Supporting information: Figure S1: a. Distribution of 1Cx-values found across Melanthiaceae, showing admixture populations skewed towards both ends of the range. b. Representative fluorescence histogram of the genome size assessment for Veratrum fimbriatum using Pisum sativum ‘Ctirad’ [1: mean G1 (2C) peak of the target sample, 2 and 3: mean G1 (2C) and G2 (4C) peaks of the internal standard]. Figure S2: Fifty percent majority-rule consensus tree from Bayesian inference based on the trnT-trnF dataset. Supported branches (PP ≥ 0.95) are indicated in bold. a. Ancestral state reconstruction conducted under ML using categorized 1Cx-values. b. Ancestral state reconstruction conducted under MP using categorized 1Cx-values. Pie charts indicate the relative likelihood of the reconstructed character states. Figure S3: Ancestral chromosome numbers and evolution in Melanthiaceae inferred under ML optimization. Figure S4: Karyotypes and idiograms of Pseudotrillium rivale (a), Paris thibetica (b) and Trillium ovatum (c). Scale bars = 10 μm. Supporting Information Table S1: List of species used in the present study, chromosome numbers with previous reports, voucher information and Genbank/EMBL accession numbers. Standard herbarium acronyms from Index Herbariorum. AJB = Atlanta Botanical Garden, BCN = Botanical Institute of Barcelona, K= Royal Botanic Gardens, Kew, UCBG = University of California Botanical Garden. Note that underlined species were not included in the phylogenetic and statistical analyses. Taxa (2n)a Previous chromosome counts. Reference Voucher- Kew Liv. Coll. trnL-trnF matK BCN201156 HG475351 AB040196 K20031588 HG475352 AB040198 K38704/ K19962547 HG475361 AB017375 K201143 HG475355 AB040186 K20102438/K201141 HG475359 AB109327 K20102440/K2010729 HG475354 AB040187 K38706/K19794837 HG47535 AB040188 K19861707/K20102436 HG475356 n/a Tribe Chionographideae Chamaelirium luteum 24 Chionographis japonica 24* -Silliman, F. E. 1957. Chamaelirium luteum (L.) Gray: A Biological Study. Ph.D. dissertation. University of North Carolina. -Tanaka, N. Y. & N. Tanaka. 1980. Chromosome studies in Chionographis (Liliaceae). III. The mode of meiosis. Cytologia 45: 809-817. Tribe Heloniadeae Helonias bullata 34* Heloniopsis kawanoi 34* Heloniopsis koreana 34* Heloniopsis leucantha 34* Heloniopsis orientalis 34* Heloniopsis orientalis var. breviscapa 34* -Kokubugata, G., C.-I. Peng & M. Yokota. 2004. Comparison of karyotypes among three Heloniopsis species (Liliaceae) from Ryukyu Archipelago and Taiwan. Ann. Tsukuba Bot. Gard. 23: 13–16. -Utech, F.H. 1980. Somatic karyotype analysis of Helonias bullata L. (Liliaceae), with a comparison to the asian Heloniopsis orientalis (Thunb.) C. Tanaka. Annals of Carnegie Museum. 49: 153-160. -Kokubugata, G., C.-I. Peng & M. Yokota. 2004. Comparison of karyotypes among three Heloniopsis species (Liliaceae) from Ryukyu Archipelago and Taiwan. Ann. Tsukuba Bot. Gard. 23: 13–16. -Kokubugata, G., C.-I. Peng & M. Yokota. 