Cell Theory • All organisms are made up of one or more cells • The cell is the basic unit of structure and the function of all living things • All cells arise from previously existing cells Levels of Organization Cells; What are they Good For? • • • • • • Getting food Using food for Energy Transportation Growing Reproducing Elimination of Waste 2 Types of Cells Prokaryotic Eukaryotic • No Nucleus • Circular DNA • No membrane bound organelles • Nucleus • Linear DNA • Membrane bound organelles Animal vs. Plant Cells Animal Cells Plant Cells Cell Structure The Cell Membrane • Controls the movement of materials in and out of the cell All cells have a cell membrane The Cell Wall provides structure and support. All cells EXCEPT animals have a cell wall The Nucleus holds the DNA • What does DNA do? All cells EXCEPT bacteria have a nucleus The Nucleolus is a region of the nucleus where ribosomes are made All cells EXCEPT bacteria have a nucleolus The Rough Endoplasmic Reticulum (RER) • Is studded with ribosomes. • Helps fold proteins for export or transport • Packages proteins in vesicles to send to the Golgi Apparatus All cells EXCEPT bacteria have a rough endoplasmic reticulum The Smooth Endoplasmic Reticulum (SER) • Processes and packages polysaccharides and lipids in vesicles. All cells EXCEPT bacteria have a smooth endoplasmic reticulum Ribosomes are where proteins are formed. •They consist of two sub-units (large & small) All cells have ribosomes The Golgi Apparatus • Receives proteins in vesicles from the RER • Adds tags to proteins to mark them for delivery and then sends them out for shipment. All cells EXCEPT bacteria have a golgi apparatus The Mitochondria is the site of cellular respiration • Where sugar is turned into ATP, and energy is released All cells EXCEPT bacteria have mitochondria The Chloroplast is the site of photosynthesis • Where sunlight is transformed into sugar Only plant cells have chloroplasts The Cytoskeleton creates internal structure and allows for movement internally and externally. Lysosomes are specialized vesicles made from the golgi •They break down old organelles, foreign materials, bacteria and viruses All cells EXCEPT bacteria have lysosomes Vacuole • Membrane-bound sacs that aid in… – Digestion – Water pressure – Waste release • All cells have vacuoles, however, their function is dependent on the type of cell Inner Life of the Cell by: Harvard University Narrated Version – Click Here Cellular Transportation The Cell Membrane • All cells have a cell membrane – It is a thin, flexible barrier surrounding the cell • – provides protection and support. Has the ability to change shape. • Why would this be important? Cell Membrane • It is selectively permeable – It controls what enters and leaves the cell. Parts of the Cell Membrane • The cell membrane is made up of lipids (AKA fats). – Containing two different parts • • water-loving head (hydrophilic) 2 water-hating tails (hydrophobic) Parts of the Cell Membrane • These lipids join together as a lipid bilayer (i.e. 2 layers) in water. – These layers form an asymmetrical, closed circle. Parts of the Cell Membrane • What do you think would happen if there was a tear in the bilayer? • How do you think temperature (too hot; too cold) might affect the bilayer? Parts of the Cell Membrane • The bilayer is also made up of different membrane proteins. – They aid in the transport of molecules across the membrane (transmembrane proteins) Parts of the Cell Membrane • Some have carbohydrate chains – They help protect the membrane surface – They play an important role in cell-cell recognition. – What are some examples of cell to cell recognition? Membrane Functions • Diffusion – This is the movement molecules from regions of higher concentration to regions of lower concentration Brining your Chicken • What is “Brining”? – The brining process forces water into the muscle tissues of the meat – What science process is occurring here? • Why would we want to do this with chicken? – The resulting water in the muscle tissues will make the meat more moist and tender. Membrane Functions (cont.) • Osmosis – The diffusion of water through a membrane. – Movement of water from a region of high water concentration to a region of lower water concentration through a semi permeable membrane. Osmosis Membrane Functions (cont.) • Passive transport – Diffusion occurs across a membrane without the use of energy. 1. Simple Diffusion a) b) Occurs through channel proteins or pores Dissolves into bilayer and diffuses across membrane 2. Facilitated Diffusion a) Needs help of a carrier protein Membrane Functions (cont.) • Active transport – Movement of molecules across a membrane with the use of energy. – Molecules are going against normal tendencies. – This occurs with the help of a carrier protein. Membrane Functions (cont.) Membrane Functions (cont.) Activity time The Hungry Cell •How do cells receive large molecules? –Such as proteins, food, etc… •Work in pairs •Materials: –plastic shopping bag (the cell), –a pair of scissors, –a piece of string, –several pieces of “food” (chickpeas). “Hungry Cell” Rules You must get the food into the cell, BUT you must follow these rules: • The food must enter through a solid part of the bag – you can’t just drop it into the bag • The inside of the bag may NOT be directly open to the outside environment – In other words, you can’t work with the bag turned insideout, because neither can cells. • When the food enters the “cell” all the pieces MUST stay together and must be able to move around together inside the cell. • You ARE allowed to work with your hands inside the “cell”. Endocytosis • Cells take in macromolecules – What are some examples? Exocytosis • Materials leave cell – vesicles fuse with cell membrane and release material