Supplementary information for
Hartman et al.
Abiotic stress QTL in lettuce crop–wild hybrids: comparing greenhouse and field
experiments
Table S1. Number of replicates per line used in the greenhouse competition and field
experiments.
Figure S1. Schematic representation of experimental design.
Figure S2. Water capacity of empty pots during the stress period of the
Drought/Recovery experiment.
Figure S2b. Electric conductivity of empty pots during the stress period.
Figure S3. Data distributions of input data for the QTL analyses.
Appendix S1. Environmental conditions during the greenhouse experiments.
Supplementary information for Hartman et al.
Abiotic stress QTL in lettuce
Table S1. Number of replicates per line used in the greenhouse competition and field experiments.
RIL
Control
Greenhouse
Nutrient
limitation Drought
Field
Salt
100mM
Control
Drought
Salt
100mM
L. sativa cv. Salinas
L. serriola
UC96US23
17
17
17
17
17
14
10
17
17
17
17
13
10
12
114
17
17
17
17
17
14
14
115
17
0
17
17
17
14
13
116
17
17
17
17
15
14
13
119
12
15
13
11
11
10
10
120
17
17
17
17
16
14
14
121
7
17
7
7
17
13
16
122
17
17
17
17
16
16
16
124
14
17
16
15
14
11
7
125
14
17
16
17
17
15
15
126
2
17
3
3
14
12
16
127
17
7
17
17
17
16
13
128
13
8
13
13
9
7
11
129
17
17
17
17
16
15
14
130
17
17
17
17
16
13
12
131
14
17
14
17
7
7
7
132
9
17
10
10
17
15
16
133
10
16
10
10
12
9
8
134
17
17
17
17
16
14
16
135
17
16
17
17
16
14
15
136
15
17
15
16
9
10
14
139
17
17
17
17
17
13
11
140
9
17
10
11
14
13
8
141
9
17
9
9
9
9
6
142
12
17
12
13
17
14
17
143
17
17
15
16
17
15
12
144
17
17
17
17
15
14
16
145
3
13
3
4
10
7
8
146
17
17
17
17
15
16
15
148
17
17
17
17
9
8
9
149
17
17
17
17
17
14
15
150
13
17
14
13
17
11
17
151
17
17
17
17
16
14
14
152
17
17
17
17
16
15
16
154
17
17
17
17
15
12
14
155
16
17
14
16
15
15
13
156
17
17
17
17
15
14
13
158
11
5
12
12
9
7
5
159
14
17
15
15
17
12
14
160
17
17
17
17
15
16
16
162
17
17
17
17
16
14
15
163
10
17
9
9
14
14
11
164
17
17
17
17
17
15
16
166
17
17
17
17
16
16
14
167
13
17
13
13
17
14
13
169
13
14
13
13
10
0
0
170
17
17
17
17
17
15
13
2
Supplementary information for Hartman et al.
Abiotic stress QTL in lettuce
Table S1. Continued
Greenhouse
Nutrient
limitation Drought
Field
Salt
100mM
Control
Drought
Salt
100mM
RIL
Control
171
17
17
17
17
17
13
14
172
15
17
15
16
17
15
15
173
9
17
8
8
6
5
4
175
17
11
17
17
12
14
13
176
17
17
17
17
16
13
12
177
17
17
17
17
16
14
11
178
17
17
17
17
14
13
10
179
12
15
12
11
13
14
14
180
17
17
17
17
17
16
11
181
8
4
8
11
14
8
10
182
17
17
17
17
17
16
16
183
17
17
17
17
12
11
12
186
17
17
17
17
15
15
15
187
13
15
13
14
15
14
13
190
17
17
17
17
16
15
13
192
15
13
17
17
17
17
14
193
17
17
17
17
16
15
12
194
17
17
17
17
16
13
15
195
17
17
17
17
16
14
12
196
17
17
17
17
13
14
15
197
17
16
17
17
15
14
12
199
10
14
16
16
16
11
13
201
17
17
17
17
16
16
14
202
17
10
17
17
16
16
16
203
17
17
17
17
13
11
14
204
17
17
17
17
16
14
15
205
13
17
17
17
15
14
11
206
15
17
15
15
16
15
17
207
17
14
17
17
16
15
16
209
17
17
17
17
17
15
14
211
17
17
17
17
15
15
10
212
17
17
17
17
17
14
13
213
17
11
17
17
15
14
12
214
12
17
12
12
9
10
9
215
14
15
14
14
13
12
9
217
14
12
14
15
15
13
16
218
12
17
12
12
17
16
16
219
17
17
17
17
16
11
14
220
16
17
17
16
17
14
14
221
15
17
15
15
15
13
15
222
17
17
17
17
16
11
14
223
13
17
11
12
13
14
14
227
12
14
13
13
16
14
13
228
14
9
15
12
10
11
11
14.8
15.7
15.0
15.1
14.7
13.0
12.8
average n treatment
average n
experiment
15.2
13.5
3
Supplementary information for Hartman et al.
