wrong 0 1 2 3 4 5 6 7 8 9 10 11 12 right 59 58 57 56 55 54 53 52 51 50 49 48 47 score 100.0 98.3 96.6 94.9 93.2 91.5 89.8 88.1 86.4 84.7 83.1 81.4 79.7 Soil Analysis Group Name Name _____________________________________ Today you will test some soil for five characteristics: soil texture, acidity, nitrogen content, phosphorus content, and potassium content. These characteristics determine how well plants will grow in a particular soil. Soil Texture Soil Source _____________________ The texture of a soil determines the amount of air and water the soil can hold. Plant roots need liberal supplies of both. Air is critical as a source of oxygen gas and as a repository for excess carbon dioxide gas. These gases are needed for and produced by respiration in root cells, respectively. If there is not much air space in the soil, then roots "suffocate" because they cannot carry out respiration. The roots then die, the plant wilts, and then dies. Large soil particles do not pack tightly and therefore provide air spaces in the soil. On the other hand, soil consisting of extremely fine particles packs tightly and permits little air in the soil to support root function. Water from the roots is the supply of this important chemical for the shoot system (stem, leaves, flowers, fruits, and seeds). The water is a reactant in photosynthesis and a product of respiration. Water makes up about 90% of plant cells by weight, and is the critical solvent in the cell. The evaporation of water through the stomata in the epidermis of the shoots cools the plants and prevents overheating. A soil with very large particles drains too extensively and plants will lack sufficient water, will wilt, and perhaps even die. A soil with extremely fine particles holds tremendous amounts of water and can hold so much as to exclude air from the soil. In that case, the roots die, the plants wilt, and perhaps will die as well. Thus, the perfect soil texture for growing plants is a compromise between fine particles (clay), medium particles (silt), and coarse particles (sand). The range of soil textures that support plant growth are called "loam." A. AT LEAST FIVE HOURS BEFORE LAB! Fill a 100 mL graduate cylinder to the 70 mL mark with sifted soil. Fill the cylinder with sodium hexametaphosphate (surfactant) solution (2 teaspoons of Calgon per quart). Cover the top of the cylinder with plastic wrap. Stir/agitate the soil completely and thoroughly so that there is no unmixed soil at the bottom of the cylinder. Top-off the cylinder with surfactant. Continue inversion-stirring for at least five minutes. Label the cylinder with your group name. Set the cylinder aside until the next class meeting in a place where it will not be disturbed. B. On the regular Lab Exercise day, and disturbing the cylinder as little as possible, measure the volume of the sand layer in the bottom of the cylinder. Since this is the coarsest particle size, it will have dark voids between particles (ask your instructor to help you distinguish the sand from the silt). Volume of the sand layer ________ mL C. Determine the volume of the sand + silt layers. The measuring line will appear just below the clay layer which is very smooth and usually light in color. Silt has a few voids and the particles are visible; clay has no voids and the particles are too small to be visible. Volume of the sand + silt layers ________ mL D. Determine the volume of the sand + silt + clay layers. The measuring line will appear between the clay layer and the dark water above it. Volume of the sand + silt + clay layers ________ mL /4 Page 2 E. Calculate the volume of the silt layer by subtracting the number from part B above from the number in part C above. Volume of silt layer ________ mL F. Calculate the volume of the clay layer by subtracting the number from part C above from the number in part D above. Volume of clay layer ________ mL G. Rinse out your cylinder completely and thoroughly as described by the instructor. Dry it with a paper towel, and return it to the proper location. H. Calculate the percentage contribution for each of