Core facilities Helse Sørøst
Opportunities to get high-technological
laboratory analysis without being an expert
Ivar Sjaastad
Board for CF OUS
Institute for experimental
medical research - OUS
Definition HSØ
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Gjør tilgjengelig, eller bidrar med analyser fra, særlig avansert og kostbart
vitenskapelig utstyr som ikke finnes allment tilgjengelig i forskningsmiljøene, og
legitimeres i stor grad av etterspørsel og brukermasse utenfor eier av fasiliteten
Krever spisskompetent personell for drift og service; spesialkompetansen må
dokumenteres f.eks gjennom dette personellets forskningsaktivitet
Kan unntaksvis være rene kompetanseplattformer (spesialkompetanse uten
avansert kostbart utstyr) når etterspørsel etter kompetansen er utstrakt og særskilt
viktig for forskningsaktiviteten – et eksempel på dette er for tiden computational
life science
Har påtatt seg en forpliktelse til å tilby avanserte/ høyspesialiserte analyser uten
automatisk å ha krav om medforfatterskap (men dette kan vurderes etter
Vancouverreglene når bidraget innebærer videreutvikling utover standard
analyseoppsett)
Status som regional kjernefasilitet innebærer forpliktelser til å betjene relevante
brukermiljøer i hele regionen
Goals CF HSØ
- Make high tech and costly methods available for all relevant
researchers
- Service, not necessarily co-authorship
- Substantial number of users outside CF research group
Why service? Funding of CF
Regional CF: 1-6 mill NOK annually for running costs
Access to CF HSØ
Researchers from relevant institutions within or outside HSØ, both national and
international, can have access.
Researchers from HSØ are given priority.
All CF are located to OUS.
CF HSØ
http://ous-research.no/corefacilities/
Overview of the CFs
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Approx 17 CF HSØ
Dynamic list
Information on webpages
TAKE CONTACT
Presented
Deep sequencing
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Genomic DNA sequencing
mRNA sequencing
smallRNA/microRNA sequencing
Epigenetics
– Chromatin immunoprecipitation sequencing
– DNA methylation
Ullevål
Dag Undlien
UiO
Kjetil Jakobsen
Radiumhospitalet
Leonardo Meza-Zepeda
Gene array for gene expression
and genotyping
• Gene expression level
• Genotyping
Radiumhospitalet
Leonardo Meza-Zepeda
Proteomics
• Mass spectrometry combined with modern
separation techniques.
– Protein identification
– Sequence determination
– Characterize post-translational modifications
– Protein-interaction analysis
– Peptide and protein quantification
Rikshospitalet
Gustavo de Souza
Presented
Bioinformatics
• Bioinformatics, including analysis of deep sequencing data,
protein structure etc.
• Access to usefull databases and web services
• The only competence CF
Radiumhospitalet
Eivind Hovig
Presented
Structure biology
• Structure determination of proteins using protein
crystallography or X-ray methodology
• High-energy X-ray radiation for X-ray crystallography
at the ESRF synchrotron in Grenoble
• Modelling of proteins and their interactions.
• 3D modelling to predict effects of mutations or
polymorfisms.
Rikshospitalet
Bjørn Dalhus
Flow cytometry
• Flow cytometry analysis
(cells, bacteria,
chromosomes, beads and
others)
• Cell characterization
• Cell sorting
Rikshospitalet
Fridtjof Lund-Johansen
Radiumhospitalet
Trond Stokke
Ullevål
Hans Christian Dalsbotten Aass
Confocal and superresolution
microscopy
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Assistance or access after training
Service
Fixed material or live cells
gSTED (RH) and 3D-SIM (Rad)
(PALM/dSTORM soon at Rad)
Rikshospitalet
Frode Jahnsen / Stig Ove Bø
Radiumhospitalet
Harald Stenmark
Ullevål
William Louch
Electron microscopy
• Ultrahigh resolution structure analysis of
cells and tissue.
Radium
Andreas Brech
Rikshospitalet
Finn Reinholt
Transgenic animal models
• Establishes transgene and mutant mice / rats
• Genotyping
• Characterization of histological phenotype and
behavior analysis.
Phenotyping
Rikshospitalet
Knut Thomas Dalen
Preclinical MRI
• Non-invasive high resolution cardiac imaging of mice
and rats
• Anatomical, functional and dynamic imaging
• Longitudinal in vivo studies
Ullevål
Ivar Sjaastad
Radiumhospitalet
Kristian Berg
Large animal research
• Competence in planning and performing advanced
large animal research (pigs)
• Surgical facilities for large animals:
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Cardiovascular flow and pressure measurements
Echocardiography
Angiography
Electrophysiology
Rikshospitalet
Håvard Attramadal
Ullevål
Ole Sejersted
Intervention center
• High tech facility for clinical and pre-clinical research.
– Develop new procedures, methods and technology.
Rikshospitalet
Erik Fosse
CF at OUS / HSØ
Opportunities to get high-technological
laboratory analysis without being an expert
http://ous-research.no/corefacilities/