Abiotic stress QTL in lettuce
Figure S1. Schematic representation of experimental design. Blue = germination period, green = establishment period, orange = stress period, purple = biomass collection,
and dark blue = rewater period. 1 = drought treatment collected, 2 = control and recovery treatment collected.
Week
Salt/ Nutrient
limitation
Drought/
Recovery
Greenhouse
competition
Field stress
1
2
3
4
5
6
7
1
4
2
Supplementary information for Hartman et al.
Abiotic stress QTL in lettuce
Figure S2a. Water capacity of empty pots during the stress period of the Drought/Recovery
experiment.
1200
1100
900
Control
800
Recovery
Drought
700
600
500
400
Start
wk1
wk2
wk3
Drought
biomass
collection
rewater
End
Time points
1.8
16
1.6
1.4
1.2
1
0.8
0.6
12
16
16 10
14
14 8
12
12 6
10
10 4
8
8
6
1
3
4
Week
0.4 0.2
0.2
2
2
0
1
0
1
2
3
2 Week
Week
5
3
0
2
6
0
4
2
0
Electric conductivity
Electric(Sm-1)
conductivity (Sm-1)
1.8
1.4
1.8
1.2
1.6
1
1.4
0.8
1.2
0.6
1
0.4
0.8
0.2
0.6
0
0.4
Electric conductivity (Sm-1)
Electric conductivity
Electric(Sm-1)
conductivity (Sm-1)
Figure S2b.
Electric conductivity of empty pots during the stress period. Control and nutrient
1.6
14
limitation treatments are on the left axis and the salt treatments are on the right axis.
Electric conductivity (Sm-1)
Weight (g)
1000
Control
Nutrient limitation
Control
Nutrient limitation
Control
Salt 100mM
Nutrient limitation
Salt 300mM
Salt 100mM
Salt 300mM
Salt 100mM
Salt 300mM
Supplementary information for Hartman et al.
Abiotic stress QTL in lettuce
Figure S3. Data distributions of input data for the QTL analyses. Shown are the
transformed data as used as input for QTL cartographer in histograms of 10 bars for
all treatments as presented in Table 1-3 and Figures 1 and 2. Intervals between bars
are given and are different for every treatments.
Dry weight of aboveground biomass of the Greenhouse salt & nutrient experiment: a)
control treatment; b) Added salt of 100mM; c) Nutrient limitation (no addition).
Dry weight of aboveground biomass of the Greenhouse Drought experiment: d)
control treatment; e) 23 days of drought; f) 4 days of recovery after drought.
Dry weight of aboveground biomass of the Greenhouse Competition experiment: g)
increased competition (high density) only; h) increased competition and nutrient
limitation; i) increased competition and added salt 100mM; j) increased competition
and 23 days of drought.
Dry weight of aboveground biomass of the Field experiment: k) general field
conditions only; l) general field conditions and Salt added 100mM; m) general field
conditions and Drought.
Proportion Dry weight of fresh biomass of the Greenhouse salt & nutrient experiment:
n) control treatment; o) Added salt of 100mM; p) Nutrient limitation (no addition).
Proportion Dry weight of fresh biomass of the Greenhouse Drought experiment: q)
control treatment; r) 23 days of drought; s) 4 days of recovery after drought.
6
Supplementary information for Hartman et al.
