Campbell and Reece 10 th Edition Medium Ground Finch from island of Daphne Major in Galápagos Islands Long period of Individuals do not evolve, populations do drought altered their food supply to mostly larger nuts & over the years those individuals with larger beaks were more successful Average beak size & size of individual birds larger after the drought so…. The medium ground finch population had evolved by natural selection Darwin reasoned that natural selection acted on genetic variation of populations He knew nothing about genes Few yrs later: Mendel’s paper on inheritance in pea plants: stage set for understanding variation Genotype inheritable, phenotypes are not Example: moth, Nemoria arizonaria, appears very different eating oak flowers vs. oak leaves In general, only the genetically determined part of a phenotype can affect evolution Discrete characters = “either/or” ( Mendel’s pea) = single gene Most heritable variations involve quantitative characters: vary along a continuum ≥ 2 genes way to quantify gene variability average % of loci that are heterozygous can calculate average: turns out if the average heterozygosity is 14% there is enough genetic variation for natural selection to act evolutionary change does not show silent mutations (DNA changes but still codes for same a.a.) Geographic Variation differences in genetic composition of separate populations Mutation Gene Duplication Sexual Reproduction Other process that results in new alleles or new genes Organisms with short life spans new genetic variants arise fairly rapidly 1. Mutations can’t predict where in genome or what type mutation for multicellular organisms only mutations in gametes cell line passed to new generations (most are in somatic cell line) most point mutations silent or only slightly harmful, rarely are they beneficial chromosomal changes that delete, disrupt, or rearrange usually lethal or harmful if genes left intact they may be neutral changes Translocation: Part of 1 chromosome breaks off & attaches to another chromosome if large segments duplicated usually harmful duplications of small pieces may be beneficial mutations accumulate over time eventually that duplication takes on new role end result: expanded genome average mutation rate in plants & animals is considered low ~ 1 mutation in every 100,000 genes / generation shorter generation spans allows for generation of genetic variation in a population virus populations, especially retroviruses process is fastest single stranded RNA: less complicated to duplicate fewer RNA repair mechanisms in host cells most effective treatment for a quickly mutating retrovirus has been combination protocols most of genetic variation due to crossing over and independent assortment of chromosomes in meiosis and fertilization 1 of 3 factors that presence of genetic variation does not guarantee that population is evolving cause evolution must be at work in a population Population: group of same species in same area that interbreed, with fertile offspring examples of isolated populations: Islands Lakes even populations not strictly isolated members tend to breed with own population so are genetically closer to them than other groups consists of all copies of every allele at every locus in all members of a population if there is only 1 allele for a locus that allele is said to be fixed in the gene pool; entire population is homozygous for that gene if there are 2 or more alleles for a locus then individuals may be homozygous or heterozygous to test whether natural selection is acting on a particular locus: Determine what the frequency would be if it were not evolving Then compare that calculation with what you measure in the population No difference: not evolving difference: evolving Hardy Weinberg states that the frequencies of alleles & genotypes in a population will remain constant from generation to generation, provided that only Mendelian segregation & recombination of alleles are at work If that is true the population is said to be in HARDY-WEINBERG EQUILIBRIUM http://nhscience.lonestar.edu/biol/hwe/q1d.html http://www.phschool.com/science/biology_place/labb ench/lab8/intro.html Problem 2: If 9% of an African population is born with a severe form of sickle cell anemia (ss) what % of the population will be more resistant to malaria because they are heterozygous (Ss) for the sickle-cell gene? 2pq = 2 (.7 x .3) = .42 = 42% of the population are heterozyotes (carriers) 1. No Mutations 2. Random Mating 3. No Natural Selection 4. Extremely Large Populations 5. No Gene Flow Departure from any of the 5 conditions usually results in evolutionary changes A population may be evolving at some gene loci and in Hardy-Weinberg Equilibrium at other loci Can be used to estimate the frequency of a gene causing inherited disease in a population Must assume: No new mutations Random mating Ignore any effects of differential survival & reproductive success No genetic drift 1. 2. 3. 4. 