Experiments in Biochemistry/Molecular Biology
I. Techniques and Procedures
Chromatography
A laboratory experiment to demonstrate the principles of bio-ligand affinity
chromatography: the purification of chicken muscle AMP aminohydrolase
by phosphocellulose chromatography, M. Hahn and H. Husic, Biochem.
Educ. 17, 94-96 (1989).
Demonstration of chromatographic techniques using minicolumns, D. Miller et
al., Biochem. Educ. 19, 33-34 (1991).
Separation utilizing gel filtration and ion-exchange chromatography, S. Russo
and A. Radcliffe, J. Chem. Educ. 68, 168-171 (1991).
An inexpensive gel-filtration chromatography experiment, H. Rowe, J. Chem.
Educ. 70, 415-417 (1993).
Isolation of lactoferrin by immobilized metal ion affinity chromatography
(IMAC), A. Calvo and F. Batista-Viere, Biochem. Educ. 22, 50-52 (1994).
Rapid microscale isolation and purification of yeast alcohol dehydrogenase
using Cibacron blue affinity chromatography, C. Morgan and N. Moir, J.
Chem. Educ. 73, 1040-1041 (1996).
A Problem-solving approach to chromatography in the biochemistry lab,
F. Gorga, J. Chem. Educ. 77, 264-266 (2000).
Chromatofocusing, T. Makovec, Biochem. Mol. Biol. Educ. 28, 203-206
(2000).
Survey of biochemical separation techniques, M. Nilsson, J. Chem. Educ.
84,112-115 (2007).
Chromatographic separation of two proteins, J. Szeberenyi, Biochem. Mol.
Biol. Educ. 35, 71-72 (2007).
HPLC determination of caffeine and paraxanthine in urine: An assay for
cytochrome P450 1A2 activity, L. Furge and K. Fletke, Biochem. Mol. Biol.
Educ. 35, 138-144 (2007).
1
Computational Biochemistry
ENZYPLOT: a microcomputer assisted program for teaching enzyme
kinetics, F. Leone et al., Biochem. Educ. 23, 35-37 (1995).
Molecular modeling and experimental verification of the relative stability of
DNA base pairs: a biochemistry laboratory experiment, S. Burgess et al.,
Biochem. Educ. 24, 230-231 (1996).
Structural analysis and modeling of proteins on the web, D. Leon, S.
Uridil, and J. Miranda, J. Chem. Educ. 75, 731-734 (1998).
Teaching Bioinformatics, H. Salter, Biochem. Educ. 26, 3-10 (1998).
Using the computer in biochemical research, R. Boyer, Modern Experimental
Biochemistry, 3rd ed., p. 211-225 (2000), Benjamin Cummings (San Francisco).
Integrating computational chemistry into a project-oriented biochemistry
laboratory experience: a new twist on the lysozyme experiment. R. Peterson,
J. Cox, J. Chem. Educ. 78, 1551-1555 (2001).
Integrating Internet assignments into a Biochemistry/Molecular Biology
laboratory course, R. Kaspar, Biochem. Mol. Biol. Educ. 30, 36-39 (2002).
Introduction to interpretation of stochastic parameters: computer-intensive
procedures for evaluation of data in enzyme kinetics, K. Tenekedjiev and
K. Kolev, Biochem. Mol. Biol. Educ. 30, 414-418 (2002).
BioMoleculesAlive.org: The biochemistry and molecular biology digital
library update, P. Craig, Biochem. Mol. Biol. Educ. 31, 151-152 (2003).
Photochemical oxidation of bilirubin to biliverdin, W. Coleman, J. Chem.
Educ. 83, 1329-1330 (2006).
Utilization of integrated Michaelis-Menten equation to determine kinetic
constants, R. Bezerra and A. Dias, Biochem. Mol. Biol. Educ. 35, 145-150
(2007).
Molecular models of DNA, W. Coleman, J. Chem. Educ. 84, 809-810
(2007).
Molecular models of resveratrol, W. Coleman, J. Chem. Educ. 84, 11611162 (2007).
Lysozyme thermal denaturation and self-interaction, J. Schwinefus et al.,
J. Chem. Educ. 85, 117-120 (2008).
2
Vertical and horizontal integration of bioinformatics education, L. Furge et al.,
Biochem. Mol. Biol. Educ. 37, 26-36 (2009).
Electrophoresis
SDS-agarose gel electrophoresis in a simple procedure for determining high
molecular weight protein oligomerization, M. Brownleader et al., Biochem.
Educ. 22, 155-158 (1994).
Capillary electrophoresis: a fast and simple method for the determination of
the amino acid composition of proteins, P. Weber and D. Buck, J. Chem.
Educ. 71, 609-611 (1994).
An inexpensive agarose gel electrophoresis system, D. Betsch and J. Beard,
Biochem. Educ. 27, 48-50 (1999).
Electrophoretic transfer technique to detect and identify amylases in
polyacrylamide gels, V. Mulimani and S. Thippeswamy, Biochem. Mol.
Biol. Educ. 29, 250-254 (2001).
Determination of a diffusion coefficient by capillary electrophoresis, K.
Williams, B. Adhyaru, I. German, and T. Russell, J. Chem. Educ. 79, 14751476 (2002).
Agarose gel electrophoresis system in the classroom. J. De Mattos et al.
Biochem. Mol. Biol. Educ. 32, 254-257 (2004).
An SDS-PAGE examination of protein quaternary structure. J. Powers et al.
J. Chem. Educ. 82, 93-95 (2005).
Determination of the subunit molecular mass and composition of alcohol
dehydrogenase by SDS-PAGE, B. Nash, J. Chem. Educ. 84, 1508-1511
(2007).
Metal-catalyzed cleavage of tRNA-Phe, S. Kirk et al., J. Chem. Educ. 85,
676-678 (2008).
General Techniques (solutions, statistics, etc.)
Preparing solutions in the biochemistry lab, J. Risley, J. Chem. Educ.
68, 1054-1055 (1991).
3
Automated data collection from manual Hitachi model U-1100 spectrophotometers and analysis by means of Excel spreadsheets, D. Titheridge et al.,
Biochem. Educ. 23, 91-96 (1995).
Concepts and skills in the biochemistry/molecular biology lab, R. Boyer,
Biochem. Mol. Biol. Educ. 31, 102-105 (2003).
Teaching undergraduates to write scientific papers, L. Tomaska, ASBMB
Today, May, 20-21 (2007).
Ligand Binding
Spectrophotometric determination of the binding constants of succinate and
chloride to glutamic oxalacetic transaminase, A. Parody-Morreale et al., J.
Chem. Educ. 67, 988-991 (1990).
The binding of coomassie brilliant blue to bovine serum albumin, J. Sohl
and A. Splittgerber, J. Chem. Educ. 68, 262-264 (1991).
Evaluation of the Hill coefficient from Scatchard and Klotz plots, A. Sabouri
and A. Moosavi-Movahedi, Biochem. Educ. 22, 48-49 (1994).
Fluorescence measurements of ethidium binding to DNA, K. Strothkamp
and R. Strothkamp, J. Chem. Educ. 71, 77-81 (1994).
Analysis of receptor-ligand interactions, A. Attie and R. Raines, J. Chem.
Educ. 72, 119-121 (1995).
A ligand-binding study based on a protein assay method, H. Chial et al., J.
Chem. Educ. 72, 76-78 (1995).
Binding of bilirubin to goat serum albumin: determination of binding
constants, S. Tayyab and V. Trivedi, Biochem. Educ. 23, 98-100 (1995).
The shapes of Scatchard plots for systems with two sets of binding sites, A.
