastocytosis by high-sensitive flow cytometric immunophenotyping. Elke Del Biondo, Peter Vandenberghe, Barbara Dewaele, Anne-Marie Kochuyt, Nancy Boeckx. UZ Leuven, Leuven, Belgium Comparison between N-Latex and Freelite assays for measuring free light chains in a clinical hematology laboratory. Yael Deutsher-Chertkow1, Efrat Marsh1, Eti Shem-Tov2, Ninette Amariglio 2, Clara Henig3, Noa Lavi4, Galit Sarig1. 1Hematology Laboratory, Rambam Health Care Campus, Haifa, Israel, 2Hematology Laboratory, Chaim Sheba Medical Center, Tel Hashomer, Israel, 3Haifa and Western Galilee Laboratory, Clalit Health Services, Nesher, Israel, 4Department of Hematology and Bone Marrow Transplantation, Rambam Health Care Campus, Haifa, Israel EFFECT OF BLOOD STORAGE ON OSMOTIC FRAGILITY Diweni Dick, Teddy Adias, Andrew Etima, Victor Ideede, Ayibatonye Orutugu, Idaye Reuben, Mercy Igoni, Christian Atiegha. Bayelsa State College of Health Tech., Yenagoa, Nigeria Case Study : Beta Thalassaemia Major with an underlying Apolipoprotein A5 mutation. Marco DiGirolamo, George Hero. Women's and Children's Hospital, North Adelaide, Australia Fine needle aspiration as a diagnostic technique in Bcell Non-Hodgkin lymphoma: the usefulness of combining cytomorphology with flow cytometric immunophenotyping and FISH analysis Hanaa Donia, Maged Mikhail, Nahla Farahat, Ashraf Elghandour, Amani Sorour.Faculty of Medicine, Alexandria, Egypt DEVELOPING A FLOW CYTOMETRIC METHOD FOR DETERMINING THE MUTATIONAL STATUS OF NUCLEOPHOSMIN (NPM1) IN ACUTE MYELOID LEUKAEMIA (AML) Louis Du Pisani, Karen Shires. National Health Laboratory Service, Cape Town, South Africa COAGULATION STATE IN DIABETIC RETINOPATHY IN TYPE 2 DIABETIC SUDANESE PATIENTS Manal Eltahir1, Maria Satti2. 1University of science& technology, Kharoum, Sudan, 2university of Khartoum, Khartoum, Sudan What is the Aetiology of Megakaryocytic Hyperplasia in Myeloproliferative Neoplasms? Wendy Erber1,2, Kathy Heel1, Ayesha Arshad1, Jacques Malherbe1, Katie Meehan1, Sara Hall1,2. 1University of Western Australia, Nedlands W.A., Australia, 2PathWest , Nedlands W.A. Australia UF-1000i: validation of the body fluid mode for counting cells in body fluids Chérina Fleming1, Rob Brouwer1, Ardriaan van Alphen2, Jan Lindemans1, Robert de Jonge1. 1Erasmus MC, University Medical Center, Rotterdam, Netherlands, 2Maasstad Hospital, Rotterdam, Netherlands Differentiation of Hb E-dbo-thalassemia and Hb E-bothalassemia by Hb-analysis using capillary electrophoresis Supan Fucharoen1, Nattaphol Prakobkaew1,2, Goonnapa Fucharoen1. 1Khon Kaen University, Faculty of Associated Medical Sciences, Centre For Research And Development Of Medical Laboratories, Muang, Thailand, 2Khon Kaen University, Graduate School, Muang, Thailand Nuclear Telomere Architecture as a Biomarker of Myelodysplastic Syndromes and Acute Myeloid Leukemias Macoura Gadji1,2, Prerana Rodrigues1, Sabine Mai1. 1Cheikh Anta Diop University of Dakar (UCAD) / National Centre of Blood Transfusion (CNTS) of Senegal, Dakar, Senegal, 2Manitoba Institute of Cell Biology (MICB)/CancerCare Manitoba (CCMB)/ University of Manitoba, Winnipeg, MB, Canada DETECTION OF MALARIAL PARASITE USING VCS TECHNOLOGY OF BECKMANN COULTER KOMAL GALANI1, SHASHIKANT MAHADIK2, VIJAYA GADAGE3, NILESH DESHPANDE4, PG SUBRAMANIAN5, SUMEET GUJRAL6. 1TATA MEMORIAL HOSPITAL, MUMBAI, India, 2TATA MEMORIAL HOSPITAL, MUMBAI, India, 3TATA MEMORIAL HOSPITAL, MUMBAI, India, 4TATA MEMORIAL HOSPITAL, MUMBAI, India, 5TATA MEMORIAL HOSPITAL, MUMBAI, India, 6TATA MEMORIAL HOSPITAL, MUMBAI, India Validation of the Cytognos Small Sample Tube (SST) for Flow Cytometric Evaluation of Paucicellular Clinical Specimens Peter Gambell1, Vuong Nguyen1, David Westerman1,2. 1Peter Maccallum Cancer Centre, East Melbourne, Australia, 2University of Melbourne, Parkville, Australia ABT-737 induces a distinct phenotype of apoptotic platelets lacking pro-inflammatory potentials Mehran Ghasemzadeh1, Ehteramolsadat Hosseini1, Shaun Jackson2. 1Blood Transfusion Research Center, High Institute for Education and Research in Transfusion Medicine, Tehran, Iran, 2Australian Centre for Blood Diseases, Monash University , Melbourne, Australia The Role of High von Willebrand Factor and Low ADAMTS13 Antigens Levels in the Risk of Venous Thromboembolism Christiane Gouvea, Sandra Matsuda, Rodrigo Vaez, Patricia Pinheiro, Maria Aparecida Noguti, Dayse Lourenço, Vania Morelli. Federal University of São Paulo, São Paulo, Brazil BRAF V600E : Single Mutation key to Hairy Cell Leukaemia Standarization of Allele specific PCR for detection of BRAF V600E in a Tertiary Care Haematology Center in India Dr.Monali Gupta, Chanchal Das, Sambhunath Banerjee, Subhajit Brahma, Saurav Sharma Choudhary, Dr.Saurabh Bhave, Dr.Anupam Chakrapani, Dr.Mayur Parihar, Dr.D.K. Mishra, Dr.Reena Nair, Dr.Mammen Chandy. Tata Medical Centre, Kolkata, India