Crystal structure of Ice and Protein PHYS 571 Yeliz Celik Young Eun Choi Andrew DiLullo Outline Ice Physical Properties Types of Ice Hexagonal Ice Structure (Ice Ih) Hydrogen bonding and the hexagonal structure of ice The hexamer and the hexagonal crystal structure Protein Crystallization Examples of Protein structures Ice Physical Properties CHEMISTRY H2O , Hydrogen dioxide CRYSTALLOGRAPHYH Hexagonal (Ih) CRYSTAL GROWTH AND HABITS COLOR AND OTHER OPTICAL PROPERTIES Generally flat hexagonal crystals Ice also forms rounded and concentrically zoned spheres as hail stones. It can be stalactitic, and massive granular. Clear to white, Pale blue, Greenish blue, transparent to translucent HARDNESS 1.5 DENSITY 0.9167 g/cm3 BREAKABILITY Very brittle fracture producing small, conchoidal fragments Water Phase Diagram and Morphology Diagram http://www.lsbu.ac.uk/water/phase.html http://www.its.caltech.edu/~atomic/snowcrystals/primer/primer.htm Types of Ice - With both cooling and pressure different types exist : Ice II,III, V, VI, VII,VIII, IX, and X. The types are differentiated by their crystalline structure, ordering and density. Ice II (rhombohedral) Ice III (tetragonal) Ice VI (tetragonal) Ice VIII (tetragonal) Ice V (monoclinic) Ice X (cubic) - There are also two metastable phases of ice under pressure, both fully hydrogen disordered: IV and XII. Ices XI, XIII, and XIV are hydrogen-ordered forms of ices Ih, V, and XII respectively. Types of Ice - Everyday ice and snow has a hexagonal crystal structure (ice Ih). - Only a little less stable (metastable) than Ih is the cubic structure (Ic). Hexagonal Ice Structure (Ice Ih) c(a3) γ =120° a2 a1 a a ,c= •Classic snowflake from vapor-rich 1= formed 2 air between -10 to -22 ºC . LT-SEM image 7.35Å with inset4.51Å light a= © Eric Erbe / Beltsville Agricultural Research Center Prism face •Hexagonal ice plate formed from vapor deposition in air at 0 to -3 or -10 to -35 ºC. http://www.lsbu.ac.uk/water/ice1h.html LT-SEM image with inset light microscope image. Hydrogen Bonding in Ice Hydrogen bonding in general: shared bonding of H between two highly electronegative atoms such as F, O, or N Hydrogen bonding in water and ice: isolated two molecule (dimer) system Hydrogen bonding and the hexagonal structure of ice Other isolated clusters of ice molecules trimer, tetramer, pentamer, hexamer hexamer is the most energetically likely Isolated groups only somewhat useful in bulk study The hexamer and the hexagonal crystal structure of ice 2.8Å O-O distance Reason for decreased density of ice Expect 4 hydrogen bonds per molecule Measured 3.69 hydrogen bonds per molecule Protein Crystallization 1. 2. 3. Applications: Drug design and structural biology Bio-separation Controlled drug delivery Protein Crystallography 1. 2. 3. X-ray crystallography : Protein structures at the atomic level How proteins interact with other molecules How they undergo conformational changes Spruce Budworm Antifreeze Protein Insect antifreeze protein Molecular weight is ~9 kDa Protects from freezing at T below 0 degrees Protein Crystallography Pure protein (an insect antifreeze protein) Sample needs to be concentrated Dilute buffer Hanging drop vapor diffusion method Hanging Drop Vapor Diffusion Method http://www.bio.davidson.edu/COURSES/Molbio/MolStudents/spring2003/Kogoy/protein.html Crystals of Spruce budworm antifreeze proteins 0.21X 0.12X 0.02 mm After repeated seeding a nice crystal obtained Leinala et al.,2002: Grather,SP et al.,1999 Crystals of recombinant Spruce budworm antifreeze proteins Leinala et al.,2002 Result of x-ray crystallography is a three dimensional map that shows the distribution of electrons in the structure. http://www.jic.ac.uk/staff/david-lawson/xtallog/summary.htm Crystal structure of the protein: Single Anomalous Scattering, NMR Left-handed Beta helical structure 15 amino-acid loops Leinala, EK et al., 2002 How shape morphology changes when ice crystal is grown in a solution of the insect antifreeze protein References Petrenko,VF, et al.,Physics of Ice,1999, Oxford University Press, New York Grather,SP et al.,1999, Journal of Structural Biology,126,72-75. Leinala, EK, 2002, Structure,10,619-627. http://www.bio.davidson.edu/COURSES/Molbio/MolStudents/spring 2003/Kogoy/protein.html http://www.its.caltech.edu/~atomic/snowcrystals/primer/primer.htm http://www.lsbu.ac.uk/water/phase.html http://www.jic.ac.uk/staff/david-lawson/xtallog/summary.htm http://www.lsbu.ac.uk/water/phase.html http://www.its.caltech.edu/~atomic/snowcrystals/primer/primer.htm