Chapter 5 Tissues – Epithelial 1 Introduction • Similar cells with a common function are called tissues. • The study of tissues is called histology. • There are four (4) primary or major tissue types: 1. 2. 3. 4. Epithelial Tissue (covering/lining; ET) Connective Tissue (support; CT) Muscle Tissue (movement; MT) Nervous Tissue (control; NT) 2 Nervous tissue: Internal communication • Brain, spinal cord, and nerves Muscle tissue: Contracts to cause movement • Muscles attached to bones (skeletal) • Muscles of heart (cardiac) • Muscles of walls of hollow organs (smooth) Epithelial tissue: Forms boundaries between different environments, protects, secretes, absorbs, filters • Skin surface (epidermis) • Lining of GI tract organs and other hollow organs Connective tissue: Supports, protects, binds other tissues together • Bones • Tendons • Fat and other soft padding tissue Figure 4.1 Epithelial Tissue Epithelial Tissue (Epithelium) Two main types (by location): 1. Covering and lining epithelia • On external and internal surfaces o o body (i.e. epidermis) and ventral cavity organs (i.e. visceral serous membranes) 2. Glandular epithelia • Secretory tissue in glands o internal spaces (i.e. lumen of the intestine), o line body cavities (i.e. parietal membranes), o line ducts of exocrine glands (i.e. sweat glands). Functions of Epithelial Tissue • • • • Protection Absorption Filtration Secretion 5 Characteristics of Epithelial Tissue 1. Cells exibit polarity—apical (upper, free) and basal (lower, attached) surfaces – Apical surfaces may bear microvilli (e.g., brush border of intestinal lining) or cilia (e.g., lining of trachea). – Apical surfaces always have a free space, which opens to the outside or to an internal space (lumen) – Noncellular basal lamina of glycoprotein and collagen lies adjacent to basal surface Characteristics of Epithelial Tissue 2. 3. 4. 5. 6. 7. Cellularity - cells are in close contact with each other with little or no intercellular space between them. Supported by a connective tissue - reticular lamina (under the basal lamina) at the basal surface, both the epithelial tissue and the connective tissue contribute to the basement membrane Avascular (no blood supply). Innervated (supplied with nerves) Rely on diffusion and underlying connective tissue for nutrients and O2 High rate of regeneration 8 Cellularity - Intercellular Junctions Tight junctions • Close space between cells • Located among cells that form linings • lining cells in small intestine • kidney tubules • blood-brain barrier Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Desmosomes • Form “spot welds” between cells • Found in tissues that undergo repeated episodes of tension and stretching • skin, heart, uterus Cell membrane Tight junction Cell membrane Desmosome Cell membrane Gap junctions • Tubular channels between cells • Located in cardiac muscle cells • cardiac muscle cells • digestive smooth muscle cells Gap junction 9 Basement Membrane: The Basal Lamina • Noncellular supporting sheet between the epithelium and the connective tissue deep to it • Consists of proteins secreted by the epithelial cells • Functions: – Acts as a selective filter, determining which molecules from capillaries enter the epithelium – Acts as scaffolding along which regenerating epithelial cells can migrate • Basal lamina and reticular layers of the underlying connective tissue form the basement membrane Classifications of Epithelia • First name of tissue indicates number of layers – Simple – one layer of cells – Stratified – more than one layer of cells - Pseudostratified- tissue appears to be stratified, but all cells contact basement membrane so it is in fact simple Classifications of Epithelia • Last name of tissue describes shape of cells – Squamous – cells wider than tall (plate or “scale” like) – Cuboidal – cells are as wide as tall, as in cubes - Columnar – cells are taller than they are wide, like columns Epithelial Tissue • Simple squamous: • Simple cuboidal: • Single layer of flat cells • Substances pass easily through • Line air sacs • Line blood vessels • Line lymphatic vessels • Single layer of cube-shaped cells • Line kidney tubules • Cover ovaries • Line ducts of some glands Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Free surface of tissue Lumen Nucleus Simple squamous epithelium Basement membrane Basement Free surface of tissue Nucleus Simple cuboidal epithelium Connective tissue Connective tissue (a) (b) b,d: © Ed Reschke (a) (b) b: © The McGraw-Hill Companies, Inc./Al Telser, photographer 13 Simple Squamous Epithelium Figure 4.3a Simple Cuboidal Epithelium Figure 4.3b Epithelial Tissue • Simple columnar: • Pseudostratified columnar: • Single layer of elongated cells • Nuclei usually near the basement • Membrane at same level • Sometimes possess cilia • Sometimes possess microvilli • Often have goblet cells • Line uterus, stomach, intestines • Single layer of elongated cells • Nuclei at two or more levels • Appear striated • Often have cilia • Often have goblet cells • Line respiratory passageways Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Cilia (free surface of tissue) Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Cytoplasm Mucus Goblet cell Nucleus Nucleus Cytoplasm Basement membrane Microvilli (free surface of tissue) Connective tissue Goblet cell (a) (b) Basement membrane b: © The McGraw-Hill Companies, Inc./Dennis Strete, photographer Connective tissue (a) (b) 16 b: © The McGraw-Hill Companies, Inc./Al Telser, photographer. Simple Columnar Epithelium Figure 