Hellenic Accreditation System S.A.
Annex F1/3 to the Certificate No. 05-2
SCOPE of ACCREDITATION
of the
Institute of Food Hygiene of Athens
of the
Centre of Athens Veterinary Institutions
of the
Ministry of Rural Development and Food
Materials/ Products tested
Types of test/ Properties measured
Applied Methods/Techniques used
Chemical tests
1. Meat, liver and kidney
Determination of cadmium (Cd) content
In-house method using Atomic
Absorbtion Spectrometry with
Graphite Furnace (ZET-AAS)
2. Fish food and fishery
products
Determination of cadmium (Cd) content
In-house method using Atomic
Absorbtion Spectrometry with
Graphite Furnace (ZET-AAS)
3. Fish food and
products
Determination of mercury (Hg) content
In-house method using Atomic
Absorption Spectrometry with Flow
Injection – Mercury Hydride (FI-MHAAS)
Page 1 of 4
Annex F1/3 to the Certificate ESYD No 05 -2
22.7.2009
Materials/ Products tested
Types of test/ Properties measured
Applied Methods/Techniques used
1. Determination of malachite green (and its
metabolite leucomalachite)
In house method based on the
corresponding method of the
European Community Reference
Laboratory AFSSA using High
Performance Liquid Chromatography
with Diode Array Detector (HPLCDAD), validated according to the
requirements of the Decision
EU/657/2002.
2. Determination of malachite green,
leucomalachite and crystal violet
In house LC-MS/MS method,
validated according to the
requirements of the Decision
EU/657/2002.
5. Animal tissues
Determination of tetracycline residues (4):
Tetracycline, Oxytetracycline,
Chlortetracycline, Doxycycline, 4Εpichlortetracycline
In house method based on the
corresponding method of the
European Community Reference
Laboratory AFSSA using High
Performance Liquid Chromatography
-Mass Spectrometry (LC-MS) (ESIpositive mode), validated according to
the requirements of the Decision
EU/657/2002.
6. Muscular tissues
Determination of quinolone residues (7):
Ciprofloxacin, Danofloxacin, Difloxacin,
Enrofloxacin, Flumequine, Marbofloxacin,
Oxonilic acid
In house method based on the
corresponding method of the
European Community Reference
Laboratory AFSSA using High
Performance Liquid Chromatography
with FLD (HPLC-FLD), validated
according to the requirements of the
Decision EU/657/2002.
7. Animal tissues
Determination of sulfonamide residues (10):
Sulfadiazine, sulfathiazole, sulfamerazine,
sulfadimidine, sulfamethoxypyridazine,
sulfamonomethoxine, sulfachlorpyridazine,
sulfadoxine, sulfadimethoxine,
Na-sulfaclozine
In house method based on the
corresponding method of the
European Community Reference
Laboratory AFFSA using High
Performance Liquid Chromatography
with DAD (HPLC-DAD), validated
according to the requirements of the
Decision EU/657/2002.
8. Milk
Determination of sulfonamide residues (7):
Sulfadiazine, sulfamerazine, sulfadimidine,
sulfamethoxypyridazine,
sulfachlorpyridazine, sulfadoxine,
sulfadimethoxine
In house method based on the
corresponding method of the
European Community Reference
Laboratory AFFSA using High
Performance Liquid Chromatography
with Fluorescence Detector (HPLCFLD), validated according to the
requirements of the Decision
EU/657/2002.
4. Fish
Page 2 of 4
Annex F1/3 to the Certificate ESYD No 05 -2
22.7.2009
Materials/ Products tested
Types of test/ Properties measured
Applied Methods/Techniques used
9. Eggs
Determination of sulfonamide residues (8):
Sulfadiazine, sulfathiazole, sulfamerazine,
sulfamethoxypyridazine, sulfamethazine,
sulfachlorpyridazine, sulfadoxine,
sulfadimethoxine
In house method using High
Performance Liquid Chromatography
– Tandem Mass Spectrometry (LCMS) (ESI-positive mode), validated
according to the requirements of the
Decision EU/657/2002.
10. Animal tissues, urine,
water
Determination of β-antagonists residues
(17): zilpaterol, terbutalin, salbutamol,
cimaterol, cibuterol, fenoterol, ritodrin,
clencyclohexerol, clenproperol, clenbuterol,
ractopamin, isoxsuprin, tulobuterol,
clenpenterol, mabuterol, mapenterol,
brombuterol
In house method based on the
corresponding method of the
European Community Reference
Laboratory AFSSA using High
Performance Liquid Chromatography
– Tandem Mass Spectrometry (LCMS/MS) (ESI-positive mode),
validated according to the
requirements of the Decision
EU/657/2002.
11. Animal tissues
Determination of tranquilizers (antianxiety
drugs) residues (7): acetopromazine,
chloropromazine, xylazine, promazine,
propionylopromazine, carazolol, azaperone
In house method using High
Performance Liquid Chromatography
– Tandem Mass Spectrometry (LCMS/MS), validated according to the
requirements of the Decision
EU/657/2002.
12. Animal urine
Detection and determination of (6)
antithyroid drugs (thiouracil, methyl
thiouracil, propyl thiouracil, phenyl
thiouracil, tapazol, mercaptobenzimidazol)
Microbiological tests
1.Foods for human
consumption
Page 3 of 4
1. Detection of Listeria monocytogenes
ISO 11290-1:1996/Αmd1:2004
2. Detection of Salmonella spp
ISO 6579:2002
3. Detection Vibrio parahaemolyticus
ISO 21872-1: 2007
4. Detection of the thermotolerant
Campylobacter spp
ISO 10272-1:2006
5. Enumeration of coagulase-positive
staphylococci
ISO 6888-2:1999
6. Enumeration of microorganisms at 30 οC
ISO 4833:2003
7. Enumeration of Escherichia coli
ISO 16649-2:2001
8. Enumeration of Enterobacteriaceae
ISO 21528-1:2004
9.Enumeration of Escherichia coli in live
bivalve molluscs
ISO 16649-3:2005
10.Enumeration of Listeria monocytogenes
ISO 11290-2:1998/Αmd1:2004
Annex F1/3 to the Certificate ESYD No 05 -2
22.7.2009
Materials/ Products tested
Types of test/ Properties measured
Applied Methods/Techniques used
1. Count of yeast and moulds at 25 οC
ISO 6611:2004
2. Detection of staphylococcal enterotoxins
type SEA to SEE
European screening method of the
CRL “Coagulase positive
staphylococci including
Staphylococcus aureus” Ver. 2,
01/04/2008
2. Milk and milk products
Site of assessment: Permanent laboratory premises, 25 Neapoleos str., Agia Paraskevi Attiki.
Approved signatories: G. Methenitou, Th. Alexandropoulos, K. Hatziagelou,
S. Karageorgis, I. Tomazinakis, A. Ntala, N. Vasiliadi, A.Choulidou, D.Milionis,
S.Samartzi, D.Skafidas, E. Katsarou, V. Georgakilas
This scope of Accreditation replaces the previous one dated 14.5.2009 and 27.3.2008.
The Accreditation Certificate No. 05-2, to ELOT EN ISO/IEC 17025:2005, is valid until 5.9.2012
Αthens, 22 July 2009
Rrof. Nikolaos D. Stathopoulos
Chairman of ESYD
Page 4 of 4
Annex F1/3 to the Certificate ESYD No 05 -2
22.7.2009