5. No Mutations: not usually significant unless mutation produces new alleles that have a strong influence in a (+) or (-) way Random Mating: not usually significant No Natural Selection cause most Extremely Large Populations evolutionary No Gene Flow change is based on differential success in survival & reproduction if NS consistently favoring some alleles over others, NS can cause adaptive evolution (dfn: evolution that results in a better match between organisms & their environment) process in which chance events cause unpredictable fluctuations in allele frequencies from one generation to the next the smaller the population the more pronounced the effect 1. Founder Effect genetic drift that occurs when a few individuals become isolated from a larger population & form a new population whose gene pool composition is not reflective of original population 1 colonist carried recessive allele for retinitis pigmentosa By late 1960’s, there were 240 descendants of the original founders 4 had retinitis pigmentosa This frequency is 10x higher than frequency of retinitis pigmentosa in England 2. Bottleneck Effect: occurs when the size of a population is reduced, as by a natural disaster or human actions. The resulting population is genetically different than original population. Genetic Drift : 1. is significant in small populations 2. can cause allele frequencies to change at random 3. can lead to a loss of genetic variation w/in populations 4. can cause harmful alleles to become fixed the transfer of alleles from one population to another as result of movement of fertile individuals or their gametes transferred alleles may increase a population’s ability to adapt to local conditions Culex pipiens spread of insecticideresistant alleles used to treat mosquitoes to prevent spread of West Nile outcome of NS is not random NS increases frequencies of alleles that provide reproductive advantage so, leads to adaptive evolution NS acts directly on the phenotype & indirectly on the genotype the contribution an individual makes to the gene pool of the next generation, relative to the contribution of other individuals in the population 1. DIRECTIONAL SELECTION conditions favor individuals favoring one extreme of a phenotype shifts curve in one direction or other 2. DISRUPTIVE SELECTION conditions favor individuals at both extremes of a phenotypic range over individuals with intermediate phenotypes 3. STABILIZING SELECTION conditions favor the intermediate phenotype and act against both extremes reduces variation Natural selection will increase the frequencies of alleles that enhances survival & reproduction so… over time adaptations arise genetic drift & gene flow may cause changes that are either advantageous or disastrous individuals with certain inherited characteristics are more likely to obtain mates can result in sexual dimorphism: marked differences between the 2• sex characteristics 1. Intrasexual Selection selection w/in same sex Alpha male 2. Intersexual Selection aka mate choice females choosy about their mate (often depends on male showiness) 1 hypothesis: females have linked “good genes” with trait study: gray tree frog 2nd hypothesis females have linked “good health” with these traits study: birds neutral variation: differences in DNA that do not confer an advantage or disadvantage Why don’t all genes move toward neutrality? tendency for directional or stabilizing selection countered by mechanisms that preserve or restore variation recessive alleles hidden and carried forward in heterozygotes heterozygote protection maintains a huge pool of alleles that might not be favored under present conditions, but could bring benefits in environment changes occurs when natural selection maintains 2 or more forms in a population 2 types: heterozygote advantage 2. frequency-dependent selection 1. Heterozygotes have survival advantage If phenotype of a heterozygote is intermediate between the 2 homozygotes then this advantage is: stabilizing selection If phenotype of heterozygote same as dominant homozygote this advantage is directional selection Example: Sickle Cell SS homozygous dominant no protection against malaria Ss heterozygous protection against malaria few sickle cells but not harmful ss homozygous recessive die young of sickle cell the fitness of a phenotype depends on how common it is in the population eat scales off flank of prey some leftmouthed some right-mouthed right-mouthed dominant to leftmouthed selection favors whichever mouth phenotype is least common (prey fish learn to avoid attacks from more common 1. Selection can only act on existing variations NS favors only the fittest available phenotypes 2. Evolution is limited by historical constraints NS has to work with existing structures 3. Adaptations are often compromises each organisms must do many things: some structures are a compromise (Walrus fins great for swim, not so good for walking on rocks) 4. Chance, natural selection, & the environment interact founding population may not carry “best” alleles for new environment; environments can change