Archie Clements
University of Queensland
School of Population Health
• Introduction to geostatistics
– Assumptions
– Variogram components
– Variogram models
– Kriging
– Assumptions
• Model-based geostatistics
– Principles
– Building the model
– Prediction
– Validation
• Applications: parasitic disease control in Africa
Z
Y
X
• First-order variation:
– Trend
– Large-scale variation
– Can be due to large-scale environmental drivers (e.g. temperature for vector-borne diseases)
• Second-order variation:
– Localised variation: clustering
– Modelled using geostatistics
• Observations close in space are more similar than observations far apart
• The variance of pairs of observations that are close together (small h ) tends to be smaller than the variance of pairs far apart
(large h )
• Basis of the semivariogram
– Spatial decomposition of the sample variance
Semivariance is half the average squared difference of values observed at locations separated by a given distance (and direction)
Function of distance (and direction); distance in bins, direction in sectors of compass – “azimuth”
Modelling spatial correlation: semivariogram
Partial
Sill
Nugget
Lag ( h )
Sill
• Random variation (white noise); nonspatial measurement error
• Microvariation (spatial variation at a scale smaller than the smallest bin)
• If no spatial correlation:
– Nugget = sill (flat semivariogram)
Semivariogram: decisions to be made
• How many/what sized bins?
– Depends on density of data points
– For regular-spaced (grid-sampled) data bin size = size of cells in the grid
– For irregular sampling – modify according to range of spatial correlation (big range, big bins; small range, small bins)
• What maximum lag( h ) to use?
– Should be estimated up to half the length of the shortest side of study area
• Which parametric model to use?
– Visual fit
– Statistical fit
Omnidirectional semivariograms
• Spatial dependence is different in different directions
– Semivariogram calculated in one direction is different from semivariogram calculated in another direction
– Should check for anisotropy and, if present, accommodate it in interpolation
– Range or sill (or both) can differ
Schistosoma mansoni , Uganda: directional semivariograms
Direction
Range
(km)
Sill Nugget
Omnidirectional
0˚
45˚
90˚
135˚
43.4
7E-2
39.4
43.6
1E-1
7E-2
35.8
39.5
8E-2
1E-1
4E-2
-3E-3
2E-2
3E-2
2E-2
Schistosoma haematobium, Northwestern
Tanzania
Direction
Omnidirectional
0˚
Range
(km)
36.0
Sill
5E-2
260.1
2E-2
Nugget
0
3E-2
45˚
90˚
135˚
163.9
56.2
97.7
6E-3
5E-2
3E-2
3E-2
0
7E-3
Schistosoma haematobium ,
Northwestern Tanzania
• Data should be de-trended
– Polynomials (regression on XY coordinates)
– Generalised linear models (regression on covariates)
– Generalised additive models (can over-fit)
– If directional variograms are calculated & range in one direction is >3 X range in perpendicular, sign of trend
• If skewed, consider transformation (e.g. log transformation, normal score transformation)
– Otherwise, extreme values overly influence interpolated map
– Have to back-transform interpolated values
– Called “disjunctive Kriging”
• Spatial correlation structure cannot be generalised to the whole study area
• Why does it occur?
– Different factors may operate in different parts of the study area
– Different ecological zones with different disease epidemiology
• Need to estimate the spatial correlation structure separately in each homogeneous zone
Z(s i ) is the measured value at the i th location
λ i is the weight attributed to the measured value at the ith location
(calculated using semivariogram)
S o is the prediction location
For formulae on how the weights are estimated using the variogram: http://en.wikipedia.org/wiki/Kriging
Prediction standard error/variance gives an indication of precision of the prediction
• Geostatistics involves 3 steps:
– Exploratory data analysis
– Definition of a variogram
– Using the variogram for interpolation (Kriging)
• Technique applicable for:
– Point-referenced data
– Spatially continuous processes:
• Disease risk
• Rainfall, elevation, temperature, other climate variables
• Wildlife, vegetation, geology (mineral deposits)
Seminal paper:
Diggle, Tawn and Moyeed (1998). Model-based geostatistics. Appl.
