Supplementary Table 1 The predictive values for the onset of KD with single SNP and multi-locus models
Sensitivity, %
Specificity, %
PPV, %
NPV, %
rs1801274 (FCGR2A)
53.6
55.1
34.4
76.8
rs2857151 (HLA)
58.1
49.7
33.7
73.0
rs2254546 (BLK)
68.4
40.9
33.7
74.7
rs1801274+ rs2857151
32.1
77.3
38.3
72.2
rs113420705+ rs2254546
36.0
73.5
37.4
72.3
12.6
93.7
46.9
70.9
Single SNP (Gene)*
2-locus models*,#
3-locus models*,&
rs113420705 + rs2857151+ rs4813003
PPV: positive predictive value; NPV: negative predictive value.
*
The single SNP and multi-locus models observed significant effect on KD in regression analyses.
#
The frequency comparison of subjects with high-risk genotypes at both loci (See Table 3) between 358 patients with KD and 815 controls.
&
The frequency comparison of subjects with high-risk genotypes at all three loci (See Table 3) between 358 patients with KD and 815 controls.
1
Supplementary Table 2 Conditional multi-variable logistic regression analyses for the associations of the SNPs with CALs
SNP (Gene)
Allele
Genotype
High-risk
Low-risk
OR (95% CI)
P valuea (α’ = 0.007)
rs1801274 (FCGR2A)
A/G
AA
AG/GG
1.915 (0.986,3.722)
0.055
rs113420705 (CASP3)
G/A
AA/AG
GG
1.574 (0.809,3.063)
0.182
rs2857151 (HLA)
A/G
GG
AG/AA
1.702 (0.823,3.520)
0.152
rs2254546 (BLK)
A/G
GG
AG/AA
1.343 (0.706,2.555)
0.368
rs4813003 (CD40)
C/T
CC
CT/TT
1.408 (0.740,2.680)
0.297
The risk alleles were underlined.
a
Multiple regression analysis in 46 KD patients with CALs and 312 KD patients without CALs. The P values were adjusted for gender and age. The significant level
was corrected with the formula of α’ = α/5 = 0.01 (5 tests, 5 variables × 1 test/variable) according to the Bonferroni method.
2
Supplementary Table 3 Conditional multi-variable logistic regression analyses for the associations of the SNPs with IVIG unresponsiveness
SNP (Gene)
Allele
Genotype
High-risk
Low-risk
OR (95% CI)
P valuea (α’ = 0.007)
rs1801274 (FCGR2A)
A/G
AA
AG/GG
1.232 (0.666,2.280)
0.506
rs113420705 (CASP3)
G/A
AA/AG
GG
1.083 (0.583,2.012)
0.802
rs2857151 (HLA)
A/G
GG
AG/AA
1.340 (0.675,2.657)
0.403
rs2254546 (BLK)
A/G
GG
AG/AA
1.051 (0.568,1.946)
0.874
rs4813003 (CD40)
C/T
CC
CT/TT
1.052 (0.574,1.926)
0.870
The risk alleles were underlined.
a
Multiple regression analysis in 51 KD patients with IVIG unresponsiveness and 307 KD patients without IVIG unresponsiveness. The P values were adjusted for
gender and age. The significant level was corrected with the formula of α’ = α/5 = 0.01 (5 tests, 5 variables × 1 test/variable) according to the Bonferroni method.
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Supplementary Table 4 Association of the SNPs with general characteristics in patients with KD
High risk groupa (n)
Low risk groupb (n)
P valuec
rs1801274*
1.57 ± 1.86 (192)
1.63 ± 1.93 (166)
0.765
rs2857151*
1.62 ± 1.80 (208)
1.57 ± 1.96 (150)
0.803
rs2254546*
1.53 ± 1.78 (245)
1.72 ± 2.01 (113)
0.369
Male (%)
73 (63.5)
149 (61.3)
0.694
Female (%)
42 (36.5)
94 (38.7)
Male (%)
84 (65.1)
138 (60.3)
Female (%)
45 (34.9)
91 (39.7)
Male (%)
29 (64.4)
193(61.7)
Female (%)
16 (35.6)
120 (38.3)
General characteristics
Age at onset (years, mean ± SD)
Gender
rs1801274+ rs2857151*
rs113420705+ rs2254546*
0.364
rs113420705 + rs2857151+ rs4813003*
a
Subjects with high-risk genotypes shown in Table 3 at all loci.
4
0.719
b
Subjects with low-risk genotype shown in Table 3 at any locus.
c Student’s
t test for the comparison of age at onset, the significant level was corrected with the formula of α’ = α/3 = 0.017 (3 tests, 3 SNPs × 1 test/SNP) according
to the Bonferroni method. Chi-Squared test for the comparison of gender, the significant level was corrected with the formula of α’ = α/3 = 0.017 (3 tests, 3
multi-locus models × 1 test/ multi-locus models) according to the Bonferroni method.
*
The single SNP and multi-locus models observed significant effect on Kawasaki disease in regression analyses.
5