2004. Comparison of karyotypes among three Heloniopsis species (Liliaceae) from Ryukyu Archipelago and Taiwan. Ann. Tsukuba Bot. Gard. 23: 13–16. -Kurosawa, S. 1982. Cytotaxonomical studies on the flowering plants of the Oze district. Pp. 135–139. In Hara, H. (editor), Ozegahara[:] Scientific Researches on the Highmoor in Central Japan. Japan Society for the Promotion of Science, Tokyo. -Nishikawa, T. 1989. Chromosome counts of flowering plants of Hokkaido (12). J. Hokkaido Univ. Educ., Sect. 2B 40: 37–48. -Kurosawa, S. 1982. Cytotaxonomical studies on the flowering plants of the Oze district. Pp. 135–139. In Hara, H. (editor), Ozegahara[:] Scientific Researches on the Highmoor in Central Japan. Japan Society for the Promotion of Science, Tokyo. -Nishikawa, T. 1989. Chromosome counts of flowering plants of Hokkaido (12). J. Hokkaido Univ. Educ., Sect. 2B 40: 37–48. Heloniopsis orientalis var. flavida 34* Heloniopsis tubiflora 34* Heloniopsis umbellata 34* Ypsilandra cavaleriei 34* -Kurosawa, S. 1982. Cytotaxonomical studies on the flowering plants of the Oze district. Pp. 135–139. In Hara, H. (editor), Ozegahara[:] Scientific Researches on the Highmoor in Central Japan. Japan Society for the Promotion of Science, Tokyo. -Nishikawa, T. 1989. Chromosome counts of flowering plants of Hokkaido (12). J. Hokkaido Univ. Educ., Sect. 2B 40: 37–48. -Kokubugata, G., C.-I. Peng & M. Yokota. 2004. Comparison of karyotypes among three Heloniopsis species (Liliaceae) from Ryukyu Archipelago and Taiwan. Ann. Tsukuba Bot. Gard. 23: 13–16. - Ypsilandra thibetica 34* -Shu, T. R. 2000. Ypsilandra. In Flora of China 24:86-87 K20102439 HG475357 n/a K20102442 HG475360 AB109323 K20102441/K20102437 HG475353 AB040195 K2010728 HG475362 HG475396 K2010727 HG475363 AB040195 BCN201157 HG475347 n/a Wiebolt s.n. (GA) AF303694 n/a K1980159 AF303697 n/a K20102427 HG475348 n/a Forbes s.n. (UCBG98.0269) (GA) HG475350 n/a Wendt 7016 (TEX) AF303672 n/a Carter20234 (GA) (VSC) AF303690 n/a Zomlefer2429 (GA) AF303692 n/a Zomlefer2421 (GA) AF303691 n/a Tribe Melanthieae Amianthium muscitoxicum 32 Anticlea elegans var. glaucus 32 Anticlea occidentalis 16* Anticlea volcanica 32* Schoenocaulon macrocarpum 16 Schoenocaulon texanum 16 Stenanthium densum 20 Stenanthium gramineum 20* Stenanthium leimanthoides 20 -Zomlefer, W.B. & Smith, G.L. 2002. Documented chromosome numbers 2002: 1. Chromosome number of Stenanthium (Liliales: Melanthiaceae) and its significance in the taxonomy of tribe Melanthieae. Sida 20(1): 221-226. -Love, A. & D. Love. 1981c. In Chromosome number reports LXXIII. Taxon 30: 845–851. -Chinnappa, C. C. & J. G. Chmielewski. 1987. Documented plant chromosome numbers 1987: 1. Miscellaneous counts from western North America. Sida 12: 409–417. -Niehans, F., Wong, L. 1971. In IOPB chromosome reports XXXII. Taxon 20: 349-356. - Powell, A. M. & B. L. Turner. 2005. Documented chromosome numbers 2005: 1. Miscellaneous counts from western Texas, mostly trans-Pecos. Sida 21(3): 1663–1664. -Powell, A. M. & B. L. Turner. 