Dr. Mei Chen
Professor of Research
Department of Dermatology
Other publications:
1. Flomenberg PR, Chen M, Horwitz MS: Characterization of MHC class I antigenbinding glycoprotein from adenovirus 35, a type associated with immunocompromised
hosts. J. Virol. 61:3665-3671, 1987.
2. Flomenberg PR, Chen M, Horwitz MS: The molecular epidemiology of adenovirus
type 35 isolated from immunocomprosed hosts. J. infec. Dis. 155:1127-1134, 1987.
3. Flomenberg PR, Chen M, Horwitz MS: Sequence and genetic organization of the
adenovirus type 35 early region 3. J. Virol. 62:4431-4437, 1988.
4. Chen M, Horwitz MS: Dissection of functional domains of adenovirus DNA
polymerase by linker insertion mutagenesis. Proc. Natl. Acad. Sci. USA 86:6116-6120,
1989.
5. Chen M, Mermod N, Horwitz MS: Protein-protein interaction between adenovirus
DNA polymerase and nuclear factor I mediate formation of the DNA replication
preinitiation complex. J. Biol. Chem. 265:18634-18642, 1990.
6. Chen M, Horwitz MS: Replication of adenovirus type 34 mutant DNA containing
tandem reiterations of inverted terminal repeat. Virol. 179:567-575, 1990.
7. Chen M, Horwitz MS: Protein-DNA interactions at the adenovirus origin of
replication: eukaryotic DNA polymerases. (Heidelberg: Springer-Verlag) 1991.
8. Chen M, Pan ZQ, Hurwitz J: Sequence and expression in E. coli of the 40-kDa subunit
of activator I (replication factor C) of Hela cells: Proc. Natl. Acad. Sci. USA 89:25162520, 1992.
9. Chen M, Pan ZQ, Hurwitz J: Studies of the cloned 37-kDa subunit of activator I
(replication factor C) of Hela cells. Proc. Natl. Acad. Sci. USA 89:5211-5215, 1992.
10. Pan ZQ, Chen M, Hurwitz J: The subunits of activator I (replication factor C) carry
out multiple functions essential for proliferating-cell nuclear antigen-dependent DNA
synthesis. PNAS USA. 90:6-10, 1993.
11. O'Donnell M, Onrust R, Dean FB, Chen M, Hurwitz J: Homology in accessory
proteins of replication polymerases E. coli to human. Nuclei Acid Res. 21:1-3, 1993.
12. Ozawa K, Dean B, Chen M, Lee SH, Shiratori A, Murakami Y, Sakakura T, Hurwitz
J, Eki T; Mapping of the 70kDa, 34kDa, and 11kDa subunit gene of the human
multimeric single-stranded DNA binding protein (hSSB/PRA) to chromosome bands
17p13, 1p35-p36, and 7p21-22. Cell Stru. and Fun. 18:221-230, 1993.
13. Chen M, Achkar C, Gudas LJ: Enzymatic conversion of retinaldehyde to retinoic acid
by cloned murine cytosolic and mitochondrial aldehyde dehydrogenases. Mol.
Pharmacology 46:88-96, 1994.
14. Okumura K, Nogami M, Taguchi H, Dean FB, Chen M, Pan ZQ, Hurwitz J, Shiratori
A, Murmakami Y: Assignment of the 36.5-Kda (RFC5), 38-kDa (RFC3) and 40-kDa
(RFC2) subunit genes of human replication factor C to human chromosome bands
12q24, 2-q24.3, 3q27, 13q12, 3-q13 and 7q 11.23 Genomics 25:274-278, 1995.
15. Chen M, Quintans, J, Weichselbaum RR: Suppression of Bcl-2 mRNA production
may mediate apoptosis after ionization radiation, TNF, and ceramide. Cancer Res.
55:991-994, 1995.