Bordbar et al., Biochem. Educ. 24, 172-175 (1996).
Fluorescent measurement of TNS binding to calmodulin, J. Buccigross et
al., J. Chem. Educ. 73, 275-278 (1996).
Binding of proflavin to chymotrypsin, H. Hendrickson, Biochem. Educ.
27, 118-121 (1999).
Study of protein-ligand Binding by fluorescence, M. Moller and A.
Denicola, Biochem. Mol. Biol. Educ., 30, 309-312 (2002).
4
Teaching receptor theory to biochemistry undergraduates, M. BenoreParsons and K. Sufka, Biochem. Mol. Biol. Educ. 31, 85-92 (2003).
Equilibrium gel filtration chromatography for the measurement of proteinligand binding in the undergraduate biochemistry laboratory. D. Craig, J. Chem.
Educ. 82, 96-98 (2005).
Quantitative determination of DNA-ligand binding using fluorescence
spectroscopy, E. Healy, J. Chem. Educ. 84, 1304-1307 (2007).
Molecular models of DAPI, W. Coleman, J. Chem. Educ. 84, 1307 (2007).
Steady-state fluorescence anisotropy to investigate flavonoids binding to
proteins, C. Ingersoll and C. Strollo, J. Chem. Educ. 84, 1313-1316 (2007).
UV thermal melting curves of tRNA-Phe in the presence of ligands, S. Kirk
et al., J. Chem. Educ. 85, 674-676 (2008
Spectroscopy
Fluorescence quenching of albumin. A spectrofluorimetric experiment, M.
Montero et al. Biochem Educ. 18, 99-101 (1990).
Using high field NMR to determine dehydrogenase stereospecificity with
respect to NADH, S. Mostad and A. Glasfeld, J. Chem. Educ. 70, 504-506
(1993).
NMR titration used to observe specific proton dissociation in polyprotic
tripeptides, J. Yarger, R. Nieman, and A. Bieber, J. Chem. Educ. 74, 243-245
(1997).
Resonance energy transfer in peptides: A biophysical laboratory experiment, A.
Hundzinski and B. Anderson, J. Chem. Educ. 76, 416-418(1999).
Proton NMR Studies of the conformation of an octapeptide, A. Rehart and J.
Gerig, J. Chem. Educ. 77, 892-895 (2000).
Carboxyfluorescin fluorescence experiments, S, Massou, R. Albigot, and
M. Prats, Biochem. Educ. 28, 171-173 (2000).
Protein tryptophan accessibility studied by fluorescence quenching, M.
Moller and A. Denicola, Biochem. Mol. Biol. Educ. 30, 175-178 (2002).
The free energy of denaturation of lysozyme: an undergraduate experiment
in biophysical chemistry, W. Kurtin and J. Lee, Biochem. Mol. Biol. Educ.
30, 244-247 (2002).
5
Labeling of Proteins with Fluorescent Probes, V. Levi and F. Flecha,
Biochem. Mol. Biol. Educ. 31, 333-336 (2003).
Investigation of model cell membranes with Raman spectroscopy, N.
Craig, W. Fuchsman, and N. Lacuesta, J. Chem. Educ. 80, 1282-1288
(2003).
Fundamentals of biomolecule analysis by electrospray ionization mass
spectrometry. A. Weinecke and V. Ryzhov, J. Chem. Educ. 82, 99-102.
A rapid method for the determination of the percentage of aliphatic versus
vinyl hydrogen atoms and levels of trans fatty acids in triacylglycerols, M.
Mosher et al., The Chemical Educator, 11, 1-3 (2006).
Spectroscopic measurement of the redox potential of cytochrome c for the
undergraduate biochemistry laboratory, D. Craig and E. Nichols, J. Chem.
Educ. 83,1325-1327 (2006).
Accessible NMR experiments studying the hydrodynamics of 15-Nenriched ubiquitin at low fields, L. Thompson and D. Rovnyak, Biochem.
Mol. Biol. Educ. 35, 49-56 (2007).
Surface plasmon resonance label-free monitoring of antibody antigen
interactions in real time, A. Kausaite et al., Biochem. Mol. Biol. Educ.
35, 57-63 (2007).
Complexation of copper (II) ion with tetraglycine as followed by electronic
absorption spectroscopy, E. Garribba and G. Micera, J. Chem. Educ. 84,
832-835 (2007).
Quantitative measurement of trans-fats by infrared spectroscopy, E.
Walker et al., J. Chem. Educ. 84, 1162-1165 (2007).
Absorption and scattering coefficients: a biophysical-chemistry experiment
using reflectance spectroscopy, G. Cordon and M. Lagorio, J. Chem.
Educ. 84, 1167-1171 (2007).
Quantitative determination of DNA-ligand binding using fluorescence
spectroscopy, E. Healy, J. Chem. Educ. 84, 1304-1307 (2007).
Molecular models of DAPI, W. Coleman, J. Chem. Educ. 84, 1307 (2007).
Steady-state fluorescence anisotropy to investigate flavonoids binding to
proteins, C. Ingersoll and C. Strollo, J. Chem. Educ. 84, 1313-1316 (2007).
6
A project-based biochemistry laboratory promoting the understanding and
uses of fluorescence spectroscopy in the study of biomolecular structures
and interactions, N. Briese and H. Jakubowski, Biochem. Mol. Biol.
Educ. 35, 272-279 (2007).
Lactate dehydrogenase kinetics and inhibition using a microplate reader,
J. Powers et al., Biochem. Mol. Biol. Educ. 35, 287-292 (2007).
An improved method for studying the enzyme-catalyzed oxidation of
glucose using luminescent probes, W. Bare et al., J. Chem. Educ. 84,
1511-1515 (2007).
Incorporating biological mass spectrometry into undergraduate teaching
labs, Part I: identifying proteins based on molecular mass, I. Arnquist and
D. Beussman, J. Chem. Educ. 84, 1971-1974 (2007).
Lysozyme thermal denaturation and self-interaction, J. Schwinefus et al.,
J. Chem. Educ. 85, 117-120 (2008).
Fluorescence spectroscopy of tRNA-Phe Y base in the presence of Mg(2+)
and small ligand molecules, S. Kirk et al., J. Chem. Educ. 85, 678-680 (2008).
Using FRET to measure the angle at which a protein bends DNA, J. Kugel,
Biochem. Mol. Biol. Educ. 36, 541-546 (2008).
Identifying gel-separated proteins using in-gel digestion, mass spectrometry,
and database searching, D. Beussman et al., Biochem. Mol. Biol. Educ. 37,
49-55 (2009).
II. Isolation and Analysis of Biomolecules
Amino acids/peptides/proteins/antibodies
Application of gel filtration for fractionation and molecular weight determination
of proteins, O. Malhotra and A. Kumar, Biochem. Educ. 17, 148-150 (1989).
A review of enzyme-immunoassay and a description of a competitive
enzyme-linked immunosorbent assay for the detection of immunoglobulin
concentrations, R. O’Kennedy et al., Biochem. Educ. 18, 136-140 (1990).
Isolation of the active site of cytochrome c by reverse-phase high
performance liquid chromatography, P. Kenigsberg et al. , J. Chem. Educ. 67,
177-179 (1990).
7
Western blotting with a concanavalin A-horseradish conjugate, E. Snyder
and R. Fall, Biochem. Educ. 18, 147-148 (1990).
Insulin: HPLC mapping of protease digestion products, M. Vesting, J. Chem.
Educ. 68, 958-960 (1991).
Isolation and characterization of bovine milk α-lactalbumin by IMAC, R. Boyer,
J. Chem. Educ. 68, 430-432 (1991), and Modern Experimental Biochemistry,
3rd ed. p. 257-278, (2000), Benjamin Cummings (San Francisco).
