Cyanobacterial Biodiversity
T.Bhuvaneshwari,
Research Scholar,
NFMC.
Cyanobacteria – An Introduction
• Cyanobacteria - morphologically distinct group of Oxygenic
Photosynthetic organisms
• Gram negative prokaryote - low state of cell organization
• The cell lacks well defined nucleus and the DNA floats in the
protoplast
• Cyanobacteria exist in all known photic habitats and can thrive in
extreme levels of humidity, light, salinity, temperature, availability of
oxygen or cabon di oxide and solar radiation
Cyanobacterial Taxonomy
Botanical Approach
Morphological and cytological (Bornet and Flahaut,
Gomont, Geitler and desikachary)
Bacteriological Approach
Polyphasic Approach- physiological,Cytological, and
biochemical (Stanier, waterberry and Ripka)
Molecular Approach
Polyphasic Approach - phenotypic and Genotypic
characters (Anagnostidis and Komarek )
Identification at the Genetic level
Project Objectives
• Upgradation and maintanence of marine cyanobacteria in the Repository
• Axenization of the Marine cyanobacteria
• Identification of the strains by adapting morphological and Molecular
Methods
• Revalidation of the strains for their identify, lipid and fatty acid content
• Supply of cultures to the needy researchers
• Bioprospecting of marine cyanobacteria for Biofuel production
 Screening of cultures for lipid and fatty acid profile
 Optimization of physico chemical parameters for high lipid production.
Aim of MY Study
•
•
•
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To maintain the Repository of Marine cyanobacteria
To upgradate the strength of the Repository by isolating new strains
To identify the strains Morphologically
To Characterise the isolated cyanobacteria using Molecular approach such as
 16SRNA sequencing
 ITS (internal transcribed spacer region)
 cpc region
Repository Maintanence
Marine cyanobacterial strains of the Repository
were maintained in both Solid and Liquid culture.
Liquid cultures were subcultured regularly at the interval of 15
days and incubated at 25±2ºC under continuous low light
illumination.
For long Preservation, the cultures were also maintained in
Agar Slants
Isolation of marine Cyanobacterium
Samples are taken from different environments covering Rocky shores ,
Sandy shores, Saltpans, Backwater and Estuarine areas of Rameshwaram,
Krusadai, Cuddalore, Vizhingam, Jalgoan, Tuticorn and Andaman
They are processed immediately and pure cyanobacteria were isolated using
Standard Microbiological Protocols
isolated strains so far
Isolate No
Organism
Sample Area
Sample No
1
Chroococcus species
Vizhingam
03
2
Gleocapsa crepidinum
Gujarat
12
3
Myxosarcina burmensis
Vizhingam
01
4
Aphanothece saxicola
Gujarat
08
5
Aphanothece saxicola
Gujarat
09
6
Oscillatoria probosideae
Tuticorn
153
7
Oscillatoria species
Tuticorn
34
8
Oscillatoria species
Cuddalore
16
9
Phormidium species
Rameshwaram
05
10
Phormidium species
Krusadai
01
11
Phormidium tenue
Krusadai
11
12
Nostoc calcicola
Gujarat
06
Further 8 pure isolated strains yet to be identified
Chroococcus species
Gleocapsa crepidinum
Myxosarcina burmensis
Aphanothece saxicola
Aphanothece saxicola
Oscillatoria probosideae
Oscillatoria species
Oscillatoria species
Oscillatoria species
Phormidium tenue
Phormidium species
Phormidium species
Nostoc calcicola
Molecular Approaches
Molecular work carried out so far
DNA were isolated for ten organism by Xanthogenate
method
Among them, Pcr was carried out for four samples using
the Primer 16s-1F and 16s 740 R for amplifying the regions
of 16s rDNA
It was then Send for sequencing the results obtained were
not satisfactory as the chromatogram was not good and
also the sequence matches with certain bacterial 16s rDNA
sequence when performed blast analysis with the available
nucleotide sequence of NCBI.
Other markers to be studied
Mcy (Microcystin synthesizing gene)
Gyr B (Gyrase B)
rpo C (RNA Polymerase B)
rpo D (RNA Polymerase D)
• Thank
ThaNK you