the three layers by dividing their individual volumes by the total soil volume (D). If your calculations are correct, the sum of these percentages should be 100! Check your work! Sand ______% Silt ______% Clay ______% Total ______% G. Place a small X on the diagram below where the three layer percentages would plot out together. What is the texture classification for this soil? 100 0 90 10 80 70 ___________________________ 20 30 clay 60 Percent Clay 50 40 sandy clay silt y clay 40 30 sandy clay clay 0 100 silt y loam loam clay 60 loam 70 loam 20 10 Percent Silt 50 80 silt loamy sandy loam sand sand loam 90 silt 100 90 80 70 60 50 40 30 20 10 0 Percent Sand /10 Page 3 Soil Acidity (pH) The amount of acid or alkalai in the soil determines the availability of many nutrients for plant growth and maintenance. It is important that the soil have the correct balance of acid and alkalai for the amount of nutrients to be released from the soil particles for their use. The balance is measured on a scale of acidity called pH. The scale goes from 1 (very acid) to 14 (very alkaline) with the neutral pH of 7 in the middle of the scale. Distilled water has a pH of 7, vinegar is typically pH 4, and soap has a pH of about 10. If the pH of soil is too high or too low, the nutrients are either locked onto the soil particles or are washed out of the soil by rain. Even applications of fertilizer to such a soil are useless and wasted. Most plants grow best when the soil pH is between 5.5 and 6.5 (on the slightly acid side of neutral). If soil tests too low, the pH can be raised by applying lime (calcium and magnesium compounds). If soil tests too high, the pH can be lowered by applying sulfur or aluminum sulfate. You will use a commercial soil test kit to determine the pH of your soil sample. The instructor will dissolve two capsules of pH test powder into 10 mL of distilled water to make the pH test reagent for the class. A. Put your group symbol on the cap of the microfuge tube. B. Using a drinking straw, place 0.25 mL of sifted soil into the microfuge tube. C. Add 1 mL of the pH test reagent. D. Close the cap and mix the contents of the tube by shaking it for 1 full minute. E. Centrifuge the tube for 30 seconds at 14,000g. F. Compare the color of the transparent solution above the soil layer with the pH color chart Circle the pH indicated next to the color swatch matching most closely the color of the solution: 7.5 7.0 6.5 6.0 5.5 5.0 4.5 G. Calculate the type and amount of soil additive needed to adjust a 50 ft. x 100 ft. garden of the soil you sampled to pH 6.5. Assume it takes 68 lbs. of ground limestone per 1000 sq. ft. to raise the pH 1 unit. Assume it takes 15 lbs. of aluminum sulfate per 1000 sq. ft. to lower the pH 1 unit. If your soil test indicates the pH is already 6.5, then do the calculation below assuming your results were pH 5.5 instead. Name of Additive needed _________________________________ pH change needed ______ units Amount of additive to use on 1000 sq. ft. to change to pH 6.5 _______ lbs. Area (=width x length) of 50' x 100' garden __________ sq. ft. Amount of additive to use on 50' x 100' garden _______ lbs. /6 Page 4 Nitrogen Content An appropriate supply of nitrogen gives plants healthy dark-green foliage. It promotes the growth of vegetative parts of the plant (root, stem, leaf). It should be abundant for crops like grass, cabbage, asparagus, onions, lettuce, and spinach. Too much nitrogen, however, can cause growth to be too rapid, can cause the plant to grow tall and soft, to fall over, and can reduce yield. Excess nitrogen can also delay and prevent flower and fruit formation in other crops. Therefore, you must compromise between the high nitrogen levels needed for true vegetables and the lower amounts needed for fruit crops (beans, corn, squash, peas, strawberries, etc). Plants lacking sufficient nitrogen will be short, thin, and yellowish green, particularly in the lower leaves. The leaves will turn yellow, and will brown down to a crispy state. In monocots, such as grasses, the tip of the leaf browns first and the browning