Prokaryotes: Bacteria & Archaea Name: Bacteria, which outnumber all eukaryotes combined, differ from eukaryotes in at least seven ways. Membrane-Bound Organelles: Bacteria are prokaryotes. Unlike eukaryotes, prokaryotes lack a cell nucleus and all other membrane-bound organelles. Prokaryotes do contain ribosomes, granules of stored nutrients, enzymes, and certain internal structures, however. Cell Size: Most bacterial cells are about 1 µm in diameter; most eukaryotic cells are more than 10 times that size. Multicellularity: All bacteria are single cells. Some eukaryotes are single-celled, but most are multicellular. Chromosomes: Bacterial chromosomes consist of a single circular piece of DNA. Eukaryotic chromosomes are linear pieces of DNA that are associated with proteins. Prokaryotes also have plasmids, small extra loops of DNA. Reproduction: Bacteria reproduce by binary fission, a process in which one cell pinches into two identical cells. In eukaryotes, however, microtubules pull chromosomes to opposite poles of the cell during mitosis. Afterward, the cytoplasm of the eukaryotic cell divides in half, forming two cells. Flagella and Pili: Bacterial flagella are simple structures that spin like a corkscrew to move the cell. Eukaryotic flagella are more-complex structures made of microtubules that whip back and forth rather than spin. Some bacteria also have short, thin, protein tubes called pili that attach to surfaces or to other cells. Metabolic Diversity: Bacteria have diverse metabolic abilities. For example, some bacteria get their energy from sunlight through photosynthesis. Others get energy from inorganic molecules that contain sulfur or nitrogen. Most bacteria get nutrients and energy by feeding on other organisms. In the space provided, write the letter of the phrase that best completes the statement. 1. Write 3 things that describe the characteristics of prokaryotes. You can copy any 3 pieces of information from above. To the left of the number, write E if the characteristic describes only eukaryotes, B if the characteristic describes only bacteria, or EB if the characteristic describes both eukaryotes and bacteria. 2. B 3. B 4. E 5. EB 6. B 7. E 8. B 9. EB 10. E 11. EB 12. EB 13. EB 14. E 15. E 16. B 17. E 18. B 19. EB 20. B 21. EB 22. B 23. B 24. EB 2. reproduce by binary fission 3. lack a cell nucleus 4. chromosomes are linear pieces of DNA 5. have pili for attaching to surfaces and other cells 6. have plasmids 7. divide by mitosis 8. most cells are about 1 µm in diameter 9. single cells 10. cytoplasm contains membrane-bound organelles 11. simple flagella spin like a corkscrew 12. are about 10 µm in diameter 13. flagella are complex structures made of microtubules 14. most are multicellular 15. during cell division, microtubules pull chromosomes to opposite poles 16. cytoplasm lacks organelles 17. lack plasmids 18. some get energy from inorganic molecules that contain sulfur or nitrogen 19. two identical cells form when cytoplasm pinches in half 20. chromosomes consist of a single circular piece of DNA 21. have ribosomes 22. have a single chromosome called a nucleoid 23. have a cell wall made of peptidoglycan 24. have enzymes for performing metabolic activities 25. How do the sizes compare between virus, bacteria, and eukaryotic cells? Virus < bacteria < eukaryotic cells 26. (Underline)Escherichia coli are [Gram-negative / Gram-positive] eubacteria. 27. (Underline) [Conjugation / Transduction] is a process in which two bacteria exchange genetic material. 28. (Underline)Escherichia coli have [pili and flagella / only flagella]. 29. (Underline)Many bacteria move with [flagella / pili]. 30. Complete the chart. Define Photoautotrophic Another way of saying photosynthesis. Using sunlight to create organic compounds. Chemoautotrophic Obtain their own energy by oxidizing inorganics (like hydrogen sulfide) Heterotrophic Technically, obtain the carbon necessary for energy production from organic molecules like glucose, have to consume to get access. Eukaryotic, prokaryotic, or both Both (plants & many bacteria) Prokaryotic Both In the space provided, write the name of the metabolic group—photoautotrophic, chemoautotrophic, or heterotrophic—to which each of the following types of bacteria belongs. ___ photoautotrophic ____ 31. Cyanobacteria ____ photoautotrophic ____ 32. purple sulfur bacteria ____ chemoautotrophic ____ 33. bacteria that form their own proteins from hydrogen-rich chemicals like H2S _____ photoautotrophic ___ 34. Anabaena _____ heterotrophic ___ 35. Rhizobium 36. The two domains of prokaryotes are called ___eubacteria (Monera)__ and ___archaebacteria___. 37. Bacteria often have small extra loops of DNA called ___Plamsids___. 38. The cell wall of bacteria contains a protein-carbohydrate compound called ____peptidoglycan or glycoprotein___. 39. Gram-negative bacteria are more resistant to medicines because they have a(n) ___thicker cell wall _________. 40. Some bacteria can survive harsh conditions by forming thick-walled __endospore______. 41. List and describe 1 beneficial bacteria. Nitrifying bacteria – metabolize inert atmospheric nitrogen into usable forms Flora – help hosts metabolize certain substances (E.coli help us make vitamins B & K) 42. List and describe 1 harmful bacteria (not anthrax). Staphylococcus – food poisoning See pg 447 of blue book or pg 351 in grayple (grey-purple) book for list 43. Describe anthrax and explain the concern with it. Anthrax produces lethal poisons when active but can be dormant as very tough spores. Because of its resilience and other characteristics (odorless), it has been used as a source of bioterrorism.