Identification of amino acids in unknown dipeptides: a derivatization with
9-fluorenylmethyl chloroformate and HPLC, C. Clapp et al., J. Chem. Edu.
69, A122 (1992).
Determination of distances between chromophores in proteins: a resonanceenergy transfer experiment, G. Gonzalez, Biochem. Educ. 22,150-151 (1994).
A fast and inexpensive western blot for the undergraduate laboratory, S.
Farrell and L. Farrell, J. Chem. Educ. 73, 740-744 (1995).
Derivation of the thermodynamic parameters involved in the elucidation of
protein thermal profiles, A. Saarboury and A. Moosavi-Movahedi, Biochem.
Educ. 23, 164-166 (1995).
Experimental determination of the free energy of unfolding of proteins,
S. Tayyab et al., Biochem. Educ. 23, 162-165 (1995).
Use of monoclonal antibodies in ELISA assays, J. Johnson, et al., Biochem.
Educ. 24, 50-52 (1996).
Labeling histidines in cytochrome c, A. Bonser and O. Moe, J. Chem. Educ.
73, 794-797 (1996).
Isolation and purification of calmodulin from bovine brain, J. Buccigross et al.,
J. Chem. Educ. 73, 273-276 (1996).
Protein structure and chromatographic behavior: The separation and
characterization of four proteins using gel filtration and ion-exchange
chromatography and gel electrophoresis, M. Chakravarthy, L. Snyder, T.
Vanyo, J. Holbrook, and H. Jakubowski, J. Chem. Educ. 73, 268-272 (1996).
An experiment on the succinylation of wheat gluten and its consequences for
pH-solubility, D. Fouques and M-C Ralet, Biochem. Educ. 24, 232-233 (1996).
Purification of horse heart cyt c by ion exchange, P. Mabrouk, J. Chem. Educ.
73, A149 (1996).
8
Microburger biochemistry: extraction and spectral characterization of
myoglobin from hamburger, S. Bylkas and L. Andersson, J. Chem. Educ. 74,
426-429 (1997).
An introduction to research in protein folding for undergraduates, C.
Jones, J, Chem. Educ. 74, 1306-1310 (1997).
Simulated western blot for the science curriculum, P. Cummings, Biochem.
Educ. 25, 39-40 (1997).
A simple immunoassay-based system capable of detecting antibody raised
against human IgG, G. Walsh et al., Biochem. Educ. 26, 157-160 (1998).
Enzyme-linked antibodies: A laboratory introduction to the ELISA, G.
Anderson and L. McNellis, J. Chem. Educ. 75, 1275-1278 (1998).
Isolation of gamma-globulin (IgG) from serum: a task that encourages
students to consolidate concepts on protein structure and properties, A.
Assis-Pandochi et al., Biochem. Educ. 26, 63-65 (1998).
Biochemical characterization of transducin, the G protein of bovine retina,
J. Muschietti et al., Biochem. Educ. 26, 77-81 (1998).
Investigating lipoproteins, M. Gillett, Biochem. Educ. 27, 51-54 (1999).
Green Fluorescent Protein Isoforms: Exercises to integrate molecular biology,
biochemistry, and biophysical chemistry, B. Hicks, J. Chem. Educ. 76, 409416 (1999).
A convenient and highly specific western blot experiment for introductory
biochemistry, C. Fenk, S. Grooms, and D. Gerbig, J. Chem. Educ. 77, 373375 (2000).
A simple method for immunodetection of membrane-associated proteins,
K. Daghastanli, R. Ferreira, G. Thedei, and P. Ciancaglini, Biochem. Mol.
Biol. 28, 256-260 (2000).
Identification of serum glycoproteins by SDS-PAGE and western blotting,
R. Boyer, Modern Experimental Biochemistry, 3rd ed., p. 321-332 (2000)
Benjamin Cummings (San Francisco).
A practical approach to the choice of a suitable detergent and optimal
conditions for solubilizing a membrane protein, H. Santos and P. Ciancaglini,
Biochem. Educ. 28, 178-182 (2000).
9
Complete Analysis of a Biologically Active Tetrapeptide: A project utilizing
thin-layer chromatography and tandem quadrupole mass spectrometry,
J. LeFevre and D. Dodsworth, J. Chem. Educ. 77, 503-505 (2000).
A Biochemical Study of Noncovalent Forces in Proteins using Phycocyanin
from Spirulina, B. Heller and Y. Gindt, J. Chem. Educ. 77, 1458-1460 (2000).
Structural analysis of a dipeptide, R. Boyer, Modern Experimental Biochemistry, 3rd ed. p. 227-242, (2000), Benjamin Cummings (San Francisco).
Structural Studies of Phycobiliproteins from Spirulina: Combining
spectroscopy, thermodynamics, and molecular modeling in an undergraduate
biochemistry experiment, A. Taylor and S. Feller, J. Chem. Educ. 79, 14671470 (2002).
Assessment of the purification of a protein by ion exchange and gel
permeation chromatography, M. Pugh and E. Schultz, Biochem. Mol. Biol.
Educ. 30, 179-183 (2002).
Identifying a Protein by MALDI-TOF Mass Spectrometry, A. Counterman,
M. Thompson, and D. Clemmer, J. Chem. Educ. 80, 177-180 (2003).
Preparing undergraduates to participate in the post-genome era: A capstone
laboratory experience in proteomics, E. Eberhardt, Biochem. Mol. Biol. Educ.
31, 402-409 (2003).
Bringing proteomics into the lab, F. Carvalho and M. Gillespie, Biochem. Mol.
Biol. Educ. 31, 46-51 (2003).
Teaching proteomics, L. Plesniak and E. Bell, Biochem. Mol. Biol. Educ. 31,
127-130 (2003).
Purification of colored photosynthetic proteins for understanding protein
isolation principles, M. Bes, J. Sancho, M. Peleato, M. Medina, C. GomezMoreno, and M. Fillat, Biochem. Mol. Biol. Educ. 31, 119-122 (2003).
Isolation of chicken immunoglobulins (IgY) from egg yolk, J. Goldring and
T. Coetzer, Biochem. Mol. Biol. Educ. 31, 185-187 (2003).
Salt fractionation of plasma proteins: a procedure to teach principles of
protein chemistry, A. Spadaro, A. Assis-Pandochi, Y. Lucisano-Valim,
and Z. Rothschild, Biochem. Mol. Biol. Educ. 31, 249-252 (2003).
Cytochrome c: A biochemistry laboratory course, J. Vincent and S. Woski,
J. Chem. Educ. 82, 1211-1214 (2005).
10
Spectroscopic measurement of the redox potential of cytochrome c for the
undergraduate biochemistry laboratory, D. Craig and E. Nichols, J. Chem.
Educ. 83,1325-1327 (2006).
Survey of biochemical separation techniques, M. Nilsson, J. Chem. Educ.
84,112-115 (2007).
Accessible NMR experiments studying the hydrodynamics of 15-Nenriched ubiquitin at low fields, L. Thompson and D. Rovnyak, Biochem.
Mol. Biol. Educ. 35, 49-56 (2007).
Surface plasmon resonance label-free monitoring of antibody antigen
interactions in real time, A. Kausaite et al., Biochem. Mol. Biol. Educ. 35,
57-63 (2007).
Chromatographic separation of two proteins, J. Szeberenyi, Biochem. Mol.
Biol. Educ. 35, 71-72 (2007).
Western blot analysis to illustrate relative control levels of the lac and ara
promoters in E. coli, B. Nielsen et al., Biochem. Mol. Biol. Educ. 35, 133137 (2007).
