Mendelian Genetics Professor Janaki Natalie Parikh jnparikh@verizon.net Human Traits- Tongue Rolling • Let’s try a human trait: Tongue Rolling • Note on conventions: typically use a capital letter to denote dominant & lwr case for recessive allele • Letter “R” used for tongue rolling (arbitrary) • 2 alleles: R, r inherited as a dominant trait • Genotypes: Phenotypes RR tongue roller Rr tongue roller rr non roller, can’t tongue roll Human Traits- Tongue Rolling • Punnett square scenario: couple considering having kids, he’s a tong. Roller & she’s not, will their kids be able to roll? (Given: his mom is not a roller) (rememb. 1st step) • ♀ & ♂ genotypes? • ♀: rr, & ♂ must be Rr? Why? Since his mom cannot roll, she must be rr). Now to setup the square… r R Rr rr r Now, let’s interpret… r Rr rr Human Traits Dimples: not these, but, rather, these Letter D is utilized, inherited as a recessive 2 alleles: D, d. Combos: DD, Dd, dd Scenario: Couple, neither have dimples, but they want to know if their kids can inherit them (Given: his dad has dimples & her mom does too) • 1st step? • ♀: Dd ♂: Dd D d • • • • D d DDD Dd Dd dd (Interpret &…) Multi-allelic system • Ready to throw in an additional allele? • Multi-allelic system (genetic menage a trois • ABO blood group, only O is a recessive here, notice the effect this has on phenotypes: • AA: blood type A • AO: blood type A • BB: blood type B • BO: blood type B • OO: blood type O any more? • AB: blood type AB Blood Groups • Relationship btwn A & B is such that it creates a reaction (agglutination), thus a person w/ A cannot be given B & vice versa • Thus AB blood type has an advantage, known as? • Universal Recipient, can be given any of the types • & type O is important to commty, known as? • Universal Donor, utilized in emergencies when typing cannot be done • Extra credit: https://www.givelife.org/index_flash.cfm Blood Type Punnetts • Scenario: A woman has a baby & has identified 2 men as potential fathers. Can we rule anyone out based on blood type? ♀: blood type O, Baby: blood type A, ♂ #1: blood type AB, ♂ #2: blood type O • Genotypes? • ♀: OO, ♂ #1: AB, ♂#2: OO A O O Interpret: B AO BO AO BO O O O O OO OO OO OO Chromosomes & Reproduction • Cells: basic unit of life • Organelles: carry out a specific function • Our focus: • Nucleus: DNA is housed inside it • Mitochondria: Pwrhouse • Inherited from whom? • Ribosome: site of protein manufacture Chromosomes human cells are eukaryotic: DNA in the nucleus DNA packaged into chromosomes Human cells have how many chromosomes? 46 chromosomes in 23 homologous pairs Chromosome # is arbitrary, not related to sz., complexity or intelligence • Ex: Mosquito: 6 chrom. • White Oak Tree: 24 chrom. • Turkey: 82 chrom. • • • • • Chromosomes • • • • • Gene locus (loci): specific location of a gene Homologous pairs: corresponding gene loci Per pair, 1 chromosome from each parent Pairs 1-22: autosomes Pair 23: sex chromosomes Mitosis a.k.a. Cell Division a.k.a. asexual reproduction Cells: somatic cells & gametes Somatic cells: diploid chrom. #46 Gamete,sex cell: haploid chrom #23 Somatic cells replenished via mitosis 1 exception: once maturity reached, no more are made, what type? • Mitosis yields: 2 daughter cells, identical to orig. parent cell • • • • • Meiosis a.k.a production of gametes a.k.a. sexual reproduction • Begins w/ a diploid cell • However, cleavage division takes place twice • Notice: DNA replication only happens once • Meiosis results: 4 end products: haploid gametes • This specific example must be spermatogenesis Spermatogenesis • Why? The other type of meiosis (oogenesis) plays out difftly, sperm only requires genetic info, sugar & a tail for motility • Results: Spermatogenesis: 4 haploid gametes • Where does this process take place? • In the testes. When does it begin? • W/ the onset of puberty, prior to that no sperm are produced. Oogenesis • Oogenesis: production of oocytes. Involves an an assymetric cleavage division. The smaller section is referred to as a polar body. Results: each full process only yields 1 viable oocyte & 3 polar bodies, which are genetic waste Oogenesis • Where does this take place? • In the ovaries. When? • Previously thought solely in utero, new evidence suggests that it continues during a ♀s lifetime • Why is oogenesis so difft? • In addition to genetic info, oocytes also require organelles to sustain life. Each living thing inherits its orig. set of organelles from mom’s oocyte. Thus, mitochondria (which have their own separate DNA) are passed on from mother to her offspring Mendelian Principles • Princ. Of Segregation: in the production of gametes, homologous chromosomes segregate into separate gametes, a gamete will receive only 1 of the 2 homologous chromosomes • Princ. Of Independent Assortment: whichever of 2 homologous chromosmes is contributed to a gamete is independent of whichever of any 2 other homologous chromosomes that is contributed to the same gamete Mendelian Principles Mendelian Principles Genetic Recombination • These 2 principles contribute to a seemingly endless # of possible combinations of the same orig. set of genetic material: genetic recombination,the reason why you & your siblings don’t look alike Sex Chromosomes • Like the autosomes, code for norm. body traits, however, also determine a person’s biological sex • Size differential btwn the 2 sex chromosomes • Typical pairings: XX, yielding? • Biological female. & XY, yielding? • Biological male • As a result, there are many alleles located on the X chromosome for which the Y does not provide a corresponding allele http://www.newscientist.com/article/dn12971-diet-may-influenceyour-babys-gender.html Sex Linked Traits • Due to sz. diffc. there are many traits for which ♂s only inherit 1 single allele, located on their X chromosome • These traits are called “sex-linked” or “X-linked” • For sex linked traits, ♂s are hemizygous, meaning they have only 1 allele XY: ♂ XX: ♀ Sex Linked Traits • This also makes them more susceptible to X linked recessive conditions • Normally, to inherit a recessive condition, requires how many alleles? • 2, one from ea. parent. Not for sex linked traits • What are some examples? • Pattern balding, red-green color blindness, hemophilia, fragile X syndrome, etc… • http://anthro.palomar.edu/biobasis/bio_4.htm Human Traits • http://www.nerdmodo.com/wp-content/uploads/2009/05/5391701_std11.jpg