DEGREE IN AGRICULTURAL AND INDUSTRIAL BIOTECHNOLOGY (CLASS 1) (Joint degree course, Faculties of Agriculture and Natural Sciences) MAIN OBJECTIVES The use of biotechnology in production is becoming increasingly widespread internationally, and in the most technologically advanced countries there is currently fierce competition to be first in the experimentation phases, patenting and practical application of processes which combine efficacy and low environmental impact. In the biotechnology field, principles and methods traditionally belonging to different academic disciplines are required; as a result, professionals trained in more than one discipline and thus able to co-ordinate the various skills are highly sought after. The degree course offers two curricula: “Industrial” and “Agricultural”; the courses which distinguish the two curricula are equivalent to 40 credits. Graduates in “Agricultural and Industrial Biotechnology” must: display adequate knowledge of basic biological systems, particularly with regard to molecular and cell biology; possess knowledge of the various techniques involved in biotechnological operations for production (both cultural and experimental), using biological system forms of analysis; be familiar with each academic discipline’s methods and be able to apply them to practical situations with appropriate knowledge of current norms and the bioethical and deontological concerns; possess sufficient skills for information management and communication; be able to write technical-scientific reports; be able to work in groups, alone and adapt quickly to new work situations, thanks to work traineeships and placements in laboratories and enterprises in the sector; display adequate knowledge for resolving the many problems associated with production in the industrial and agricultural fields. The common core for both curricula provides the theoretical and applied foundation necessary for the more specific training which follows and includes: Foundation courses in statistics, information technology, physics and chemistry; Training in biological sciences, providing a foundation in biochemistry, molecular biology, genetics and microbiology; Laboratory practicals, regarding basic equipment, chemistry and cell cultures; Principles of bioethics, industrial economic law in the biotechnological sector, environmental impact of biotechnology and laboratory security. Personal study for students should be divided as follows: 68% for teaching courses and 36% for laboratory activities. The Agricultural curriculum aims to provide the graduate with the knowledge required to work in this sector, particularly regarding the study of microorganisms useful in the agro-food sector, genetic breeding techniques, fruit and vegetable crops, organic weed and pest control and molecular diagnostics. At least 30 credits are reserved for practical training, including laboratory work, excursions (such as guided tours of agro-industrial plants), short courses or placements in public or private establishments working in the sector, etc. The degree course is open to all those who satisfy the minimum university entrance requirements as prescribed by law (Italian high school diploma or foreign equivalent). Students who have successfully completed training placements carried out in collaboration with Secondary schools which have a special agreement with the University of Tuscia may have the credits earned recognised for their degree course. A summary of specific courses for the degree is given, followed by a more detailed explanation of individual course objectives and number of credits. The work traineeship can be carried out in enterprises, professional studios, companies or institutions which work in the agricultural, environmental protection or related sectors. It is equivalent to 9 credits. The student may choose courses from among those offered by the Faculty of Agriculture and by other faculties at the University of Tuscia, equivalent to 10 credits (course of student’s choice). The student may also choose courses offered by other Italian or foreign universities, once prior authorisation has been given by the Degree Course Council. The student may also substitute (for equal credits) two core or complementary subjects of the current degree course with other courses offered by the Faculty of Agriculture, once prior authorisation has been sought from the Degree Course Council. The examinations include written tests during the courses, discussion of any projects, assignments and seminars and final written and/or oral examinations. The final examination involves an original dissertation, either compiling information or representing research aimed at demonstrating the student’s ability to plan and carry out an experimental process in the agricultural or industrial sector. Three-year course timetable for Agricultural and Industrial Biotechnology degree, divided into terms. Ist Year: Core subjects for both Industrial and