The Spanish web-based external quality assessment scheme in cytogenetic of haematological malignancies: Five years experience. Gabriela Gutierrez1,2, Dolors Costa1,2,3, Blanca Espinet3, Joan Carles Reverter1,2.1Hospital Clínic, Barcelona, Spain, 2Standardization Committee. Spanish Society of Hematology and Hemotherapy Spain, 3Spanish Cooperative Group for Hematological Cytogenetics Spain Inhibitory Effects of Fish Oil on Platelet-associated Thrombin Generation as Measured by the Calibrated Automated Thrombogram: an in vitro study. Jack Harbert, Kelly Pagano, Denae Heartfield, Michael Ero. Machaon Diagnostics, Oakland, CA, United States Blood is thicker than water. Max Hardeman. Academic Medical Center, Haarlem, Netherlands Primary prevention of Hemoglobinopathies by prenatal diagnosis and selective pregnancy termination in a Muslim country: Oman Suha Hassan1,2, Egbert Bakker1, Cornelis Harteveld1, Piero Giordano1.1Hemoglobinopathies Laboratory, dept. of Clinical Genetics, Leiden University Medical Center, Leiden, Netherlands, 2Molecular Genetic Laboratory, National Genetics Centre, Muscat, Oman An explorative study towards rapid phenotype hemoglobin screening by high resolution mass spectrometry on intact proteins in whole blood. F. Helmich1,2, J.L.J. van Dongen2, H.L. Vader1,2, L. Brunsveld2, P.H.M. Kuijper1.1Máxima Medisch Centrum, Veldhoven, Netherlands, 2Eindhoven University of Technology, Eindhoven, Netherlands Validation of age-related reference ranges for serum thymidine kinase activity in chronic lymphocytic leukemia and its comparison to other prognostic markers Zsuzsanna Hevessy, Eszter Szánthó, Péter AntalSzalmás, Anikó Újfalusi, János Kappelmayer, Harjit Pal Bhattoa. University of Debrecen, Department of Laboratory Medicine, Debrecen, Hungary Thrombophilia Screening as part of Preventive Medicine, a one center experience Etab Higazi1,2, Ophir Avizohar1, Lisa Rubin2, Manfred Green2, Benjamin Brenner1, Galit Sarig1. 1Rambam Health Care Campus, Haifa, Israel, 2School of Public Health, Haifa University, Haifa, Israel The association between HLA-E polymorphisms and NK cell modulate hematopoietic stem cell transplant outcome ehteram hosseini1, Mehran Ghasemzadeh1, Anthony Schwarer2. 1Blood Transfusion Research Center, tehran, Iran, 2Department of Immunology, Monash University, Melbourne, VIC, Australia Australia A Comparison of Two Polymerase Chain ReactionBased Assays of Clonal Immunoglobulin Gene and TCF3-PBX1 Transcripts in the Detection of Minimal Residual Disease in t(1;19) Acute Lymphoblastic Leukemia Ying-Jung Huang1, Lee-Yung Shih1,2, Der-Cherng Liang3, Tang-Her Jaing4, Chih-Cheng Hsiao5, Yu-Hui Huang2, Chang-Liang Lai1, Chia-Hui Chang1. 1Chang Gung Memorial Hospital at Linkou, Taoyuan, Taiwan, 2Chang Gung University, Taoyuan, Taiwan, 3Mackay Memorial Hospital, Taipei, Taiwan, 4Chang Gung Children’s Hospital, Taoyuan, Taiwan, 5Chang Gung Children’s Hospital, Kaohsiung, Taiwan Meningioma 1 (MN1) expression: Refined Risk Stratification in acute myeloid leukemia with normal cytogenetics lamya Ibrahiem1, Salah Aref2, Emad Azmy3. 1Mansoura faculty of medcine, Mansoura, Egypt, 2Mansoura faculty of medicine, mansoura, Egypt, 3Mansoura faculty of medicine, mansoura, Egypt Glucose-6-phosphate dehydrogenase deficiency among children attending the Emergency Paediatric Unit of Usmanu Danfodiyo University Teaching Hospital, Sokoto, Nigeria Isaac Isah1, Abdullahi Mainasara1, Osaro Erhabor1, Samni Omojuyigbe1, Mohammad Dallatu1, Teddy Adias2. 1Usmanu Danfodiyo University, Sokoto, Nigeria, 2Bayelsa State College of Health Technology, Ogbia, Nigeria. , Nigeria Comparison of hemoglobin on RapidPoint 500 blood gas analyser (Siemens) to XE-5000 cell counter (Sysmex). Julie Jacobs, Monique d’Hondt, Anissa Meskal , Sigrid Vermeiren, Steven Weekx, Kristien Ver Elst. GZA hospitals, Antwerp, Belgium The co inheritance of Alpha thalassemia with beta thalassemia and hemoglobinopathies among people referred to Shafa hospital in Ahwaz Mohammad Ali Jalali Far1, Hossein Hosseini2, Najmaldin Saki1, Hamid Galehdari1,3, Mahdieh Nasirshalal2, Amal Saki1. 1Health research institute, Research Center of Thalassemia & Hemoglobinopathy, Ahvaz, Iran, 2Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran, 3Genetic Department of Shahid Chamran University, Ahvaz, Iran Comparison of automated blood cell counts between Advia 120 and Sysmex XT 4000 i in breast cancer patients and healthy controls Faongchat Jarintanan1, Salin Sirudompas2, Kanlaya Sukchaisri2, Putza Chonsawat3, Weerawat Liemmanee4. 1Medical Technology, Rangsit University, Pathum Thani , Thailand, 2Mahavajilalongkorn Thayaburi Hospital, Pathum Thani , Thailand, 3Hospital for Tropical Disease, Bangkok, Thailand, 4Mathmatics Department, Rangsit University, Pathum Thani , Thailand Molecular Basis of Alpha Thalassemia among Qatari children and Adolescents Madeeha Kamal1,2, Shatha Abu-Ssirriya1, Aseel AbuDayya2, Ramin Badii1, Alia Amer1, Marina Kleanthous3. 