2005. Documented chromosome numbers 2005: 1. Miscellaneous counts from western Texas, mostly trans-Pecos. Sida 21(3): 1663–1664. -Zomlefer, W.B. & Smith, G.L. 2002. Documented chromosome numbers 2002: 1. Chromosome number of Stenanthium (Liliales: Melanthiaceae) and its significance in the taxonomy of tribe Melanthieae. Sida 20(1): 221-226. -Zomlefer, W.B. & Smith, G.L. 2002. Documented chromosome numbers 2002: 1. Chromosome number of Stenanthium (Liliales: Melanthiaceae) and its significance in the taxonomy of tribe Melanthieae. Sida 20(1): 221-226. -Chinnappa, C. C. & J. G. Chmielewski. 1987. Documented plant chromosome numbers 1987: 1. Miscellaneous counts from western North America. Sida 12: 409–417. Toxicoscordion exaltatum 22* Toxicoscordion fontanum 22* Toxicoscordion fremontii 22* Toxicoscordion gramineum 22 Toxicoscordion micranthum 22 Toxicoscordion paniculatum 22 Toxicoscordion venenosum 22* Veratrum album 32* Veratrum californicum v. caudatum 32* Veratrum fimbriatum 32* Veratrum grandiflorum 32* Veratrum lobelianum 32* -McNeal, D.W. & Zomlefer, W.B. 2010. Documentation on the chromosome number for the California endemic, Toxicoscordion exaltatum (Liliales: Melanthiaceae). Madrono 57(3):180-183. -Preece, S.J. 1956. A cytotaxonomic study of the genus Zigadenus. PhD dissertation. State College of Washington, Pullman, WA. - Preece, S.J. 1956. A cytotaxonomic study of the genus Zigadenus. PhD dissertation. State College of Washington, Pullman, WA. -Miller, E.W. 1930. A preliminary note on the cytology of the Melanthoideae section of the Liliaceae. Proceedings of the University of Durham Philosophical Society 8:267-271. -Taylor, M.N. & Taylor, S. 1977. Chromosome numbers of vascular plants of British Columbia. Syesis 10:125-138. - Preece, S.J. 1956. A cytotaxonomic study of the genus Zigadenus. PhD dissertation. State College of Washington, Pullman, WA. - Preece, S.J. 1956. A cytotaxonomic study of the genus Zigadenus. PhD dissertation. State College of Washington, Pullman, WA. - Preece, S.J. 1956. A cytotaxonomic study of the genus Zigadenus. PhD dissertation. State College of Washington, Pullman, WA. -Pashuk, K. T. 1987. Chromosome numbers in species of subalpine belt of Chernogora (Ukrainian Carpatians). Bot. Zhurn. 72: 1069–1074. -Druskovic, B. & M. Lovka. 1995. IOPB chromosome data 9. Int. Organ. Pl. Biosyst. Newslett. (Zurich) 24: 15–19. -Fernandez Casas, J. & J. F. Piqueras. 1978. In Numeros cromosomicos para la flora Espanola. 1-44. Lagascalia 7: 191–216. - Cave, M. S. Chromosomes of California Liliaceae. Univ. Calif. Publ. Bot 57:1-58. - Cave, M. S. Chromosomes of California Liliaceae. Univ. Calif. Publ. Bot 57:1-58. -Probatova. 2004. Chromosome numbers of selected vascular plant species from Sakhalin, Moneron and the Kurile Islands. Biodivers. Biogeogr. Kuril Islands Sakhalin 1: 15–23. -Krogulevich, R. E. 1978. Kariologicheskij analiz vidov flory Vostochnogo Sajana. V Flora Pribajkal'ja. 19–48. Nauka, Novosibirsk. -Krogulevich, R. E. 1976. Rol poliploidii v genesise flory Putorana. In: L. I. Malyshev, ed. 1976 Flora Putorana: Materali k Poznaniiu Osobennostei Sostava i Genezisia Gornykh Subarkticheskikh Flor Sibir. Novosibirsk. -Krasnoborov, I. M., N. S. Krukova & V. V. Rubtsova. 