Steady-state fluorescence anisotropy to investigate flavonoids binding to
proteins, C. Ingersoll and C. Strollo, J. Chem. Educ. 84, 1313-1316 (2007).
A project-based biochemistry laboratory promoting the understanding and
uses of fluorescence spectroscopy in the study of biomolecular structures
and interactions, N. Briese and H. Jakubowski, Biochem. Mol. Biol.
Educ. 35, 272-279 (2007).
Determination of the subunit molecular mass and composition of alcohol
dehydrogenase by SDS-PAGE, B. Nash, J. Chem. Educ. 84, 1508-1511
(2007).
Incorporating biological mass spectrometry into undergraduate teaching
labs, Part I: identifying proteins based on molecular mass, I. Arnquist and
D. Beussman, J. Chem. Educ. 84, 1971-1974 (2007).
Lysozyme thermal denaturation and self-interaction, J. Schwinefus et al.,
J. Chem. Educ. 85, 117-120 (2008).
An integrated protein chemistry laboratory: Chlorophyll and chlorophyllase
K. Arkus and J. Jez, Biochem. Mol. Biol. Educ. 36, 125-128 (2008).
11
Teaching protein purification and characterization techniques: A studentinitiated, project-oriented biochemistry laboratory course, G. MacDonald,
J. Chem. Educ. 85, 1250-1253 (2008).
Identifying gel-separated proteins using in-gel digestion, mass spectrometry,
and database searching, D. Beussman et al., Biochem. Mol. Biol. Educ. 37,
49-55 (2009).
Carbohydrates
Changes in carbohydrate content during fruit ripening—a new approach of
teaching carbohydrate chemistry in biochemistry course, P. Chaimanee
and O. Suntornwat, Biochem. Educ. 22, 101-102 (1994).
Guidelines for determining the glycosylation characteristics of glycoconjugates
by simple procedures, J. Cabezas, Biochem. Educ. 23, 168-170 (1995).
Change in carbohydrate content during chickpea cooking: a new approach
of teaching of carbohydrate chemistry in biochemistry course, V. Mulimani,
S. Thippeswamy and Ramalingam, Biochem. Educ. 25, 44-45 (1997).
Carbohydrate Analysis: Can we control the ripening of bananas?, S. Deal,
C. Farmer, and P. Cerpovicz, J. Chem. Educ. 79, 479-480 (2002).
Lipids
Isolation and spectrophotometric characterization of photosynthetic
pigments, R. Boyer, Biochem. Educ. 18, 203-206 (1990), and Modern
Experimental Biochemistry, 3rd ed., p. 333-344, (2000) Benjamin Cummings.
(San Francisco).
Erythrocyte storage as a model for lipid peroxidation studies. An experimental assay for students of biochemistry and molecular biology, M.
Llanillo et al., Biochem. Educ. 22, 158-161 (1994).
Purification and characterization of triacylglycerols in natural oils, R. Boyer,
Modern Experimental Biochemistry, 3rd ed. p. 303-320 (2000), Benjamin
Cummings (San Francisco).
Determination of the Fatty Acid Content of Biological Membranes: A highly
versatile GC-MS Experiment, E. Schultz and M. Pugh, J. Chem. Educ. 78,
944-946 (2001).
12
Differential scanning calorimetric study of bilayer membrane phase
transitions, S. Ohline, M. Campbell, M. Turnbill, and S. Kohler, J. Chem.
Educ. 78, 1251-1257 (2001).
An improved method for the extraction and thin-layer chromatography of
chlorophyll a and b from spinach. H. Quach, R. Steeper, and G. Griffin, J.
Chem. Educ. 81, 385-387 (2004).
A rapid method for the determination of the percentage of aliphatic versus
vinyl hydrogen atoms and levels of trans fatty acids in triacylglycerols, M.
Mosher et al., The Chemical Educator, 11, 1-3 (2006).
Quantitative measurement of trans-fats by infrared spectroscopy, E.
Walker et al., J. Chem. Educ. 84, 1162-1165 (2007).
Molecular models of resveratrol, W. Coleman, J. Chem. Educ. 84, 11611162 (2007).
A project-based biochemistry laboratory promoting the understanding and
uses of fluorescence spectroscopy in the study of biomolecular structures
and interactions, N. Briese and H. Jakubowski, Biochem. Mol. Biol.
Educ. 35, 272-279 (2007).
III. Isolation and Characterization of Enzymes
Enzyme kinetics—the steady state observed, P. Buckley et al., Biochem.
Educ. 18, 101-102 (1990).
Determining the conformation of a ligand bound to an enzyme: application
of NMR spectroscopy in drug design, D. Craik et al., J. Chem. Educ. 68,
258-260 (1991).
An NMR study of the stereochemistry of the fumarase-catalyzed hydration
of fumaric acid, J. Olsen and R. Olsen, J. Chem. Educ. 68, 436-439 (1991).
Versatile partial purification of glucose 6-phosphate dehydrogenase by ionexchange chromatography, J. Keller et al., J. Chem. Educ. 68, 265-268
(1991).
A biochemistry project to study mushroom tyrosinase, M. Rodriquez and
W. Flurkey, J. Chem. Educ .69, 767-769 (1992).
Purification of lysozyme by linear salt gradient and SDS gel electrophoresis,
M. Hurst et al., J. Chem. Educ. 69, 850-852 (1992).
13
Probing denaturation by simultaneously monitoring residual enzyme activity
and intrinsic fluorescence, T. Silverstein and L. Blomberg, J. Chem. Educ.
69, 852-855 (1992).
Polyphenoloxidase: an enzyme widespread in fruits, S. Garcia and A.
Buzaleh, Biochem. Educ. 22, 152-153 (1994).
The hydrolysis of p-nitrophenyl acetate: a versatile reaction to study enzyme
kinetics, J. Anderson, J. Chem. Educ. 71, 715-717 (1994).
Testing the α-amylase inhibitor of the common bean, J. Moreno et al.,
J. Chem. Educ. 71, 350-352 (1994).
A kinetic study of yeast alcohol dehydrogenase, R. Utecht, J. Chem. Educ.
71, 436-438 (1994).
Designing a coupled assay system for aspartate aminotransferase, P. Arnold
and G. Parslow, Biochem. Educ. 23, 40-41 (1995).
A rapid and inexpensive procedure for the determination of proteolytic
activity, S. Castro and A. Cantera, Biochem. Educ. 23, 41-43 (1995).
Nucleophilic and enzymic catalysis of p-nitrophenylacetate hydrolysis, M.
Head et al., J. Chem. Educ. 72, 184-186 (1995).
A practical experiment on enzyme immobilization and characterization of the
immobilized derivatives, A. Manjon et al., Biochem. Educ. 23, 213-216 (1995).
An experiment on the chemical modification of essential tryptophan residues of barley
β-amylase, V. Mulimani and J. Lalitha, Biochem. Educ. 23, 218-219 (1995).
Kinetic study of the enzyme urease from Dolichos biflorus, K. Natarajan,
J. Chem. Educ. 72, 556-558 (1995).
Teaching biochemistry to wildlife management and oceanology students:
kinetics of LDH isozymes in brook charr, P. Rioux and P. Blier, Biochem.
Educ. 23, 38-39 (1995).
An assay procedure for determining the rate of an enzyme reaction lacking
an optical signal: validity of coupled enzyme assays, O. Malhotra et al.,
Biochem. Educ. 24, 56-59 (1996).
Zymography of extracellular matrix proteases, A. Quesada et al., Biochem.
Educ. 24, 170-171 (1996).