11 Hamad medical Corporation, Doha, Qatar, 22 WeillCornell-Medical School , Doha, Qatar, 33 Cyprus Institute of Neurology and Genetics, Cyprus, Cyprus What is the difference between APTTand PT test in high hematocrit? - Study using a high hematocrit value modelMasaaki Kanahara1, Tadao Nakano1, Ryouji Katoh1, Hisao Ikeda2. 1Department of Medical Laboratory Science, Faculty of Health Sciences, Junshin Gakuen University, Fukuoka, Japan, 2Department of Cardiology, Kurume University Medical Center, Kurume, Japan Flow cytometric detection of rare events in myelodysplastic syndromes Bettina Kárai1, Judit Bedekovics2, Gábor Méhes2, János Kappelmayer1, Zsuzsanna Hevessy1. 1University of Debrecen, Department of Laboratory Medicine, Debrecen, Hungary, 2University of Debrecen, Department of Pathology, Debrecen, Hungary Diagnostic assistance of complete blood counts and peripheral blood film in diagnosing dengue fever in under resourced laboratories Sehar Khaliq, Lubna Zafar. Fauji foundation hospital, Rawalpindi, Pakistan Hepatosplenic Gamma Delta T- Cell Lymphoma : Clinicopathological features with flow cytometric and cytogenetic evaluation of a series of 23 patients with clinical outcome Afshan Khan1, Pratibha Kadam Amare2, PG Subramanian3, Nikhil Patkar3, Prashant Tembhare3, Shraddha Nikhalge2, Sitaram Goghale3, Ashok Kumar3, Badrinath Y3, Tanuja Shet1, Epari Sridhar1, Shripad Banavali4, Brijesh Aurora4, Hari Menon5, Manju Sengar5, Sumeet Gujral1. 1Department of Pathology, Tata Memorial Hospital, Mumbai, India, 2Department of Cytogenetics, Tata Memorial Hospital, Mumbai, India, 3Haematopatholgy Laboratory, Tata Memorial Hospital, Mumbai, India, 4Department of Paediatric Oncology, Tata Memorial Hospital, Mumbai, India, 5Department of Medical Oncology, Tata Memorial Hospital, Mumbai, India Prenatal diagnosis of Beta thalassemia: A preventive strategy in a developing country saleem khan. armed forces institute of pathology, rawalpindi, Pakistan RDW-CV, IG count and Mo-X obtained from New Automatic Hematology Analyzer Sysmex XN-2000 provide Useful Information for Diagnosis and Prognosis of DIC Bo Hyun Kim, Chan-Jeoung Park, Mi-Jeong Kim, MinYoung Han, Young Jin Kim, Sang Hyuk Park, Young-Uk Cho, Seongsoo Jang, Hyun-Sook Chi Chi. Asan Medical Center, Seoul, South Korea Evaluation of Sysmex XN-2000 and XE-2100 in umbilical cord blood Hanah Kim1, Mina Hur1, Sang-Gyeu Choi1, Hee-Won Moon1, Yeo-Min Yun1, Han Sung Hwang2, Han Sung Kwon2, In Sook Sohn2. 1Departments of Laboratory Medicine, Konkuk University School of Medicine, Seoul, Korea, 2Departments of Obstetrics and Gynecology, Konkuk University School of Medicine, Seoul, Korea The analysis of myeloid-derived suppressor cells in cancer patients with neutropenia to evaluate the effect of granulocyte colony stimulation factor Sun Hyung Kim1, Young Ree Kim1, Sung Ha Kang1, Sanghoon Han2. 1Department of Laboratory Medicine, Jeju National University School of Medicine and Jeju National University Hospital, Jeju, Korea, 2Department of Internal Medicine, Jeju National University School of Medicine and Jeju National University Hospital, Jeju, Korea Therapy-related myeloid neoplasm with a t(5;14)(q33;q32) and PDGFRB rearrangement after treatment for acute promyelocytic leukemia Eunha Koh1, Hungu Kim2. 1Department of Laboratory Medicine, Gyeongsang National University School of Medicine, Jinju, South Korea, 2Department of Internal Medicine Gyeongsang National University School of Medicine, Jinju, South Korea LONGITUDINAL EVALUATION OF HEMATOLOGICAL VALUES OBATAINED WITH REFERENCE AUTOMATED HEMATOLOGY ANALYZERS OF SIX MANUFACTURERES (2013) Hiroshi Kondo1, Yohko Kawai1, Kazuto Tsuruda1, Toshiyuki Ikemoto1, Atsushi Shirakami1, Yutaka Nagai1, Yasunori Kondo1, Mariko Banzai1, Hisataka Ikeda1, Yoko Sugiyama1, Osamu Yonekawa2. 1Blood Cell Count Standardization Subcommittee, Japanese Society for Laboratory Hematology, Tokyo, Japan, 2Japanese Society for Laboratory Hematology, Tokyo, Japan Mean Platelet Volume and Mean Neutrophillic Volume as predictors of the presence and severity of Diabetes Mellitus Praveen Kr, Aashish Gupta, Parul Arora, Subhadra Sharma, Asok Mukhopadhyay. All India Institute Of Medical Sciences, New Delhi, India No need for microscopy or flow cytometry to quantify myelemia in daily haematology laboratory practice. Hazar Lahlou, Ingrid Beukinga, Zaïna Kassengera, Dominique Penninck, Jonathan Brauner, Olivier Pradier. ULB-Hôpital Erasme, Brussels, Belgium Evaluation of a novel Sysmex, cap-piercing, multiwavelength, haemostasis analyser Phil Lane, Andrew Lawrie, Ian Mackie, Sam Machin. Haemostasis Research Unit, University College London, London, United Kingdom Platelet and MegakaryocyteMorphology: A Diagnostic Aid for Inherited Thrombocytopenias: Véronique Latger-Cannard1, Sue Mead2, Odile Fenneteau3, Paquita Nurden4, Thomas Lecompte5. 