1968. Nekotorye dekorativnye rastenija -Zapadnogo Sajana i ikh khromosomnyj sostav. Introdukcija Dekorat. Rastenij dla Cvetnikov i Gazonov Sibiri. Novosibirsk. K19881339 AF303687 n/a Forbes s.n. (UCBG84.0079) (GA) AF303682 n/a K1991203 AF303688 n/a AF303684 n/a AF303686 n/a AF303689 n/a K1999204 AF303683 n/a Pellicer s.n.(K)/K2010993 AF303674 n/a K196919705 AF303673 n/a HEL6248/ Forbes s.n. (UCBG540517) (GA) AF303676 n/a DQ517452 n/a Forbes s.n. (UCBG85.1381) (GA) Forbes s.n. (UCBG85.1498) (GA) K20102435 K19783034 DQ517452 n/a Veratrum longibracteatum 16* Veratrum maackii var. japonicum 16* Veratrum maackii var. maackii 16* Veratrum nigrum 16* Veratrum parviflorum 16 Veratrum schindleri 16 Veratrum stamineum 32* Veratrum virginicum 16 Veratrum viride 32* Veratrum woodii 16 1968: 30–34. -Kartashova, N. N., L. A. Malakhova, Koslova & N. A. Dubrova. 1974. Chisla chromosom u rjada polesnykh rastenij is prirodnykh populjacij flory Priob'ja. Biol. Biofis. Tomsk. 47–53. -Matsuura, H., Suto, T.1935. Contribution to the Idiogram study of phanerogamous plants I. Journal of the Faculty of Science, Hokkaido Imperial University. Ser. 5, Botany, 5(1): 33-75. -Lee, N. s. 1985. A cytotaxonomic study of Korean Veratrum species. Korean J. Pl. Taxon. 15: 155–161. -Takada, J. & H. Kawano. 1996. Distribution and variation of Veratrum maackii in Japan. J. Jap. Bot. 71: 11–28. -Lee, N. s. 1985. A cytotaxonomic study of Korean Veratrum species. Korean J. Pl. Taxon. 15: 155–161. -Rostovtseva, T. S., I. M. Krasnoborov & S. A. Krasnikova. 1981. Chisla khromosom nekotorych vidov flory Sibiri. Sb. Novye Dannye o Fitogeografii Sibiri. 215–220. -Malakhova, L. A. 1990. Kariologocheskij analiz prirodnykh populjacij redkich i ischezajushchikh rastenij na juge Tomskoj Oblasti. Bjulleten' Glavnogo Botanic|5eskogo Sada 155: 60–66. -Kiehn, M., E. Vitek, E. Hellmayr, J. Walter, J. Tschenett, C. Justin & M. Mann. 1991. Beiträge zur Flora von Österreich: Chromosomenzählungen. Verh. Zool.-Bot. Ges. Wien 128: 19–39. -Krasnoborov, I. M., T. S. Rostovtseva & S. A. Ligus. 1980. Chromosome numbers of some plant species of South Siberia and the Far East. Bot. Zhurn. 65 (5): 659–668. - Cave, M. S. Chromosomes of California Liliaceae. Univ. Calif. Publ. Bot 57:1-58. - Matsuura, H., Suto, T.1935. Contribution to the Idiogram study of phanerogamous plants I. Journal of the Faculty of Science, Hokkaido Imperial University. Ser. 5, Botany, 5(1): 33-75. -Cave, M. S. Chromosomes of California Liliaceae. Univ. Calif. Publ. Bot 57:158. -Kapoor, B. M. & C. Gerrais. 1982. Liste annotée de nombres chromosomiques de la flore vasculaire du nord- est de l'Amérique. III. Naturaliste Canad. 