I Can’t Believe The Hype – Beauty Products With Plant Stem
Cells by Aaron Saenz on June 30th, 2010
- Claims surrounding stem cell treatments are starting to get
downright ridiculous
- Various cosmetics lines have begun to market their goods as
containing the benefit from plant stem cells
- Many claims that these plant based stem cells will stimulate
human stem cell growth and promote healthier younger looking
skin
- Plant stem cells as age-defying makeup creams? Seriously?
Have we reached the point where we’re so enraptured with the
possibility of human stem cell technologies that we’ll buy
anything with ’stem cells’ in the product name?
- Are indispensable for growth and development in
both animals and plants
- Have two properties
- Self-renewal : the ability to go through
numerous cycles of cell division in an undifferentiated
state
- Differentiation : the capacity to differentiate
into other cell types
- May be able to repair or replace damaged tissue
♦ Have two basic types
♦ Embryonic stem cells (pluripotent)
- are derived from the inner cell mass of
an early stage embryo
- are abundant in embryo and will give rise
to numerous organs, tissues and cells in the body
♦ Adult or somatic stem cells (multipotent)
- are undifferentiated among differentiated
cells in a tissue or organ
- generate all the cell types of the organ
from which they originate – potentially regenerating
the entire organ from a few cells
♦ Are responsible for refreshing tissues and
compensating for cellular losses and are
susceptible to degradation that comes with
age
♦ Are rare and constantly lost in the tissues
to make the tissues be less able to grow,
maintain and repair
♦ Growth may be uncontrollable and
cancerous cells may reproduce causing
tumors
Normal
Without C/EBP-beta
Differntiated cells
Stem cells
Stem cells
 Epithelial skin stem cells in basal layer of
epidermis and hair bulge stem cells in hair folloicle
are basically found
 Epithelial skin cells are only capable of a certain
number of cell divisions and then die off – they
are shed constantly and require continuous
duplication of new cells
 Partly become fibroblasts responsible for
collagen, elastin and hyaluronic acid production
A loss is detrimental to the skin (one ages skin
stem cell division diminishes dramatically causing
in wrinkles and thinned skin)
● Have the ability to regenerate the whole plant
(any kind of cell or tissue)
● Have a much longer life
● Have a built-in mechanism that, which causes
the cell to ‘self destruct’ if damage to the DNA
is detected and defective DNA will not be passed
on
● Cannot make damaged or cancerous cells
reproduce themselves
● A callus tissue is formed
when a plant is wounded
● The normal differentiated
cells in the callus tissue go
back into a dedifferentiated
form and become stem
cells
● Mechanism is controlled
by different plant hormones
(auxins)
Stem cells can make identical copies
of
themselves
as
well
as
generate
Day 2
Day 3
Day 6
specialized cells
Stem cells
Stem cells grown
into filamants
Progenitor cell (stem cell)
Dead cells
Stem cells
Development into protonemal colony
Transient amplifying cell
Differentiated cell
Reprogramming of P. patens leaflet
cells into stem cells and subsequent
protonemata development
- Have an essential role in coordination of many growth
□ Contain compounds that are beneficial for human
and behavioral processes in the plant life cycle
health and beauty. They are
- The important ones include 4-chloro-indoleacetic acid,
 vitamins, minerals, oligoelements, growth
phenylacetic acid and indole-3-butyric acid
enhancing hormones (e.g., auxins, gibberellins),
-cytokinins,
May induce
the
cell
differentiation
and
regeneration
nucleic acid and enzymes essential for plant
of
the vascular tissues when plant is wounded
growth
- Provide
theinteresting
ability to regulate
protein synthesis
 many
plant substances
(e.g., flavonoids)
(evidences
showed multi-functional
the potential toand
promote
with new, powerful
specialcollagen
properties
and
e.g.,elastin)
detoxifying, UV stress protection
-□Only
found
in
plant
stem
cells
Potentially cope with environmental stress
- Are not present in the adult plants
 GMO-free
 Sustainable eco-friendly
 Allows utilization of even the most rare plant species
without harm
 Free of heavy metals, pesticides, and other
environmental contaminants
 No limitation of plant sources due to any restrictions
 Rare active molecules of interest can be concentrated
up
 Can be developed exclusively to meet one’s needs
 Safe for both cosmetic and nutritional applications
Plant Stem Cell Technological Process (Example)
Taking away a small piece of the plan
Wounding of plant material to induce callus formation
Incubation on agar plates
Harvesting of developed callus
