Appendix Table S2 Threats associated with impoundments that change ecological conditions and affect fish population distribution and abundance within relict river-fragments (1 and 2) or threaten extinction of relict populations (3) on the plains of North America, with selected examples. Threats Examples Citations 1. Habitat threats Serial discontinuity (temperature) Edwards (1978); Anderson et al. (1983) Serial discontinuity (substrate) Gore & Bryant (1986); Wilhite & Hubert (2011) Geomorphic stabilization Shields et al. (2000); Sabo et al. (2012) In-fragment land use Hoagstrom et al. (2008); O'Neill & Thorp (2011) Novel habitats Johnson (2002); Sabo et al. (2012) Water quality & quantity Hoagstrom (2009); Falke et al. (2011) Flow-regime alteration Hoagstrom et al. (2010); Perkin et al. (2011) Non-native invasions Quist et al. (2004); Falke & Gido (2006) Assemblage flux Hoagstrom et al. (2007); Falke et al. (2012) Dispersal-limitation, climate-change trap Matthews & Zimmerman (1990); Perkin et al. (2013) Diminished population/metapopulation Luttrell et al. (1999); Falke et al. (2010) Diminished effective population size Alò & Turner (2005); Osborne et al. (2005). 2. Faunal threats 3. Extinction debt Appendix 2A Sources Cited Alò D, Turner TF (2005) Effects of habitat fragmentation on effective population size in the endangered Rio Grande silvery minnow. Conservation Biology, 19, 1138-1148. Anderson KA, Beitinger TL, Zimmerman EG (1983) Forage fish assemblages in the Brazos River upstream and downstream from Possum Kingdom Reservoir, Texas. Journal Freshwater Ecology, 2, 81-88. Edwards RJ (1978) The effect of hypolimnion reservoir releases on fish distribution and species diversity. Transactions of the American Fisheries Society, 107, 71-77. Falke JA, Fausch KD (2010) , Am. Fish. Soc. Symp. 73, 207 (2010). Falke JA, Gido KB (2006) Spatial effects of reservoirs on fish assemblages in Great Plains streams in Kansas, USA. River Research and Applications, 22, 55-68. Falke JA, Bailey LL, Fausch KD, Bestgen KR (2012) Colonization and extinction in dynamic habitats: an occupancy approach for a Great Plains stream assemblage. Ecology, 93, 858-867. Falke JA, Fausch KD, Magelky R, Aldred A, Durnford DS, Riley LK, Oad R (2011) The role of groundwater pumping and drought in shaping ecological futures for stream fishes in a dryland river basin of the western Great Plains, USA. Ecohydrology, 4, 682-697. Gore JA, Bryant RM Jr. (1986) Changes in fish and benthic macroinvertebrate assemblages along the impounded Arkansas River. Journal of Freshwater Ecology, 3, 333-345. Hoagstrom CW (2009) Causes and impacts of salinization in the lower Pecos River. Great Plains Research, 19, 27-44. Hoagstrom CW, Brooks JE, Davenport SR (2008) Recent habitat association and the historical decline of Notropis simus pecosensis. River Research and Applications, 24, 789-803. Hoagstrom CW, DeWitte AC, Gosch NJC, Berry CR Jr (2007) Historical fish assemblage flux in the Cheyenne River below Angostura Dam. Journal of Freshwater Ecology, 22, 219-229. Hoagstrom CW, Zymonas ND, Davenport SR, Propst DL, Brooks JE (2010) Rapid species replacements between fishes of the North American plains: a case history from the Pecos River. Aquatic Invasions, 5, 141-153. Johnson WC (2002) Riparian vegetation diversity along regulated rivers: contribution of novel and relict habitats. Freshwater Biology, 47, 749-759. Luttrell GR, Echelle AA, Fisher WL, Eisenhour DJ (1999) Declining status of two species of the Macrhybopsis aestivalis complex (Teleostei: Cyprinidae) in the Arkansas River basin and related effects of reservoirs as barriers to dispersal. Copeia, 1999, 981-989. Matthews WJ, Zimmerman EG (1990) Potential effects of global warming on native fishes of the southern Great Plains and the Southwest. Fisheries, 15(6), 26-32. O'Neill BJ, Thorp JH (2011) Flow refugia for the zoobenthos of a sand-bed river: the role of physical-habitat complexity. Journal of the North American Benthological Society, 30, 546558. Osborne MJ, Benavides MA, Turner TF (2005) Genetic heterogeneity among pelagic egg samples and variance in reproductive success in an endangered freshwater fish, Hybognathus amarus (Cyprinidae). Environmental Biology of Fishes, 73, 463-472. Perkin JS, Bonner TH (2011) Long-term changes in flow regime and fish assemblage composition in the Guadalupe and San Marcos rivers of Texas. River Research and Applications, 27, 566-579. Perkin JS, Shattuck ZR, Gerken JE, Bonner TH (2013) Fragmentation and drought legacy correlated with distribution of burrhead chub in subtropical streams of North America. Transactions of the American Fisheries Society, 142, 1287-1298. Quist MC, Hubert WA, Rahel FJ (2004) Relations among habitat characteristics, exotic species, and turbid-river cyprinids in the Missouri River drainage of Wyoming. Transactions of the American Fisheries Society, 133, 727-742. Sabo JL, Bestgen K, Graf W, Sinha T, Wohl EE (2012) Dams in the Cadillac Desert: downstream effects in a geomorphic context. Annals of the New York Academy of Sciences, 1249, 227-246. Shields FD Jr, Simon A, Steffen LJ (2000) Reservoir effects on downstream river channel migration. Environmental Conservation, 27, 54-66. Wilhite JW, Hubert WA (2011) Longitudinal variation and habitat associations of small fishes in the Bighorn River, Wyoming: influences of river regulation and introduced fishes. Journal of Freshwater Ecology, 26, 163-170.