109: 91–101. -Smith, E. B. 1985. Chromosome Number Reports LXXXVIII. Taxon 34: 547– 551. K19885547/K20102431 HG475349 n/a K20102430 AF303678 AB017417 K20102425 AF303678 AB017417 K19977096/Pellicer s.n. (K)/K20101007 DQ517461 n/a Denslow & Denslow s.n. (BOON) (GA) K20102432 AF303681 n/a DQ517459 n/a K20102434 AB514905 AB040184 Poindexter s.n. (BOON) (GA) AF303679 n/a Pellicer s.n. (K) AF303675 n/a Zomlefer 2419 (ABG19920696) (GA) AF303677 n/a Zigadenus glaberrimus 54 -W. Zomlefer (in prep.) Walker s.n. (GA) AF303699 n/a Pellicer s.n. (K) n/a n/a K19953231 HG475366 HG475404 K19943438 HG475369 HG475403 Pellicer s.n. (K) HG475367 HG475399 K19962797/K20011796 HG475368 HG475400 Pellicer s.n. (K) HG475370 HG475405 Tribe Parideae Paris forrestii 10* Paris incompleta 10* Paris mairei 10* Paris japonica 40 Paris polyphylla 10* Paris quadrifolia 10* -Miyamoto, J., S. Kurita, G. Zhijian & L. Hen. 1992. C-Banding patterns in eighteen taxa of the genus Paris sensu Li, Liliaceae. Cytologia 57: 181–194. -Gu, Z. j., L. Wang & H. Li. 1992. Karyomorphological studies of some monocots in Dulongjiang area. Acta Bot. Yunnan. , Suppl. 5: 77–90. -Li, H. 1998. The Genus Paris (Trilliaceae). Science Press, Beijing. -Wang, L. 2004. Mapping of 18–26S rDNA loci in four species of the genus Paris by fluorescence in situ hybridization (FISH). Acta Phytotax. Sin. 42(5): 419–426 -Gagnidze, R. I., T. N. Gviniaschvili, M. G. Pataraia & L. D. Dzindzolia. 1985. Chromosome numbers in some high elevation species from the Big Caucasus. Bot. Zhurn. SSSR 70(12): 1698–1699. -Myakoshina, Y. A., E. O. Punina & A. V. Rodionov. 2002. Ag-NO staining of transcriptionally active nucleolus organizers in the karyotypes of the plants of the family Trilliaceae. Citologija 44(9): 894–895. -Punina. 2005. Karyosystematics and molecular phylogeny of Trilliaceae. Biol. Membrany 22: 249–257. -Myakoshina. 2006. Karyosystematic study of some Trilliaceae Cheval. In Thesis Diss. Cand. Biol. Sci. St. Petersburg. -Gu, Z. & H. Na. 1986. Karyotype studies in eight taxa of Paris. Acta Bot. Yunnan. 8: 313–318. Miyamoto, J., S. Kurita, G. Zhijian & L. Hen. 1992. C-Banding patterns in eighteen taxa of the genus Paris sensu Li, Liliaceae. Cytologia 57: 181–194. Gu, Z. j., L. Wang & H. Li. 1992. Karyomorphological studies of some monocots in Dulongjiang area. Acta Bot. Yunnan. , Suppl. 5: 77–90. -Pellicer, J., F. Fay & I.J. Leitch. 2010. The largest eukaryotic genome of them all? Bot. J. Linn. Soc. 164: 10-15. -Miyamoto, J., N. Ohmido & K. Fukui. 1999. Physical mapping of 18S rDNA by fluorescence in situ hybridization (FISH) in the three species of genus Paris L., Liliaceae. Cytologia 64: 175–180. -Mehra, P. N. & S. K. Sachdeva. 1979. Cytological observations on some East-Himalayan monocots. Cytologia 44: 233–240. -Chen, R. y., W. q. Song, X. l. Li, M. x. Li, G. l. Liang & C. b. Chen. 2003. Chromosome Atlas of Major Economic Plants Genome in China, Vol. 3, Chromosome Atlas of Garden Flowering Plants in China. Science Press, Beijing. -Punina. 2005. Karyosystematics and molecular phylogeny of Trilliaceae. Paris tetraphylla 10 Paris thibetica 10 * Paris thibetica var. thibetica 10* Paris verticillata 10* Trillium camschatcense 10* Trillium catesbaei 10 Trillium cernuum 10* Biol. Membrany 22: 249–257. -Krasnikov, A. A. 1991. Chromosome numbers in some species of vascular plants from Novosibirsk region. Bot. Žhurn. (Moscow & Leningrad) 76: 476– 479. -Punina, E. O., Y. A. Myakoshina, A. M. Efimov & A. V. Rodionov. 