UCSF Lab Standard Operating Procedure
Chemical Process, Name or Hazard Class:
Ozone
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Type of SOP:
☐ Process
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☒Hazardous Chemical
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Purpose
Ozone is triatomic oxygen. It may be fatal if inhaled or absorbed through the skin and may be harmful if
ingested. It causes irritation to the gastrointestinal tract, respiratory tract, skin, and eyes. Ozone is a strong
oxidizing agent and a natural part of the atmosphere. It can be produced from the combustion of fossil fuels. It
has several industrial and commercial applications. It is used as a disinfectant and deodorizer for air and
water and as a bleaching agent for food, waxes, textiles, and paper. It is also used in waste treatment and in
organic synthesis.
Physical & Chemical Properties/Definition of Chemical Group
CAS#: 10028-15-6
Class: Acute Toxicant, oxidizer
Molecular Formula: O3
Form (physical state): Gas
Color: Colorless, light blue
Boiling point: -112 °C
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Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)
Potential Hazards/Toxicity
Ozone may be fatal if inhaled or absorbed through the skin and may be harmful if ingested. It causes irritation
to the gastrointestinal tract, respiratory tract, skin, and eyes. Symptoms of exposure include headache,
coughing, dry throat, shortness of breath, chest tightness, upset stomach, vomiting, pulmonary edema,
asthma, and lung damage. It is highly unstable and highly reactive. It is a propellant. Ozone has a permissible
exposure limit (PEL) of 0.1 ppm, and is immediately dangerous to life and health (IDLH) at 5ppm. It has a
pungent odor at 0.01ppm, well below the IDLH.
Engineering Controls
Work with this chemical in a certified ducted fume hood. Facilities storing or utilizing this material should be
equipped with an eyewash facility and a safety shower.
Personal Protective Equipment (PPE)
Respirator Protection
If lab personnel would like to use respirator on a voluntary basis, they must be trained and fit-tested by
EH&S. This is a regulatory requirement. (http://www.ehs.ucsf.edu/respiratory-protection-program)
Hand Protection
Handle with nitrile or chloroprene gloves. Gloves must be inspected prior to use. Use proper glove removal
technique (without touching glove's outer surface) to avoid skin contact with this product. Dispose of
contaminated gloves after use in accordance with applicable laws and good laboratory practices. Wash and
dry hands.
NOTE: Consult with your preferred glove manufacturer to ensure that the gloves you plan on using are
compatible with ozone.
Refer to glove selection chart from the links below:
http://www.ansellpro.com/download/Ansell_8thEditionChemicalResistanceGuide.pdf
OR
http://www.allsafetyproducts.biz/page/74172
OR
http://www.showabestglove.com/site/default.aspx
OR
http://www.mapaglove.com/
Eye Protection
ANSI approved safety glasses or goggles.
Skin and Body Protection
Lab coats should be worn. These laboratory coats must be appropriately sized for the individual and be
buttoned to their full length. Laboratory coat sleeves must be of a sufficient length to prevent skin exposure
while wearing gloves. Full length pants and close-toed shoes must be worn at all times by all individuals that
are occupying the laboratory area. The area of skin between the shoe and ankle should not be exposed.
Hygiene Measures
Avoid contact with skin, eyes and clothing. Wash hands before breaks and immediately after handling the
product.
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Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)
First Aid Procedures
If inhaled
Move person into fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen.
Take victim immediately to hospital. Consult a physician.
In case of skin contact
Wash off with soap and plenty of water for at least 15 minutes while removing contaminated clothing and
shoes. Consult a physician.
In case of eye contact
Flush with plenty of water for at least 15 minutes lifting upper and lower eyelids and removing contact
lenses. Consult a physician.
If swallowed
Do not induce vomiting. Never give anything by mouth to an unconscious person. Rinse mouth with water.
Consult a physician.
Special Handling and Storage Requirements
Precautions for safe handling: Avoid contact with skin and eyes. Avoid inhalation and ingestion. Ensure
normal measures for preventive fire protection.