Agricultural curricula Term Subject Credits Lecturer (2001-2002) I Mathematics and statistics 6 Scarelli biology 5+1 Cottarelli English 4 II Physics 5+1 Information technology 3+1 Bioethics 5 International law on biotechnology 5 III General chemistry 6+1 Cecchi Bio-computing 2 Caporale Principles of industrial economics 5 Animal cell cultures 1+2 Willems Plant cell cultures 1+2 Mazzucato Work traineeship/ placement etc. 4 IV V VI VII 2nd Year: Agricultural curriculum Organic chemistry 6+1 Genetics 4+2 Agricultural chemistry 5+21 Biological chemistry 5+1 Microbiology 5+1 Plant biology 3+2 Molecular biology and genetic manipulation techniques 5+1 Plant physiology and biotechnology 5+1 Course of student’s choice 5 Work traineeship/placement, etc. 5 3rd Year: Agricultural curriculum Advanced genetic breeding 5+2 In vitro cultures and cell manipulation in organisms of agricultural interest 5 Environmental impact and bio-security 5 VIII Microorganism biotechnology I 3+2 Microorganism biotechnology II 3+2 Fruit crop biotechnology 4+1 Phytopathological biotechnology 4+1 IX Course of student’s choice 5 Foreign language laboratory 3+2 Final examination 11 Work traineeship/placement, etc. 2 Saladino Palitti Giovannozzi Buonocore Federici Tucci Masci, Ceoloni D’Ovidio De Pace, Soressi Rugini Ruzzi Canganella Muleo Vannini Lafiandra, Description of individual course objectives and number of credits 2ND YEAR 2ND YEAR: 4TH TERM ORGANIC CHEMISTRY (7 CREDITS) GENETICS (6 CREDITS) AGRICULTURAL CHEMISTRY (7 CREDITS) Provide the basis for understanding the biochemical mechanisms which are fundamental in plant tissues. 5TH TERM BIOLOGICAL CHEMISTRY (6 CREDITS) MICROBIOLOGY (6 CREDITS) PLANT BIOLOGY (5 CREDITS) Study the distinguishing characteristics of plant cells; knowledge of morphology, anatomy and functions of plant organs as regards development; knowledge of formation and constitution of main types of wood; fundamental principles in plant reproduction and propagation; knowledge of phylogenetic principles and the natural classification system; knowledge of artificial classification systems; theoretical and practical knowledge of main taxa relevant for forest environments. 6TH TERM MOLECULAR BIOLOGY AND GENETIC MANIPULATION TECHNIQUES (7 CREDITS) Provide the foundations for understanding the organisation and structure of the plant genome and the basis of genetic and molecular biotechnology which permit genome manipulation for improving traits of both agricultural plants and potentially interesting wild plants. PLANT PHYSIOLOGY AND BIOTECHNOLOGY (6 CREDITS) Provide the foundation for understanding plant physiological processes and the basis for applying possible biotechnological strategies, with the aim of improving certain specific agro-food characteristics and the safeguard of the environment. 7TH TERM ADVANCED GENETIC BREEDING (7 CREDITS) The aim of the course is to present and discuss, with laboratory practicals where appropriate, the new strategies in genetic breeding to assess and use the genetic resources of plant species to constitute new cultivars. Classical methods (hybridisation and selection) and innovative methods (genome analysis, genetic transformation and in vitro culture) are considered. More specifically, combinations of methods will be demonstrated which allow the use of genetic resources both in order to safeguard the environment and biodiversity (quantity and quality factors) of food of plant origin. IN VITRO CULTURES AND CELL MANIPULATION AGRICULTURAL INTEREST (6 CREDITS) IN ORGANISMS OF Provide the foundation for applying in vitro propagation techniques, techniques for regeneration of organs from differentiated and undifferentiated tissues, for the induction of somaclonal variability and for transferring genes in agricultural plants. ENVIRONMENTAL IMPACT AND BIO-SECURITY (5 CREDITS) 8TH TERM MICROORGANISM BIOTECHNOLOGY I & II (10 CREDITS) Provide foundations for understanding the potential applications of microorganisms when isolated from either land or water environments. Extreme environments and isolation of microorganisms for biodegradative applications and/or for hydrolytic enzyme production. The utilisation of DNA recombinant techniques for studying microorganisms of agro-food interest and the development of genetically manipulated microorganisms for use in: synthesis of commercial products and proteins; bio-correction and use of biomass; promotion of plant growth; bio-control. Legislation and risks linked to release of genetically manipulated microorganisms in the environment. FRUIT CROP BIOTECHNOLOGY (5 CREDITS) Provide the foundation for understanding physiological and molecular processes of growth, development and production of fruit trees. Indication of possible strategies in biotechnology use in order to improve specific traits with strong agronomic and implications. Use of molecular markers for assisted selection and for genetic breeding. PHYTOPATHOLOGICAL BIOTECHNOLOGIES (5 CREDITS)