2000. Chromosome map of Trilliaceae plants: heterochromatin nucleotide composition and mapping of 18S–26S rRNA genes in Paris quadrifolia L. Genetika 36(5): 673–677. -Miyamoto, J., S. Kurita & K. Fukui. 1991. Image analysis of C-banding patterns in two herbs: Paris tetraphylla A. Gray and Paris verticillata M. v Bieb. (Liliaceae). Jap. J. Genet. 66: 335–345. -Wang, L. & A. Uchino. 1998. Cytogenetic structure and breeding system of natural populations of Paris tetraphylla A. Gray. Cytologia 63: 87–97. -Miyamoto, J., N. Ohmido & K. Fukui. 1999. Physical mapping of 18S rDNA by fluorescence in situ hybridization (FISH) in the three species of genus Paris L., Liliaceae. Cytologia 64: 175–180. -Li, H. 1998. The Genus Paris (Trilliaceae). Science Press, Beijing. -Gu, Z. & H. Na. 1986. Karyotype studies in eight taxa of Paris. Acta Bot. Yunnan. 8: 313–318. Miyamoto, J., S. Kurita, G. Zhijian & L. Hen. 1992. C-Banding patterns in eighteen taxa of the genus Paris sensu Li, Liliaceae. Cytologia 57: 181–194. -Li, H. 1998. The Genus Paris (Trilliaceae). Science Press, Beijing. -Miyamoto, J., S. Kurita & K. Fukui. 1991. Image analysis of C-banding patterns in two herbs: Paris tetraphylla A. Gray and Paris verticillata M. v Bieb. (Liliaceae). Jap. J. Genet. 66: 335–345. -Li, H. 1998. The Genus Paris (Trilliaceae). Science Press, Beijing. -Probatova, N. S., A. P. Sokolovskaya & E. G. Rudyka. 1989. Chromosome numbers in some species of vascular plants from Kunashir Island (the Kuril Islands). Bot. Žhurn. (Moscow & Leningrad) 74: 1675–1678. -Rudyka, E. G. 1998. Chromosome numbers of the monocotyledonous plants of the flora of Russian Far East. Pages 135–137 in Rasteniya v Mussonnom Klimate. Vladivostok. -Hill, L. M. 1995. IOPB chromosome data 10. Int. Organ. Pl. Biosyst. Newslett. (Zurich) 25: 8–9. -Darlington, C.D. & Shaw, G.W. 1959. Parallel polymorphism in the heterochromatin of Trillium species. Heredity 13: 89-121. Pellicer s.n. (K) n/a n/a K19821796 HG475371 HG475402 K19821797 HG475372 HG475401 Pellicer s.n. (K) n/a n/a K1995687 DQ404265 AB017379 K20003247 n/a n/a K195986503 HG475384 HG475425 Trillium chloropetalum 10* Trillium cuneatum 10 Trillium decipiens 10 Trillium discolor 10 Trillium erectum 10* Trillium erectum x flexipes 10* Trillium flexipes 10* Trillium govanianum 20* Trillium grandiflorum 10* Trillium kurabayashii 10* Trillium lancifolium 10 -Dyer, A. F. 1964. Heterochromatin in American and Japanese species of Trillium. Fusion chromocentres and the distribution of H-segments. Cytologia 29: 155-170. -Dyer, A. F. 1964. Heterochromatin in American and Japanese species of Trillium. Fusion chromocentres and the distribution of H-segments. Cytologia 29: 155-170. -Serota, C. A., Smith, B. W. 1967. The cyto-ecology of four species of Trillium (Liliaceae) from western North Carolina. Amer. J. Bot. 54: 159-181. -See: Flora of North America North of Mexico Vol. 26, Magnoliophyta: Liliidae: Liliales and Orchidales. Oxford University Press. New York and Oxford. (http://efloras.org/volume_page.aspx?volume_id=1026&flora_id=1, accessed 07/2013. -See: Flora of North America North of Mexico Vol. 26, Magnoliophyta: Liliidae: Liliales and Orchidales. Oxford University Press. New York and Oxford. (http://efloras.org/volume_page.aspx?volume_id=1026&flora_id=1, accessed 07/2013. -Fukuda, I. 1987. Chromosome compositions of natural populations in Trillium erectum. Sci. Rep. Tokyo Woman's Christian Univ. 76–79: 943–961. -Chinnappa, C. C. & J. K. Morton. 