Supplementary Data
99
OO
71
*
*
*
170
Supplementary Fig. 1
GC/MS spectrum of the reaction product obtained from the
conversion of 13-hydroxy-9(Z)-octadecenoic acid to reaction product by whole Y. lipolytica
cells. The reaction product was identified as δ-decalactone.
a
120
Relative activity (%)
100
80
60
40
20
0
15
20
25
30
35
40
45
o
Temperature ( C)
b
120
Relative activity (%)
100
80
60
40
20
0
5.0
5.5
6.0
6.5
7.0
7.5
8.0
8.5
9.0
pH
Supplementary Fig. 2
Effects of temperature and pH on the conversion of 13-hydroxy-9(Z)-
octadecenoic acid to δ-decalactone by whole Y. lipolytica cells. a Effect of temperature. The
reactions were performed in 50 mM Tris/HCl buffer (pH 7.0) containing 3.4 g yeast nitrogen
base l–1, 7 g cells l−1, 0.5 g 13-hydroxy-9(Z)-octadecenoic acid l−1, and 0.05% (v/v) Tween 80
for 3 h. b Effect of pH. The reactions were performed in 50 mM citrate/phosphate buffer (filled
circle, pH 5.5−7) buffer and 50 mM Tris/HCl buffer (empty circle, pH 7.0−8.5) containing 3.4
g yeast nitrogen base l–1, 7 g cells l−1, 0.5 g 13-hydroxy-9(Z)-octadecenoic acid l−1, and 0.05%
(v/v) Tween 80 for 3 h. Data represent the means of three separate experiments and error bars
represent standard deviation.
1.4
-1
-Decalactone (g l )
1.2
1.0
0.8
0.6
0.4
0.2
0.0
0
1
2
3
4
5
-1
Linoleic acid concentration (g l )
Supplementary Fig. 3
Effect of the concentration of linoleic acid on the production of δ-
decalactone from 13-hydroxy-9(Z)-octadecenoic acid by whole Y. lipolytica cells. The
reactions were performed by varying the concentration of linoleic acid from 0 to 4 g l–1 in 50
mM Tris/HCl buffer (pH 7.5) containing 3.4 g yeast nitrogen base l–1, 25 g cells l–1, 7.5 g 13hydroxy-9(Z)-octadecenoic acid l–1, and 0.05% (v/v) Tween 80 at 30 °C in a 100 ml baffled
flask containing 10 ml reaction mixture with agitation at 200 rpm for 12 h. Data represent the
means of three separate experiments and error bars represent standard deviation.