CURRICULUM VITAE
Madhuree Kumari, PhD
Division of Plant Microbes Interaction,
CSIR-National Botanical Research Institute, Rana Pratap Marg,
Lucknow-226 001 (U.P.), India
Mobile: +91-7376065968, +91-9535241073
E-mail: madhuree88@gmail.com
Career objective
To solve exciting problems and pursue cutting edge research in nanomaterials for
development of efficient and affordable technologies.
Area of specialization: Nanomaterials, Microbiology, Proteomics,
Molecular Biology, Plant Pathology, Soil Biology and Biotechnology
Metabolomics,
Educational qualifications:
1. Doctor of Philosophy (Ph. D.) in Life Sciences (awarded on 16-09-2015 in Academy of
Scientific & Innovative Research, CSIR-NBRI)
Thesis title: “Characterization of nanoparticles biosynthesized by Trichoderma
spp. and their utility in agriculture.”
Supervisor: Dr. C. S. Nautiyal, Director, CSIR- National Botanical Research Institute, India
2. Master of Science (M. Sc.) in Biotechnology (2010). Banasthali University,
Banasthali, Rajasthan. 79%.
3. Bachelor of Science (B. Sc.) in Biotechnology (2008). Ranchi University, Ranchi
(Jharkhand) 77.9%.
Experience: 4 years 8 months research experience in nanoparticles biosynthesis,
manipulation of morphologies by plant growth promoting microbes and plant- pathogennanoparticle interactions at molecular level.
I had joined CSIR-NBRI under supervision of Dr C. S. Nautiyal, Director as CSIR-Junior
Research Fellow on 28th Sept, 2010 at Division of Plant Microbes Interaction, CSIR-National
Botanical Research Institute (NBRI), Lucknow, (U.P.), India. I have worked on extracellular
biosynthesis of nanoparticles, their characterization and their utility as antimicrobial agent,
and a superior catalyst. My work focussed on modulation of shapes and sizes of nanoparticles
by providing them unique combination of growth parameters (concentration of cell free
extract and salt, pH, temperature and reaction time). Further a mechanism was also proposed
to elucidate the interdependent role of different biological and physical parameter to generate
different shapes and sizes. My work also includes utilization of biosynthesized nanoparticles
as superior pesticide as compared to their chemical counterpart in model plant system
Arabidopsis thaliana and model mice system. I have expertise in proteomics and
metabolomics to decipher the plants responses during tripartite interaction of plant-pathogennanoparticles. I have expertise in conducting field trials with host plant rice, maize, tomato,
chickpea, wheat and A. thaliana against a range of plant pathogens including Pseudomonas
syringae, Alternaria solani, Alternaria brassicicola, Fusarium oxysporum, Rhizoctonia
solani and Sclerotium rolfsii.
Scientific Skills:
I have expertise in following microbiology and biotechnological techniques:
Nanoparticle Biosynthesis, morphology manipulation, Characterization: Biosynthesis of
metal nanoparticles from different bacteria and Trichoderma spp. Characterization of
particles by UV-vis spectroscopy, transverse electron microscope (TEM) and scanning
electron microscope (SEM), Energy dispersive X ray analysis (EDAX), X ray diffraction
(XRD), and Fourier transform infrared spectroscopy (FT-IR). Manipulation of shapes and
sizes of nanoparticles by varying biological and physical parameters. Biosynthesis of
nanoparticles of different shapes (spheres, pseudospheres, penta and hexagons, triangles and
prism, rectangles and sheets). Effect of different morphologies upon catalytic and
antimicrobial properties of nanoparticles
Microbiology: Screening and isolation of microbes (bacteria, fungi, algae) from different
ecological sites, maintenance and preservation of microbial cultures, scale up of microbial
cultures qualitative and quantitative estimation of plant growth promoting attributes,
preparation of biofertrilizer and biopesticides, nanobiopesticides, BIOLOG analysis,
microbial biofilm formation, field trials with pathogens, disease diagnosis, disease index
calculation. Use of nanoparticles against MDR bacteria, effect of different capping agents on
nanoparticles in imparting antimicrobial activity.
Proteomics: Two dimensional gel electrophoresis, data analysis, sample preparation for
MALDI-TOF-TOF, data analysis and interpretation of MALDI-TOF-TOF.
Metabolomics: HPLC, and Data analysis of GC-MS and HPLC.
Molecular Biology: Total genomic DNA/RNA isolation of plant, microbes, and plasmids
isolation, Polymerase Chain Reaction (PCR), Nested-PCR, RT-PCR, Gel electrophoresis,
Restriction Digestion, Molecular cloning in cloning and expression vectors, RAPD, RFLP,
Sequencing, Denaturating gradient gel electrophoresis (DGGE)
Histopathological Analysis: Handling of light microscope, staining, analysis and data
interpretation of the images of transverse electron microscope (TEM) and scanning electron
microscope (SEM).
Soil Biology: Soil enzyme analysis, total soil organic carbon estimation, BIOLOG, DGGE,
metagenomics DNA/RNA isolation.
Staistical softwares: Microsoft excel, Prism, SPSS, Statistica, Multi Experiment Viewer
(MEV)
Other: Good knowledge in nucleotide, amino acid sequence analysis using bioinformatics
tools likes NCBI Data Base, EMBL Data Base, Primer designing, Sequence analysis, ,
BLAST, multiple sequence alignment, and various other tools for genome analysis.
