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Supplementary Materials
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Supplementary Table 1. Strains, plasmids and oligonucleotides used in this study.
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Relevant characteristic(s) a or sequences
Strain, plasmid,or
oligonucleotide
Source or reference
E. coli strains
WT
BW25113 (lacIqrrnBT14ΔlacZWJ16hsdR514Δara
Lab collection
WT1
BADAH33ΔrhaBADLD78)
WT carrying plasmid pUC18
This study
WT2
WT carrying plasmid pUC18-AaPal
This study
WT Δpal
pal deletion mutant of WT
This study
WT1 Δpal
pal deletion mutant of WT carrying plasmid pUC18
This study
WT2 Δpal
pal deletion mutant of WT carrying plasmid pUC18AaPal
This study
pUC18
Clone vector for use in E.coli ; Ampr
Lab collection
pUC18-AaPal
pUC18 carrying structural Aapal gene; Ampr
This study
pPZP111
binary expression vector for use in E.coli and
r
A.thaliana;
Kanr Cm
pPZP111 carrying
structural Aapal gene; Kanr Cmr
Lab collection
Plasmids
pPZP111-AaPal
Ampr
pKD46
λRed recombination system vector;
pKD4
λRed recombination system vector; Kanr
pCP20
This study
Datsenko and Wanner (2000)
Datsenko and Wanner (2000)
r
λRed recombination system vector; Amp Cm
r
Datsenko and Wanner (2000)
Oligonucleotides (primers) b
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AaPal_For
5'-GCGGAGCTCTATGAACGTACATCACGCG-3'
Amplification of AaPal, Sac I site
AaPal_Rev
5'-GCGGGATCCTTAATACACCAATACAGCG-3'
Amplification of AaPal, Bam HI site
atk1_ For
5'-AGCTTGGGTTTCGCCTTTCG-3'
Real time PCR analysis of atk1
atk1_ Rev
5'-CGATGGCGAAGAATGCGTTC-3'
Real time PCR analysis of atk1
sod_ For
5'-CCTTCTCAAGGGCCAAACCG-3'
Real time PCR analysis of sod
sod_ Rev
5'-GTGATAATCCAAGGTTTCCCGG-3'
Real time PCR analysis of sod
sos1_ For
5'-GTTGTCCCACGCAAGTTGGTC-3'
Real time PCR analysis of sos1
sos1_ Rev
5'-CTGTCGTGCAATTGTATGTGCG-3'
Real time PCR analysis of sos1
sos2_ For
5'-GGACTCAGTGCATTGCCTCAGG-3'
Real time PCR analysis of sos2
sos2_ Rev
5'-CCCTGGAAGATCCGTCTCGG-3'
Real time PCR analysis of sos2
sos3_ For
5'-AGAATGCAATGCGACCACCG-3'
Real time PCR analysis of sos3
sos3_ Rev
5'-GCGAAGAGATTCCTCCGGTTC-3'
Real time PCR analysis of sos3
hsc70_ For
5'-GCTCCAGGACTTCTTCAACGGC-3'
Real time PCR analysis of hsc70
hsc70_ Rev
5'-GGTCATGACACCACCGGCAG-3'
Real time PCR analysis of hsc70
AtActin11_ For
5’-CACATGCTATTCTGCGTTTGGAC-3'
Real time PCR analysis of AtActin11
AtActin11_ Rev
5'-TCCCTTACGATTTCACGCTCTG-3'
Real time PCR analysis of AtActin11
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Amp, ampicillin; Kan, kanamycin; Cm, chloramphenicol.
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b
Restriction endonuclease sites were underlined
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Supplementary Fig. 1 Multiple alignment of amino acids sequences of AaPal with related proteins.
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The two conserved regions in Pals are underlined and identical amino acid residues are shaded in
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black. Their GenBank accession numbers are: AaPal of Alkalomonas amylolytica (KC763932); AagPal
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(Alishewanella agri) (ZP_10350945.1); RnPal (Rheinheimera nanhaiensis) (ZP_09989187.1); PhPal
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(Pseudoalteromonas haloplanktis) (YP_340383.1); SsOmp (Shewanella sediminis) (YP_001473597.1);
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IxPal (Idiomarina xiamenensis) (ZP_11141739.1); FbPal (Ferrimonas balearica) (YP_003913563.1);
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TaPal (Tolumonas auensis) (YP_002893881.1); GpPal (Glaciecola psychrophila) (ZP_11321827.1); EcPal
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(Escherichia coli) (ZP_12055177.1); CpPal (Colwellia psychrerythraea) (YP_268470.1); AcPal,
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(Aeromonas caviae) (ZP_08518929.1).
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Supplementary Fig. 2 Cell morphology of strain WT1, WT1 Δpal and WT2 Δpal was observed by light
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microscope (upper row) and electron microscope (bottom row). E. coli cells grown in LB medium for 8
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hours were used for the analysis. Bar, 5 μm in light micrography; Bar, 1 μm in electron micrography.
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