Stat. 47:3;299-350
Observed a need for addressing non-Gaussian observational error
Idea is “to embed linear Kriging methodology within a more general distributional framework”
Generalised linear models with an unobserved Gaussian process in the linear predictor
Implemented in a Bayesian framework
Advantages of the Bayesian approach
• Natural framework for incorporation of parameter uncertainty into spatial prediction
– Can build uncertainty into parameters using priors
• Non-informative
• Informative (based on exploratory analysis, additional sources of information)
• Convenient for modelling hierarchical data structures
• Can predict at specified validation locations (with observed outcomes for comparison)
• Can predict at non-sampled locations, e.g. a prediction grid
• Might be interested in
– outcome
– spatial random effect
– Standard error of predicted outcome
• Jack-knifing; sampling with replacement
– Remove one observation, do prediction at that location and store predicted value
– Repeat for all observations
– Compare predicted to observed using statistical measures of fit
(RMSE) and discriminatory performance (AUC)
– Not feasible with MBG other than with v. small datasets
• Cross-validation; sampling without replacement
– Set aside a subset for validation (ideally 50%)
– Use remaining data to “train” model
– Compare predicted and observed for the validation subset using statistical measures
– Can then recombine the validation and training subsets for final model build
• External validation: using other prospective or retrospective dataset
Model-based geostatistics involves:
1.
Visual and exploratory data analysis
2.
Variography (to determine if there is secondorder spatial variation)
3.
Variable selection (for deterministic component)
4.
Building model (e.g. in WinBUGS)
5.
Model selection (e.g. using DIC)
6.
Prediction and validation
Schistosomiasis
779 million people at risk
207 million infected
Most in Africa
Significant illness and mortality Two main forms in Africa:
Urinary schistosomiasis caused by Schistosoma haematobium
Intestinal schistosomiasis caused by S. mansoni
Adult worm in human bladder wall
Cercariae released
Sporocysts in snail
Eggs in urine
Miracidia
S. haematobium:
Microscopic examination of urine slides: Presence of eggs and egg counts
Macrohaematuria (visible blood)
Microhaematuria (invisible blood) – tested using chemical reagent strips
Blood in urine questionnaire
S. mansoni and soiltransmitted helminths:
microscopic examination of stool samples
• School-aged children have highest prevalence
(proportion infected) and intensity (severity) of infection
• Education system is convenient for control; central location to access target population
• World Health
Organisation guidelines: treat communities biannually where prevalence in school-age children is >10% and annually where prevalence >50%
• No surveillance
• Need to do surveys
Field survey: northwest
Tanzania
Lake Victoria
153 schools surveyed
60 children per school
What about non-sampled locations? Need to predict
(interpolate) values
Y i
~ binomial ( n i
, p i
) logit ( p i
)
i
i
rain i
LST 1 i
LST 2 i
i
i
f ( d ij
;
)
exp
(
d ij
)
S. haematobium model results к
φ
Variable
Intercept
Coefficient
1.9 (-2.3 - 10.3)
Odds Ratio
–
LST >35-39C 0.4 (-0.3 - 1.1) 1.5 (0.8 - 2.9)
LST >39C 0.3 (-1.5 - 2.2) 1.4 (0.2 - 8.6)
Rainfall >1050mm -1.1 (-3.4 - 1.1) 0.3 (3.3 x 10 -2 - 3.1)
0.9 (0.6 - 1.3)
0.2 (0.1 - 1.0)
–
–
Clements et al. TMIH 2006
Lower bound: 95% PI
Upper bound: 95% PI
Co-ordinated surveys in 3 contiguous countries
•418 schools
•>26,000 children
