General Background Tetra Environment Characteristics ASSET Labs and of Tetra Tetra ASSET Lab Equipment 101 ASSET 101 100 100 100 100 100 100 200 200 200 200 200 200 300 300 300 300 300 300 400 500 400 400 400 400 400 500 500 500 500 500 Final Jeopardy Tetrahymena is a ______ cell, meaning it has a membrane-bound nucleus and organelles. What is a Eukaryote? This is the Kingdom that Tetrahymena belong to. What is Protista? This is the term used to describe something that DOES NOT cause disease. It is also one of the reasons Tetrahymena is a safe organism to use in classrooms. What is non-pathogenic? Like other living things, Tetrahymena perform these three basic functions… What are eat, reproduce, and respond to their environment? In 2009, this award was given to scientists doing research on telomeres using Tetrahymena. What is the Nobel Prize? Tetrahymena are found mostly in this type of water… What is fresh water? This is Tetrahymena’s food of choice, another reason why they are an excellent classroom organisms for non-sterile environments. What are bacteria? Like all organisms, Tetrahymena ___________, for limited resources in their environment. (Charles Darwin identified this as a basic biological concept) What is compete? Depending on environmental conditions, certain strains of Tetrahymena can go _______, meaning they eat their own species. What is Cannibalistic? When Tetrahymena go cannibalistic, the cannibals form one of these, which means, “Big Mouth”. What is a macrostome? Tetrahymena belong to this group of Protozoans, meaning they have cilia. What are Ciliates? This is the term used to describe how Tetrahymena eat. It means “to devour” a particle when feeding. What is Phagocytosis? “Fat”, “Balloon” and “Mouthless” are three different ______________ that are expressed when Tetrahymena are exposed to increased temperature. (37 degrees C) What are Mutations? One of the ASSET Modules allows student to create microcosms where they mix one of Tetrahymena’s favorite bacteria with the Tetrahymena. Bacteria that are able to survive by filling different niches produce visibly different colonies. The selection pressure causing this is? What is Predation? Humans have 2 sexes. How many sexes do Tetrahymena have? What are Seven (7)? These are structures found on the outside of Tetrahymena used for both movement and feeding. What are Cilia? This is the shape that Tetrahymena form when they are sexually reproducing. Also, a common term for a throwing device traditionally use by Australia’s Aboriginal people who use them for hunting. What is a boomerang? These organelles can be seen clearly under a microscope during the Phagocytosis and Osmosis Labs. Their role is to fill-up with extra water and pump it out of the cell to maintain homeostasis. What are contractile vacuoles? In the Field Research and Bioinformatics Lab, your students can extract Tetrahymena’s ________ and compare it to others to identify whether it is from a currently known species of Tetrahymena or a new species. What is DNA ? This refers to an organism’s ability to change directions in response to chemicals in its environment. Also, the name of one of the ASSET Labs. What is Chemotaxis ? These devices ship with many of the ASSET Lab Modules, they are designed to precisely measure, in microliters, the volume of a substance. What are micropipettes? These are the types of microscopes, with two lenses, you will need to use when observing Tetrahymena in your classroom. What are compound light microscopes? When culturing Tetrahymena in your classroom, it is important to use ________ technique, which is why we provide you with pipettes that are individual wrapped. What is STERILE technique? We lend these devices (standard and mini sizes), which allow you to spin down cells using centripetal acceleration. What is a Centrifuge (Micofuge)? These devices allow students to work in groups and all be engaged in watching what is going on under the microscope. They are a great technological tool to integrate into your science classroom! What is a Digital Camera (ASSET uses Moticams)? When asking to borrow an ASSET Lab Module, you must first __________ on our website? What is to register (create an easy to remember user name and password)? These two fantastic people started the ASSET Program at Cornell University. Who are Donna Cassidy-Hanley, and Ted Clark? When borrowing an ASSET module, we ask for this many weeks notice. What are Two Weeks? PLEASE! When your ASSET Kit arrives, what is the first thing you should do after opening it? What is find your Tetrahymena shipping tube and make a Stock Culture of it using the falcon tube provided? Three (3) days before running a lab with students, you will need to make “working cultures” of Tetrahymena in the provided sterile petri plates, HOW LONG do these plates generally stay viable to use in experiments? What are 3-4 days? (Plates start to crash on about Day 5. Plan to make fresh plates if you are using with other classes later in the week.) Category: Cellular Biology How much will you wager? Tetrahymena have a ______, that contains 5 pairs of chromosomes with about the same number of genes as humans have and divides mitotically. Tetrahymena also have a ________, which have about 300 chromosomes and instead of dividing mitotically, it elongates, constricts and divides to pass on to the daughter cells. (NEED BOTH BLANKS for Full Points) What are a micronucleus and a macronucleus ? Tetrahymena have 2 nuclei! COOL!