Cultivation until complete dedifferentiation to obtain
stem cells
Transfer of the stem
cells into
into plants
a suspension and grow
Growing
 Are compatible with human skin
 Protect human skin stem cells
 Maintains the self renewal capacity of human skin
stem cells
 Increase longevity of human skin stem cells
 Replenish, regenerate and repair damaged DNA
 Delay aging of essential cells
 Stimulate duplication of human skin stem cells
 Protect the skin from ozone/pollution damage and
UV damage
Origin
Trade name
- Panax ginseng (Wild
Korean ginseng)
- ActostemR WKGBASE
- ActosomeR GINLIFT
- Cyperus papyrus
(Papyrus)
- Crodarom Papyrus
- Buddleja davidii
(Summer lilac)
- Centella asiatica (Asiatic
pennywort)
- Echinacea angustifolia
(Echinacea)
- Leontopodium alpinum
(Edelweiss)
- Buddleja davidii Stems GTM
Source
ACT, Korea
Crodarom,
France
- Centella asiatica Stems GTM
- Echinacea Angustifolia Stems GTM
- Leontopodium Alpinum Stems GTM
-Rhododendron
-PhytoCellTectTM Alp Rose
ferrugiineum (Swiss Alp
rose)
- Malus Domestica (Apple) - PhytoCellTecTM Malus Domestica
- Vitis Vinifera (Grape)
- PhytoCellTecTM Solar Vitis
IRB, Italy
Mibelle
Biochemistry,
Switzerland
Rhododendron Increase skin cell vitality; boost
ferrugiineum epithelial regeneration; help
skin to cope with climate
changes
Buddleja
Protect photodamage and
davidii
oxidative stress; offer complete
treatment for acne disorders
Leontopodium Reduce eye contour and
alpinum
collagen loss; prevent
photoaging
Echinacea
Promote collagen; revitalize
angustifolia
dull, tired skin
Malus
Domestica
Preserve the youthful look and
the vitality of skin
Vitis Vinifera
Protect skin stem cells against
UV stress, fight photoaging
Centella
Asiatica
Firm skin; improve skin tone and
elasticity
Cyperus
papyrus
Moisturize, soothe and energize
the skin
Pinax ginseng Reduce wrinkles; inhibit adipocyte differentiation
Edelweiss
Grows spontaneously on mountains
range from Pyrenees and Alps to Himalaya
Develop many defensive mechanisms in
order to survive the extreme heights and
protect from high level exposure to solar
radiations
From Edelweiss, the symbol of
“Alpine flora” with the aid of High
Tech Nature (HTN) biotechnologoy
HTN biotechnology is the unique process that
allows the in vitro culture of plant stem cells in
highly controlled conditions and enables to
produce the active substances with several innate
advantages :
- be able to produce substances that are rare
and difficult to obtain from nature or synthesis
- secure the growing stem cells from pesticides,
heavy metals and aflatoxins
- possibly obtain stems cells from endangered or
Callus
protected plants without altering the ecological
balance and biodiversity
- supply plant products independent of climatic
and geographical variability
contains specific
substances with high
biological activities
- Leontopodic acids A
and B, chlorogenic acid,
3,5-dicaffeoylguinic acid :
antioxidant, anti-hyaluronidasic, anti-collagenasic
Cosmetic Use
activity
> Reduction
of eye contour wrinkle
- Phytosterols,
amino
> Prevention of :collagen loss
acids, polysaccharides
> Firming and restructuring treatments
moisturizing and nutrients
> Prevention of aging and photo-aging
For Health
An
Apple aapple
Day Keeps
thetechnology
Doctor Away
Currently,
stem cell
is
popping up everywhere
for beauty use
For Beauty
An Apple a Day Keeps the Wrinkles Away
Apple Stem Cells as Anti-Aging
Ingredient
- The current find is a rare Swiss
apple, called the Uttwiler
Spatlauber, which actually heals
itself when punctured
- The rare Swiss apple has a
renewal power that rejuvenates
saggy skin
- Apple stem cells help boost the
production of human skin stem
cells by protecting skin from
stress and decreasing fine lines
and wrinkles
From Uttwiler Spätlauber, the
“super apple” with the aid of
modern plant stem cell technology
Selecting / taking away a small piece of the plant
Wounding of plant material to induce callus formation
Incubation on agar plates
Harvesting of the developed callus
Cultivation until complete dedifferentiation to obtain stem cells
Transfer of the stem cells into liquid media for growing
Disruption of the stem cell wall to release secondary metabolites
Encapsulation of the stem cell contents into liposome
Fixing in xanthan gum gel
- Based on an extract of cultured apple
stem cells
- Formulated with nanotechnology that
allows a higher encapsulation in a
smaller molecule
- Allows better penetration to deliver
deep into the skin
- Minimizes trans epidermal water loss
- Enhances new stem cell production
- Protect against stem cells from UV
- Released by skin heat (33oC) when is
applied
Encapsulated nanoemulsion PhytoCellTec
stem cell cream before
touching the skin
Heat activated release
of PhytoCellTec stem
cell cream after
