Rev. 10-4-06 ACRIN-PA 4001 Protocol for positioning subjects and obtaining MR Images Procedure for positioning subjects for MRI examinations Purpose: Previous studies indicate variation in patient positioning are a substantial source of random error in MRI measurements of articular cartilage. It is important to take steps to reproducibly position the subject in the scanner for each study. INITIAL MRI EXAM 1. The target knee is selected. For normal subjects this is based on the flip of a coin (heads) right knee, (tails) left knee. For symptomatic subjects the target knee is selected based on the radiographic assessment of KL grade used during enrollment. 2. The subject is positioned supine feet first on the MRI table. The inferior pole of the patella should be placed at isocenter of the radiofrequency (RF) coil. 3. The heel of the target leg is placed in the ACRIN positioning device 4. The foam pad is placed behind the knee. The thickness of the pad should be adjusted to put the knee in approximately 5 degrees of flexion. If there are different pads used for this study then identify and record this pad on the subject encounter form for subsequent visits. 5. The positioning device is adjusted to achieve subject comfort, and the Velcro strap is secured around the foot and ankles. 6. Measure the distance between the edge of the coil and the edge of the footplate on the positioning device using a MRI compatible tape measure. This distance should be recorded on the subject encounter form for subsequent visits. 7. Position the subject in the magnet isocenter using the scanner laser positioning system MRI EXAMINATIONS 2, 3, AND 4 1. 2. 3. 4. 5. Identify the target knee and confirm with subject record Position the target knee in the coil with the inferior patellar pole at isocenter. Place the pad used in MRI visit 1 behind the knee Place the heel of the target leg in the ACRIN positioning device Adjust patient positioning to ensure the distance between the coil and positioning device matches that used in MRI visit 1. 6. Secure foot and ankle with Velcro straps 7. Position the subject in the magnet isocenter using the scanner laser positioning system Rev. 10-4-06 Procedure for planning coverage for MRI study TRIPLANE AND SAGITAL SCOUT IMAGES (FIGURE 1) The triplane scout should be centered at isocenter and provides 3 sections in the three standard orthogonal projections. The triplane scout is used to position the sagital scout that will be used in planning subsequent imaging planes. 1. Select axial image that includes the posterior margin of both femoral condyles 2. Draw line that passes through the posterior margin of the femoral condyle termed the posterior intercondylar line (PIL) (See Figure 1) 3. Position sagital scout perpendicular to the PIL PIL Figure 1: Axial scout image used to determine position of the posterior intercondylar line (PIL). The sagital scout images are positioned perpendicular to the PIL PLANNING CORONAL IMAGES (FIGURE 2) Coronal images obtained in this protocol consist of 3D WE-GRE MSME (T2 Mapping) T1rho weighted SSFP These sequences should provide the same anatomic coverage of the femoral tibial joint as outlined below: 1. Using the axial image from the tri-plane scout align the coronal plane parallel to the PIL 2. Select the sagital scout image that passes through the center of the medial femoral/tibial joint space 3. Adjust position of the coronal images to ensure coverage from the anterior to posterior edge of the tibial plateau Rev. 10-4-06 PIL Figure 2: Coronal images should be oriented parallel to the PIL. Using the sagital scout image obtained in the center of the medial femoral tibial coverage ensure coronal images extend from the anterior to posterior margin of the tibial plateau PLANNING AXIAL IMAGES (FIGURE 3) Axial images obtained in this protocol consist of 3D WE-GRE MSME (T2 Mapping) T1rho weighted SSFP These sequences should provide the same anatomic coverage of the patellofemoral joint as outlined below: 1. Select the scout image that passes through the center of the patella 2. Coverage on axial images should extend from the superior to inferior pole of the patella 3. Ensure phase encoding is in the left/right direction to prevent pulsation artifact from overlying the patellar cartilage. Figure 3: Coverage for axial images. Coverage should extend from the superior to inferior margin of the patella