Cystatin C, Lipoprotein(a) and
Ceruloplasmin
in the Thermo Scientific
Konelab Test Menu
Specific Protein tests on Konelab Systems
Albumin
Antistreptolysin O
 -1-Antitrypsin
Apo A1 & B
Complement C3
Complement C4
C-reactive protein
High Sensitive CRP
HbA1c
Haptoglobin
IgA, G & M
Microalbuminuria
Myoglobin
Orosomucoid
Prealbumin
Rheumatoid factors
Transferrin
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• Cystatin C
• Lipoprotein(a)
• Ceruloplasmin
Renal testing
• Creatinine, Enzymatic
• Creatinine, Jaffé
• Urea/BUN
• Total protein in urine
• Microalbuminuria
• Cystatin C
Source: http://training.seer.cancer.gov/module_anatomy/unit11_2_uri_components.html
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Cystatin C - a Novel Serum Marker for GFR
• Kidney monitoring
• GFR, Glomerular Filtration Rate
• Traditionally with Creatinine measurement
• NEW MARKER – CYSTATIN C
• Why Cystatin C together or instead of Creatinine?
• Specificity
• Creatinine is affected by age/sex/muscle mass/inflammatory
process
• Cystatin C test is not dependent of any listed issues
• Sensitivity
• Creatinine measurement is able to detect kidney dysfunction
when app. 50% of kidney mass is ‘non-working’
• Cystatin C test has the sensitivity to detect already mild renal
dysfunction
• Other highlights
• Elevated serum Cystatin C is a strong predictor of the risk of
death and cardiovascular events in elderly persons
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Cystatin C on Konelab systems
• Easy and reliable assay
• Ready for use liquid reagents
•
•
•
•
Method: latex enhanced immunoturbidimetry
On board stability for reagents 30 days
Kit size 2 x 3 ml, up to 190 tests
981911 Cystatin C
• One level stock calibrator
•
•
•
•
Ready for use liquid
Automatic dilutions for calibration curve
Kit size 2 x 1 ml
981912 Cystatin C Calibrator
• Controls
• Normal and elevated levels
• 981913 Cystatin C Control, 3 x 1 ml
• 981914 Cystatin C Control High, 3 x 1 ml
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Lipid tests
• Total Cholesterol
• HDL & LDL-Cholesterol
• Triglycerides
• Apolipoprotein A1 & B
• Lipoprotein(a)
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Classification of Lipids
• Sterols: Cholesterol, steroid hormones etc.
• Fatty acids
• Glycerol esters, e.g. triglycerides
• Sphingosines and Terpenes
• e.g. Vitamin A, E and K
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Cholesterol
• Synthesized in liver
• 10% coming from dietary intake
• Transported in complexes known as lipoproteins
• Clinical interest in HDL and LDL Cholesterol
Triglyceride
+
Esterified
Cholesterol
Apolipoprotein
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Lipoproteins
• Apolipoprotein A-I/II => HDL: “good cholesterol”
• Apolipoprotein B => LDL: “bad cholesterol”
• Lipoprotein(a) = LDL- molecule + Apolipoprotein (a)
Triglyceride
+
Esterified
Cholesterol
Apolipoprotein
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Lipoprotein(a)
• A powerful predictor for development of
atherosclerotic vascular disease
• Lipoprotein(a) is a dimer consisting of LDL
molecule bound to Apo(a) via a disulphide
bridge
• Lp(a) is a risk factor for coronary heart
disease (CHD) that is independent of all other
lipid parameters
• Lp(a) is a special tool for doctors
• The CHD risk predictor is high, especially
when Lp(a) and LDL are elevated
simultaneously => lipid lowering therapy
recommended
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Lipoprotein(a) –
a ’killer molecule’
Lipoprotein(a) on Konelab Systems
• Easy and reliable assays
• Ready for use liquid reagents
• Method: direct immunoturbidimetry
• Kit size 1 x 1 ml, up to 180 tests
• 981915 Lipoprotein(a)
• One level stock calibrator
• Ready for use liquid
• Automatic dilutions for calibration curve
• 981916 Lipoprotein(a) Calibrator, 2 x 1 ml
• Controls
• Normal and elevated levels
• 981917 Lipoprotein(a) Control, 3 x 1 ml
• 981918 Lipoprotein(a) Control High, 3 x 1 ml
k
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Ceruloplasmin
• Ceruloplasmin measurement aids to diagnose copper
metabolism disorders
• Ceruloplasmin is the major copper–containing protein in
plasma
• When is the Ceruloplasmin level abnormal?
• Decreased levels
• disorders of copper metabolism like Menke’s disease
and Wilson’s disease
• Elevated levels
• During pregnancy
• With lymphoma
• In acute and chronic infections (acute phase protein)
• Rheumatoid arthritis
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Ceruloplasmin on Konelab Systems
• Ready for use liquid reagents
•
•
•
•
Method: direct immunoturbidimetry
On board stability for reagents 30 days
Kit size 2 x 3 ml, up to 190 tests
981919 Ceruloplasmin
• SpeciCal for calibration
• Ready for use liquid
• Automatic dilutions for calibration curve
• 980997 SpeciCal, 5 x 1 ml
• Controls
• 981250 SpeciTrol, 5 x 1 ml
• 981799 SpeciTrol High, 5 x 1 ml
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Albumin
Tot & Dir Bil
Bicarbonate
Calcium
Cholesterol
HDL&LDL Chol
Creatinine
Glucose
Iron
Magnesium
Phosphorus
Total Protein
Triglycerides Lipase
U/CSF Protein
Urea
Uric Acid
ENZYMES
SUBSTRATES
Konelab Menu – over 80 carefully validated methods
ACP
ALP
ALT/GPT
AST/GOT
Amylase
CHE
Dibucaine
CK
CK-MB
Gamma-GT
-HBDH
LDH
ELECTROLYTES
Na, K, Cl
Ca, pH
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Amphetamine
Barbiturates
Benzodiazepines
Cannabinoids
Cocaine Metabolite
Opiates
Cotinine
Ethyl Alcohol
Methadone
Propoxyphene
TDM
Albumin
Antistreptolysin O
 -1-Antitrypsin
Apo A1 & B
Complement C3
Complement C4
C-reactive protein
High Sensitive CRP
HbA1c
Haptoglobin
IgA, G & M
Microalbuminuria
Myoglobin
Orosomucoid
Prealbumin
Rheumatoid factors
Transferrin
sTfR
Cystatin C
Lipoprotein(a)
Ceruloplasmin
DoA (DAT)
SPECIFIC PROTEINS
Konelab Menu – over 80 carefully validated methods
Carbamazepine
Phenobarbital
Phenytoin
Theophylline
Valproic Acid
Lithium
Konelab System Reagents
• Proven, continously expanding
test menu
• Delivered in optimal package sizes to
serve different sized laboratories
• Ready for use liquid reagents for
ease of use
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