. Program Overview Christian Holm Les Houches Summer School MPI für Polymerforschung Ackermannweg 10 D-55128 Mainz, Germany Analytical methods Statistical Mechanics of electrolytes and double layers. Integral equation theory and Poisson-Boltzmann theory of charged plates, membranes, colloids, polyelectrolytes. Field theoretical and scaling approaches to polyelectrolytes and polyampholytes. Molecular interactions between colloids, polyelectrolytes, charged lipids–DNA complexes, biological macromolecules and biological soft matter. Adsorption phenomena and soft matter in confined geometries. Condensation phenomena for DNA. Computational methods Introduction to molecular dynamics of systems with long-range interaction potentials. Introduction to Monte–Carlo simulations of charged colloids and polymers in confined geometries. Statistical mechanics of various ensembles. Integrators, Langevin dynamics. Treatment of long-range interactions in periodic boundary conditions, reaction field methods, Ewald sums, Fast Fourier Transformation accelerated methods (P3M). Comparison of Poisson-Boltzmann and Debye-Hückel approximations, electrostatic stiffness, membrane models. Experimental methods Direct force measurements: comparison between osmotic stress, surface force apparatus, and atomic force microscopy. Non specific interactions (dispersion and electrical double layer interactions): DLVO and beyond (ion-ion correlation, etc). Specific interactions (directional due to hydration of the surfaces, presence of multipoles, hydrogen bonds, or due to complexes by coordination, chemical or structural inhomogeneities). Other methods of experimental determination of interactions (video microscopy, fluorescent microscopy): charged colloidal systems in confined geometries, electrostatic stabilized lipid– DNA complexes, condensation and interactions of single DNA chains and molecular assemblies of DNA.. Scattering experiments in colloidal dispersions, membranes, and polyelectrolyte solutions. Polyelectrolytes of various architectures (linear, rings, stars), and the more specific case of the hydrophobic polyelectrolytes. New advances in scattering experiments: use of microbeams, photon correlation spectroscopy (speckles), neutron spin echo, real-time scattering experiments (phase transitions). Coupling with other techniques (temperature and pressure jumps, calorimetry, force measurements). Advanced Study Institute Euro Summer School Electrostatic Effects in Soft Matter and Biophysics October 1- 13, 2000 in Les Houches, France