progresses toward the leaf sheath. The instructor will dissolve two capsules of Nitrogen test powder into 5 mL of distilled water to make the Nitrogen test reagent for the class. A. Put your group symbol on the cap of a microfuge tube. B. Using a drinking straw, place 0.5 mL of sifted soil into the microfuge tube. C. Add distilled water from the red wash bottle to reach the 1.5 mL mark. D. Close the cap and mix the contents of the tube by shaking it for 1 full minute. E. Centrifuge the tube for 30 seconds at 14,000g. F. Disturbing the liquid as little as possible, transfer 0.5 mL of the clear liquid above the soil layer into a glass 13x100 mm test tube. G. The instructor will add 0.5 mL of Nitrogen test reagent to your glass tube. H. Cover the glass tube with a bit of plastic film and shake it for 1 minute. I. Let the tube stand in the test-tube rack for 10 minutes for full color development. J. Compare the magenta color of the solution to the Nitrogen color chart. Locate the color swatch matching most closely the color of the solution. Circle the corresponding amount of Nitrogen needed for your soil (lbs/1000 sq ft) in the chart below: N4 Surplus N3 Sufficient N2 Adequate N1 Deficient N0 Depleted 0.07 0.15 0.3 1.4 2.7 K. Calculate the amount of fertilizer (Ammonium nitrate = 33 percent nitrogen content) needed for your 50' x 100' garden. Divide the amount circled above by the percentage of N in fertilizer (0.33) = ________ lbs Ammonium nitrate/1000 sq ft Amount of Ammonium nitrate to buy for your whole garden: ________ lbs /3 Page 5 Phosphorus Content Phosphorus is essential for flower, fruit, and seed production. It is an important part of DNA (the genetic molecule). It also facilitates seed germination. The supply of phosphorus determines, in part, the rate that the plants reach sexual maturity. It is usually difficult to have too much phosphorus because the supplies in soil are typically so limited. Plants lacking sufficient phosphorus usually have purplish leaves, petioles, and stems. They grow slowly and mature very late in the season (if then). The yield of crops like corn, beans, peas, squash, cucumber, etc. will be very low under phosphorus deficiency. The instructor will dissolve two capsules of Phosphorus test powder into 5 mL of distilled water to make the Phosphorus test reagent for the class. A. Put your group symbol on the cap of a microfuge tube. B. Using a drinking straw, place 0.5 mL of sifted soil into the microfuge tube. C. Add distilled water from the red wash bottle to reach the 1.5 mL mark. D. Close the cap and mix the contents of the tube by shaking it for 1 full minute. E. Centrifuge the tube for 30 seconds at 14,000g. F. Disturbing the liquid as little as possible, transfer 0.5 mL of the clear liquid above the soil layer into a glass 13x100 mm test tube. G. The instructor will add 0.5 mL of Phosphorus test reagent to your glass tube. H. Cover the glass tube with a bit of plastic film and shake it for 1 minute. I. Let the tube stand in the test-tube rack for 10 minutes for full color development. J. Compare the blue color of the solution to the Phosphorus color chart. Locate the color swatch matching most closely the color of the solution. Circle the corresponding amount of Phosphorus needed for your soil (lbs/1000 sq ft) in the chart below: P4 Surplus P3 Sufficient P2 Adequate P1 Deficient P0 Depleted 0.2 0.4 0.8 1.6 3.2 K. Calculate the amount of fertilizer (Superphosphate = 18 percent phosphorus content) needed for your 50' x 100' garden. Divide the amount circled above by the percentage of P in fertilizer (0.18) = ________ lbs Superphosphate/1000 sq ft Amount of Superphosphate to buy for your whole garden: ________ lbs /3 Page 6 Potassium Content Potassium is important for carbohydrate (sugar and starch) manufacture by plants. When sufficient potassium is available, plants produce stiff, erect stems, and the plants are more disease resistant. When insufficient or excess potassium is in the soil, plants contain too much water, are susceptible to frost injury, and growth is reduced. Since roots are important