1Service d’Hématologie Biologique, Chu, Nancy, France, 2Siemens Diagnostics, Reading, United Kingdom, 3Hôpital Robert-Debré, Paris, France, 4Hôpital la Timone, Marseille, France, 5Hematology Division, HUG, Genève, Switzerland New generation ektacytometry for hereditary spherocytosis diagnosis: a six month experience Elena Lazarova1,2, Frédéric Cotton1, Béatrice Gulbis1. 1Erasme Hospital, Department of Clinical Chemistry , Brussels, Belgium, 2Erasme Hospital, Laboratory of Haematology and Transfusion, Brussels, Belgium Overexpressed let-7a-3 is associated with poor outcome in acute myeloid leukemia Jiang Lin, Jun Qian, Yun Li, Jing Yang, Wei Qian, Dongming Yao, Zhao-qun Deng, Qing Liu, Xing-xing Chen, Dong Xie, Cui An, Chun-yan Tang. Affiliated People’s Hospital of Jiangsu , Zhenjiang, China Results From A Standardized Method For Counting Reticulated Platelets In 'Apparently Disease-free' Subjects From 3 International Sites Nigel LLEWELLYN-SMITH1, Benjamin HEDLEY2, Stephen LANG3, Neil MacNAMARA3, David ROSENFELD3, Michael KEENEY2. 1Abbott Diagnostics, Santa Clara, CA, United States, 2London Health Sciences Centre, London, ON, Canada, 3Liverpool Hospital, Liverpool, Australia Inferring APTT results from biphasic coagulation curves in Sysmex coagulation analyzers H.M. Loovers1, K. Bakhtiari2, R. Mulder1, J.H. Nijland1, M.V. Lukens1, A.B. Mulder1, L.J. van Pelt1. 1Dept. of Laboratory Medicine, University Medical Centre, Groningen, Netherlands, 2Dept of experimental vascular medicine, Academic Medical Centre, Amsterdam, Netherlands Bernard-Soulier Syndrome due to a novel homozygous 23-base pair deletion in a native Costa Rican population. Jorge Lopez 1, Walter Cartin1, Marta Navarrete 2, Reinhard Schneppenheim 3.1Specialized Studies and Research Laboratory, National Children´s Hospital, San José , Costa Rica, 2Department of Hematology, National Children´s Hospital, San José, Costa Rica, 3University Medical Center Hamburg-Eppendorf, Hamburg , Germany Enumeration of schistocytes using the DM-96 automated microscope Thomas Maenhout1, Ludo Marcelis1. 1UZ Gent, Gent, Belgium, 2AZ Delta Hospital, Roeselare, Belgium Comparison of immature granulocyte count by the XN series and by microscopic differentiation using DM-96. Thomas Maenhout1, Ludo Marcelis2. 1UZ Gent, Gent, Belgium, 2AZ Delta Hospital, Roeselare, Belgium Why does the Iranian national program of screening newborns for G6PD enzyme deficiency miss a large number of affected infants? Mohammad Reza Mahdavi1, Mehrnoush Kosaryan1, Hossein Jalali1, Payam Roshan2.1Mazandaran University of Medical Science, Sari, Iran, 2Fajr Medical Laboratory, Sari, Iran Iran CONTRIBUTION OF THE PERIPHERAL BLOOD (PB) CELL IMAGES OBTAINED IN THE CELLAVISION DM96 TO THE INFECTIOUS DISEASES DETECTION SERRANDO MAITE1, TEJERINA PATRICIA1, MARULL ANNA1, MERINO ANNA2.1Clinical Laboratory, Hospital Josep Trueta, GIRONA, Spain, 2HaemotherapyHemostasis Service, Hospital Clínic, BARCELONA, Spain Somatic mosaicism causing PK deficiency identified by Next Generation Sequencing: implications for genetic counseling María del Mar Mañú Pereira1, Eva González Roca2, Richard van Wijk3, Hans van Amstel4, Laura Montllor1, Julián Sevilla Navarro5, Juan Lluis Vives Corrons1. 1Red cell pathology Unit Hospital Clínic of Barcelona, Barcelona, Spain, 2Department of Immunology Hospital Clínic of Barcelona, Barcelona, Spain, 3Department of Clinical Chemistry and Haematology, University Medical Center Utrecht, Utrecht, Netherlands, 4Department of Medical Genetics, University Medical Center Utrecht, Utrecht, Netherlands, 5Red cell pathology Unit Hospital Clínic of Barcelona, Barcelona, Spain, 6Hospital Infantil Universitario Niño Jesús, Madrid, Spain, 7Red cell pathology Unit Hospital Clínic of Barcelona, Barcelona, Spain Method Comparison and clinical assessment of biological fluid mode (BF) on the XN-1000 vs XE5000 Sysmex analyzers. Miriam Martínez-Villanueva, Elena Lozoya-Angulo, Ana Martínez-López de Ca, Jose Antonio NogueraVelasco. Clinical Laboratoy Service, Clinical Universitary Hospital Virgen de la Arrixaca, Murcia, Spain, Murcia, Spain Appointing cross-reactivity of both old and new anticoagulation medication on the Sysmex CS2100i Pamela McLaughlin, Marieke Hulsenbos, Jan W Smit, Rob Oude Elferink. Certe locatie Martiniziekhuis, Groningen, Netherlands PERIPHERAL BLOOD PROGENITOR MONONUCLEAR CELL COUNT IN APHERESIS PRODUCT BY TWO METHODS: PRELIMINARY DATA Teresa Molero, Melania Moreno, Angelina Lemes, Carlos Rodriguez, Jezabel López, Luisa Guerra. Hospital Universitario de Gran Canaria Dr Negrín, Las Palmas de Gran Canaria,, Spain HEREDITARY DEFICIENCY OF PYRUVATEKINASE (PKLR) ASSOCIATED WITH CHRONIC HAEMOLYSIS. CONTRIBUTION OF PKLR GENE SEQUENCING TO DIAGNOSIS IN CASES OF HIGH RETICULOCYTOSIS Laura Montllor, María del Mar Mañú Pereira, Pilar Goméz, Jessica Abad, Laura Olaya, Juan LLuis Vives Corrons. Red Cell Pathology Unit. Biomedical Diagnostic Centre-IDIBAPS. University of Barcelona., Barcelona, Spain Assessment of immature platelet fraction and immature reticulocyte fraction as predictors of engraftment of hematopoietic stem cell transplantation Iuri Vicente Morkis, Mariela Farias, Lisandra Rigoni, Luciana Scotti, Lauro Gregianin, Liane Daudt, Lucia Silla, Alessandra Paz. Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil Molecular detection of most internationally reported mutations in Egyptian Fanconi anemia patients Rehab Mosaad1, Maha Eid2, Ghada El-Kamah3. 1NRC, Giza, Egypt, 2NRC, Giza, Egypt, 3NRC, Giza, Egypt D-DIMER REFERENCE VALUES IN MOROCCAN PREGNANT WOMEN MOKAFIH Mounia1, RHOMRI B.1, OUASSISSOU H.2, ENNACHIT M.2, MIKKO F.2, EL KERROUMI M.2, GHAZLI M.2, MATAR N.2, OUKKACH B.1. 1Hematology laboratory Ibn Rochd University Hospital-casablanca, casablanca, Morocco, 2Department of obstetrics and gynecology, IIbn Rochd university hospital , Casablanca, Morocco BCR-ABL fusion transcripts' frequencies in Sudanese CML patients AbdelRahim Muddathir1, Anwar Kordofani2, Imad FadlElmula3. 1Alzaiem Alazhari University, Khartoum, Sudan, 2University of Khartoum, Khartoum, Sudan, 3AL Neelain University Khartoum, Sudan The challenges of haemoglobinopathy screening in a multiethnic population Tracy Murphy, Carrie Van Der Weyden, Rosemary Farmilo, Karen Boudik, Stephen Lang, David Rosenfeld, Michael Harvey, Lye Lin Ho, Penny Motum. Department of Haematology, SSWAPS, Liverpool Hospital, Sydney, Australia Toward a reference method for more accurate reticulocyte counts in blood: Comparison of proposal flow cytometric methods with the reference methods specified to the CLSI H26-A2 standard Yutaka NAGAI1,2, Shigeko Yamamoto2, Yuki Jishage2, Kazunori Chiba2, Etsuko Ogawa3, Hiroshi Kondo1. 1Department of Health Science, Daito Bunka University, Saitama, Japan, 2IVD Technology Center, Nihon Kohden Corporation, Tokyo, Japan, 3BD Bioscience, Tokyo, Japan Performance and Efficiency of CellaVision DM96 for Review of Peripheral Blood Smears Including Red Blood Cell Morphology Megan Nakashima, Terence Doyle, Kathleen PhelanLewin, John Summers, James Bena, Eric Hsi. Cleveland Clinic , Cleveland , OH, United States EXCESS WBC LYSIS IN THE WNR CHANNEL OF XN SERIES (SYSMEX) IS ASSOCIATED WITH METASTATIC ADENOCARCINOMA Phuong NGUYEN, Delphine GOBIN, Rong LI, Brigitte CANTINIEAUX. CHU Saint-Pierre and Institut Jules Bordet, Bruxelles, Belgium Molecular and Serological Assay for RH-DEL Blood Group Variant Detection Pornlada Nuchnoi, Jairak Thongbus, Virapong Prachayasittikul. Mahidol University, Bangkok, Thailand PROTHROMBIN TIME AND ACTIVATED PARTIAL THROMBIN TIME VALUES AMONG GERIATRIC IN NATIONAL HOSPITAL ABUJA NIGERIA Andrew Nuhu1, Amos Dangana2, Ifedayo Ajayi3. 1National Hospital, Abuja, Nigeria, 2University of Abuja Teaching Hospital Gwagwalada, Abuja, Nigeria, 3University of Benin Edo State,Nigeria, Benin, Nigeria An investigation of the protective effect of alpha+thalassaemia against severe Plasmodium falciparum amongst children in Kumasi, Ghana Clement Opoku-Okrah1, Michael Gordge 2, Emmanuel Kweku Nakua2, Tsiri Agbenyega2, Martin Parry2, Claire Robertson2, Caroline Smith2. 1Kwame Nkrumah University of Sci and Tech, Kumasi, Ghana, 2University of Westminster, London, United Kingdom Evaluation of the Mindray BC-6800 and BC-3600 automated hematology analyzers for screening of thalassemia in Southeast Asian populations Kittiphong Paiboonsukwong1, Pornpan Sirankapracha1, Nitsara Wapeesitthiphan1, Pornnapa Khampan1, Wichuda Thamprasert2, Saovaros Svasti1, Pranee Winichagoon1, Suthat Fucharoen1. 1Thalassemia Research Center, Institute of Molecular Biosciences, Mahidol University, Nakornpathom, Thailand, 2Thalassemia Research Center, Institute of Molecular Biosciences, Mahidol University, Nakornpathom, Thailand, 3Thalassemia Research Center, Institute of Molecular Biosciences, Mahidol University, Nakornpathom, Thailand, 4Thalassemia Research Center, Institute of Molecular Biosciences, Mahidol University, Nakornpathom, Thailand, 5Department of Medicine, Nakorn Pathom Hospital, Nakornpathom, Thailand, 6Thalassemia Research Center, Institute of Molecular Biosciences, Mahidol University, Nakornpathom, Thailand, 7Thalassemia Research Center, Institute of Molecular Biosciences, Mahidol University, Nakornpathom, Thailand, 8Thalassemia Research Center, Institute of Molecular Biosciences, Mahidol University, Nakornpathom, Thailand Platelet indices, a useful tool in the differential diagnosis of low platelet counts. Katerina Palla, Nektarios Proukakis, Katerina Zouridi, Kasiani Tzagaraki. general hospital of chania, chania, Greece Sepsis affects most routine and cell population data (CPD) obtained using the Sysmex XN-2000 blood cell analyzer: Neutrophil-related CPD NE-SFL and NEWYprovide useful information for detecting sepsis Sang Hyuk Park, Chan-Jeoung Park, Bo-Ra Lee, Ki-Sun Nam, Mi-Jeong Kim, Min-Young Han, Young Jin Kim, Young-Uk Cho, Seongsoo Jang. Department of Laboratory Medicine, University of Ulsan, College of Medicine and Asan Medical Center, Seoul, South Korea Mathematical Model-based Estimation of Red Blood Cell Clearance Predicts Future Anemia in Patients with Normal Complete Blood Counts Harsh Patel1, John M. Higgins1,2. 