14
An experiment on the denaturation of α-chymotrypsin by an anionic surfactant, sodium dodecyl sulfate (SDS), V. Mulimani and J. Lalitha, Biochem.
Educ. 24, 52-54 (1996).
A rapid and inexpensive procedure for the determination of amylase activity,
V. Mulimani and J. Lalitha, Biochem. Educ. 24, 234-235 (1996).
Spectrofluorimetric visualization of the progress of an enzymatic reaction,
C. Saldanha and J. Martins-Silva, Biochem. Educ. 24, 235-236 (1996).
Regiospecific ester hydrolysis by orange peel esterase, T. Bugg, A. Lewin,
and E. Catlin, J. Chem. Educ. 74, 105-107 (1997).
Inhibition of cholinesterase by insecticides, L. Lai and J. Ho, Biochem. Educ.
25, 235-237 (1997).
The thermodynamic stability and catalytic activity of yeast alcohol dehydrogenase at different pH values, R. Tabor and J. Ladwig, Biochem. Educ. 25, 169170 (1997).
A simple laboratory experiment for the teaching of the assay and kinetic
characterization of enzymes, L. Vicario, D. Casti, and A. Iglesias, Biochem.
Educ. 25, 106-109 (1997).
A novel approach to practical enzymology teaching: a conductimetric
investigation of arginase, inorganic pyrophosphatase, aliphatic esterase,
ornithine carbamyl transferase, and arginosuccinate lyase activities from
mammalian liver, A. Lawrence et al., Biochem. Educ. 26, 56-62 (1998).
Immobilized lactase in the biochemistry laboratory, M. Allison and C. Bering,
J. Chem. Educ. 75, 1278-1281 (1998).
Purification of bovine carbonic anhydrase by affinity chromatography, C.
Bering and J. Kuhns, J. Chem. Educ. 75, 1021-1025 (1998).
A 19-F NMR study of enzyme activity, K. Peterman, K. Lentz, and J. Duncan,
J. Chem. Educ. 75, 1283-1285 (1998).
The competitive inhibition of yeast alcohol dehydrogenase by 2,2,2-trifluoroethanol, R. Tabor, Biochem. Educ. 26, 239-242 (1998).
Kinetics of Papain: An introductory biochemistry laboratory experiment, K.
Cornely, E. Crespo, M. Earley, R. Kloter, A. Levesque,and M. Pickering,
J. Chem. Educ. 76, 644-646 (1999).
15
Enzymic browning in potatoes: a simple assay for a polyphenol oxidase
catalyzed reaction, J. Busch, Biochem. Educ. 27, 171-173 (1999).
Kinetic analysis of tyrosinase, R. Boyer, Modern Experimental Biochemistry
3rd ed. p.279-302, (2000), Benjamin Cummings (San Francisco).
Chemical cross-linking analysis of calcium-ATPase from rabbit skeletal
muscle, B. Murray and K. Ohlendieck, Biochem. Educ. 28, 41-46 (2000).
Probing the active site of yeast alcohol dehydrogenase through microscale
yeast-mediated reductions of acetophenone and acetylpyridines, M. Lee
et al. J. Chem. Educ. 77, 363-366 (2000).
Demonstration of the principles of enzyme-catalyzed reactions using
alkaline phosphatase, N. Price and B. Newman, Biochem. Mol. Biol.
Educ. 28, 207-210 (2000).
The determination of chitinase activity of grapes, N. Byrne, M. Duxbury,
and N. Sharpe, Biochem. Mol. Biol. Educ. 29, 144-146 (2001).
Aminotransferase from insect cells, H. Erwin, G. de Ridder, and P. Barton,
Biochem. Mol. Biol. Educ. 29, 196-198 (2001).
A novel and innovative biochemistry laboratory: crystal growth of hen
egg white lysozyme, J. Chem. Educ. 79, 366-369 (2002).
Optimization of kinetic parameters of enzymes, R. Heinrich, E. MelendezHevia, and H. Cabezas, Biochem. Mol. Biol. Educ. 30, 184-188 (2002).
Kinetic studies with alkaline phosphatase in the presence and absence of
inhibitors and divalent cations, R. Dean, Biochem. Mol. Biol. Educ. 30, 401407 (2002).
Preparation, purification, and secondary structure determination of Bacillus
circulans xylanase. A modular laboratory incorporating aspects of
molecular biology, biochemistry, and biophysical chemistry, S. Russo and
L. Gentile, J. Chem. Educ. 83, 1850-1852 (2006).
HPLC determination of caffeine and paraxanthine in urine: An assay for
cytochrome P450 1A2 activity, L. Furge and K. Fletke, Biochem. Mol. Biol.
Educ. 35, 138-144 (2007).
Utilization of integrated Michaelis-Menten equation to determine kinetic
constants, R. Bezerra and A. Dias, Biochem. Mol. Biol. Educ. 35, 145-150
(2007).
16
From egg to crystal: a practical on purification, characterization, and
crystallization of lysozyme for bachelor students, V. Olieric et al. Biochem.
Mol. Biol. Educ. 35, 280-286 (2007).
Lactate dehydrogenase kinetics and inhibition using a microplate reader,
J. Powers et al. Biochem. Mol. Biol. Educ. 35, 287-292 (2007).
An improved method for studying the enzyme-catalyzed oxidation of
glucose using luminescent probes, W. Bare et al., J. Chem. Educ. 84,
1511-1515 (2007).
Immobilized -galactosidase in the biochemistry laboratory, V. Mulimani
and K. Dhananjay, J. Chem. Educ. 84, 1974-1976 (2007).
Thermal and chemical denaturation of Bacillus circulans xylanase, L. Gentile et
al., Biochem. Mol. Biol. Educ. 36, 428-432 (2008).
Red seaweed enzyme-catalyzed bromination of bromophenol red: An inquirybased kinetics laboratory experiment for undergraduates, P. Jittam et al., Biochem.
Mol. Biol. Educ. 37, 99-105 (2009).
IV. Metabolism/Regulation/Transport
The energetics of aerobic versus anaerobic respiration, T. Champion and
R. Schwenz, J. Chem. Educ. 67, 528-530 (1990).
Use of DCPIP in a colorimetric method to investigate electron transport in
crude heart mitochondrial extracts, A. Myers, Journal of Biol. Educ. 24, 123126 (1990).
Mitochondria from rat liver: method for rapid preparation and study, C. Heisler,
Biochem. Educ. 19, 35-38 (1991).
An experiment on glycogen biosynthesis in E. coli, A. Lodeiro et al, Biochem.
Educ. 22, 213-214 (1994).
Demonstration of the regulation of phosphorylase by reversible
phosphorylation and allosteric effectors, R. Toomik et al., Biochem. Educ. 22,
205-207 (1994).
An experiment illustrating catabolite repression in yeast, W. Baker, Biochem
Educ. 23, 216-217 (1995).
17
Light-induced proton transport through chloroplast membranes, J. Ho and E.
Po, Biochem. Educ. 24, 179-180 (1996).
Following glycolysis using 13-C NMR, T. Mega, C. Carlson, and D. Cleary,
J. Chem. Educ. 74, 1474-1476 (1997).
Some aspects of yeast anaerobic metabolism examined by the inhibition of
pyruvate decarboxylase, E. Martin, J. Chem. Educ. 75, 1281-1283 (1998).
Photosynthesis and respiration in leaf slices, S. Brown, Biochem. Educ. 26,
164-167 (1998).
An experiment illustrating metabolic regulation in situ using digitonin permeabolized yeast cells, A. Ponces Freire et al., Biochem. Educ. 26, 161-163
(1998).