16. Xu JH, Kim S, Chen M, Wagner A, Hay N, Li W. Growth arrest by IFN-r: blocking
CSF-1-and phorbol ester-stimulated MAP kinase activation in the abscence of ISGFactivation. Blood 86:2774-2788, 1995.
17. Chan LS, Chen M, Woodley DT: Epidermolysis bullosa acquisita in the elderly:
clinical manifestation, diagnosis, and therapy. J. Geriatr Dermatol. 4:47-52, 1996.
18. Chen M, Petersen PJ, Hai-Li L, Cai X-Y, O’Toole EA, Woodley, DT: Ultraviolet A
irradiation up-regulates type VII collagen expression in human dermal fibroblasts. J.
Invest. Dermatol. 108:125-128, 1997.
19. Chen M, Goyal S, Cai X-Y, O'Toole EA, Woodley DT: Modulation of type VII
collagen (anchoring fibrils) expression by retinoids in human skin cells. Biochim. &
Biophys. Acta. 1351:333-340, 1997.
20. Chen M, Marinkovich MP, Veis A, O’Toole EA, Rao CN, Cai X-Y, Woodley DT:
Interactions of the amino-terminus noncollagenous domain (NC1) of type VII
collagen with extracellular matrix components: A potential role in epidermal-dermal
adherence in human skin. J. Biol. Chem. 272:14516-14522, 1997.
21. Chen M, Chan LS, Cai X-Y. O’Toole EA, Sample JC, Woodley DT: Development of
an ELISA for rapid detection of anti-type VII collagen autoantibody in EBA. J. Invest.
Dermatol. 108:68-72, 1997.
22. Chan LS, Majmudar AA. Tran HH, Schaumburg-Level G. Chen M, Woodley DT,
Marinkovich MP: Laminin –6 is a lamina densa autoantigen in oral mucosal
pemphigoid. J. Invest. Dermatol. 97:848-853, 1997.
23. She H-Y, Nishimura R, Chen M, Rockow S, Margolis B, Skolink E, Li W: Grb2 links
receptor tyrosine kinase to Wiskott-A; drich syndrome protein (WASP). Mol. Biol.
Cell 8:1709-1721, 1997.
24. Olivry T, Fine J-T, Dunston SM, Chasse D, Pascal TA, Monteiro-Riviere NA, Chen
M, Woodley DT: Canine epidermolysis bullosa acquisita: circulating autoantibodies
target the aminoterminal noncollagenous (NC1) domain of collagen VII in anchoring
fibrils. Vet. Dermatol. 9:19-31, 1998.
25. Chan LS, Vandelugt CJ, Hashimoto T, Nishikawa T, Zones JJ, Black MM,
Wojnarowask F, Stevens SR, Chen M, Fairley JA, Miller SD, Gordon KB: Epitope
spreading: lessons from autoimmune skin disease. J. Invest. Deramtol. 110:103-109,
1998.
26. Xu L, Chen M, Peng J, O’Toole EA, Woodley, DT, Chan, LS: Molecular cloning and
characterization of a cDNA encoding canine type VII collagen non-collagenous (NC1)
domain, the target antigen of autoimmune disease epidermolysis bullosa acquisita
(EBA). Biochim & Biophys Acta 1408:25-34, 1998.
27. Chen M, Marinkovich MP, Jones JJ, O’Toole EA, Woodley DT: NC1 domain of type
VII collagen binds to the beta 3 chain of laminin 5 via a unique subdomain within the
fibronectin-like repeats. J. Invest. Dermatol. 112:177-183, 1999.
28. Chan LS, Lapiere JC, Chen M, Mancini A, Paller A, Woodley DT: Bullous systemic
lupus erythematosus with IgG and IgA autoantibodies targeting multiple skin
basement membrane zone components. Arch. Dermatol. 135:569-573, 1999.
29. Chen M, O’Toole EA, Li YY, Woodley DT: 21 integrin mediates dermal
fibroblast attachment to type VII collagen via a 158 amino acid segment of the NC1
domain. Exp. Cell Res. 249:231-239, 1999.