1Center for Systems Biology: Massachusetts General Hospital, Boston, MA, United States, 2Department of Systems Biology: Harvard Medical School, Boston, MA, United States Eosinophilia in a Veterans Affairs Medical Center (VAMC). Eugene S. Pearlman1, Ashley Porter2, Megan Kempe2, Judy Layden1. 1VAMC, Memphis, TN, United States, 2University of Tennessee Health Sciences Center, Memphis, TN, United States Linking Testing-to-Clinical Goals – A Risk Stratified Process Redesign Scheme Towards Improving Manual Blood Film Review (MBFR) Services in a Large Tertiary Hospital Laboratory DAVID PI, MONIKA HUDOBA, JAMES YAKIMEC, TODD MARKIN, HABIB RAZAVI, ALYM ABDULLA, JINGJIAO HE. Vancouver General Hospital, Vancouver, BC, Canada An innovative approach for detecting red blood cell agglutinations with BC-6800 hematology analyzers Huan Qi, Zhaoyang Li, Bo Ye, Guanzhen Wang, Jin Li, Cheng Qian. Mindray Medical International , Shenzhen, Guangdong, China Hypomethylation of DDX43promoter predicts a favorable outcomein acute myeloid leukemia Jun Qian, Jiang Lin, Qin Chen, Zhao-qun Deng, Wei Qian, Dong-ming Yao, Ji-chun Ma, Jing Yang, Hai-yan Chai, Yun Li, Qing Liu, Yu-juan Ma. Affiliated People’s Hospital of Jiangsu , Zhenjiang, China The role of immunophenotype and cytogenetics on survival of primary multiple myeloma cells treated by arsenic trioxide Katarina Reberšek, Peter Černelč, Helena Podgornik. University medical Centre, Ljubljana Slovenia Inter-laboratory reproducibility of digital blood morphology Jurgen Riedl. Albert Schweitzer Hospital, Dordrecht, Netherlands Digital morphology: a proven concept Jurgen Riedl. Albert Schweitzer Hospital, Dordrecht, Netherlands ALTERATIONS IN HEREDITARY SPHEROCYTOSIS AND ELLIPTOCYTOSIS ANALYZED BY SCANNING ELECTRON MICROSCOPY AND RHEOLOGICAL TECHNIQUES Bibiana Riquelme1,2, Irma L Acosta2, Susana Perez2, Cintia Elias2, Martina Avalos1, Angela Milani2. 1Instituto de Física Rosario, Rosario, Argentina, 2Facultad de Cs. Bioquímicas y Farmacéuticas, (UNR), Rosario, Argentina A simple functional assay for Heparin-Induced Thrombocytopenia testing Niels Riteco1, Sophie Rochat1, Sophie Lee2, Simon Jones2, Lorenzo Alberio1, George Chan2,3. 1Department of Hematology and Central Hematology Laboratory, Inselspital, Bern University Hospital and University of Bern, Bern, Switzerland, 2Department of Haematology, Auckland City Hospital, Auckland, New Zealand, 3Department of Molecular Medicine and Pathology, University of Auckland, Auckland New Zealand ECCIC STUDY: STUDY OF UNEXPLAINED CYTOPENIAS BY CYTOMORPHOLOGY, CYTOGENETICS AND FLOW CYTOMETRY Ines Rodríguez-Hernández, Alicia Castillo, José-Tomás Navarro, Jordi Juncà, Isabel Granada, Sara Vergara, Minerva Raya, Ester Viñets, Evarist Feliu, Fuensanta Millá.Hematology Department, Institut Català d’Oncologia, Hospital Germans Trias i Pujol, Institut de Recerca contra la Leucèmia Josep Carreras, Universitat Autònoma de Barcelona. , Badalona, Spain Expression and sub-cellular localisation of NFkappaB in lymphoproliferative diseases: optimisation of an antibody panel for imaging flow cytometry. Kimberley J Roehrig1, Lizz F Grimwade2, Kathy Heel1, Dian A Teguh1, Mike A Scott2, Mars B van 't Veer2, Jill Finlayson3, Wendy N Erber1. 1School of Pathology and Laboratory Medicine, The University of Western Australia, Crawley, Australia, 2Haemato-Oncology Diagnostic Service, Haematology Department, Cambridge University Hospitals NHS Foundation Trust, Cambridge, United Kingdom, 3Department of Haematology, PathWest Laboratory Medicine, Perth, Australia Electropherogram patterns of Hb Constant Spring and Hb Pakse on capillary zone electrophoresis Nattaya Sae-ung, Sivanon Thermsook , Sukhum Srijumnong, Goonapa Fucharoen, Supan Fucharoen. Centre of Research and Development of Medical Diagnostic Laboratories, Khon Kaen University, Khon Kaen, Thailand The association of FXIII val34leu polymorphism with cerebrovascular thrombosis in Iranian patients Seyed Mehdi Sajadi1, Hamzeh Rahimi2, shahram samiei3, maryam kheirandish4, Zahra Ataiei5, Mahnaz Kavari6, Maryam Abdollahi7. 