OVERVIEW
R&D, Laboratories and Analysis Services
Gastien Godin
General Manager
Fredericton , NB
October 8th, 2014
Jacques Gagnon, PhD
Scientific Director
Fishery and Marine Coproducts Division
COASTAL ZONES RESEARCH INSTITUTE (CZRI)
• LAND & PEATLANDS MANAGEMENT- AGRICULTURE &
RENEWABLE RESOURCES- ADAPTATION TO CLIMATIC CHANGE
• AQUACULTURE
• FISHERY AND MARINE COPRODUCTS
• LABORATORIES AND ANALYSIS SERVICES
• CZRI Highlights – ValueAdded Food Sector
• Mobile Pilot Unit
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LAND & PEATLANDS MANAGEMENT- AGRICULTURE
& RENEWABLE RESOURCESADAPTATION TO CLIMATIC CHANGE
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Dr. Mathieu Quenum – Scientific Director
Email : mathieu.quenum@irzc.umcs.ca
Tel. : (506) 336-6600
Fields of Expertise and Strategic Research Concentration
Biomass & Bioenergy
Horticulture, Agriculture & Berries
Sustainable Production of Sphagnum & Added Value Products
Soil, Water & Environment
Sustainable Development in Coastal Areas
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AQUACULTURE
Dr. André Dumas– Scientific Director
Email : andré.dumas@irzc.umcs.ca
Tel. : (506) 336-6600
Fields of Expertise
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Nutrition of fish and crustaceans
Nutrition modelling
Arctic charr, salmon, oyster and
lobster production
Product development and
innovation management
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Research focus areas
Nutritional evaluation of commodity
and innovative ingredients, prebiotics,
probiotics, immunostimulants
Nutrigenomics
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FISHERY AND MARINE COPRODUCTS
Dr. Jacques Gagnon– Scientific Director
Email : jacques.gagnon@irzc.umcs.ca
Tel. : (506) 336-6600
Fields of Expertise
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Development of green processes
Lab scale spaces with hydrolysis, drying & centrifugation capacities
53’ pilot multifunctional unit for turnkey project on site
Product development and innovation management
Research focus areas
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Valorization and commercialisation
of marine coproducts
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Natural health products
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LABORATORIES AND ANALYSIS SERVICES
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Chemical/microbiological analyses
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Environmental, nutritional, etc.
Food safety
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ISO/IEC 17025 (CALA)
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R&D support
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Fatty acids & pigments
Amino acids (in development)
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Personnalized services
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On-site, technical, process design, etc.
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Regulatory support, nutritional labelling, shelf life
Coproducts recovery and characterization
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Mobile Pilot Unit
• Various tanks (2 x 1500 liters)
• 8 x 100 liters double walls for
temperature control
• Various pumps
• Filtration unit (sweco)
• Mixers
• Etc.
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53 feet reefer trailer
Processing room with temp. control
Walk-in cold room and freezer
600, 220, 110 volts connections
OPEN FOR BUSINESS
We look forward to new collaboration
opportunities!
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Highlights – Value-Added Food Sector
• CZRI co-hosted (with AFBPA) the Sixth Acadian Peninsula
Food Processing Conference
“Growing Our Industry: Making history with the new biotechnologies”
www.shippagan.ca
Acadian Peninsula Convention Centre,
Shippagan, NB May 21-23, 2014
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MAXIMIZING THE VALUE
OF MARINE COPRODUCTS:
APPLICATIONS IN NUTRISCIENCES
Jacques Gagnon, PhD and colls.