A simple experiment demonstrating the allosteric regulation of yeast pyruvate
kinase, R. Taber, A. Campbell, and S. Spencer, Biochem. Educ. 26, 73-76
(1998).
An in Vivo 13-C NMR Analysis of the Anaerobic Yeast Metabolism of 1-13-C
Glucose, B. Giles, Z. Matsche, R. Egeland, R. Reed, S. Morioka, and R.
Taber, J. Chem. Educ. 76, 1564-1566 (1999).
A simple experiment illustrating metabolic regulation: induction versus
repression of yeast α-glucosidase, B. Stambuk, Biochem. Educ. 27, 177-180
(1999).
Isolation, subfractionation, and enzymatic analysis of beef heart mitochondria,
R. Boyer, Modern Experimental Biochemistry, 3rd ed. p. 357-370 (2000)
Benjamin Cummings (San Francisco).
The excellence of turnip mitochondrial fractions, J. Vicente and V. Madeira,
Biochem. Educ. 28, 104-106 (2000).
A simple laboratory exercise illustrating active transport in yeast cells,
B. Stambuk, Biochem. Mol. Biol. Educ. 28, 313-317 (2000).
Photoinduced proton transport through chloroplast membranes, R. Boyer,
Modern Experimental Biochemistry, 3rd ed. p. 345-356 (2000), Benjamin
Cummings (San Francisco).
Experimental treatment of the laws of heterogeneous catalysis with
immobilized yeast cells, P. Grunwald, Biochem. Educ. 28, 96-99 (2000).
18
Studying ion channels in undergraduate laboratories, A. Garrill, Biochem.
Mol. Biol. Educ. 28, 318-321 (2000).
A laboratory experiment demonstrating the dynamic character of membranes,
M. Pugh and E. Schultz, Biochem. Mol. Biol. Educ. 28, 322-326 (2000).
The pentose phosphate pathway in the yeasts Saccharomyces cerevisiae
and Kloeckera apiculata, an exercise in comparative metabolism for food
and wine science students, C. Steel, P. Grbin, and A. Nichol, Biochem.
Mol. Biol. Educ. 29, 245-249 (2001).
Designer metabolic pathways in the teaching laboratory, M. Gillett, Biochem.
Mol. Biol. Educ. 29, 147-151 (2001).
Kinetic analysis of glucose-6-phosphatase: an investigative approach to
carbohydrate metabolism, M. Wallert, J. Foster, D. Scholnick, A. Olmschenk,
B. Kuehn, and J. Provost, Biochem. Mol. Biol. Educ. 29, 199-203 (2001).
Nitrate reductase: A model system for the investigation of enzyme induction
in eukaryotes, C. Pike, W. Cohen, and J. Monroe, Biochem. Mol. Biol. Educ.
30, 111-116 (2002).
Transcriptional and posttranslational regulation of a membrane nutrient
transporter, B. Stambuk, Biochem. Mol. Biol. Educ. 30, 388-393 (2002).
Diphenyl carbazide restores electron transport in isolated, illuminated chloroplasts
after electron transport from water has been eliminated by mild heat treatment,
R. Dean and T. Pocock, Biochem. Mol. Biol. Educ. 32, 381-389 (2004).
Student-designed enzyme-linked metabolite assay kits. D. Hancock et al. Biochem.
Mol. Biol. Educ. 32, 326-330 (2004).
Photochemical oxidation of bilirubin to biliverdin, W. Coleman, J. Chem.
Educ. 83, 1329-1330 (2006).
Spectroscopic measurement of the redox potential of cytochrome c for the
undergraduate biochemistry laboratory, D. Craig and E. Nichols, J. Chem.
Educ. 83, 1325-1327 (2006).
Solar irradiation of bilirubin: An experiment in photochemical oxidation, A.
Pillay and F. Salih, J. Chem. Educ. 83, 1327-1329 (2006).
Western blot analysis to illustrate relative control levels of the lac and ara
promoters in E. coli, B. Nielsen et al., Biochem. Mol. Biol. Educ. 35, 133137 (2007).
19
HPLC determination of caffeine and paraxanthine in urine: An assay for
cytochrome P450 1A2 activity, L. Furge and K. Fletke, Biochem. Mol. Biol.
Educ. 35, 138-144 (2007).
V. Clinical/Nutritional
Determination of urinary ammonium: a practical class experiment designed
to introduce medical students to mechanisms of extracellular and intracellular pH homeostasis, C. Marinho and J. Barros, Biochem. Educ. 22,
215-216 (1994).
A practical approach to chemical sensors through potentiometric transducers:
determination of urea in serum by means of a biosensor, E. Martinez and
S. Alegret, J. Chem. Educ. 71, A67 (1994).
The role of molecular biology in characterization of α-thalassaemia,
J. Howarth et al., Biochem. Educ. 24, 59-61 (1996).
Use of blood-glucose test strips for introducing enzyme electrodes and modern
biosensors, J. Wang and C. Macca, J. Chem. Educ. 73, 794-797 (1996).
The analysis of riboflavin in urine using fluorescence, J. Henderleiter and R.
Hyslop, J. Chem. Educ. 73, 563-565 (1996).
Working with enzymes—where is lactose digested?, S. Pope, T. Tolleson,
R. Williams, R. Underhill, and R. Deal, J. Chem. Educ. 75, 761-762 (1998).
Measurement of cholesterol and vitamin C in biological samples, R. Boyer,
Modern Experimental Biochemistry, 3rd ed. p. 371-388 (2000), Benjamin
Cummings (San Francisco).
An experiment on the apoptosis induced by polyamine adducts produced
in the presence of serum amine oxidase, I. Fajardo, Biochem. Educ. 28,
110-112 (2000).
Glucocorticoid-induced apoptosis: a simple set of laboratory experiments,
G. Vicent, G. Lanuza, D. Romero, L. Di Croce, and A. Pecci, Biochem. Mol.
Biol. Educ. 28, 307-312 (2000).
From gene to protein: Prostatic acid phosphatase: Structure and expression of
gene and protein. P. Laidler et al., Biochem. Mol. Biol. Educ. 32: 400-409 (2004).
Testing for genetically modified foods using PCR. A. Taylor and S. Sajan, J. Chem.
Educ. 82, 597-599 (2005).
20
Photochemical oxidation of bilirubin to biliverdin, W. Coleman, J. Chem.
Educ. 83, 1329-1330 (2006).
Solar irradiation of bilirubin: An experiment in photochemical oxidation, A.
Pillay and F. Salih, J. Chem. Educ. 83, 1327-1329 (2006).
HPLC determination of caffeine and paraxanthine in urine: An assay for
cytochrome P450 1A2 activity, L. Furge and K. Fletke, Biochem. Mol. Biol.
Educ. 35, 138-144 (2007).
A laboratory exercise to determine human ABO blood type by noninvasive
methods, M. Martin and S. Detzel, Biochem. Mol. Biol. Educ. 36, 139-146
(2008).
VI. Molecular Biology (nucleic acids, cloning, techniques)
An introduction to restriction mapping of DNA, C. Hepfer and S. Turchi,
Biochem. Educ. 17, 48-50 (1989).
The purification and translation of globin mRNA, E. Wolf, Biochem. Educ.
17, 45-47 (1989).
Restriction enzyme mapping: a simple student practical, S. Higgins et al.,
Biochem. Educ. 18, 144-146 (1990).
Practical molecular biology for students: an integrated approach to teaching
basic techniques, B. Hames et al., Biochem. Educ. 18, 141-144 (1990).
You too can be a molecular biologist: a basic cloning experiment for the
undergraduate biochemistry laboratory, S. Farrell et al., J. Chem. Educ.
68, 707-709 (1991).