1Birjand university of medical sciences, Birjand, Iran, 2molecular medicine, Pasteur institute of Iran, Tehran, Iran, 3Iranian blood transfusion research center, Tehran, Iran, 4Iranian blood transfusion research center, Tabriz, Iran, 5Iranian blood transfusion research center, Tehran, Iran, 6Iranian blood transfusion research center, Tehran, Iran, 7Iranian blood transfusion research center, Tehran, Iran Prevalence and prognostic value of IDH1 R132 mutation in newly diagnosed AML Egyptian patients with normal karyotype Dalia Salem1, Sherine Abdel Aziz1, Nadia Elmenshawy1, Tarek Abouzid2, Mohamed Ebrahim3. 1Clinical Pathology Department, Mansoura Faculty of Medicine, Mansoura, Egypt, 2Clinical Hematology Unit, Oncology Center, Mansoura Faculty of Medicine, Mansoura, Egypt, 3Medical Oncology Unit, Oncology Center, Mansoura Faculty of Medicine, Mansoura, Egypt Flow Cytometry of Bone Marrow Aspirates in the Diagnosis of Myelodysplastic Syndrome Roger Schindhelm, Mark De Jong, Ellen Kramer. Isala klinieken, Zwolle, Netherlands The molecular-genetics mechanisms of platelets activation in patients with cerebrovascular disease Olga Sirotkina1,2,3, Anastasiya Laskovets3, Vitaliy Goldobin3, Tatiana Vavilova2,3.1B.P. Konstantinov Petersburg Nuclear Physics Institute, Saint-Petersburg, Russia, 2Federal Almazov Medical Research Centre, Saint-Petersburg, Russia, 3North-Western State Medical University named after I.I. Mechnikov, Saint-Petersburg, Russia A Comparison of High Resolution Melting, Allelespecific Priming and Sanger Sequencing for the Detection of BRAFV600E Mutation in Hairy Cell LeukaemiafromDifferent Haematological Specimens Chi-Chiu So1, Amy Chan2, Lap-Ping Chung2. 1Department of Pathology, University of Hong Kong, Hong Kong, China, 2Department of Pathology and Clinical Biochemistry, Hong Kong, China STANDARDIzing PRE-ANALYTICAL STAGE OF BLOOD SMEAR PREPARATION. IMPORTANCE FOR AUTOMATIC ANALYSIS SYSTEMS Dmitry Sosnin, Olga Nenasheva, Oleg Kubarev. Perm State Medical Academy , Пермь, Russia Six-day stability of reticulocyte and erythrocyte parameters in-vitro: a comparison of blood samples from healthy, iron deficient and thalassemic individuals Åshild A. Sudmann-Day1, Armin Piehler2, Olav Klingenberg1, Petter Urdal1.1Department of Medical Biochemistry, Oslo University hospital, Oslo, Norway, 2Fürst Medical Laboratory, Oslo, Norway Performance Evaluation of the Integrated Slide Processing System Sysmex DI-60 Yamamoto Takamasa1, Yoko Tabe2, Imiko Maenou1, Rie Nakai3, Mayumi Idei2, Takashi Horii1, Takashi Miida2, Akimichi Ohsaka1. 1Juntendo University Hospital, Tokyo, Japan, 2Juntendo University School of Medicine, Tokyo, Japan, 3Sysmex Corporation, Hyogo, Japan Age-Based Difference in Heparin Monitoring in Pediatric ECMO Jun Teruya, Shilpa Hundalani, Vadim Kostousov. Texas Children's Hospital and Baylor College of Medicine, Houston, TX, United States The First Step to Establish External Quality Assessment Scheme for Point-of-Care INR in Thailand Panutsaya Tientadakul, Achara Chuntarut. Faculty of Medicine Siriraj Hospital, Mahidol University , Bangkok, Thailand Impact of the proton pump inhibitors on platelet response to clopidogrel A. Anil Timur1, Gurunathan Murugesan1, John Barnard2, Sanjay Gandhi3, Deepak L. Bhatt4, Kandice KottkeMarchant1. 1Cleveland Clinic, Robert J. Tomsich Pathology and Laboratory Medicine Institute, Cleveland, OH, United States, 2Cleveland Clinic, Quantitative Health Sciences, Cleveland, OH, United States, 3MetroHealth Campus-Case Western Reserve University, Heart and Vascular Center, Cleveland, OH, United States, 4Brigham and Women's Hospital, and Harvard Medical School, Boston, MA, United States Erythrocyte subsets in the differential diagnosis of microcytic anemia ELOISA URRECHAGA1, Johannes JML Hoffmann2, Luis Borque3, Jesus F Escanero3.1HOSPITAL GALDAKAO USANSOLO, GALDAKAO VIZCAYA, Spain, 2Medical & Scientific Affairs, Abbott Diagnostics, Abbott GmbH & Co, Wiesbaden-Delkenheim, Germany, 3Department of Pharmacology and Physiology, Faculty of Medicine, University of Zaragoza, Zaragoza, Spain Erythrocyte and reticulocyte indices by CELL-DYN Sapphire in Hemodialysis patients ELOISA URRECHAGA1, Johannes Hoffmann2, Luis Borque3, Jesus Escanero3.1HOSPITAL GALDAKAO USANSOLO, GALDAKAO VIZCAYA, Spain, 2Medical & Scientific Affairs, Abbott Diagnostics, WiesbadenDelkenheim, Germany, 3Department of Pharmacology and Physiology, Faculty of Medicine, University of Zaragoza, Zaragoza, Spain Reticulocyte Hb equivalent and hypochromic red cells in the detection of latent iron deficiency in non-anemic premenopausal women ELOISA URRECHAGA1, Elia Crespo1, Luis Borque2, Jesus Escanero2. 1HOSPITAL GALDAKAO USANSOLO, GALDAKAO VIZCAYA, Spain, 2Department of Pharmacology and Physiology Faculty of Medicine. University of Zaragoza., Zaragoza, Spain The SKML hemocytometry external quality control (EQC) as an external quality control for flow cytometric leukocyte differentiation by Cytodiff tm. Gert-Jan van de Geijn1, Andre van Rossum 2, Guido Lansbergen3, 4, Jeffrey Keuren3,4, Karin Mohrmann5, Feitse Jan Duisterwinkel6, Adrian Kruit6, Nannette Brouwer7, Gerard Beukeveld8, Sjef van de Leur9, Geert Weijers10, Tjin Njo1. 