Fisheries Council of Canada 2014 Conference
October 8th
Valorization of effluents, residues
and marine coproducts
VALEUR.COM

VALEUR.COM = VALorisation des
Effluents d'Usine, des Résidus et des
COproduits Marins)
 Full title –AIF project:
DEVELOPMENT AND COMMERCIALIZATION OF
MARINE COPRODUCTS FOR USE IN ANIMAL AND
HUMAN NUTRITION AND ITS APPLICATION TO THE
PREVENTION OF OBESITY, DIABETES AND
NEURODEGENERESCENCE
 CZRI research team:
Nadia Tchoukanova
Sébastien Plante and Jacques Gagnon
BioAtlantech Survey (Biosciences)
 Geographical clustering
aquaculture and marine
 St. George/St. Andrews (7
Companies + 2 Research
Institutions)
 Acadian Peninsula (7
Companies + 1 Research
Institution)
Now BioNB, 2013
LIST OF COPRODUCTS
SEA, PROCESSING AND AQUAFEED: A
VALUE CREATION PATHWAY OF
SEAFOOD RAW MATERIALS
André Dumas, PhD & colls
The Fisheries Council of Canada 2014 conference
MT (millions)
Aquaculture in the World
100
80
60
40
20
0
2000
2010
2020
Source: FAO (2012)
Aquafeeds in the World
• 20 to 23 millions MT aquafeed/yr (2006)
• ~3.7 million MT/yr fish meal (FM) = $5.5 bln
• ~0,8 million MT/yr of fish oil (FO) = $1.0 bln
Source: Tacon & Metian (2008)
FM: source of beneficial nutrients
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Essential amino acids (AA)
Valuable AA: Taurine, hydroxyproline
Vitamins (e.g. B12)
Minerals (e.g. available P)
FO: source of beneficial fatty acids
• Omega-3 to meet consumer expectations
FM & FO cost
Source: Tacon & Metian (2008)
Alternatives to FM & FO: what
does the sea have to offer?
• Seafood processing by-products from
sustainably managed fisheries
• New business opportunities
Shrimp processing
30%
Residues (70%)
Recycled
Liquid
Solids
Photos: http://dailyfoodporn.wordpress.com/tag/nordic-shrimp/
Shrimp residue meal
Shrimp residue meal
70
% of DM
60
50
40
30
20
10
0
Protein
Lipid
Ash
Fiber
Proximate composition
P
Shrimp residue meal
7,0
Shrimp residue
% of DM
6,0
Herring
5,0
4,0
3,0
2,0
1,0
0,0
Arg Cys His Iso Leu Lys Met Phe Thr Try Val
EAA
Shrimp residue meal (SRM)
Body weight (g)
6
Commercial
SRM
4
2
0
Initial
Final
CONCLUSION
1. SRM:
•Candidate alternative to $$$ sources of
beneficial nutrients
2. Next steps
•Approval by regulatory agencies
LABORATORIES AND ANALYSIS SERVICES:
BIOCHEMICAL CHARACTERIZATION OF
MARINE PRODUCTS AND COPRODUCTS
Josée Boudreau, PhD, Marie-Hélène Thibault, PhD,
Claude Pelletier, M.Sc, Mathieu Ferron, M.SC
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MARINE PROCESSING COPRODUCTS
Over the years, our multidisciplinary team has developped an
expertise in the screening and characterization of several marine
processing coproducts such as:
Sea cucumber
• Mouth
• Anus
• Viscera
Lobster
Snow crab
• Head
Arctic shrimp
• Cephalothorax
• Shell
• Protein waste
• Hepatopancreas
• Shell
Herring
• Head
• Tail
• Viscera
• Milt
• Roe
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CHARACTERIZATION OF MARINE COPRODUCTS
In a first step, proximate analysis, using standard methods,
reveals the major constituents in the products under study
Immature herring roe
Herring milt
Crude
protein
18%
Crude
protein
24%
Humidity
70%
Crude fat
5%
Ash
1%
Crude fat
3%
Ash
2%
Humidity
77%
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CHARACTERIZATION OF MARINE COPRODUCTS
Each class of compounds can be probed further to gain knowledge
about the product at hand and the biomolecules it contains
Immature herring roe
Crude
protein
24%
Humidity
70%
Crude
fat
5%
Ash
1%
• Marine products are known to be
rich in phospholipids and
omega-3 fatty acids, as well as
fat-soluble biomolecules such as
vitamins (A, D, E) and pigments
• Lipids are therefore often one of
the main focuses in the study of
marine coproducts
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MARINE COPRODUCT LIPIDS
Each
Lipid classes
• Phospholipids • Wax esters
• Sterols
• Neutral vs
• Triglycerides
polar lipids
Rancidity/oxidation
• Free fatty acids (FFA)
• Peroxide value
• p-anisidine value
Fatty acid profile
• Omega 3
• Omega 6
• EPA, DHA
• Saturated
• Mono-, polyunsaturated
Other bioactive molecules
• Vitamins (A, D and E)
• Carotenoid pigments
(Astaxanthin, β-carotene, etc.)