30. Xu L, O’Toole EA, Olivry T, Hernandez C, Pen J, Chen M, Chan LS: Molecular
clonning of a canine bullous pemphigoid antigen 2 cDNA and immunomapping of
NC16A domain by canine bullous pemphigoid autoantibodies. Biochim & Biophys
Acta 1500:97-107, 1999.
31. Olivry T, Savary KC, Murphy KM, Dunston SM, Chen M: Bullous system lupus
erythematosus (type I) in a dog. Vet. Rec. 145:165-169, 1999.
32. Chen M, O’Toole EA, Muellenhoff M, Medina E, Woodley DT: Development and
characterization of a recombinant truncated type VII collagen ‘minigene’: implication
for gene therapy of dystrophic epidermolysis bullosa. J. Biol. Chem. 275:2442924435, 2000.
33. Gandhi K, Chen M, Aasi S, Lapiere JC, Woodley DT, Chen LS: Autoantibodies to
type VII collagen have heterogeneous subclass and light chain composition and their
complement activating capacity do not correlate with the inflammatory clinical
phenotype. J. Clin. Immuno. 20: 416-423, 2000.
34. Chen M, Keene DR, Costa FK, Tahk SH, Woodley DT: The carboxyl terminus of
type VII collagen mediates antiparallel dimer formation and constitutes a new
antigenic epitope for epidermolysis bullosa acquisita autoantibodies. J. Biol. Chem.
276:21649-21655, 2001.
35. Li W, Nadelman C, Henry G, Fan JF, Muellenhoff M, Medina E, Gratch NS, Chen
M, Han JH, Woodley DT: The p38MAPK/SAPK pathway is required for human
keratinocyte migration on dermal collagen. J. Invest. Dermatol. 117:1601-1611, 2001.
36. Hallel-Halevy D, Nadelman C, Chen M, Woodley DT: Epidermolysis bullosa
acquisita: update and review. Clin. Dermatol. 19:712-718, 2001.
37. Chen M, Costa FK, Lindvay CR, Han YP, Woodley DT: The recombinant expression
of full-length type VII collagen and characterization of molecular mechanisms
underlying dystrophic epidermolysis bullosa. J. Biol. Chem. 277:2118-21124, 2002.
38. Chen M, O’Toole EA, Sanghavi J, Mahmud N, Kelleher D, Woodley DT: The EBA
antigen (type VII collagen) is present in human colon and patients with Crohn’s
disease have autoantibodies to type VII collagen. J. Invest. Dermatol. 118:1059-1064,
2002.
39. Li W, Nadelman C, Gratch NS, Li WQ, Chen M, Kasahara N, Woodley DT: An
important role for protein kinase C- in human keratinocyte migration on dermal
collagen. Exp. Cell Res. 15:219-228, 2002.
40. Delbaldo C, Chen M, Friedli A, Prins C, Saurat JH, Woodley DT, Borradori L: Druginduced epidermolysis bullosa acquisita with antibodies to type VII collagen. J. Am.
Acad. Dermatol. 46:S161-164, 2002.
41. Chen M, Keene DR, Chan LS, Hoeffler WK, Finlay D, Kasahara N, Woodley DT:
Restoration of type VII collagen expression and function in dystrophic epidermolysis
bullosa. Nat. Genet. 32:670-675, 2002.
43. Jean-Baptiste S, O'Toole EA, Chen M, Guitart J, Paller A, Chan LS: Expression of
eotaxin, an eosinophil-selective chemokine, parallels eosinophil accumulation in the
vesiculobullous stage of incontinentia pigmenti. Clin. Exp. Immunol. 127:470-478,
2002.
44. Schmidt E, Chen M, Kuhn C, Weber L, Brocker EB, Zillikens D: Childhood
epidermolysis bullosa acquisita: a novel variant with reactivity to all three structural
domains of type VII collagen. Br. J. Dermatol. 147:592-597, 2002.