1Sint Franciscus Gasthuis, Rotterdam, Netherlands, 2Bronovo Hospital, Den Haag, Netherlands, 3Zuwe Hofpoort Hospital, Woerden, Netherlands, 4Groene Hart Hospital, Gouda, Netherlands, 5SHL-group, Etten-Leur, Netherlands, 6Nij Smellinghe Hospital, Drachten, Netherlands, 7't Lange Land Hospital, Zoetermeer, Netherlands, 8Westfries Gasthuis Hospital, Hoorn, Netherlands, 9Isala Hospital, Zwolle, Netherlands, 10Beckman Coulter, Woerden, Netherlands Quantification of BCR-ABL1 transcripts on bone marrow samples using the GeneXpert based V1 cartridge system Christine Van Laer, Jan De Roover, Bieke Wilmsen, Katrien Bruyninckx, Nancy Boeckx. UZ Leuven, Leuven, Belgium Expedience of the Green and King rule implemented in the hematology-analyser software for screening for Hb-pathies Edwin van Mire. MDC-Amstelland, Amstelveen, Netherlands Hemostatic function in thyroid disorders Tatiana Vavilova1, Elizaveta Egorova2, Andrey Ivanov2. 1Federal Almazov Medical Research Center, Saint-Petersburg, Russia, 2S.M.Kirov Military Medical Academy, Saint-Petersburg, Russia FETAL HEMOGLOBIN DETERMINES THE DEGREE OF ANISOCYTOSIS IN BETA AND DELTA BETA THALASSAEMIA TRAIT Diego Velasco-Rodríguez1, Juan Manuel AlonsoDomínguez1, Fernando Ataúlfo González-Fernández1,2, Raquel Guillén1, Paloma Ropero2, Natalia Acedo1, Cristina Serí1, Beatriz Álvarez1, Jesús Villarrubia1, Fernando Cava1. 1Laboratorio Central de la Comunidad de Madrid (Unilabs, BR Salud), Madrid, Spain, 2Hospital Clínico San Carlos, Madrid, Spain ANALYSIS OF RETICULOCYTE PARAMETERS IN HEMOGLOBIN S TRAIT AND HEMOGLOBIN C TRAIT Diego Velasco-Rodríguez1, Juan Manuel AlonsoDomínguez1, Fernando Ataúlfo González-Fernández1,2, Paloma Ropero2, Raquel Guillén1, Cristina Serí1, Natalia Acedo1, Beatriz Álvarez1, Jesús Villarrubia1, Fernando Cava1. 1Laboratorio Central de la Comunidad de Madrid (Unilabs, BR Salud), Madrid, Spain, 2Hospital Clínico San Carlos, Madrid, Spain FACTORS THAT INFLUENCE THE RATE OF MANUAL VALIDATION IN THE LABORATORY OF HEMATOLOGY Diego Velasco-Rodríguez, Marta Jiménez-Rolando, Juan Manuel Alonso-Domínguez, Raquel Guillén, Jesús Villarrubia, Fernando Ataúlfo González-Fernández, Fernando Cava. Laboratorio Central de la Comunidad de Madrid (Unilabs, BR Salud), Madrid, Spain CONGENITAL HEXOKINASE (HK1) DEFICIENCY ASSOCIATED WITH SEVERE NEONATAL HAEMOLYTIC ANAEMIA DUE TO A NOVEL HK1 GENE MUTATION. USEFULNESS OF HK/PK RATIO FOR THE DIAGNOSIS Juan LLuis Vives Corrons1, María del Mar Mañú Pereira1, Pilar Goméz1, Laura Olaya1, Richard Van Wijk2. 1Red Cell Pathology Unit. Hospital Clinic. University of Barcelona., Barcelona, Spain, 2Department of Clinical Chemistry and Haematology. University Medical Center of Utrecht, Utrecht, Netherlands Improvement of the leukocyte differential performed by flow cytometry using the advanced 2.0 version of the CytoDiff CXP software Orianne Wagner-Ballon1,2, Antoine Cottard3, Jérome Le Priol3, Beatrice Ly-Sunarram3, Thierry Fest3,4, Mikael Roussel3,4. 1Département d'Hématologie et d'Immunologie biologiques, Hôpitaux Universitaires Henri Mondor, APHP, Créteil, France, 2UPEC, Créteil, France, 3Laboratoire d'Hématologie, CHU Rennes, Rennes, France, 4INSERM U917, Rennes, France SF3B1 mutation is a rare event in Chinese patients with acute and chronic myeloid leukemia Jing Yang1, Jun Qian1, Dong-ming Yao1, Si-xuan Qian2, Wei Qian1, Jiang Lin1, Gao-fei Xiao1, Cui-zhu Wang1, Zhao-qun Deng1, Ji-chun Ma1, Xing-xing Chen1. 1Affiliated People’s Hospital of Jiangsu , Zhenjiang, China, 2The First Affiliated Hospital of Nanjing Medical Hospital, Nanjing, China Haemostatic abnormality associated with deep vein thrombosis in Sudanese patients. alfatih aboalbasher yousif,MSc,MT(ASCPi)1, Elwaleed Elamin,PhD2, Abdelrahim Muddathir,PhD3. 1Al Zaiem Al Azhari University Department of hematology and blood transfusion , khartoum Sudan, 2Al Zaiem Al Azhari University faculty of medical laboratory sciences , khartoum Sudan, 3Al Zaiem Al Azhari University Department of hematology and blood transfusion , khartoum Sudan Better detection of Ig and T-cell receptor gene rearrangement in cell-free plasma DNA from patients with acute myeloid leukemia and its implications for minimal residual disease ling zhong, Yanxing LI, Wenfang HUANG. Department of Clinical Laboratory, Sichuan Academy of Medical Science﹠Sichuan Provincial Peoples’ Hospital, chengdu , China Sticky platelet syndrome and platelet expression of CD-62P Ingrid Škorňová1, Jana Fedorová2, Daniela Chudá3, Ján Staško1, Pavol Hollý1, Peter Kubisz1. 1Comenius University, JLF Martin, Martin, Slovakia, 2Hemo-Medika, s.r.o., Martin, Slovakia, 3National Transfusion Service SR s.r.o., Martin, Slovakia International Society of Laboratory Hematology 2111 Chestnut Ave. Suite 145 Glenview, IL 60025 USA