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MARINE COPRODUCT PIGMENTS
Current projects in our laboratories include the detailed study and
characterization of astaxanthin, a widespread and commercially
relevant marine pigment, in shellfish processing coproducts.
Astaxanthin
For animals:
• Widely used in aquaculture for flesh
pigmentation improved fish growth
For humans:
• Powerful antioxidant
• Anti-tumor
• Anti-inflammatory
• Protects against cardiovascular
diseases and ulcers
• Treats neurodegenerative diseases
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ASTAXANTHIN ANALYSIS
Various chemical forms of astaxanthin naturally occur and
influence its biological properties and stability. Different analytical
methods are required to study these complex molecules.
Astaxanthin
1. Free or with fatty acid(s)
Free, monoester or diester
2. Geometrical isomers
All trans, 9-cis, 13-cis, etc.
3. Optical isomers
{R,R’}, {R,S’} (MESO), {S,S’}
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ASTAXANTHIN ANALYSIS
Current analytical capabilities in our laboratories include:
 Quantification of free, esterified and total astaxanthin
 Identification of geometrical isomers (all trans, 9-cis, 13-cis, etc.)
High performance liquid
chromatography (HPLC)
RAMAN and FT-Infrared
microspectroscopy
 Normal phase
 Reversed phase
 Chiral reversed phase
 Microscope for in situ imaging
 « Same spot » technology for
heterogeneous sample mapping
Work is in progress to develop a rapid, simple, in situ method for
the identification of astaxanthin optical isomers in different products
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Fishery coproduct as nutraceutics (natural
health products and functional foods):
Innovation based technology
Neurodegeneration
1. Anti-diabetes
2. Anti-inflammatory
3. Anti-obesity
Diabetes
Insulin resistance
Obesity
4. Neuroprotection
Inflammation
Valorization of Coproducts from
fish processing plants
 CZRI
 NRC- Halifax
 Purification
 Bioguided fractionation
 Analyses
 Purification
 Process development
 Adipocytes cell model (Obesity)
 Pilot scale unit
 NRC- Charlottetown
 Dalhousie University
 in vitro and in vivo diabetes
 in vitro and in vivo Alzheimer
model
 Bioguided fractionation
Jacques Gagnon
model
CZRI phases
• Animal feed
• Natural health
products (NHPs)
• Human food
products
Aquaculture
or marine
biomass
New,
added-value
products
• Fish cuttings
• Shrimp
• Sea cucumbers
Chemical
analysis
CZRI
Product
development
• Product formulation
• Product stability
• Market opportunities
• Natural product
chemistry extraction
• Chemical fractionation
• Chemical
characterization
Bio-analysis
• In vitro bioassays
• In vivo models
Lead
bioactives
• Scale up
• Food technology centers
• Partnering initiatives
Dr. Bob Chapman
Summary
• Extracts and protein hydrolysates prepared: ~100
• Focus on lead products that would be beneficial in
metabolics disorders (e.g. pre-diabetes, obesity), be
neuroprotective, or anti-inflammatory
•Shrimp extracts and herring milt hydrolysates gave
good activity for antidiabetic and neuroprotective
effects
•These should be further assessed by the
development of health supplement ingredients with
animal model of alzheimer’s disease and diabetes
Jacques Gagnon
Funding Agencies
• Atlantic
Canada Opportunities Agencies
(ACOA)- Atlantic Innovation Fund (AIF)
•New Brunswick Innovation Fund (NBIF)
•Industrial Partners: ex: Island Fishermen
Cooperative Association
•New Brunswick Department of Agriculture,
Aquaculture and Fisheries
Jacques Gagnon
Patent No.
Kind
WO 2014138920 A1
Date
Sep 18, 2014
Application No.
WO 2014-CA251
Date
Mar 13, 2014
What is in the pipeline
 Patent published… more to come with antidiabetic
activities of shrimp oil
 Market feasability study with shrimp oil in progress
 Animal model of diabetes and neurodegeneration with
shrimp oil and herring milt hydrolysate to confirm
positive effects are in progress
 Looking for a commercial receptor partner
 Data collection in animal models to support a human
clinical trial with shrimp oil (NHP)
 Purification process of shrimp oil for human use at lab
scale- with scaling up at industrial level
Jacques Gagnon
Questions?
THANK YOU