Playing cards for Memory and Total recall
Pages 2 to 4: keyword-cards
Pages 5 to 7: keyword-cards with corresponding explanation-cards
Restriction
Restriction
fragment
endonuclease
IsoschizoGel
mer
electrophoresis
Growth
rate
Double
digestion
Ethidium
bromide
Origin of
replication
Star
activity
Slot
Electrophoretic
mobility
Selectable
marker
pBR 322
MCS
Polylinker
Complex
media
-Galactosidase
High copy
plasmid
Low copy
plasmid
Defined
media
DNA
ligases
Competent Escherichia
cells
coli
Ligation
OD
pUC 19
Electroporation
EcoR I
lac-Operon
Cloning
vector
Electrophoresis
Antibiotic
resistance
Restriction
map
Blunt end
enzymes
Endonucleases
DNA
band
Restriction
enzyme
Recognition
site
Ampicillin
Plasmid
Base
pair
Sticky end
enzymes
Palindrome
Target
sequence
Compatible
ends
Electropho- Restriction
resis marker
buffer
Salt
competent
lacZ gene
ORI
Heat shock
Bromophenol blue
Xylenxyanol
Type I
enzyme
Hybridisation
Probe
Label
Denaturation
Southern
Blot
Target
sequence
Expression
vector
Promoter
Terminator
Southern
Blot
Western
Blot
Agarose
PCR
Primer
Annealing
Synthesis
Cycle
RBS
Cassette
DNA modifying enzymes that
recognise specific motifs of
double stranded DNA and
cleave it into fragments of
different lengths
sequence motif with an axis
of rotational symmetry, the
sequence in 5`3`-direction
is equal on the both strands
Endonucleases
Palindrome
vehicle of genetic engineering
shuttle that allows reintroducing
of foreign DNA into a host
organism
small rings of double stranded
extrachromosomal and
independently reproduced DNA,
natural types are important for
antibiotic resistance and sexual
reproduction of bacteria
type II restriction enzyme:
recognises a specific
palindrome sequence
motif (GAATTC)
Vector
Plasmids
EcoRI
bacterial plasmids with features
for reintroducing of small foreign
DNA-fragments
into
host
organisms with the purpose of
producing significant amounts of
recombinant DNA
Cloning vector
diagram showing sites of
cleavage of a DNA molecule,
for example of a plasmid
vector
Restriction map
Cleavage of DNA with this type
of endonucleases creates a short
complementary single stranded
overhang in each cleaved end
Sticky end
enzymes
Endonucleases that form no 5’
or 3’ overhangs after cleavage
Blunt end
enzymes
Bacterial cells which are able
to incorporate foreign DNA
Competent cells
Bacterial expression unit of
genes for lactose utilisation
lac-Operon
Method that separates DNA
fragments in an electric field
as a function of their lengths
Agarose gel
electrophoresis
Restriction enzymes that
recognise the same target
sequence
Isoschizomer
Staged recognition sites of
different restriction enzymes, a
feature of vectors for variable
insertion of foreign DNA
Polylinker
Stain that intercalates into
double-stranded DNA. Under
UV-light the complex of DNA
and the stain fluoresces pink
Ethidium bromide
(EtBr)