Probability that prevalence is
>50%
Clements et al. EID 2008
Variable
Sex: Female
Age: 9–10 years
Age: 11–12 years
Age: 13–16 years
Distance to perennial water body
Land surface temperature
Land surface temperature 2
Rate of decay of spatial correlation
Variance of the spatial random effect (sill)
Mean (95% CI)
0.70 (0.65, 0.76)
1.16 (1.00, 1.33)
1.51 (1.31, 1.73)
1.79 (1.53, 2.06)
0.34 (0.21, 0.54)
0.80 (0.51, 1.21)
1.10 (0.85, 1.40)
2.03 (1.48, 2.74)
7.03 (5.36, 9.31)
SD
0.03
0.08
0.10
0.14
0.08
0.18
0.14
0.32
1.01
Other outcomes: co-infection
East Africa: Brooker and
Clements, Int. J. Parasitol., in press
S. mansoni mono-infection:
7.9%
Hookworm mono-infection:
40.5%
25
20
15
10
5
0
45
40
35
30
Co-infection: 8.1%
50
S. mansoni mono-infection
Hookworm mono-infection
Co-infection
0 10 20 30 40 50
% infected
60 70 80 90 100
Lake
Albert
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UGANDA
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Lake Victoria
KENYA
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TANZANIA
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Infection status
No infection
S. mansoni monoinfection
Hookworm monoinfection
Coinfection
Country borders
Large water bodies
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Slide 38
100 200
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N
300 Kilometers
,
Y ijk
~Multinomial( p ijk
,n ijk
), p ijk
k
ijk n ijk log (
ijk
)
k
ik
N
T
1 , k
Nk
x
Nijk
ik f ( d ij
;
)
exp
(
d ij
)
Variable
Intercept
OR: Elevation
OR: DPWB
OR: Rural vs urban
OR: Ext. rural vs urban
OR: LST
OR: Female
OR: Age (9-10 years)
OR: Age (11-13 years)
OR: Age (≥14 years)
Phi (rate of decay)
Sill
S. mansoni monoinfection posterior mean
(95% posterior CI)
-3.8 (-4.7 - -2.9)
0.35 (0.22 - 0.58)
0.23 (0.10 - 0.45)
0.43 (0.21 - 0.79)
0.62 (0.23 - 1.44)
0.88 (0.62 - 1.25)
0.86 (0.76 - 0.96)
1.67 (1.37 - 2.06)
2.44 (2.06 - 2.89)
2.87 (2.19 - 3.71)
3.52 (1.73 - 7.21)
6.39 (3.52 - 11.78)
Hookworm monoinfection posterior mean
(95% posterior CI)
-0.6 (-1.1 - -0.3)
0.77 (0.65 - 0.89)
0.94 (0.76 - 1.15)
0.98 (0.68 - 1.37)
1.16 (0.82 - 1.81)
0.60 (0.50 - 0.72)
0.91 (0.86 - 0.97)
1.17 (1.04 - 1.30)
1.55 (1.39 - 1.71)
1.88 (1.63 - 2.14)
4.98 (3.38 - 7.33)
1.31 (0.98 - 1.76)
S. mansoni/hookworm co-infection posterior mean
(95% posterior CI)
-4.4 (-5.0 - -3.7)
0.30 (0.20 - 0.47)
0.30 (0.18 - 0.58)
0.61 (0.36 - 1.02)
0.75 (0.31 - 1.62)
0.57 (0.31 - 0.87)
0.70 (0.63 - 0.77)
1.82 (1.52 - 2.21)
2.99 (2.55 - 3.52)
3.83 (3.01 - 4.86)
3.76 (2.10 - 7.36)
6.34 (3.98 - 9.95)
S. mansoni monoinfection
Hookworm monoinfection
S. mansoni -
Hookworm coinfection
Prevalence is used (currently) for disease control planning
Intensity of infection (eggs/ml urine or /g faeces) is more indicative of:
Morbidity (anaemia, urine tract, hepatic pathology)
Transmission
Y ij
~ negbin ( mu ij
) log ( mu ij
)
girl ij
j
j
dist j
elev j
j
j
f ( d jk
;
)
exp
(
d jk
)
Clements et al. Parasitol 2006
Variable
Intercept
Female
Elevation (m)
DPWB (dec deg)
Sill
Range
Overdispersion
Posterior Mean (95% CI)
10.06 (5.77 - 13.22)
-0.41 (-0.72 - -0.11)
-0.007 (-0.01 - -0.004)
-5.36 (-7.51 - -3.30)
23.96 (19.06 - 32.07)
0.134 (0.09 - 0.20)
0.06 (0.058 - 0.062)
Slide 44
• In disease control we need evidence-based framework for deciding on where to allocate limited control resources
• Maps are useful tools for highlighting sub-national variation; targeting interventions; advocacy (national and local); integrated control programmes; estimating heterogeneities in disease burden
• Model-based geostatistics enables rich inference from spatial data; uncertainty