1978. Heterochromatic banding patterns in two species of Trillium. Canad. J. Genet. Cytol. 20: 475–481. -See: Flora of North America North of Mexico Vol. 26, Magnoliophyta: Liliidae: Liliales and Orchidales. Oxford University Press. New York and Oxford. (http://efloras.org/volume_page.aspx?volume_id=1026&flora_id=1, accessed 07/2013. -Chatterjee, A., S. Ghosh & S. C. Roy. 1989. A cytological survey of eastern Himalayan plants III. Cell Chromosome Research. 12: 22–29. -Wcislo, H. 1987. Chromosome numbers of certain Canadian plants. Acta Biol. Cracov., Ser. Bot. 29: 19–30. -Chinnappa, C. C. 1982. Cytology of some variegated forms of Trillium grandiflorum (Liliaceae). Caryologia 35: 23–22. -Fukuda, I. & W. F. Grant. 1980. Chromosome variation and evolution in Trillium grandiflorum. Canad. J. Genet. Cytol. 22: 81–91. -Baiely, P. C. 1951. A study of the chromosome morphology of some species of Trillium. Bull. Torrey Bot. Cl. 78: 324-330. K1978977 HG475376 HG475413 Zomlefer 2420 (GA) HG475386 AB017384 Zomlefer 2417 (GA) HG475374 HG475418 Hill 35072 (ILL) HG475375 HG475419 K20091917 HG475391 HG475423 K19884343 HG475393 HG475427 K1991513 HG475392 HG475424 K19835858 HG475382 HG475408 K1980517 HG475394 HG475409 Pellicer s.n. (K) HG475377 HG475421 K19962636 HG475381 HG475420 Trillium luteum 10 * Trillium maculatum 10 Trillium nivale 10 Trillium ovatum 10* Trillium parviflorum 10 * Trillium pusillum var. pusillum 10 * Trillium recurvatum 10 * Pseudotrillium rivale 10* Trillium sessile 10* Trillium sulcatum 10 Trillium taiwanense 20 * Trillium tschonoskii 20* -Darlington, C.D. & Shaw, G.W. 1959. Parallel polymorphism in the heterochromatin of Trillium species. Heredity 13: 89-121. -Dyer, A. F. 1964. Heterochromatin in American and Japanese species of Trillium. Fusion chromocentres and the distribution of H-segments. Cytologia 29: 155-170. -Hill, L. M. 1995. IOPB chromosome data 9. Int. Organ. Pl. Biosyst. Newslett. (Zurich) 24: 19–20. -Darlington, C.D. & Shaw, G.W. 1959. Parallel polymorphism in the heterochromatin of Trillium species. Heredity 13: 89-121. - Serota, C. A., Smith, B. W. 1967. The cyto-ecology of four species of Trillium (Liliaceae) from western North Carolina. Amer. J. Bot. 54: 159-181. - Baldwin, J. T.; Bernice, M.; Mikula, S.; Mikula, B. 1949. Chromosomes of Trillium pusillum Fern. Rhodora 51: 368 -Darlington, C.D. & La Cour, L. F. 1940. Journal of Genetics 40: 185. -Punina. 