4.3c Pseudostratified Ciliated Columnar Epithelium Figure 4.3d Stratified Epithelial Tissue • • • • Contain two or more layers of cells Regenerate from below Major role is protection Are named according to the shape of cells at apical layer Stratified Squamous Epithelium • Specific types – Keratinized – contain the protective protein keratin • Surface cells are dead and full of keratin – Non-keratinized – forms moist lining of body openings • Function – Protects underlying tissues in areas subject to abrasion • Location – Keratinized – forms epidermis – Non-keratinized – forms lining of esophagus, mouth, and vagina Epithelial Tissue • Stratified squamous: • Stratified cuboidal: • Many cell layers • Top cells are flat • Can accumulate keratin • Outer layer of skin • Line oral cavity, vagina, and anal canal • 2-3 layers • Cube-shaped cells • Line ducts of mammary glands, sweat glands, salivary glands, and the pancreas Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Free surface of tissue Stratified cuboidal epithelium Nucleus Squamous cells Lumen Free surface of tissue Basement membrane Connective tissue (a) (b) Layer of dividing cells Basement membrane b: © The McGraw-Hill Companies, Inc./Al Telser, photographer. Connective tissue (a) (b) b: © The McGraw-Hill Companies, Inc./Al Telser, photographer 21 Stratified Squamous Epithelium Figure 4.3e Stratified Cuboidal Epithelium • Description – generally two layers of cube-shaped cells • Function – protection • Location – Forms largest ducts of sweat glands – Forms ducts of mammary glands and salivary glands Epithelial Tissue • Transitional: • Stratified columnar: • Many cell layers • Cube-shaped and elongated cells • Line urinary bladder, ureters, and part of urethra • Top layer of elongated cells • Cube-shaped cells in deeper layers • Line part of male urethra and part of pharynx Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Free surface of tissue Unstretched transitional epithelium Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Lumen Basement membrane Free surface of tissue Stratified columnar epithelium (a) Underlying connective tissue (b) Basement membrane Free surface of tissue Stretched transitional epithelium Connective tissue (a) (b) Basement membrane Underlying connective tissue b: © The McGraw-Hill Companies, Inc./Al Telser, photographer (c) (d) b,d: © Ed Reschke 24 Stratified Columnar Epithelium • Description – several layers; basal cells usually cuboidal; superficial cells elongated • Function – protection and secretion • Location – Rare tissue type – Found in male urethra and vas deferens, largest ducts of salivary glands, nasopharynx Transitional Epithelium Figure 4.3h Glandular Epithelium • A gland is one or more cells that makes and secretes an aqueous fluid •Composed of cells that are specialized to produce and secrete substances • There are two (2) types: • Endocrine glands no contact with exterior of body; ductless; produce hormones (pituitary, thyroid, adrenals, pancreas) • Exocrine glands o Exocrine glands classified either by structure or by the method of secretion o Classified by structure 27 Endocrine Glands • Glands that do not have ducts or tubules and whose secretions are distributed throughout the body • Produce and secrete hormones into the bloodstream or the lymphatic system • Part of a complex, biochemical network known as the endocrine system 28 Exocrine Glandular Epithelium • Unicellular exocrine gland: • Composed of one cell • Goblet cell • Multicellular exocrine gland: • Composed of many cells • Sweat glands, salivary glands, etc. • Simple and compound 29 Unicellular Exocrine Glands (The Goblet Cell) • Goblet cells produce mucin • Mucin + water mucus • Protects and lubricates many internal body surfaces Multicellular Exocrine Glands • Classified by structure (branching & shape) of duct • Classified on the basis of types of ducts or mode of secretion • Types of ducts – Simple: ducts with few branches – Compound: ducts with many branches • Mode of Secretion – Merocrine secretion – secretory vesicles released via exocytosis (saliviary glands) – Apocrine secretion – apical portion of the cell is lost, cytoplasm + secretory product (mammary glands) – Holocrine secretion – entire cell is destroyed during secretion (sebaceous gland) May also be classified by types of secretions from exocrine glands • Serous – mostly water but also contains some enzymes – Ex. parotid glands, pancreas • Mucous – mucus secretions – Ex. sublingual glands, goblet cells • Mixes – serous & mucus combined – Ex. submandibular gland Types of Glandular Secretions • Merocrine Glands • Apocrine Glands • Fluid product • Salivary glands • Pancreas gland • Sweat glands • Holocrine Glands • Cellular product • Portions of cells • Mammary glands • Ceruminous glands • Secretory products • Whole cells • Sebaceous glands Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Intact cell Secretion Pinched off portion of cell (secretion) Disintegrating cell and its contents (secretion) New cell forming by mitosis and cytokinesis (a) Merocrine gland (b) Apocrine gland (c) Holocrine gland 33 Structural Types of Exocrine Glands Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Tissue surface Duct Secretory portion Simple tubular Simple branched tubular Compound tubular Simple coiled tubular Compound alveolar Simple branched alveolar 34 Exocrine Vs. Endocrine Glands • Endocrine Gland Characteristics: – Ductless glands – Secrete substances directly into bloodstream – Produce molecules called hormones Which is Which?