storage areas for carbohydrate, root crops like carrot, turnip, and radish and tuber crops like potatoes are enhanced by supplemented potassium. Plants with deficient potassium have mottled, spotted, or streaked leaves. The leaves curl strongly at the ends. The leaf margins die and flake out, leaving a ragged edge. Poor root development may lead to the plant toppling over as the stem grows. The instructor will dissolve two capsules of Potassium test powder into 5 mL of distilled water to make the Potassium test reagent for the class. A. Put your group symbol on the cap of a microfuge tube. B. Using a drinking straw, place 0.5 mL of sifted soil into the microfuge tube. C. Add distilled water from the red wash bottle to reach the 1.5 mL mark. D. Close the cap and mix the contents of the tube by shaking it for 1 full minute. E. Centrifuge the tube for 30 seconds at 14,000g. F. Disturbing the liquid as little as possible, transfer 0.5 mL of the clear liquid above the soil layer into a glass 13x100 mm test tube. G. The instructor will add 0.5 mL of Potassium test reagent to your glass tube. H. Cover the glass tube with a bit of plastic film and shake it for 1 minute. I. Let the tube stand in the test-tube rack for 10 minutes for full color development. J. Compare the amber color of the solution to the Potassium color chart. Locate the color swatch matching most closely the color of the solution. Circle the corresponding amount of Potassium needed for your soil (lbs/1000 sq ft) in the chart below: K4 Surplus K3 Sufficient K2 Adequate K1 Deficient K0 Depleted 0.2 0.4 0.9 1.7 3.4 K. Calculate the amount of fertilizer (Potassium Sulfate = 50 percent potassium content) needed for your 50' x 100' garden. Divide the amount circled above by the percentage of K in fertilizer (0.50) = ________ lbs Potassium Sulfate/1000 sq ft Amount of Potassium Sulfate to buy for your whole garden: ________ lbs /3 Page 7 Using Other Single Fertilizers The calculations you have performed for your 50' x 100' garden were based upon use of a single fertilizer that contained an abundance of a single nutrient (N, P, or K). These calculations allow you to optimize the amount of each of the nutrients for your particular soil. It also turns out that this is probably the most inexpensive way to fertilize, providing you have learned how to do the calculations. Most people do not have the knowledge to do the calculations, and many do not live near agricultural supply companies (there is just one in the Willimantic area). How would the calculations be different if you had to use a different single fertilizer? Amount circled on Amount needed per Amount needed for Single Fertilizer a previous page 1000 sq ft 50x100 ft garden Nitrate of Soda 16%N Triple Superphosphate 46%P Muriate of Potash 60%K Page 4: Page 5: Page 6: Using “Balanced” Fertilizers “Balanced” fertilizers contain a mixture of all three major nutrients (N, P, and K). The amount of each nutrient in the mixture is shown on the packaging as the guaranteed analysis. By law, the sequence of numbers in the analysis is always the percentage of N-P-K. So a fertilizer with an analysis of 5-10-15 contains 5% N, 10% P, and 15% K. Various combinations of nutrients are available. Repeat your calculations but this time imagine you are a sheep farmer: Amount circled on a Amount needed per Amount needed for Sheep Manure previous page 1000 sq ft 50x100 ft garden Page 4: 0.8%N Page 5: 0.3%P Page 6: 0.9%K Obviously it is quite unlikely that a single amount of sheep manure will perfectly balance your soil nutrients, but a person might choose to apply the greatest amount shown above, hoping it is not too much of an overdose of the other nutrients. Using Commercial “Balanced” Fertilizers At a garden store, you can purchase a box of fertilizer that has the guaranteed analysis of 6-12-10. Repeat your calculations using this commercial fertilizer: Amount circled on a Amount needed per Amount needed for 6-12-10 Fertilizer previous page 1000 sq ft 50x100 ft garden %N %P %K Page 4: Page 5: Page 6: /30 Page 8 Here is a listing of other