2005. Karyosystematics and molecular phylogeny of Trilliaceae. Biol. Membrany 22: 249–257. -Warmke, H. E. 1937. Cytology of the Pacific Coast Trilliums. American Journal of Botany 24:276-386 -Utech, F. H. 1980. Chromosome atlas of the vascular plants of western Pennsylvania. I. Ann. Carnegie Mus. 49: 265–305. -Hill, L. M. 1995. IOPB chromosome data 9. Int. Organ. Pl. Biosyst. Newslett. (Zurich) 24: 19–20. -Darlington, C.D. & Shaw, G.W. 1959. Parallel polymorphism in the heterochromatin of Trillium species. Heredity 13: 89-121. -Dyer, A. F. 1964. Heterochromatin in American and Japanese species of Trillium. Fusion chromocentres and the distribution of H-segments. Cytologia 29: 155-170. -Patrick, T. S. 1984. Trillium sulcatum (Liliaceae), a new species of the southern Appalachians. Brittonia 36(1): 26–36. -Wang, S. f. 1989. Karyotype uniformity of Paris and Trillium tschonoskii. Acta Bot. Yunnan. 11: 75–79. -Myakoshina, Y. A., E. O. Punina & A. V. Rodionov. 2002. Ag-NO staining of transcriptionally active nucleolus organizers in the karyotypes of the plants of the family Trilliaceae. Citologija 44(9): 894–895. -Punina. 2005. Karyosystematics and molecular phylogeny of Trilliaceae. Biol. Membrany 22: 249–257. K19911817 HG475387 HG475414 Judd 8350 FLAS (GA) HG475388 HG475412 K19884374 n/a n/a Forbes s.n. (UCBG76.0117), (GA)/ K195986502 HG475395 HG475416 K1989194 HG475378 HG475415 K1991855 n/a n/a K19873785 HG475390 HG475422 K1991518 HG475380 HG475417 K1991853 HG475379 HG475410 HG475385 HG475426 HG475373 HG475406 DQ404269 AB017411 Poindexter 11-114 (BOON) (GA)/ RHS50805 K20102428 K19911709 Trillium undulatum 10 Trillium viride 10 -Löve, A. & D. Löve. 1982. IOPB chromosome number reports LXXVII. Taxon 31: 766–768. -Plante, S. 1995. IOPB chromosome data 10. Int. Organ. Pl. Biosyst. Newslett. (Zurich) 25: 9–10. -See: Flora of North America North of Mexico Vol. 26, Magnoliophyta: Liliidae: Liliales and Orchidales. Oxford University Press. New York and Oxford. (http://efloras.org/volume_page.aspx?volume_id=1026&flora_id=1, accessed 07/2013. Poindexter 09-110 (BOON) (GA) HG475383 HG475407 Hill 34571 (ILL) HG475389 HG475411 Zomlefer 2422 (ABG20100685) (GA) HG475364 HG475398 K20003137/K20101013 HG475365 HG475397 Tribe Xerophylleae Xerophyllum asphodeloides 30 Xerophyllum tenax 30* -Miller, E. W. 1930. A preliminary note on the cytology of the Melanthioideae section of the Liliaceae. Proc. Univ. Durham Philos. Soc. 8: 267-271. 1930. -Chambers, K. L., D. Green, S. Potampa & L. McMahan. 1998. IOPB chromosome data 13. Newslett. Int. Organ. Pl. Biosyst. (Oslo) 29: 18–22. Flora of North America Editorial Committee, Eds. 2002. *New chromosome numbers obtained in the present study using the same accessions as for GS assessments. Supporting Information Table S2: Model fitness scores obtained with ChromEvol. MODELS CONST_RATE_NO_DUPL (M0) CONST_RATE (M1) CONST_RATE_DEMI (M2) CONST_RATE_DEMI_EST (M3) LINEAR_RATE (M4) LINEAR_RATE_DEMI (M4a) LINEAR_RATE_DEMI_EST (M4b) LINEAR_RATE_NO_DUPL (M5) Log-likelihood (LRT) -279.7 -87.82 -82.57 -82.45 -87.15 -82.74 -82.68 -256.8 Akaike information criterion (AIC)* 563.3 181.6 171.1 172.7 184.3 175.5 176.6 521.6 *As recommended in Mayrose et al. (2010), AIC has been used to determine the model that best fits to the dataset.