Field inversion agarose gel electrophoresis of DNA, D. Weller and P. Gariepy,
J. Chem. Educ. 68, 81-83 (1991).
Demonstration of DNA strand breakage induced by ultraviolet light: an
experiment to show molecular events in carcinogenesis, R. Russo and
J. Russo, J. Chem. Educ. 70, 330-333 (1993).
The polymerase chain reaction, W. Timmer et al., and J. Villalobos, J. Chem.
Educ. 70, 273-277 (1993).
PCR amplification of DNA, D. Weller, J. Chem. Educ. 71, 340-342 (1994).
21
Non-radioactive detection of the Staphylococcus aureus protein A gene using
the polymerase chain reaction (PCR), A. Corbett et al, Biochem. Educ. 22, 5255 (1994).
A fast restriction enzyme experiment for the undergraduate biochemistry
laboratory, S. Farrell, J. Chem. Educ. 71, 1095-1096 (1994).
A practical exercise involving RNA isolation, transfer, and hybridization,
M. Marano et al., Biochem. Educ. 22, 207-210 (1994).
DNA composition analysis by nuclease digestion and HPLC, S. Wietstock,
J. Chem. Educ. 72, 950-952 (1995).
An analysis of the involvement of purine ribonucleotides in eukaryotic
protein synthesis, T. Lawson, J. Chem. Educ. 72, 104-106 (1995).
Determination of the solution conformation of 3’-azido-3’deoxythymidine5’-monophosphate (AZTMP), M. Lee, J. Chem. Educ. 73, 184-186 (1996).
Research in undergraduate instruction: a biotech lab project for recombinant
protein expression in bacteria, M. Brockman, A. Ordman and A. Malcolm, J.
Chem. Educ. 73, 542-544 (1996).
A laboratory class experiment illustrating basic principles of DNA cloning
and molecular biology techniques, L. Perez-Pons and E. Querol, Biochem.
Educ. 24, 54-56 (1996).
Restriction mapping and gene analysis, M. Simsek, Biochem. Educ. 24,
117-119 (1996).
A fast CTAB method of human DNA isolation for polymerase chain reaction
applications, J. Thomas et al., Biochem. Educ. 25, 233-235 (1997).
Making your own gene library, J. Perez-Ortin et al., Biochem. Educ. 25,
237-241 (1997).
Molecular genetic diagnosis by the polymerase chain reaction, A. Beyer and
M. Varsanyi, Biochem. Educ. 25, 170-173 (1997).
Isolation of nuclear, chloroplast, and mitochondrial DNA from plants, J. Day
Biochem. Educ. 25, 41-43 (1997).
A demonstration of genomic DNA profiling by RAPD analysis, J. Hammond
and G. Spanswick, Biochem. Educ. 25, 109-111 (1997).
22
Detection of non-B-DNA secondary structures by S1 nuclease digestion,
M. del Olmo et al., J. Chem. Educ. 75, 762-764, (1998).
Synthesis and Purification of a Hammerhead Ribozyme and a FluoresceinLabeled RNA Substrate. A Biochemistry Laboratory: Part 1, C. Chow and
S. Somne, J. Chem. Educ. 76, 648-651 (1999).
Monitoring Hammerhead Ribozyme-Catalyzed Cleavage with a FluoresceinLabeled Substrate: Effects of magnesium ions and antibiotic inhibitors, C.
Chow, S. Somne, and B. zllano-Sotelo, J. Chem. Educ. 76, 651-653 (1999).
A Structure-Function Study of RecA, J. Gegner, N. Spruill, and L. Plesniak,
J. Chem. Educ. 76, 1562-1564 (1999).
RNA isolation from plant tissues, M. Claros and F. Canovas, Biochem. Educ.
27, 110-113 (1999).
An experiment illustrating DNA-protein interactions using nuclear extracts
from chicken erythrocytes, B. Garat, Biochem. Educ. 27, 232-236 (1999).
Chromatin Isolation and DNA Sequence Analysis in Large Undergraduate
Laboratory Sections, A. Hagerman, J. Chem. Educ. 76, 1426-1429 (1999).
A guided experimental approach to practical molecular pharmacology
teaching: demonstration of sequence selectivity of DNA-binding drugs
by arrested polymerase chain reaction, R. Gambari, Biochem. Educ.
28, 100-103 (2000).
DNA Topology Analysis in the Undergraduate Biochemistry Laboratory,
M. Keck, J. Chem. Educ. 77, 1471-1474 (2000).
Extraction and characterization of bacterial DNA, R. Boyer, Modern
Experimental Biochemistry, 3rd ed. p. 399-414 (2000), Benjamin Cummings
(San Francisco).
Plasmid DNA isolation and characterization by electrophoresis, R. Boyer,
Modern Experimental Biochemistry, 3rd ed. p. 415-430 (2000), Benjamin
Cummings (San Francisco).
The action of restriction endonucleases on plasmid or viral DNA, R. Boyer,
Modern Experimental Biochemistry, 3rd ed. p. 431-442 (2000), Benjamin
Cummings (San Francisco).
A laboratory exercise designed to teach medical students about reversetranscription polymerase chain reaction, M. Fay, N. Chandar, and S.
Viselli, Biochem. Mol. Biol. Educ. 29, 234-238 (2001).
23
Restriction enzyme mapping of bacterial urease genes: using degenerate
primers to expand experimental outcomes, K. Reed, Biochem. Mol. Biol.
Educ. 29, 239-244 (2001).
Biocatalytic Lactone Generation in Genetically Engineered E. coli and
Identification of Products by Gas Chromatography-Mass Spectroscopy,
C. Slawson, R. Potter, and J. Stewart, J. Chem. Educ. 78, 1533-1535,
(2001).
Identification of Yeast V-ATPase Mutants by Western Blots Analysis of
Whole Cell Lysates, K. Parra-Belky, J. Chem. Educ. 79, 1348-1351 (2002).
Detection of genetically modified organisms in food by DNA extraction and
PCR amplification, L. Thion, C. Vossen, B. Couderc, M. Erard, and B.
Clemencon, Biochem. Mol. Biol. Educ. 30, 51-55 (2002).
Introducing students to DNA, H. Streicher and A. Brodte, Biochem. Mol. Biol.
Educ. 30, 104-105 (2002).
Use of chloroplast rRNA gene to introduce basic molecular biology techniques,
B. Nielsen and S. Echols, Biochem. Mol. Biol. Educ. 30, 408-413 (2002).
Identification of Forensic Samples via Mitochondrial DNA in the Undergraduate
Biochemistry Laboratory, J. Millard and A. Pilon, J. Chem. Educ. 80, 444-446
(2003).
A novel, inexpensive, and highly sensitive experiment for demonstration of
DNA damage in human leukocytes by single cell electrophoresis (comet
assay), B. Manjunatha, G. Manjunatha, C. Sarika, K. Roopa, and G. Naik,
Biochem. Mol. Biol. Educ. 31, 34-36 (2003).
Crime scene investigations, K. Lounsbury, Biochem. Mol. Biol. Educ. 31,
37-41 (2003).
A laboratory exercise in comparative DNA analysis, L. Brown, Biochem.
Mol. Biol. Educ. 31, 177-179 (2003).
Photoinduced oxidative DNA damage revealed by an agarose gel nicking
assay, V. Shafirovich, C. Singh, and N. Geacintov, J. Chem. Educ. 80,
1297-1300 (2003).
Outcomes of a research-driven laboratory and literature course designed to
enhance undergraduate contributions to original research, M. Rasche, Biochem.
Mol. Biol. Educ. 32, 101-107 (2004).