44. Chen M, Li W, Fan JH, Kasahara N, and Woodley DT: Lentiviral vectors mediate
highly efficient and sustained gene transfer in primary human skin cells. Clin. Exp.
Dermatol. 28:193-199, 2003.
45. Woodley DT, Krueger GG, Fairley JA, Atha T, Huang Y, Chan L, Keene DR, and
Chen M: Normal and gene-corrected dystrophic epidermolysis bullosa fibroblasts
alone can produce type VII collagen at the basement membrane zone. J. Invest.
Dermatol. 121: 1021-1028, 2003.
46. Li W, Fan J, Chen M, Guan S, Sawcer D, Bokoch GM, Woodley DT: Mechanism of
human dermal fibroblast migration driven by type I collagen and PDGF-BB. Mol.
Biol. Cell 15:294-309, 2004.
47. Woodley DT, Atha T, Huang Y, Lipman K, Keene DR, Li W, Chen M: Injection of
recombinant human type VII collagen restores collagen function in dystrophic
epidermolysis bullosa. Nat. Medicine 10:693-695, 2004
48. Li W, Henry G, Fan JF, Sawcer D, Chen M, Pang K, Woodley DT: The initiation,
augmentation and directionality signals for human keratinocyte migration. J. Invest.
Dermatol. 123:622-633, 2004.
49. Woodley DT, Atha T, Huang Y, Ramin R, Kasahara N, Keene DR, Chen M.
Intradermal injection of lentiviral vectors corrects regenerated human dystrophic
epidermolysis bullosa skin tissue in vivo. Mol. Ther. 11:318-326, 2004.
50. Woodley DT and Chen M. Epidermolysis bullosa: Then and now. J. Am. Acad.
Dermatol. 51:55-57, 2004.
51. Fairley JA, Woodley DT, Chen M, Giudice GJ, Lin MS: A patients with both bullous
pemphigoid and epidermolysis bullosa acquisita: A example of intermolecular epitope
spreading. J. Am. Acad. Dermatol. 51:117-121, 2004.
52. Li W, Fan J, Chen M, Woodley DT: Mechanisms of human skin cell motility. Histol.
Histopathol. 19:1311-1324, 2004.
53. Woodley DT, Chang C, Saddat P, Ram R, Li Z and Chen M: Evidence that anti-type
VII collagen antibodies are pathogenic and responsible for the clinical, histological
and immunological features of epidermolysis bullosa acquisita, J. Invest. Dermatol.
124:958-564, 2005
54. Li W, Dasgeb B, Phillips T, Li Y, Chen M, Garner W, Woodley DT: Wound-healing
perspectives. Dermatol. Clin. 23:181-192, 2005.
55. Fan JF, Guan X, Cheng CF, Cho M, Chen M, Woodley DT, Li W. Protein
kinase C clustering at the leading edge and mediating PDGF-enhanced, but
not collagen-initiated, dermal fibroblast migration. J. Invest. Dermatol.
126:1233-1243, 2006
56. Bandyopadhyay B, Fan J, Guan S, Li Y, Chen M, Woodley DT, Li W. A "traffic
control" role for TGFbeta3: orchestrating dermal and epidermal cell motility during
wound healing. J. Cell Biol. 172:1093-1105, 2006
57. Li Y, Chen M, Li W, Woodley DT: Transforming growth factor-alpha elevated in
human serum is the major pro-motility factor for human keratinocytes. J. Invest.
Dermatol. 2006, in press
58. Woodley DT, Ram R, Doostan A, Bandyopadhyay P, Huang Y, Keene DR and Chen
M: Induction of epidermolysis bullosa acquisita in mice by passive transfer of
autoantibodies from patients. J. Invest. Dermatol. 126:1323-1330, 2006
59. Chen M, Woodley DT. Fibroblasts as target cells for DEB gene therapy. J. Invest.
Dermatol. 126:708-710, 2006