Analysis of Covariance, ANCOVA (GLM2) Slide 1 Aims • • • • When and Why do we use ANCOVA? Partitioning Variance Carrying out on PASW/SPSS Interpretation – Main Effects – Covariates Slide 2 When And Why • To test for differences between group means when we know that an extraneous variable affects the outcome variable. • Used to control known extraneous variables. Slide 3 Advantages of ANCOVA • Reduces Error Variance – By explaining some of the unexplained variance (SSR) the error variance in the model can be reduced. • Greater Experimental Control: – By controlling known extraneous variables, we gain greater insight into the effect of the predictor variable(s). Slide 4 Variance SST Total Variance In The Data SSM SSR Improvement Due to the Model Error in Model Covariate Slide 5 SSR An Example • We will use Field’s (2009) Viagra example (from the ANOVA lecture). – There are several possible confounding variables – e.g. Partner’s libido, medication. • We can conduct the same study but measure partner’s libido over the same time period following the dose of Viagra. – Outcome (or DV) = Participant’s libido – Predictor (or IV) = Dose of Viagra (Placebo, Low & High) – Covariate = Partner’s libido Slide 6 Relationships between the IV and Covariate Homogeneity of Regression Slopes Slide 9 Dose Participant’s Libido Partner’s Libido Placebo 3.22 (1.79) 3.44 (2.07) Low Dose 4.88 (1.46) 3.12 (1.73) High Dose 4.85 (2.12) 2.00 (1.63) How Does ANCOVA Work? • Imagine we had just two groups: – Placebo – Low Dose • This paradigm can be expressed as a regression equation using a dummy coding variable: Yi b0 b1Xi Libidoi b0 b1Dos ei Slide 10 Dummy Coding • Dummy Coding – Placebo = 0, Low Dose = 1 – When Dose = Placebo, Predicted Libido = mean of placebo group: X Placebo b1 0 b0 X Placebo b0 – When Dose = Low Dose, Predicted Libido = mean of Low Dose group: X b 1 b LowDose 1 0 X LowDose b1 X Placebo X LowDose X Placebo b1 Slide 11 ANOVA As Regression • We can run a regression with Libido as the outcome and the Dose (Placebo or Low) as the predictor, Note: – Intercept is the mean of Placebo group – b for the Dummy Variable is the difference between the means of the placebo and low dose group (4.88-3.22 = 1.66) Coefficientsa Model 1 (Constant) Dummy Variable 1 (Placebo vs. Low) a. Dependent Variable: Libido Slide 12 Unstandardized Coefficients B Std. Error 3.222 .547 1.653 .798 Standardized Coefficients Beta .472 t 5.888 Sig . .000 2.072 .056 ANCOVA • ANCOVA extends this basic idea. • The covariate can be added to the regression model of the ANOVA. • To evaluate the effect of the experimental manipulation controlling for the covariate we enter the covariate into the model first (think back to hierarchical regression). Yi b0 b1X i b2Covar iate Libidoi b0 b1Dos ei b2Par tners' Libidoi Slide 13 To Recap • To control for the effect of a covariate all we do is do a multiple regression in which we enter the covariate in the first step. • We enter Dose in a second step • The result is that we see the effect of dose above and beyond the effect of the covariate. Coefficientsa Model 1 2 (Constant) Partner's Libido (Constant) Partner's Libido Dummy Variable 1 (Placebo vs. Low) a. Dependent Variable: Libido Slide 14 Unstandardized Coefficients B Std. Error .421 .534 .596 .082 -.362 .440 .872 .094 -1.847 .487 Standardized Coefficients Beta 1.291 .788 7.293 -.824 9.295 Sig . .443 .000 .424 .000 -.527 -3.795 .002 .883 t Dose Placebo Low Dose High Dose Slide 15 Participant’s Libido Partner’s Libido 3 2 5 2 2 2 7 2 4 7 5 3 4 4 7 5 4 9 2 6 3 4 4 4 6 4 6 2 8 5 4 1 5 1 2 2 7 4 5 5 3 1 2 2 6 4 2 1 3 5 4 3 3 2 0 1 3 0 1 0 5 4 3.22 4.88 4.85 Low High 1.66 3 2 1 0 Placebo Slide 16 ANCOVA on SPSS Slide 17 Contrasts Slide 18 Options Slide 19 Without the Covariate Tests of Between-Subjects Effects Dependent Variable: Libido Source Corrected Model Intercept DOSE Error Total Corrected Total Type III Sum of Squares 16.844a 535.184 16.844 94.123 683.000 110.967 df 2 1 2 27 30 29 Mean Square 8.422 535.184 8.422 3.486 a. R Squared = .152 (Adjusted R Sq uared = .089) Slide 20 F 2.416 153.522 2.416 Sig . .108 .000 .108 Output Levene's Test of Equality of Error Variancesa Dependent Variable: Libido F 5.525 df1 df2 2 27 Sig . .010 Tests the null hypothesis that the error variance of the dependent variable is equal across g roups. a. Design: Intercept+PARTNER+DOSE Tests of Between-Subjects Effects Dependent Variable: Libido Source Corrected Model Intercept PARTNER DOSE Error Total Corrected Total Type III Sum of Squares 34.750a 12.171 17.906 28.337 76.216 683.000 110.967 df 3 1 1 2 26 30 29 Mean Square 11.583 12.171 17.906 14.169 2.931 a. R Squared = .313 (Adjusted R Sq uared = .234) Slide 21 F 3.952 4.152 6.109 4.833 Sig . .019 .052 .020 .016 SPSS Output: Contrasts Slide 22 Output Slide 23 Unadjusted Means 5 4 3.22 4.88 4.88 4.85 4.85 Low High 3.22 3 2 1 0 Placebo Slide 24 The Main Effect 10 8 6 5.15 4.71 2.93 4 2 0 Placebo Low High F(2, 26) = 4.14, p < .05 Slide 25 The Covariate F(1, 26) = 4.96, p < .05 Slide 26