touching the skin
Potentials
umbilical
PotentialsofofMalus
Malusdomestica
domesticastem
stemcell
cellextract
extractininprotecting
increasinghuman
the number
cord
stem cells
from cord
the impact
of UV
of human
umbilical
stem cells
Skin stem cell activity
Before PhytoCellTecTM treatment
1-h after PhytoCellTecTM treatment
- Won the 2008 innovation prize for best active
ingredient in Europe
- The reason it won is because its revolutionary
of stem cell technology and its ability to harness
the regenerative potential of own facial stem
cells to renew skin (make it look young again)
Cosmetic Use
>
>
>
>
>
Protect skin stem cells
Increase activity of skin stem cells
Decrease of senescence markers in fibroblasts
Maintain the power of skin cell regeneration
Decrease in wrinkles
55 years old
Week 1
Week 2
Week 3
Week 4
Potentials of Malus domestica stem cell extract on reducing of skin wrinkles
Korean Ginseng
 A medicinal herb with miraculous virtue in the
high mountains and secluded valleys
 A first record of prescription of ginseng as a
medicinal herb appeared in Shen-Neng
Pharmacopeia (AD 456-536)
 During the Koryo Dynasty (AD 918-1392),
ginseng was renowned as the best medicine with
miraculous potency
 It was called a miraculous medicine, a cureall and believed to be a miraculous medicine, a
source of eternal youth
From 100 years old Wild Korean
Ginseng (rare to find, even in
deep mountains) with tissue
culture technology
Non-GMO : no genetic modification
Safety : animal free, no-infection
Eco-friendly : no extraction, no pollution
No metals : no heavy metal sources
Pesticide free : no pesticide needed
Fast growth : no need to wait 100 years for
wild ginseng
Technology : tissue culture, nano-liposome
Low cost : low production cost
Genetic similarity by AFLP fingerprinting
Contains high biological substances
Cosmetic
Use
- Saponins called “ginsenosides” : protopana-
xadiol, triol
pleanolic
acid
 and
Collagen
synthesis
- Carbyhydrates : oligo- and polysaccharides,
 Cell proliferation
fiber, pectine
 soluble
Photo-protection
- Lipid
component : fat, phytosterol,
Antioxidant
essential 
oils,
terpenoids, organic acids
 Whitening
- Nitrogenous
compounds : Amino acids,
peptide, protein, alkaloid, nucleic acid
- Vitamins & minerals
Oh My God Perfect Gel
Japan
IOPE Plant Stem Cell Skin Renewal Serum
Korea
Bielenda In Vitro Roslinne Komorki Macierzvste
Anti-Age Day Cream SPF 10
Poland
éclos Anti-Aging Facial Cleanser Skin Prep
Rampal Latour en Provence Geneviève Patou
Shampoo & Shower Gel Sweet Almond - Sage
Bergamot
Crescina Stem Forte Re-Growth
USA
France
Italy
IOPE Plant Stem Cell Skin Renewal
Serum
IOPE's cutting edge technology
brings an innovative anti-aging
skin care line. Plant stem cells
are the source of unlimited
energy and the key to herbal
growth and regeneration
Bielenda In Vitro Roslinne Komorki
Macierzvste Anti-Age Day Cream SPF 10
Effectively repairs age-related skin
damage, restores the skin's firmness
and smoothness, reduces deep
wrinkles, furrows and mimic lines,
provides intensive hydration and
protects the skin against excessive
water loss, whilst restoring elasticity
and glow and improving the skin's
suppleness and density with its lifting
effect. Formulated with a plant stem
cells that naturally regenerate and
renew, protect skin stem cells against
ageing by delivering nutrients essential
for the formation of new, young cells.
The stem cells stimulate the
production of collagen, reduce wrinkles
and recover the skin's firmness and
youth.
éclos Anti-Aging Facial Cleanser Skin
Prep
Contains high potency plant
stem cells for cellular
rejuvenation. To extend skin
cell longevity, cleanse deep
down, and gently purify
pores. Clinical results give a
100% reported decrease in
wrinkle depth due to the
product's tremendous ability
to stimulate skin stem cells,
encouraging aging skin to
behave in a more youthful
way.
Lancôme Définicils Precious Cells Mascara
- Contains the apple extract blended with
proteins and lipids for a deeply hydrating
experience
- The potential in stimulating the growth
and health of lashes
Plant stem cells :
♥ are natural and free from many
unwanted issues e.g., GMO, environmental
contaminants
♥ contains substances that can be
beneficial for human health and beauty
♥ Can be produced by modern technology
and encapsulated for better properties
♥ Are compatible to human skin and
support skin stem cells as well as help
protect the skin from environmental stress
♥ are now available as the cosmetic
ingredients in the market
♥ have been used worldwide in skin care
and make up products
Sunee Chongcharoenchit
Siriluk Sreepan
Merck (Thailand) Ltd.
P.C. Intertrade Co., Ltd
Chemico Inter Corporation Co., Ltd
Croda Singapore PTE Ltd (Thailand
Representative Office)