Course Title: Advanced Biotechnology Lesson Title: Discovering the Plant Cell TEKS Addressed in Lesson: 130.364 (c) (5) http://ritter.tea.state.tx.us/rules/tac/chapter130/index.html Lesson Objectives. The student will be able to: 1 Identify the parts of a plant cell 2 Describe the functions of each organelle 3 Compare plant cells to other cells Tools and Equipment Projector PowerPoint: Identifying Plant Cells Worksheets: Plant Cell Identification and Key Key Terms / Vocabulary Cell (Plasma) Membrane Cell Wall Chloroplast Cytoskeleton Rough Endoplasmic Reticulum Plasmodesmata Golgi Complex Vacuole Mitochondria Nucleus Nucleolus Peroxisomes Ribosomes Engage / Interest Approach/Anticipatory Set Cells are the basic building blocks of life. As future professionals in biotechnology, you will need to have a strong understanding of the cell structures found in plants. These are the structures we will be working with closely to produce changes in the plant. Explore & Explain / Teaching Plan and Strategy / Presentation of New Material • Plants cells have some unique structures – In particular, chloroplasts allow the plant to capture energy from the Sun • Eukaryotic cells – Means membrane-bound nucleus • Some organelles are similar between other types of cells. • Parenchyma Cells – • Collenchyma Cells – • Synthesize and store organic products, “filler” tissue; e.g., the soft part of the plant. Extra structural support, esp. in areas of new growth Sclerenchyma Cells – Provide main structural support • Plant cells generally larger than animal • Plant cells more consistent in size and shape • Unique structures: – Cell wall – Large vacuole – Plastids • • chloroplasts Plant Cell vs Animal Cell – – Animal Cell • Various sizes and shapes • Smaller than plant cells • Organelles typically not found in plant cells: • Centrioles • Lysosomes • Cilia/flagella Plant Cell • Consistent shape and size • • Typically “cube-ish” Organelles typically not found in animal cells: • Cell wall • Large vacuole • Plastids • • Organelles: Chloroplasts – Cell (Plasma) Membrane – Cell Wall – Chloroplast – Cytoplasm – Cytoskeleton – – Golgi Complex – Microtubules – Mitochondria – Endoplasmic Reticulum (ER) – Nucleolus – Nucleopore – Peroxisomes – Plasmodesmata – Ribosomes – Vacuole Nucleus Plasma Membrane o Similar to other cell type plasma membranes o Phospholipid bilayer acts as a permeability barrier. o Location for transport of molecules into the cell. Cell Wall o Plant Cell Mostly composed of cellulose o Animal Cell No cell wall o Bacterial Cell Prokaryotic cells made up of murein (aka peptidoglycan) o The cell wall is a rigid layer on the outside of the plasma membrane. o The cell wall offers structural support, filtration, and protection. Chloroplasts o Only found in plants o Contain chlorophyll Green pigment that makes plants green o Main function is photosynthesis o Chloroplasts contain their own DNA o Chloroplasts can host transgenes – better isolated than nuclear transformation Cytoskeleton o Supports cell shape and function. o Composed of proteins which can assemble or disassemble as the needs of the cell change. o Found in cells of all domains of life. Endoplasmic Reticulum o Two types: Rough (RER) System of sacks found in cell’s cytoplasm Rough is covered with ribosomes that give it a rough appearance. Functions as transport through the cell, produces proteins Transport vesicles take proteins from RER to Golgi apparatus Smooth System of tubes located in cytoplasm. Function as transport through the cell. Contains enzymes to assist in digestion of lipids proteins Smooth ER buds off of rough ER. Golgi Complex o Also known as Golgi body or apparatus. o Flattened and layered organelle. Resembles a stack of pancakes. o Receives the vesicles from RER and converts proteins and lipids to various useable products o Transports out vesicles Microtubules o Component of the cytoskeleton o Helps maintain structure of a cell o Involved in cellular processes and intracellular transport Mitochondria o Found in most eukaryotic cells. o The “powerhouse of the cell”. o Generate most of the cell’s adenosine triphosphate (ATP) chemical energy o Maintains control over the cell cycle/growth. o Mitochondria have their own DNA (mtDNA). Nucleus o Contains most of the cell’s genetic material o Contains chromosomes which house the DNA o Nuclear pores around the nuclear membrane allow molecules to move across the membrane. o Movement is needed for gene expression and chromosome maintenance Nucleolus o Inside the nucleus is the nucleolus o Ribosomes are made inside the nucleolus Peroxisome o Produces enzymes for the breakdown of fatty acids o Assist in photorespiration of leaves and germination of seeds Plasmodesmata o Microscopic channels traversing cell walls of plant cells o Transport proteins, mRNA, viral genomes from cell to cell o Viral movement example: tobacco mosaic virus MP-30 Ribosomes o Ribosomes are technically NOT an organelle as they are non-membrane bound. o Free-floating and attached to rough endoplasmic reticulum. o Site of protein synthesis. RNA translated into protein Vacuole o Present in all plant and fungal cells Some protist, animal, and bacterial cells o Enclosed compartments filled with water o Functions: Containing waste products Containing water Maintaining internal hydrostatic pressure Support structures such as leaves and flowers Elaborate / Activity/Application/ Student Engagement /Laboratory Have students complete the computer cell identifying activity online. The URL: http://www.sheppardsoftware.com/health/anatomy/cell/plant_cell_game.htm If you wish to have them turn work in, you can opt to have them play the game and then copy the drawing and identification onto a piece of paper. Evaluation / Summary The cell identification worksheet can be utilized as an assessment or end-of-class activity. In addition, you can opt for classroom response questions such as: “What organelle is the powerhouse of the cell?” References/Additional Materials / Extended Learning Opportunities/ Enrichment Nature’s Scitable: http://www.nature.com/scitable/topicpage/plant-cells-chloroplasts-and-cellwalls-14053956 Biology about.com: http://biology.about.com/od/cellbiology/ss/plant-cell.htm Sheppard Software’s Cell Games Plant cell identification game: http://www.sheppardsoftware.com/health/anatomy/cell/plant_cell_tutorial.htm College & Career Readiness Standard Science: VI Biology, A, 1: “Know that although all cells share basic features, cells differentiate to carry out specialized functions.” ©Texas Education Agency, 2015