CELL MEMBRANES and TRANSPORT AS Biology. Foundation. Cell membranes and Transport 1 The Cell AS Biology. Foundation. Cell membranes and Transport 2 Cell membrane All living things are surrounded by a membrane. A cell membrane is also known as plasma membrane. Controls exchange of materials such as nutrients and waste between cells and their environment. Has other important functions for example to enable cells to receive hormones. To understand the function of anything in biology, you must study the structure first! AS Biology. Foundation. Cell membranes and Transport 3 Cell Membranes from Opposing Neurons (TEM x436,740). Nerve cell Cell membrane { Gap between cells } cell membrane 7nm wide Nerve cell AS Biology. Foundation. Cell membranes and Transport 4 Cell membranes are made of PHOSPHOLIPIDs HYDROPHILIC heads (water liking) -Attracted to the water called POLAR HYDROPHOBIC tails (water fearing) -Not attracted to the water called NON-POLAR AS Biology. Foundation. Cell membranes and Transport A Phospholipid 5 A phospholipid HYDROPHILIC HEAD At the other end of the phospholipid is a phosphate group and several double bonded oxygens. The atoms at this end of the molecule are not shared equally. This end of the molecule has a charge and is attracted to water. It is POLAR HYDROPHOBIC TAILS The two long chains coming off of the bottom of this molecule are made up of carbon and hydrogen. Because both of these elements share their electrons evenly these chains have no charge. They are NON POLAR. Molecules with no charge are not attracted to water; as a result water molecules tend to push them out of the way as they are attracted to each other. This causes molecules with no charge not to dissolve in water. AS Biology. Foundation. Cell membranes and Transport 3D model of a Phospholipid 6 A Phospholipid Bilayer Phospholipids can form: BILAYERS -2 layers of phospholipids with hydrophobic tails protected inside by the hydrophilic heads. The PHOSPHOLIPID BILAYER is the basic structure of membranes. AS Biology. Foundation. Cell membranes and Transport 7 Fluid mosaic model Cell membranes also contain proteins within the phospholipid bilayer. This ‘model’ for the structure of the membrane is called the: FLUID MOSAIC MODEL FLUID- because individual phospholipids and proteins can move around freely within the layer, like it’s a liquid. MOSAIC- because of the pattern produced by the scattered protein molecules when the membrane is viewed from above. AS Biology. Foundation. Cell membranes and Transport 8 Cell Membranes from Opposing Neurons (TEM x436,740). } Phospholipid Bilayer 7nm wide AS Biology. Foundation. Cell membranes and Transport 9 Movement of selected molecules across the cell membrane AS Biology. Foundation. Cell membranes and Transport 10 Methods of Transport Across Membranes 1. Diffusion 2. Osmosis 3. Facilitated Diffusion 4. Active Transport AS Biology. Foundation. Cell membranes and Transport 11 Methods of Transport Across Membranes Diffusion -passive transport - no energy expended 2. Osmosis - Passive transport of water across membrane 3. Facilitated Diffusion - Use of proteins to carry polar molecules or ions across 4. Active Transport- requires energy to transport molecules against a concentration gradient – energy is in the form of ATP 1. AS Biology. Foundation. Cell membranes and Transport 12 Diffusion Movement of molecules from an area of high concentration to an area of low concentration. Movement from one side of a membrane to another, unfacilitated AS Biology. Foundation. Cell membranes and Transport 13 Diffusion AS Biology. Foundation. Cell membranes and Transport 14 Solutions Solutions are made of solute and a solvent Solvent - the liquid into which the solute is poured and dissolved. We will use water as our solvent today. Solute - substance that is dissolved or put into the solvent. Salt and sucrose are solutes. AS Biology. Foundation. Cell membranes and Transport 15 Osmosis AS Biology. Foundation. Cell membranes and Transport 16 Tonicity is a relative term Hypotonic Solution - One solution has a lower concentration of solute than another. Hypertonic Solution - one solution has a higher concentration of solute than another. Isotonic Solution - both solutions have same concentrations of solute. AS Biology. Foundation. Cell membranes and Transport 17 Plant and Animal Cells put into various solutions AS Biology. Foundation. Cell membranes and Transport 18 Types of Transport AS Biology. Foundation. Cell membranes and Transport 19 Diffusion Lab We are using dialysis tubing as the cell membrane - It is selectively permeable The solute is either the eosin starch solution or the sugar solution What is the solvent? AS Biology. Foundation. Cell membranes and Transport 20 Membrane Permeability Solute is eosin-starch-chloride solution Solvent is Water Indicator for presence of starch is IKI Starch is made of amylose and amylopectin – amylopectin is insoluble Iodine is not very soluble in water, but with KI it forms a I3- (triiodine ion) which is soluble. I3- combines with the amylose and the starch molecule turns blue-black. AS Biology. Foundation. Cell membranes and Transport 21 Test for Starch AS Biology. Foundation. Cell Elmhurst College membranes andWebsite Transport 22 Test for Chloride ions Indicator for presence of chloride ions is silver nitrate, AgNO3 A white precipitate, AgCl, forms if chloride is present. AS Biology. Foundation. Cell membranes and Transport 23 Test for Sulfate ions Indicator for the presence of sulfate ions is Barium chloride, BaCl Barium sulfate, BaSO4 - forms a white precipitate BaCl + NaSO4 ----> BaSO4 + NaCl AS Biology. Foundation. Cell membranes and Transport 24 Living Cells Fern gametophytes are used to see result of putting live cells in solutions of varying tonicity AS Biology. Foundation. Cell membranes and Transport 25 Osmosis We will make an osmometer to see osmosis Sugar solution in a dialysis tube is used to simulate a cell membrane. Various concentrations of solute may be used around the room. AS Biology. Foundation. Cell membranes and Transport 26 Moving amoeba AS Biology. Foundation. Cell membranes and Transport 27