Meiosis is the basis of sexual reproduction. Sexual Reproduction: a method of reproduction involving two parents, producing offspring that are genetically different from each other, either parent, and from other members of their species. Sexual reproduction involves uniting specialized cells called Gametes. In animals, male gametes are called sperm and female gametes are called eggs. Fertilization occurs when the gametes unite, with the sperm penetrating an egg cell. The process of fertilization results in a cell called a Zygote. The Zygote undergoes cell division and mitosis and develops into an Embryo. The embryo grows continuously to form a new organism. Diploid number (2n): body cells (all cells excluding sperm and egg) have two sets of chromosomes. The diploid number for humans is 46 (2 x 23). Humans inherit one set of 23 from their male parent and one set of 23 from their female parent. Haploid number (n): gametes (sperm and egg cells) have only one set of chromosomes and are termed haploid. Human gametes have one set of 23 chromosomes. So, the haploid number for humans is 23. During fertilization, a haploid (n) sperm cell unites with a haploid (n) egg cell to form a diploid (2n) zygote. n + n = 2n Meiosis: process of producing gametes (sperm and egg cells) with the haploid (n) number of chromosomes. Meiosis occurs in the male and female sex organs (respectively the testes and ovaries). Meiosis involves two rounds of cellular division: 1. Meiosis I 2. Meiosis II Homologous Chromosomes: are pairs of chromosomes that are the same size, shape and have genes that are in the same locations. Meiosis 1. 2. 3. 4. I involves: Prophase I Metaphase I Anaphase I Telophase I Homologous chromosomes pair up. Homologous equator. chromosomes pair up at the Homologous chromosomes separate and are pulled towards opposite poles (ends) of the cell. Homologous pairs have moved to opposite ends of the cell and the cell begins to divide. Interkinesis: is the stage between cell divisions. The cells grow and make various proteins. The end products of Meiosis I are two diploid (2n) daughter cells. These cells now go on to enter Meiosis II. Meiosis 1. 2. 3. 4. II involves: Prophase II Metaphase II Anaphase II Telophase II There is one chromosome of the homologous pair in each cell. Chromosomes of the cell). line up at the equator (middle Half of each X-shaped chromosome is pulled apart towards opposite poles (ends) of the cell. A nucleus forms around each set of chromosomes and the cell begins to divide to produce 4 gametes. In males, after meiosis II, four sperm cells are formed. In females, during meiosis, there is an unequal division of cytoplasm. Following meiosis II, 3 cells will disintegrate leaving only 1 egg. Mitosis Meiosis Types of Cells Body Cells Sex Cells (sperm and eggs) Number of Daughter Cells produced 2 4 Amount of Genetic Material in each daughter cell Same number of chromosomes (2n) in each cell. Each daughter cell is diploid. Half the number of chromosomes (n) in each cell. Each gamete (sex cell) is haploid. Sexual Sexual reproduction involves two parents. Reproduction does not necessarily require sexual intercourse. Fertilization 1. 2. may be: Internal Fertilization – sperm cells are deposited inside the female’s body where they meet an egg cell. Requires sexual intercourse. External Fertilization- sperm and egg cell unite outside the bodies of parents. This is common in animals that live in water. Mosses • External fertilization occurs in mosses. Water is needed to transport gametes, allowing sperm cells and egg cells to unite. Male and female sex organs develop on the end of stems or branches. Asexual reproduction may also occur by spore production. • • • • • • • Flowering Plants Internal fertilization occurs in flowering plants. The process of Pollination occurs. Male gametes are formed in special cases called Pollen. Pollination is the transfer of pollen to the female part of the flower. The male reproductive organ in a flower is called the Stamen. The female reproductive organ in a flower is called the Pistil. Pollination and fertilization occurs at the female reproductive organ, at the pistil. The pollen lands on the pistil and sperm are delivered to the egg cells. The fertilized egg becomes a seed. The seed protects the developing embryo. Insects The life cycle of insects involves metamorphosis. Metamorphosis is the change in an individual's form as it develops. Incomplete Metamorphosis: involves subtle (minor) changes through 3 life cycle stages: 1. 2. 3. Egg Nymph (smaller immature version of adult) Adult Grasshoppers go through Incomplete Metamorphosis. Complete Metamorphosis: a change in the form of an insect as it matures, where the adult is completely different than the larval stage. The four common stages of complete metamorphosis, as seen in the butterfly are: 1. Egg 2. Larva (caterpillar) 3. Pupa 4. Adult Asexual Sexual 1 2 Gametes (eggs or sperm) None (cell divides) 2 (egg cell and a sperm cell unite to form a zygote) Variation (difference) in offspring Lesser (all offspring identical) Greater (genetic diversity) Amount of Energy required Lesser Greater Parental care Lesser Greater Mitosis or Meiosis? Mitosis Meiosis # of parent cells Genetic conditions that cannot be solved using current scientific & technological knowledge include: 1. Down Syndrome Result of an extra 21st chromosome Individuals have characteristic facial features and a shorter stature. They may be prone to heart defects and other diseases such as Alzheimer’s and leukemia. Allderdice Syndrome 2. Unique to Sandy Point, NL. An increase in genetic birth defects was noticed due to geographic isolation. Result of a mutation on a single maternal chromosome. 31% chance of developing the syndrome. 3. Cystic Fibrosis Chronic disease that causes all bodily mucous to become thick, sticky and difficult to expel. People with CF are prone to respiratory issues as well as digestive issues amongst many others. Caused by a gene mutation on a single chromosome. An individual only needs one gene to prevent it but most people have two (one on each homologous chromosome). So the individual must inherit an absence of both genes to develop disease. Our understanding of genetics has changed over time as new technologies have become available. New technologies have made it possible to get a better look at genes and their influence on traits. In the mid 1800’s, Gregor Mendel experimented with inherited traits in pea plants, including color and shape. Mendel’s studies of pea plants demonstrated that traits were inherited from one generation to the next. Mendel’s studies suggested the involvement of “dominant” and “recessive” factors in the transmission of traits from parents to offspring. Dominant traits are always expressed, however recessive traits are not always expressed. A more clear understanding of genes came about when Francis Crick and James Watson, described the structure of DNA in 1953. Crick and Watson showed that DNA is an organization of genes in a double helix shape, like a twisted ladder. Specific base pairing on this ladder helped to explain how DNA could replicate (copy itself). This development also helped to explain how and why mutations occur. In the Human Genome Project, scientists around the world collaborated for about 20 years to identify every gene in human DNA, mapping the human genome. The Human Genome Project made a sort of map that can be used to search for and identify particular genes. The Human Genome Project has provided information into how and why various genetic diseases come about. Genetic Engineering: is biotechnology that deals with the manipulation of the genome. Scientists have figured out how to cut a gene out of one DNA strand and place it into another. The ability to recombine DNA (recombinant DNA) has made significant contributions to food and medicine. Plants and animals have improved through recombinant DNA technology. Such organisms are labeled as Genetically Modified Organisms (GMOs). This plays an important role in the agricultural industry, specifically crop production. Genes have been altered to produce plants that are resistant to colder temperatures, chemicals and disease. Genetic engineering has been used to produce organisms with desired traits. Scientists use recombinant DNA technology to produce drugs and provide human gene therapy. Recombinant DNA technology is being used to help Diabetes patients, in which the correct human gene for insulin production, is placed within the genome of a bacterium. The bacterium then produces insulin which can be used as medicine. Humans who lack a specific gene or who have a defective gene can have a healthy, functioning gene inserted into their DNA. This is human gene therapy.