Inheritance: Mendel’s Experiments Ideas on Inheritance Before Mendel Pangenesis Particles in the body are transmitted to the reproductive cells Blending Black horse X White horse =Gray horse Homunculus (small human) Spermists believed a small fully formed human was inside the sperm and the female contributed nothing but her womb! Gregor Mendel (1822-1884) Augustinian monk Studied the inheritance of traits in over 20,000 pea plants Kept meticulous notes on his data No knowledge of DNA or chromosomes Father of genetics His genius was not recognized until after his death The Lingo of a Geneticist Gene The unit of heredity (ex. Gene for flower color) Alleles Alternate forms of a gene (ex. Purple or white flower color) Dominant vs. Recessive One allelic form “masks” the presence of another ex. Purple flowers are dominant over white flowers (white is recessive) Dominant =capital letters Recessive =lower case letters Pn and Fn Parental and Filial generations More Lingo Genotype vs. Phenotype Genotype is what alleles an individual has (ex. One purple flower allele and one white flower allele) Phenotype is what the individual looks like (ex. Purple flowers) Homozygous vs. Heterozygous Homo =same Hetero =different True-breeding Phenotype of parent is always seen in offspring The phenotype counterpart to the genotype term “homozygous” If an individual has a homozygous genotype, it will be true breeding Monohybrid vs. Dihybrid A monohybrid cross looks at just one trait in parents who are hybrids A dihybrid cross looks at more than one trait in parents who are hybrids Hybrid is another way of saying “heterozygous” Monofactorial Inheritance A.k.a. Mendelian traits Inheritance of a trait is determined by one gene Does not really exist Virtually all traits are affected to some degree by other genes and by environmental factors Pea Plant Traits Flower color Purple (P) White (p) Mendel’s Experiments (Pea Sex) The Gene for Stem Length The tall allele is dominant (T) A true-breeding tall plant is homozygous dominant (TT) The short allele is recessive (t) A true-breeding short plant is homozygous recessive (tt) Homozygous (True-breeding) Dominant Tall plant X Tall plant X All offspring will be tall Homozygous (True-breeding) Recessive Short stem plant X Short stem plant X All offspring will be short Non-True-Breeding Tall plant X Tall plant X Some offspring are tall Some offspring are short Monohybrid Cross –Stem Length Parental generation (P1) Tall X Short (Both true-breeding) F1 All Tall (Non-true-breeding) a.k.a. hybrids F2 ¼ Short ¾ Tall Truebreeding Tall Truebreeding Short Non-truebreeding Tall Monohybrid Cross -Flower Color Mendel’s Law of Segregation Elementen separate into gametes We now know “elementen” as genes Each individual carries 2 alleles for every gene One on each chromosome One allele on the chromosome from mom and one allele on the chromosome from dad Possibilities for any given gene Homozygous Dominant (AA) (True breeding) Heterozygous (Aa) (Non-true-breeding) Homozygous Recessive (aa) (True-breeding) Gametes carry just ONE allele for each gene Possibilities are A or a Our Old Friend Meiosis Germline Cells Phenotypic ratio The genotypic ratio is 1:2:1 The Punnett Square Used to determine phenotypic and genotypic ratios Represents possible alleles in gametes and how gametes may combine Probability of having an offspring with “tt” is the same (1/4) for every pregnancy/fertilization. #s are ratios, not # of offspring Example True-breeding (homozygous) dominant Dominant and Recessive Traits A dominant trait requires only one allele in order for the dominant trait to be expressed There MUST be 2 recessive alleles Flower color present in order for a recessive trait to be expressed (phenotype) Purple (P) White (p) Examples Ex. 1: True-breeding (homozygous) recessive Ex. 2: Homozygous dominant X heterozygous Ex. 3: Monohybrid (heterozygous) cross Genotype vs. Phenotype The Test Cross X Example Unknown X Homozygous Recessive a.k.a “the test cross” Autosomes vs. Sex Chromosomes Autosomal traits are found on non-sex chromosomes so (they are not found on the X or Y sex chromosomes) Modes of Inheritance Autosomal dominant inheritance If a child exhibits the trait (phenotype) at least one of the parents also exhibits the trait (phenotype) Autosomal recessive inheritance Heterozygotes are carriers (genotype) and do not exhibit the trait (phenotype) Autosomal Dominant Autosomal Recessive Straight Hair as an Example Autosomal Recessive Phenylketonuria as an Example Mendel’s Conclusions There are discrete units of inheritance (elementen) We now call these genes Offspring inherit a unit (gene) for each trait from each parent Different units (genes) assemble independently in the gametes