Exercise 24 and 26: Spinal Cord, Spinal Nerve, Reflexes, and the Autonomic Nervous System 1. Define the following terms, and identify them on a model with the gross structures of the spinal cord (posterior view) [e.g. big open torso model (T7 and T10); Somso model (M11)]: a. Conus medullaris – cone shaped region along the 1st or 2nd vertebra b. Cauda equina – collection of spinal nerves along the inferior end of the vertebral canal c. Filum terminale – fibrous extensions of the pia mater d. Cervical enlargement e. Lumbar enlargement f. Sympathetic chain ganglia g. Dorsal root ganglia and plexuses 2. Identify structures of the spinal cord on a model (e.g. M2, M3, M4: 3-D models of the spinal cord cross section; Somso Model (M11)) a. Gray matter i. Dorsal/posterior horns ii. Ventral/anterior horns iii. Lateral horn iv. Gray commissure v. Central canal vi. Dorsal/posterior root vii. Dorsal/posterior root ganglion viii. Dorsal/posterior ramus ix. Ventral/anterior root x. Ventral/anterior ramus xi. Rami communicantes (white ramus and gray ramus) xii. Spinal nerves b. White matter i. Posterior white column/funiculus 1 ii. Lateral white column/funiculus iii. Anterior white column/funiculus iv. Anterior median fissure v. Posterior median sulcus c. Meninges i. Epidural space ii. Dura mater iii. Subdural space iv. Arachnoid mater v. Subarachnoid space vi. Pia mater 3. Identify structures of the spinal cord on a histology slide a. Anterior funiculus, lateral funiculus, and posterior funiculus b. Dorsal horn, ventral horn, lateral horn c. Gray commissure d. Central canal lined by ependymal cells e. Anterior median fissure f. Posterior median sulcus Spinal nerves and spinal nerve plexuses [e.g. Head & Neck Model-M9, hanging skeletal/spinal cord model (MA); Somso Model (M11)] 1. Identify all 31 pairs of spinal nerves and indicate they are mixed (motor and sensory) nerves: 1st – 7th pair emerge above the vertebra they are named after, the rest emerge below the vertebra they are named after, except spinal nerve C8 emerges between the 7th cervical and the 1st thoracic vertebrae a. Cervical (C1-C8) b. Thoracic (T1-T12) c. Lumbar (L1-L5) d. Sacral (S1-S5) [S5 is missing on MA model] e. Coccygeal (Co1) [Co1 is missing on MA model] 2 2. Identify the location of the following spinal nerves and plexuses on a model, and give the function of each [e.g. “Nerve Man” model (M12); arm and leg models; Somso model (M11)] Spinal Nerves Cervical Plexus Phrenic nerve Brachial Plexus Function (Innervation) Head, Neck and Diaphragm Diaphragm, respiration Arm muscles and skin Axillary Nerve Musculocutaneous Nerve Radial Nerve Shoulder muscles and skin Anterior upper arm muscles/ lateral forearm skin Posterior/lateral upper arm and forearm muscles and skin Anterior forearm muscles and skin Medial forearm muscles and skin Innervates lower limb Median Nerve Ulnar Nerve Lumbar Plexus Femoral Obturator (missing on M12) Sacral Plexus Sciatic Nerve Tibial Common Fibular Location Cervical Cervical Plexus Cervical Enlargement Brachial Plexus Brachial Plexus Brachial Plexus Brachial Plexus Brachial Plexus Lumbar Enlargement Lower abdomen, anterior and medial Lumbar Plexus thigh muscles and skin Hip and medial thigh muscles and Lumbar Plexus skin Lower Limb Lower trunk, posterior thigh muscles Sacral Plexus and skin Branch of sciatic, posterior lower leg Sacral Plexus and foot muscles and skin Branch of sciatic, anterior and lateral Sacral Plexus lower leg and foot muscles and skin 3 Reflexes Note: the terminology and activities concerning reflexes are based on both the current lab manual by Wood (2013) as wells the former lab manual by Marieb and Mitchell (2009). Terms and activities not found in the current lab manual are in red and are optional. 1. Reflex Terminology: a. List the components of a reflex arc; identify each component on a diagram or model, and indicate the function of each. b. Distinguish monosynaptic and polysynaptic reflexes. c. Distinguish between an innate (inborn, basic) reflex from an acquired (learned) reflex d. Define the following terms with respect to reflexes: (see textbook and/or outside reference) 1) spinal reflex 2) inhibition 3) reciprocal inhibition 4) reinforcement 2. Reflexes Activities Perform the following reflexes with a partner. Describe the initiation and response of the following reflexes and indicate the spinal and/or cranial nerves involved in each. a. Somatic reflexes 1) Stretch reflexes (see current lab manual) a) patellar “knee jerk” b) Achilles “ankle jerk” c) biceps reflex d) triceps reflex 2) Cross-Extensor reflex (with the back of subject’s hand resting on a lab bench, prick subject’s index finger with a pencil while subject’s eyes are closed and observe response in both upper limbs) 3) Superficial cord reflexes a) abdominal (see current lab manual) b) plantar (Babinski’s) (stroke the plantar surface of bare foot with the handle of a reflex hammer moving from heel to ball of foot on lateral side, and then across to medial ball of foot. Observe toes spread apart and extend upward (positive response) or flex downward (negative response). 4) Cranial reflexes a) corneal (with cotton fibers of a sterile cotton swab, touch the surface of the eye and observe response) b) gag (touch a tongue depressor on the back of the throat and observe response) 4 b. Autonomic reflexes 3) pupillary (in a darkened room, shine a pen light in one eye) a) ipsillateral (look for pupil response in illuminated eye) b) consensual (contralateral) (look for pupil response in opposite eye while shielding opposite eye from light shown in other eye) 4) ciliospinal (gently stroke hair or pinch skin of subject’s neck, close to hairline on the left side of the back of neck and watch for pupil responses) c. Acquired (learned) reflexes Test the reaction time for catching a metric ruler between the thumb and index finger. Have a partner hold the ruler vertically 3 cm above the participant’s hand with the number 0 at the bottom. Compare the reaction time indicated by the number grasped when the ruler is caught with full concentration versus mental distraction (e.g. while subtracting 3 repeatedly from a large number: 100, 97, 94, 91, 88, etc). 5