fertilizers that might be less-expensive than boxed blends: Material Animal Tankage Bloodmeal Bone Meal Castor Pomace Cocoa Shells Coffee Grounds (dried) Cottonseed Meal Cow Manure Legume Waste Fish Scrap Fly Ash Granite Dust Greensand Mineral Phosphate Guano Chicken Manure Horse Manure Hoofmeal and Horndust %N 8 15 4 5.5 2.7 2.0 8.0 0.6 3.0 8 0 0 0 0 12 1.1 0.7 12.5 %P 20 1 25 1.5 1.5 0.3 2.5 0.2 0.5 13 0 0 1.5 29 8 0.8 0.3 1.5 %K 0 0.5 0 1 2.7 0.5 1.5 0.5 2 4 12 5 7 0 3 0.5 0.6 0 Material Incinerator Ash Milorganite Sodium Nitrate Peanut Shells Phosphate Rock Pig Manure Seaweed Sheep Manure Slag Sludge Steer Manure Sugar Wastes Ammonium Sulfate Super Phosphate Tobacco Stem Powder Triple Super Phosphate Urea Wood Ashes %N 0.2 6 16 3.6 0 0.5 1.5 0.8 0 6 0.8 0 21 0 3.3 0 45 0 %P 5 3 0 0 30 0.3 1 0.3 8 3 0.3 8 0 18 0 47 0 1.5 %K 2 0 0 0 0 0.4 5 0.9 0 0 0.4 0 0 0 7.0 0 0 8 Here is a listing of plants (by category) to tell of their fertilizing needs: L=low M=medium H=high VH=very high Flowers, perennials, bulbs & herbs Amaryllis Bleeding Heart Lily of valley Parsley Coreopsis Gardenia Daffodil Ageratum Candytuft Snapdragon Tulip Alyssum Begonia Bell Flower Calendula Canna Carnation Clematis Coleus Columbine Cone Flower Crocus Dahlia Day Lily Foxglove Gaillardia Geranium Gladiolus Gypsophila Hibiscus Hollyhock Hydrangea Larkspur Loosestrife Marjoram Mint Nasturtium Pansy Peony Perwinkle Petunia Phlox Poinsettia Poppy Primrose Verbena Violet Yucca Chrysanthemum Iris Zinnia N M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M P M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M K M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M pH 5.0-6.0 5.0-6.0 5.0-6.0 5.0-7.0 5.5-6.5 5.5-6.5 6.0-6.5 6.0-7.0 6.0-7.0 6.0-7.0 6.0-7.0 6.0-8.0 6.0-8.0 6.0-8.0 6.0-8.0 6.0-8.0 6.0-8.0 6.0-8.0 6.0-8.0 6.0-8.0 6.0-8.0 6.0-8.0 6.0-8.0 6.0-8.0 6.0-8.0 6.0-8.0 6.0-8.0 6.0-8.0 6.0-8.0 6.0-8.0 6.0-8.0 6.0-8.0 6.0-8.0 6.0-8.0 6.0-8.0 6.0-8.0 6.0-8.0 6.0-8.0 6.0-8.0 6.0-8.0 6.0-8.0 6.0-8.0 6.0-8.0 6.0-8.0 6.0-8.0 6.0-8.0 6.0-8.0 6.0-8.0 6.8-8.0 6.8-8.0 6.8-8.0 Shrubs, ornamentals, etc. Camellia Azalea Rhododendron Junipers Yew Grass Barberry Bayberry Boxwood Butterfly Bush Cotoneaster Euonymus Forsythia Lilac Privet Rose Spirea Wisteria N L L L L L L L L L L L L L L L L L L P L L L L L L L L L L L L L L L L L L K L L L L L L L L L L L L L L L L L L pH 4.0-5 5 5.0-6.0 5.0-6.0 5.5-7.0 5.5-7.0 6.0-7.0 6.0-8.0 6.0-8.0 6.0-8.0 6.0-8.0 6.0-8.0 6.0-8.0 6.0-8.0 6.0-8.0 6.0-8.0 6.0-8.0 6.0-8.0 6.0-8.0 Trees Mountain Ash Hemlock Holly Magnolia Pine Spruce Oak, White Beech Dogwood Oak, Red Spruce, Colorado Arborvitae Maple Redbud N M L L M L L M M M M L L M M P L L L L L L L L L L L L L L K L L L L L L L L L L L L L L pH 4.0-5.0 5.0-6.0 5.0-6.0 5.0-6.0 5.0-6.0 5.0-6.0 5.5-7.0 6.0-7.0 6.0-7.0 6.0-7.0 6.0-7.0 6.0-8.0 6.0-8.0 6.0-8.0 Fruits & vegetables Potato Strawberries Potato, Sweet Apples Bean Carrot Eggplant Corn, Sweet Beet Watermelon Broccoli Cabbage Pumpkin Tomato Brussels Sprouts Cauliflower Lettuce Onion Pea Radish Asparagus Blackberries Canteloupe Cucumber Grape Squash Spinach N M M L M L M H H H M H H H M H H H H M M VH L M H M H VH P H M M L M H H H VH M H H H VH H H VH H H VH H L M H M VH VH K H M H L M H H H H M H H H VH H VH VH H H VH VH L M H M VH VH pH 4.5-6.5 5.0-6.0 5.0-7.0 5.5-6.5 5.5-6.5 5.5-6.5 5.5-6.5 5.5-7.5 5.8-7.0 6.0-7.0 6.0-7.0 6.0-7.0 6.0-7.0 6.0-7.0 6.0-7.5 6.0-7.5 6.0-7.5 6.0-7.5 6.0-7.5 6.0-7.5 6.0-8.0 6.0-8.0 6.0-8.0 6.0-8.0 6.0-8.0 6.0-8.0 6.4-7.0 If you had no soil test results to help you determine which fertilizer to use, a good "shot in the dark" for general purpose would be 20-20-20 (a balanced fertilizer). For your vegetable garden 510-5 would be generally useful, but for fruit crops 5-15-5 would be better. For your lawn 20-5-5 or 20-0-0 would be appropriate. Understand however, that fertilization without a soil analysis can waste time, effort, and money, and is likely to not produce ideal conditions. Your local agricultural extension service can perform soil analyses for a modest fee. For this area UCONN is the appropriate contact.