24
Preparation, purification, and secondary structure determination of Bacillus
circulans xylanase. A modular laboratory incorporating aspects of
molecular biology, biochemistry, and biophysical chemistry, S. Russo and
L. Gentile, J. Chem. Educ. 83, 1850-1852 (2006).
Western blot analysis to illustrate relative control levels of the lac and ara
promoters in E. coli, B. Nielsen et al., Biochem. Mol. Biol. Educ. 35, 13337 (2007).
Molecular models of DNA, W. Coleman, J. Chem. Educ. 84, 809-810
(2007).
Using restriction mapping to teach basic skills in the molecular biology lab,
L. Walsh et al., Biochem. Mol. Biol. Educ. 35, 199-205 (2007).
Quantitative determination of DNA-ligand binding using fluorescence
spectroscopy, E. Healy, J. Chem. Educ. 84, 1304-1307 (2007).
Molecular models of DAPI, W. Coleman, J. Chem. Educ. 84, 1307 (2007).
A project-based biochemistry laboratory promoting the understanding and
uses of fluorescence spectroscopy in the study of biomolecular structures
and interactions, N. Briese and H. Jakubowski, Biochem. Mol. Biol.
Educ. 35, 272-279 (2007).
Probing changes in the conformation of tRNA-Phe: an integrated biochemistry
laboratory course, S. Kirk et al., J. Chem. Educ. 85, 666-674 (2008).
UV thermal melting curves of tRNA-Phe in the presence of ligands, S. Kirk
et al., J. Chem. Educ. 85, 674-676 (2008).
Metal-catalyzed cleavage of tRNA-Phe, S. Kirk et al., J. Chem. Educ. 85,
676-678 (2008).
Fluorescence spectroscopy of tRNA-Phe Y base in the presence of Mg (2+)
and small ligand molecules, S. Kirk et al., J. Chem. Educ. 85, 678-680 (2008).
A laboratory exercise to determine human ABO blood type by noninvasive
methods, M. Martin and S. Detzel, Biochem. Mol. Biol. Educ. 36, 139-146
(2008).
Using FRET to measure the angle at which a protein bends DNA, J. Kugel,
Biochem. Mol. Biol. Educ. 36, 541-546 (2008).
25
Caenorhabditis elegans as an undergraduate educational tool for teaching
RNAi, J. Andersen et al., Biochem. Mol. Biol. Educ. 36, 417-427 (2008).
A new way to introduce microarray technology in a lecture/laboratory setting by
studying the evolution of this modern technology, M. Rowland-Goldsmith, Biochem.
\Mol. Biol. Educ. 37, 37-43 (2009).
VII. Integrated Projects
Focusing on a single enzyme, J. Markwell, J. Chem. Educ. 70, 1018-1020 (1993).
An integrated approach to the undergraduate biochemistry laboratory, J.
Harman et al., J. Chem. Educ. 72, 641-643 (1995).
An integrated biochemistry laboratory including molecular modeling,
A. Wolfson, M. Hall, and T. Branham J. Chem. Educ. 73, 1026-1029 (1996).
“Research in Undergraduate Instruction “, M. Brockman, A. Ordman, and A.
Campbell, J. Chem. Educ. 73, 542-544 (1996).
Utilizing isolation, purification, and characterization of enzymes as projectoriented labs for undergraduate biochemistry, S. Deal and M. Hurst, J.
Chem. Educ. 74, 241-242 (1997).
A project-oriented biochemistry laboratory, P. Craig, J. Chem. Educ. 76,
1130-1135 (1999).
A single protein research integrated advanced biochemistry laboratory
course: spectroscopic determination of tyrosyl side chain pK, B. Blanchette
and B. Singh, Biochem. Educ. 28, 107-109 (2000).
Integrating computational chemistry into a project-oriented biochemistry
laboratory experience: a new twist on the lysozyme experiment. R. Peterson,
J. Cox, J. Chem. Educ. 78, 1551-1555 (2001).
The most proficient enzyme as the central theme in an integrated, researchbased biochemistry laboratory, J. Smiley, Biochem. Mol. Biol. Educ. 30, 4550 (2002).
Development of a biochemistry laboratory course with a project-oriented
goal, R. Stahelin, R. Forslund, D. Wink, and W. Cho, Biochem. Mol. Biol.
Educ. 31, 106-112 (2003).
26
Isolation, purification, and characterization of bovine heart and yeast
cytochromes c: An integrated biochemistry laboratory experience,
B. Hunnes, Biochem. Mol. Biol. Educ. 31, 242-248 (2003).
Preparing undergraduates to participate in the post-genome era: A
capstone laboratory experience in proteomics, E. Eberhardt et al.,
Biochem. Mol. Biol. Educ. 31, 402-409 (2003).
An undergraduate biochemistry laboratory course with an emphasis on a
research experience, M. Caspers and E. Roberts-Kirchhoff, Biochem. Mol.
Biol. Educ. 31, 303-307 (2003).
Advanced instrumentation projects for first-year biochemistry laboratory,
M. Hall, C. Guth, S. Kohler, and A. Wolfson, Biochem. Mol. Biol. Educ.
31, 115-118 (2003).
Gene Amplification by PCR and subcloning into a GFP-fusion plasmid expression
vector as a molecular biology laboratory course, J. Bornhorst, M. Deibel and
Mulnix, Biochem. Mol. Biol. Educ. 32, 173-182 (2004).
Development of green fluorescent protein-based laboratory curriculum,
P. Larkins and Y. Hartberg, Biochem. Mol. Biol. Educ. 33, 41-45 (2005).
Preparation, purification, and secondary structure determination of Bacillus
circulans xylanase. A modular laboratory incorporating aspects of molecular
biology, biochemistry, and biophysical chemistry, S. Russo and L. Gentile,
J. Chem. Educ. 83, 1850-1852 (2006).
Western blot analysis to illustrate relative control levels of the lac and ara
promoters in E. coli, B. Nielsen et al., Biochem. Mol. Biol. Educ. 35, 133137 (2007).
Using restriction mapping to teach basic skills in the molecular biology lab,
L. Walsh et al., Biochem. Mol. Biol. Educ. 35, 199-205 (2007).
A project-based biochemistry laboratory promoting the understanding and
uses of fluorescence spectroscopy in the study of biomolecular structures
and interactions, N. Briese and H. Jakubowski, Biochem. Mol. Biol.
Educ. 35, 272-279 (2007).
A streamlined molecular module for undergraduate biochemistry labs, G. Muth
and J. Chihade, Biochem. Mol. Biol. Educ. 36, 209-216 (2008).
Cloning yeast actin cDNA leads to an investigative approach for the molecular
biology laboratory, M. Black et al., Biochem. Mol. Biol. Educ. 36, 217-224 (2008).
27
Probing changes in the conformation of tRNA-Phe: an integrated biochemistry
laboratory course, S. Kirk et al., J. Chem. Educ. 85, 666-674 (2008).
Teaching protein purification and characterization techniques: A studentinitiated, project-oriented biochemistry laboratory course, G. MacDonald,
J. Chem. Educ. 85, 1250-1253 (2008).
Thermal and chemical denaturation of Bacillus circulans xylanase, L. Gentile et
al., Biochem. Mol. Biol. Educ. 36, 428-432 (2008).
Vertical and horizontal integration of bioinformatics education, L. Furge et al.,
Biochem. Mol. Biol. Educ. 37, 26-36 (2009).
RNase One gene isolation, expression, and affinity purification, C. Bailey,
Biochem. Mol. Biol. Educ. 37, 44-48 (2009).
From gene mutation to protein characterization, D. Moffet, Biochem. Mol. Biol.
Educ. 37, 110-115. (2009).
28