Abiotic stress QTL in lettuce
a) 15
b) 12
Control for Salt & Nutrient experiment
Salt 100mM (greenhouse)
12
# RILs within interval
# RILs within interval
9
9
6
3
0
6
3
0
Dry weigh above ground biomass in log(grams+1). Interval = 0.032
c) 12
Dry weigh above ground biomass in log(grams+1). Interval = 0.0293
d) 15
Nutrient limitation (greenhouse)
Control for Drought experiment
12
# RILs within interval
# RILs within interval
9
6
3
0
9
6
3
0
Dry weigh above ground biomass in log(grams+1). Interval = 0.026
e) 15
Dry weigh above ground biomass in log(grams+1). Interval = 0.034
f)
Drought (greenhouse)
Revovery after Drought (greenhouse)
12
# RILs within interval
12
# RILs within interval
15
9
6
3
0
9
6
3
0
Dry weigh above ground biomass in log(grams+1). Interval = 0.0099
Fig S3.
7
Dry weigh above ground biomass in log(grams+1). Interval = 0.0095
Supplementary information for Hartman et al.
Abiotic stress QTL in lettuce
h) 18
Competition only
16
16
14
14
12
12
# RILs within interval
# RILs within interval
g) 18
10
8
6
4
10
8
6
4
2
2
0
0
Dry weigh above ground biomass in log(grams+1). Interval = 0.0206
Dry weigh above ground biomass in log(grams+1). Interval = 0.0346
20
j)
Competition and Salt (100mM)
18
18
16
16
14
14
12
# RILs within interval
# RILs within interval
i)
Competition and Nutrient limitation
12
10
8
6
Competition and Drought
10
8
6
4
4
2
2
0
0
0.0640.09
< 0.12
< 0.15
< 0.17
< 0.20
< 0.23
< 0.26
< 0.28
< 0.31
< 0.34
< 0.366
Dry weigh above ground biomass in log(grams+1). Interval = 0.0274
l)
Field experiment, field only
22
18
16
16
14
12
10
8
6
4
14
12
10
8
6
4
2
2
0
0
Dry weigh above ground biomass in log(grams+1). Interval = 0.043
m) 2220
Salt 100mM (field)
20
18
# RILs within interval
# RILs within interval
k) 2220
Dry weigh above ground biomass in log(grams+1). Interval = 0.0143
Dry weigh above ground biomass in log(grams+1). Interval = 0.045
Drought (field)
18
# RILs within interval
16
14
12
10
8
6
4
2
0
Dry weigh above ground biomass in log(grams+1). Interval = 0.044
Fig S3 (continued).
8
Supplementary information for Hartman et al.
Abiotic stress QTL in lettuce
n) 15
o) 12
Control for Salt & Nutrient experiment
Salt 100mM (greenhouse)
12
# RILs within interval
# RILs within interval
9
9
6
3
6
3
0
0
Proportion Dry weigh above ground biomass Arcsin transformed.
Interval = 0.004
Proportion Dry weigh above ground biomass Arcsin transformed.
Interval = 0.004
p) 15
q) 18
Nutrient limitation (greenhouse)
Control for Drought experiment
15
12
# RILs within interval
# RILs within interval
12
9
6
3
0
9
6
3
0
Proportion Dry weigh above ground biomass Arcsin transformed.
Interval = 0.011
r)
18
Proportion Dry weigh above ground biomass Arcsin transformed.
Interval = 0.0105
s) 15
Drought (greenhouse)
15
Revovery after Drought (greenhouse)
12
# RILs within interval
# RILs within interval
12
9
6
3
0
9
6
3
0
Proportion Dry weigh above ground biomass Arcsin transformed.
Interval = 0.012
Proportion Dry weigh above ground biomass Arcsin transformed.
Interval = 0.007
Fig S3 (continued).
9
Supplementary information for Hartman et al.
Abiotic stress QTL in lettuce
Appendix S1. Environmental conditions during the greenhouse experiments.
For both the Drought/recovery experiment and the Salt/nutrient limitation experiment,
treatment conditions were stable throughout the stress period (See Supplementary material
Figure S2). In the Drought experiment temperatures ranged from 17.9°C to 22.5°C, with an
average temperature of 19.6°C and relative humidity of 77.8%. In the SN experiment
temperatures ranged from 17.9°C to 25.4°C, with an average of 21.3°C and relative humidity
of 61.9%. In the field experiment the average temperature was 19.8°C and relative humidity
was 73.5% during the establishment and stress period in July and August 2009. The
maximum temperature reached 36.6°C and a minimum of 7.8°C. During the stress period
there was no precipitation, providing good conditions for the drought stress treatment.
10