Formulation of creams for topical application on skin.
Professional achievements:
 Characteization of nanoparticles synthesized biologically and chemically.
 Developed protocols for biosynthesis of gold and silver nanoparticles of various shapes
and sizes which demonstrated shape and size dependent antimicrobial and catalytic
activities. Explored morphology dependent synergistic effects of nanoparticles with
antibiotics against multidrug resistant animal and plant pathogens.
 Study of proteomic and metabolomic alterations occurred in model plant A. thaliana
during tripartite interaction of plant-pathogen and nanoparticles.
 Expertise in screening, isolation and identification of microbes to be used as biofertilizers,
biopesticides and biofactory for nanoparticles synthesis.
 Histopathological analysis by Electron microscope and Light microscope.
Project/ Dissertation completed:
1. Five months dissertation on “Biochemical biomarkers of oxidative stress in Phormidium
tenue (C.Agardh) exposed to Mercury" from National Institute of Oceanography (NIO), Goa
from Jan- May 2010.
Academic Achievments:
1. Qualified NET JRF- December-2009 (Rank 0159) conducted by CSIR-UGC against Roll
No. 319058.
2. Qualified GATE 2010 (Life Sciences) examination with 94.2 percentile
3. Got best publication award for year 2014-2015 in CSIR-NBRI for the paper published in
Bioresource technology.
Personal information
Husband’s name
:
Father’s Name
Mother’s Name
Date of Birth
Gender
Nationality
Languages known
Address for correspondence
:
:
:
:
:
:
:
Hobbies
Mobile
Email ID
:
:
:
Mr. Anup Prasad,
Scientist-C, CEMILAC, DRDO,
Bangalore, India
Mr. Upendra Prasad
Mrs. Neelam Prasad
17-June-1988
Female
Indian
English, Hindi
SH 5/93, DRDO Township phase II,
Kaggadaspura, C V Raman Nagar, Bangalore,
560093.
Writing poem, Listening to music and Reading
+91-9535241073
madhuree88@gmail.com,
madhureenbri@rediffmail.com
Referees:
Dr. C. S. Nautiyal,
FNA, FNASc, FNAAS, J C Bose National Fellow,
Director,
CSIR-National Botanical Research Institute Lucknow-226 001, (U.P.), India
E-mail: csnnbri@yahoo.com
Dr. Aradhana Mishra
Senior Scientist
Division of Plant Microbes Interaction
CSIR-National Botanical Research Institute Lucknow-226 001, (U.P.), India
E-mail: mishramyco@yahoo.com
Dr. Bhumi Nath Tripathi
Associate Professor, Department of Botany
Guru Ghasidas University
Bilaspur, Chhatisgarh, India
E mail: bhuminathtripathi@hotmail.com
DECLARATION
I hereby declare that the information furnished above is true to the best of my knowledge and
I am solely responsible for its accuracy.
Madhuree Kumari
Place: Bangalore
LIST OF PUBLICATIONS
Research publication in International Journals:
1. Mishra, A., Kumari, M., Pandey, S., Chaudhary, V., Gupta, K. C., Nautiyal, C. S.,
Biocatalytic and antimicrobial activities of gold nanoparticles synthesized by
Trichoderma sp. Bioresource Technology, 2014,166, 235-242.
IF: 5.03
2. Mishra, S., Mishra, A., Chauhan P. S., Mishra S. K., Kumari, M., Niranjan, A., Nautiyal,
C. S., Pseudomonas putida NBRIC19 dihydrolipoamidesuccinyltransferase (Suc B) gene
controls degradation of toxic allelochemicals produced by Parthenium
hysterophorus.Journal of Applied Microbiology. 2012, 112, 793-808.
IF: 2.4
Communicated manuscripts:
1. Kumari, M., Pandey, S., Bhattacharya, A., Shukla, R., Mishra, A., Nautiyal, C. S., 2015.
Enhancement in potential of antibiotics against MDR bacteria and antimicrobial efficacy
by tailoring shapes and sizes of biogenic silver nanoparticles. Colloids and Surfaces B:
Biointerfaces.
2. Kumari, M., Pandey, S., Singh, S. P., Mishra, A., Nautiyal, C. S., 2015.Unique balance
of interactions of physical parameters in regulating morphologies of biogenic gold
nanoparticles (GNP) for enhancing catalytic activity. ACS Appl Mat Interface.
Conferences / workshops attended:
1. Attended workshop on Feb 8-9, 2013 on ‘Bioinformatical approaches for protein
sequence analyses and structure prediction’ at department of Biochemistry, University of
Lucknow, Lucknow.
2. Attended national seminar on Nov 29-30, 2013 on ‘emerging trends in
Nanobiotechnology, Jaipur National University, Jaipur, Rajasthan.
3. Presented poster entitled ‘Reduced cell wall degradation plays a role in cow dungmediated management of wilt complex disease of chickpea’ in national seminar
organised by CSIR-CIMAP and CSIR-NBRI in 2014 at CIMAP, Luckmow.
4. Presented poster entitled ‘ Green and instant synthesis of gold nanoparticles by
Trichoderma sp. and its heterogeneous catalysis in degradadtion of 4- nitrophenol and
got second prize in fifth international conference on plants and environmental pollution
organised by ISEB and CSIR-NBRI, Lucknow on Feb 24-27, 2015.