Conditions for safe storage: Store in secondary containment with acute toxin label on the primary
container, secondary containment and the storage location. Keep containers tightly closed in a dry, cool, and
well-ventilated place. It readily reacts and decomposes to O2 under normal ambient temperatures.
Incompatible with oxidizable materials both organic and inorganic, rubber, dicyanogen, dinitrogen tetroxide,
4-hydroxy-4-methyl-1,6-heptadiene, nitrogen trichloride, stibine, and tetrafluorohydrazine. Explosive with
alkenes, aromatic compounds, bromine, combustible gases, diethyl ether, hydrogen bromide, and
isopropylidene compounds. Ignites upon contact with alcohols, amines, ammonia, beryllium alkyls, boranes,
dicyanogen, hydrazines, hydrocarbons, hydrogen, nitroalkanes, powdered metals, silanes, or thiols.
Spill and Accident Procedure
Chemical Spill Dial 9-911 from campus phone or 415-476-1414 from cell phone or 415-2068522 (SFGH only)
Spill – Assess the extent of danger. Assist contaminated or injured persons. Evacuate the spill area.
Avoid breathing vapors. If possible, confine the spill to a small area using a spill kit or absorbent
material. Keep others from entering contaminated area (e.g., use caution tape, barriers, etc.).
Small (<1 L) – If you have training, you may assist in the clean-up effort. Use appropriate personal
protective equipment and clean-up material for chemical spilled. Double bag spill waste in clear
plastic bags, label and take to the next chemical waste pick-up.
Large (>1 L) – Dial 9-911 from campus phone or 415-476-1414 from cell phone or 415-2068522 (SFGH only) for assistance.
Chemical Spill on Body or Clothes – Remove clothing and rinse body thoroughly in emergency
shower for at least 15 minutes. If discomfort persists, proceed to the Emergency Department. If no
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Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)
further discomfort is experienced, have the SDS ready and contact Poison Control Hotline at 1-800222-1222 for further exposure information. Notify your direct supervisor and EH&S at 415-4761300 during work hours, or 9-911 during non-working hours and weekends.
Chemical Splash Into Eyes – Immediately rinse eyeball and inner surface of eyelid with water for
15 minutes by forcibly holding the eye open. If discomfort persists, proceed to the Emergency
Department. If no further discomfort is experienced, have the SDS ready and contact Poison
Control Hotline at 1-800-222-1222 for further exposure information. Notify your direct
supervisor and EH&S at 415-476-1300 during work hours, or 9-911 during non-working
hours and weekends.
Medical Emergency Dial 9-911 (campus phone) or 476-6911 (cell phone)
Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8 hours.
Non-Life Threatening Emergency– Go to Occupational Health Programs (OHP) Clinic, 415-8857580, 2330 Post Street, Suite 460 Hours of Operation for Appointments: Monday - Friday
7:30 a.m. - 4:00 p.m. (except Holidays).
Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8 hours.
Needle stick/puncture exposure (as applicable to chemical handling procedure) – Wash the
affected area with antiseptic soap and warm water for 15 minutes. For mucous membrane
exposure, flush the affected area for 15 minutes using an eyewash station. Page the needle stick
nurse by dialing 415-353-7842 (STIC).
Decontamination/Waste Disposal Procedure
Clean contaminated surfaces with soap and water and paper towels. Dispose of the paper towels as
hazardous waste.
Safety Data Sheet (SDS) Location
Online SDS can be accessed at http://www.ehs.ucsf.edu/safety-data-sheet-sds-1
Protocol/Procedure
Quantities covered by this SOP:
______ (g , ml) to _______ (g, ml)
Temperature range covered by this SOP:
__ °C – __ °C
General Overview and Purpose:
Enter the experimental purpose
Procedure:
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Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)
Enter experimental procedure. You can copy procedure from your lab notebook or from literature.
NOTE
Any deviation from this SOP requires approval from the Principal Investigator.
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Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)